BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0286.0002.p (638 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z9R5.1 C0Z9R5_BREBN Hypothetical membrane protein 375 e-102 B7K7J0.1 B7K7J0_CYAP7 Beta-lactamase 223 6e-56 Q8TR39.1 Q8TR39_METAC FmtA-like protein 205 1e-50 B1IEB1.1 B1IEB1_CLOBK Putative beta-lactamase 202 9e-50 C4IBX5.1 C4IBX5_CLOBU Beta-lactamase 201 2e-49 >C0Z9R5.1 C0Z9R5_BREBN Hypothetical membrane protein Length = 630 Score = 375 bits (964), Expect = e-102, Method: Compositional matrix adjust. Identities = 224/638 (35%), Positives = 355/638 (55%), Gaps = 17/638 (2%) Query: 5 MMKLKSVFIISVILWMGTFGIVPAAQAAGTDDAITQERLEETLTPLLNKIMEADHIPGTA 64 + K +V ++S ++ + +V A +T +E+ + P++ M HIPG A Sbjct: 6 LRKWFAVLLVSCLMLIQPLSVVNA------RSPLTALSIEQVVDPVMKNEMNNLHIPGAA 59 Query: 65 VVVTEGDRIVFSKGYGYADIGKQLPVDPGHTVMRVGSLTKSMTATAVMQLQEQGKVAMDQ 124 +V+T+GDRI+F KGYG+AD+ +Q+PVDP TV R+GS+TKS TATA MQL E+GK+++ + Sbjct: 60 IVITQGDRIIFKKGYGFADVEQQIPVDPERTVFRIGSVTKSFTATAAMQLVEKGKLSLRE 119 Query: 125 DINTYLPGFKVPLFHGHPIXXXXXXXXXAGLDEGVYNL-SAQSADKALSAETFLRQYLDT 183 D+NT L +K+ PI AG+DE +Y + +S S ET + + L Sbjct: 120 DLNTVLKDYKITNNRYGPITLHHLLTHTAGIDEKIYKRDNHESVIPEFSTET-VNETLQM 178 Query: 184 QPPVREPGTEYAYSNPGAGLAGFLVEQVTGSTLEAYMQHNLFGPLQMPSASLAHQESDPD 243 Q + PG+++ YSN G GL G ++EQ +G +L YM+ N+F PL M SA L E Sbjct: 179 QETIFTPGSKFQYSNAGTGLLGSVIEQASGQSLHDYMRENIFRPLGMDSAELYLPEDRSK 238 Query: 244 MAKSYQYQEGSFQEIPYSYPNLPGAGALSVIPDELAHYMIALLNEGSYQGQHILKQSTVE 303 +AKSY Y +Q IPYS PG+GA ++ P + A YMIA L+ GSY G +LK TV+ Sbjct: 239 LAKSYYYDGNGYQTIPYSGIIFPGSGAANMTPVDFAPYMIAHLSGGSYNGTSLLKPETVQ 298 Query: 304 EMQARQFTEHPDVEGVGYGLFRNRTDNGILTLSHTGDIDGFAAKMQLIPEHKLGILVVSN 363 M A+QF H + G+GYG F+ T NGI TL G IDGF +KM LIP +GI V N Sbjct: 299 SMHAQQFAAHKRMYGIGYGFFKGETKNGIPTLWANGGIDGFISKMVLIPSESIGIFVAVN 358 Query: 364 AESPGKPLRDQVTDAIV-RLLPEPLIQDPSVAPPSSPDLEQYVRTYTMGLGPQHGWGKWF 422 A PG L +V +A +LL E + + + + ++++ Y + L P++GWGK Sbjct: 359 ATHPGLELHGKVVNAFTDKLLTEQVRKPLTPVDGTIDEