BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0015.p (513 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4F265.1 A4F265_9BACL Putative sugar ABC transporter 497 e-138 B1DHI4.1 B1DHI4_9BACL Extracellular solute-binding protein family 1 491 e-137 B3K541.1 B3K541_9BACI Extracellular solute-binding protein family 1 480 e-133 Q5WK42.1 Q5WK42_BACSK Sugar ABC transporter substrate-binding pr... 432 e-119 C3J6V6.1 C3J6V6_9BACI Extracellular solute-binding protein family 1 355 7e-96 >A4F265.1 A4F265_9BACL Putative sugar ABC transporter Length = 545 Score = 497 bits (1279), Expect = e-138, Method: Compositional matrix adjust. Identities = 246/539 (45%), Positives = 344/539 (63%), Gaps = 34/539 (6%) Query: 1 LSGCAFRSTGESLPAASPESTAPHFDVKTGKYNPPVDLYTVGSVNPNLTFKKGESLEHNV 60 LSGC + E A+P + + ++K GKY+PPV L + P + FK GE ++NV Sbjct: 8 LSGCNSGAGKE----AAPSTPSTALELKDGKYDPPVKLTYLRPWGPEIKFKSGEDQDNNV 63 Query: 61 HTIWAENRLGIRIRYLWTISGTSETYANKLRLELA-KGNMPDVVTTR-DANVIQELIDSG 118 HT WA+++LGI ++ W T+ + KLRL LA MPD+++ R D N+ +ELI++G Sbjct: 64 HTKWAKDKLGIELKNQWVSPSTNNAFETKLRLSLASNAEMPDIISYRGDFNLARELIETG 123 Query: 119 QFMEAGSLFGQFASKVWKQAVAEDPSAWDSYVRDGHKYAIPIMDYEYNSDPLLWIRQDWL 178 +F++AG LF ++AS WK AV EDPS W Y++DG + IPI+DY YNSDP++WIR+DW+ Sbjct: 124 KFVDAGELFDKYASDTWKAAVNEDPSVWYPYMQDGKRIGIPILDYSYNSDPVMWIREDWM 183 Query: 179 DKLHLSAPRTLDELEQVMDAFVNRDPDGNGLKDTYGLAVGFRNGPSTWMGDSSWIFGAFG 238 K +L AP TL +LE +M+ F N+DPDGNG KDTYGL +GF+N STWM D+ W+FGA+G Sbjct: 184 KKFNLKAPETLADLEVIMETFTNKDPDGNGKKDTYGLTIGFKNWLSTWMSDAGWVFGAYG 243 Query: 239 TVPEQWNRRSDGTLEYGSVQPGARKAVALMKQWVQQGYLSTDSAWLDEEGAANLFVSGKA 298 T+P QWN ++G LEYGSV PGA++A+A + W+++GY+ ++ DE AA F +GKA Sbjct: 244 TMPNQWNLNAEGKLEYGSVNPGAKQALATLADWMKKGYIPEEAGVYDETKAAEEFTAGKA 303 Query: 299 GIIAGPYWMGGWPLSHLTSADPQARIKAAAIPAGPDGTVMRRGTLPVNGAILINKKMMHP 358 GI+ GP+WM WPL + +P+A KA IP+GPDG RRGT NG +LINK M +P Sbjct: 304 GIVVGPHWMPSWPLEDVKKNNPEAEYKAYPIPSGPDGKAGRRGTANDNGVVLINKDMKNP 363 Query: 359 EIFFTYQNYLFDYYATSTG--EFINGLAKDYDWTMDAGQPTIRPSALPLGGIRVASYTLT 416 E+FFTYQNYLFD+YA EF NG A+ YDW M G+ + A GG YTLT Sbjct: 364 EVFFTYQNYLFDHYANPKAGDEFENGFAEGYDWAMKDGK--VPTDASVTGGYAPEKYTLT 421 Query: 417 FDGARIPSKVIKEIPK------------------------DIAPVLLSQKEASRKEQFTG 452 FDGARIPS + + K + A ++ QK+ + K+ FTG Sbjct: 422 FDGARIPSLNMTTLAKFANGEEATTPFEKKLKSGVPVPMVEAAKIVQDQKDIALKQMFTG 481 Query: 453 PPTATMKSDGELLKKLEQKSFQKIIFADSGIGEFDEFVDKWKAYGGLTETSEVNAWDRS 511 PT +M+ + ++L K+E+ F +II+ + + FD FV+KWK+ GG T+EVN W +S Sbjct: 482 APTMSMQMNNDILTKMEKDLFSQIIYGKAPVDAFDTFVEKWKSSGGDQNTNEVNEWYQS 540 >B1DHI4.1 B1DHI4_9BACL Extracellular solute-binding protein family 1 Length = 574 Score = 491 bits (1264), Expect = e-137, Method: Compositional matrix adjust. Identities = 241/514 (46%), Positives = 338/514 (65%), Gaps = 30/514 (5%) Query: 26 DVKTGKYNPPVDLYTVGSVNPNLTFKKGESLEHNVHTIWAENRLGIRIRYLWTISGTSET 85 + K K++PP+ + + P++ FK GE ++NVHT WA ++ GI+++ W T+ Sbjct: 57 EAKDVKFDPPITITYLRPWGPDVKFKSGEDQDNNVHTKWALDKFGIQLKNQWIAPSTNNA 116 Query: 86 YANKLRLELAKG-NMPDVVTTR-DANVIQELIDSGQFMEAGSLFGQFASKVWKQAVAEDP 143 + KLRL LA MPD+++ R D +++ELIDSG+F++AG LF ++AS+VWK A+ EDP Sbjct: 117 FETKLRLSLATNEKMPDIISYRGDFTLVRELIDSGRFVDAGELFDKYASQVWKDAMNEDP 176 Query: 144 SAWDSYVRDGHKYAIPIMDYEYNSDPLLWIRQDWLDKLHLSAPRTLDELEQVMDAFVNRD 203 + W Y+ DG + IPI+DY YN DP++WIR+DW+ KL L AP+TLD+LE+VMDAF N+D Sbjct: 177 TVWYPYMEDGKRIGIPILDYAYNGDPVMWIREDWMQKLGLPAPKTLDDLEKVMDAFTNQD 236 Query: 204 PDGNGLKDTYGLAVGFRNGPSTWMGDSSWIFGAFGTVPEQWNRRSDGTLEYGSVQPGARK 263 PDGNG KDTYGLA+GF+N +TWM D+ WIFGA+GT+P QWN+ +DG LEYGSV G++K Sbjct: 237 PDGNGKKDTYGLAIGFKNWINTWMSDAGWIFGAYGTMPNQWNKTADGKLEYGSVTEGSKK 296 Query: 264 AVALMKQWVQQGYLSTDSAWLDEEGAANLFVSGKAGIIAGPYWMGGWPLSHLTSADPQAR 323 A+A ++ W+ +GYL T++ DE AA F +GKAGI+ GP+WM WPL + + A+ Sbjct: 297 ALATLQSWMSKGYLPTEAGLYDETKAAEDFTAGKAGIVVGPHWMPAWPLEDVKKNNKDAQ 356 Query: 324 IKAAAIPAGPDGTVMRRGTLPVNGAILINKKMMHPEIFFTYQNYLFDYYAT--STGEFIN 381 KA +PAGPDG V R GT NG +LIN +M HPE FF YQNYLF+ YA + GEF N Sbjct: 357 YKAYPLPAGPDGKVGRHGTSNSNGVVLINAEMEHPEAFFAYQNYLFEQYANPQAGGEFEN 416 Query: 382 GLAKDYDWTMDAGQPTIRPSALPLGGIRVASYTLTFDGARIPSKVIKEIPK--------- 432 GLA+ YDW M GQ ++ A GG YTLTFDGARIPS ++ + K Sbjct: 417 GLAEGYDWVMVDGQ--VKTDAASTGGYAPEKYTLTFDGARIPSLNMQVLSKLANGEEATT 474 Query: 433 ---------------DIAPVLLSQKEASRKEQFTGPPTATMKSDGELLKKLEQKSFQKII 477 + A ++ QK++ + FTG PT TM++ ++L K+E+ +F +II Sbjct: 475 PFEKKLKLSSPQPLLEAAKIVQEQKDSVLPQMFTGAPTKTMQTSNDILGKMEKDTFSQII 534 Query: 478 FADSGIGEFDEFVDKWKAYGGLTETSEVNAWDRS 511 + + + FD+FV+KWK+ GG T EVN W +S Sbjct: 535 YGKAPVDSFDKFVEKWKSSGGDKITQEVNEWYQS 568 >B3K541.1 B3K541_9BACI Extracellular solute-binding protein family 1 Length = 561 Score = 480 bits (1236), Expect = e-133, Method: Compositional matrix adjust. Identities = 253/543 (46%), Positives = 337/543 (62%), Gaps = 33/543 (6%) Query: 1 LSGCAFRSTGESLP-AASPESTAPHFDVKTGKYNPPVDLYTVGSVNPNLTFKKGESLEHN 59 L+GC S GE P AA + + +V KY+P V + TV V+P L FK GE++E+N Sbjct: 22 LAGC---SGGEPKPEAAQQPAESKTENVYKEKYDPEVTITTVWGVDPTLQFKNGETIENN 78 Query: 60 VHTIWAENRLGIRIRYLWTISGTSETYANKLRLELAKGN-MPDVVT--TRDANVIQELID 116 V T WA+ + GI I+ LW+I+ T+ +A KLRL ++ G MPD+VT T + + Q+LID Sbjct: 79 VATKWAKEKFGINIKSLWSITDTNGAFATKLRLAMSSGQEMPDIVTLGTENQQLAQDLID 138 Query: 117 SGQFMEAGSLFGQFASKVWKQAVAEDPSAWDSYVRDGHKYAIPIMDYEYNSDPLLWIRQD 176 SG F E G+LF Q+AS+ W+ A+ DP+ W+ Y RDG + +P++D+ YN D +LWIRQD Sbjct: 139 SGMFAEVGTLFDQYASETWRNAMNLDPNVWNQYSRDGKRMGLPVLDFAYNHDYVLWIRQD 198 Query: 177 WLDKLHLSAPRTLDELEQVMDAFVNRDPDGNGLKDTYGLAVGFRNGPSTWMGDSSWIFGA 236 WLDKL +SAP+T+DELEQVMDAF N++P G L++GF+ STWMGD SWIFGA Sbjct: 199 WLDKLGMSAPKTMDELEQVMDAFKNQNPSGLAPDKVTPLSIGFKTSMSTWMGDPSWIFGA 258 Query: 237 FGTVPEQWNRRSDGTLEYGSVQPGARKAVALMKQWVQQGYLSTDSAWLDEEGAANLFVSG 296 +GT+P+QWN DG+LEYGSV G ++ + + +W+ +GY+ ++A DE A V+G Sbjct: 259 YGTLPQQWNVAEDGSLEYGSVHAGMKQGLVKLNEWMNKGYIPKEAALWDENKTAEPAVAG 318 Query: 297 KAGIIAGPYWMGGWPLSHLTSADPQARIKAAAIPAGPDGTVMRRGTLPVNGAILINKKMM 356 AGII GPYWM GWPL P+A K IPAGPDG MR GT NG LI+K M Sbjct: 319 TAGIIPGPYWMSGWPLIDTVKNAPEAVWKPVQIPAGPDGIAMRHGTQYTNGVTLIHKDME 378 Query: 357 HPEIFFTYQNYLFDYYATST--GEFINGLAKDYDWTMDAGQPTIRPSALPLGGIRVASYT 414 HPE FFTYQNYLFD +A S GEF NGL + YD+ +DA + + G + V Y Sbjct: 379 HPEAFFTYQNYLFDNFAVSKEGGEFANGLFEGYDFELDANGEMVPNDQIQGGYVNVVRYL 438 Query: 415 LTFDGARIPSKVIK-------------EIPKDI-----------APVLLSQKEASRKEQF 450 L DGARIP + +K + KD+ A VLLSQ+E S+K F Sbjct: 439 LVRDGARIPDEQMKALLKLAKGGKPESRLEKDVAVNYGKDTPAAAEVLLSQEEISKKNMF 498 Query: 451 TGPPTATMKSDGELLKKLEQKSFQKIIFADSGIGEFDEFVDKWKAYGGLTETSEVNAWDR 510 TGP TATMKS + L K+E ++ +II+ + FD FV+ WKA GG T EVN W + Sbjct: 499 TGPVTATMKSKWDYLLKIENQTLNEIIYGKQPVESFDTFVETWKAGGGEQITKEVNEWYQ 558 Query: 511 SRR 513 S + Sbjct: 559 SVK 561 >Q5WK42.1 Q5WK42_BACSK Sugar ABC transporter substrate-binding protein Length = 557 Score = 432 bits (1110), Expect = e-119, Method: Compositional matrix adjust. Identities = 230/541 (42%), Positives = 326/541 (60%), Gaps = 36/541 (6%) Query: 1 LSGCAFRSTGESLPAASPESTAPHFDVKTGKYNPPVDLYTVGSVNPNLTFKKGESLEHNV 60 L+GC+ + + + P ++A + PV L + V+ ++ F+ GE++E NV Sbjct: 17 LAGCS-EESNQKESSVEPVASANEL---PQTFEEPVTLNVIKHVSGDIFFRDGETIEDNV 72 Query: 61 HTIWAENRLGIRIRYLWTISGTSETYANKLRLEL-AKGNMPDVVTTRDANVIQELIDSGQ 119 HT W + + I + YLWT SG ET+ KL+L + A +P ++ R +++ Q+LIDSG+ Sbjct: 73 HTDWVKEQFNIDLNYLWTTSGPGETFDTKLQLSMSANEELPSILALR-SDITQDLIDSGR 131 Query: 120 FMEAGSLFGQFASKVWKQAVAEDPSAWDSYVRDGHKYAIPIMDYEYNSDPLLWIRQDWLD 179 ME G LF ++AS WK+A+A DP AWD Y+R+ + AIPI+DYE N D +L+IRQDW+D Sbjct: 132 VMEVGELFDKYASDTWKEAMAADPHAWDPYIREEGRMAIPILDYEMNGDSVLFIRQDWMD 191 Query: 180 KLHLSAPRTLDELEQVMDAFVNRDPDGNGLKDTYGLAVGFRNGPSTWMGDSSWIFGAFGT 239 L L P T+D++E++MDAFVN DP G G K TYGLAVGF N +TWM + W+FGA+G Sbjct: 192 ALGLEPPETMDDIEEIMDAFVNEDPTGTGEK-TYGLAVGFANALNTWMSEVDWVFGAYGG 250 Query: 240 VPEQWNRRSDGTLEYGSVQPGARKAVALMKQWVQQGYLSTDSAWLDEEGAANLFVSGKAG 299 +P+QWN DGTLE+GSVQP ++ + M++W+++GY+ +S DE AA LF SG+AG Sbjct: 251 MPDQWNLADDGTLEHGSVQPEIKEGLETMRRWMEKGYIHPESGLWDEGKAAELFTSGRAG 310 Query: 300 IIAGPYWMGGWPLSHLTSADPQARIKAAAIPAGPDGTVMRR-GTLPVNGAILINKKMMHP 358 IIAGP+WM WPLS L A +A IP+GPDG R G NGA++IN Sbjct: 311 IIAGPHWMPDWPLSELKQNVEGAEYQAYDIPSGPDGQKGRSTGISSHNGAVMINANATEE 370 Query: 359 EI--FFTYQNYLFDYYATST--GEFINGLAKDYDWTMDAGQPTIRPSALPLGGIRVASYT 414 EI FF YQNYLFD++A T EF G A+ YD+ ++ G+ +P G I YT Sbjct: 371 EIQAFFVYQNYLFDHFANPTEGSEFEYGFAEGYDYVIEDGEVKYSDDDIPGGRIDPVKYT 430 Query: 415 LTFDGARIPSKVIK------------------------EIPKDIAPVLLSQKEASRKEQF 450 LTFDGAR+PS IK E+ + A +++ ++A F Sbjct: 431 LTFDGARMPSLYIKTLADFARGQEPETPFQKKMYLQYPELAWEGARIVVDGEDAQIPSMF 490 Query: 451 TGPPTATMKSDGELLKKLEQKSFQKIIFADSGIGEFDEFVDKWKAYGGLTETSEVNAWDR 510 TG PT TM G+ L L +++F K+I+ + I +F+ VD+WKA GG T+EVN W + Sbjct: 491 TGAPTDTMLQKGDTLDSLLKENFSKMIYGEKPIDDFESLVDQWKASGGDDMTAEVNEWFK 550 Query: 511 S 511 S Sbjct: 551 S 551 >C3J6V6.1 C3J6V6_9BACI Extracellular solute-binding protein family 1 Length = 549 Score = 355 bits (910), Expect = 7e-96, Method: Compositional matrix adjust. Identities = 197/508 (38%), Positives = 286/508 (56%), Gaps = 28/508 (5%) Query: 33 NPPVDLYTVGSVNPNLTFKKGESLEHNVHTIWAENRLGIRIRYLWTISGTSETYANKLRL 92 N V + TV ++ + F++GE L +N T W+E LGI+ + LWT + E Y NK+RL Sbjct: 40 NGVVTITTVRTLKDDTKFREGEDLNNNPITRWSEKELGIKWKTLWT-APNDEQYHNKIRL 98 Query: 93 ELAKGN-MPDVVTTRDANVIQELIDSGQFMEAGSLFGQFASKVWKQAVAEDPSAWDSYVR 151 L+ G +PDV D +I +LI SG+ M ++AS K+ + P A+ Sbjct: 99 ALSSGQKLPDVFKVSDGQLINDLIRSGKVMPVDEAIEKYASPRLKKIYEQFPEAFYPATV 158 Query: 152 DGHKYAIPIMDYEYNSDPLLWIRQDWLDKLHLSAPRTLDELEQVMDAFVNRDPDGNGLKD 211 DG +Y IP SD LLWIR+DWLDKL L P+T+++LE++MDAFVN+DPDGNG KD Sbjct: 159 DGKRYGIPRFSGGNGSDSLLWIRKDWLDKLGLQPPKTIEDLEKIMDAFVNKDPDGNGKKD 218 Query: 212 TYGLAVGFRNGPSTWMGDSSWIFGAFGTV-PEQWNRRSDGTLEYGSVQPGARKAVALMKQ 270 T GL + +NG +TW+ D S+IFGA+G P W++ DG L YGSVQP +KA+A + + Sbjct: 219 TIGLTLASKNGLATWLADGSFIFGAYGNYEPGSWSKGEDGKLVYGSVQPSMKKALAKLNE 278 Query: 271 WVQQGYLSTDSAWLDEEGAANLFVSGKAGIIAGPYWMGGWPLSHLTSADPQARIKAAAIP 330 W Q+GYL + LDE+ A FV+GK+GII+ P W GWP++ +P A ++ +P Sbjct: 279 WYQKGYLDKEVGILDEQTAIKSFVAGKSGIISAPPWAAGWPIADALKNNPGAVVEPYPLP 338 Query: 331 AGPDGTVMRRGTLPVNGAILINKKMMHPEIFFTYQNYLFDYYATSTGEFINGLAKDYDWT 390 +GPDG + RRG V G L +K H + FF Y + + Y + F GLA+ YD+ Sbjct: 339 SGPDGKIGRRGEGLVTGMFLFSKDFKHMDKFFQYLDEAYAYVYNDSKYFKYGLAEGYDYV 398 Query: 391 MDAGQPTIRPSALPLGGIRVASYTLTFDGARIPSKV---------IKEIPKDI------- 434 M G+P +P G + +Y +T +P + K PK+ Sbjct: 399 MKNGKPVYDDQEIPGGRLDPGAYFITEAIPTVPYMLYDLVEELYTTKRQPKNAYEYTRIV 458 Query: 435 --------APVLLSQKEASRKE-QFTGPPTATMKSDGELLKKLEQKSFQKIIFADSGIGE 485 A +++Q+ R E +FTGPPT TM+ GE L K+E+++F II+ + + Sbjct: 459 SLGESFMKAATIVNQQNQYRIENEFTGPPTKTMQKRGEFLTKMERETFANIIYGRTPLSA 518 Query: 486 FDEFVDKWKAYGGLTETSEVNAWDRSRR 513 FD+FV KW+ GG T EVN W +S + Sbjct: 519 FDDFVKKWEESGGEEITKEVNEWYQSVK 546 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,536,868,437 Number of extensions: 162594392 Number of successful extensions: 378373 Number of sequences better than 1.0e-05: 199 Number of HSP's gapped: 378196 Number of HSP's successfully gapped: 206 Length of query: 513 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 373 Effective length of database: 1,741,754,103 Effective search space: 649674280419 Effective search space used: 649674280419 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)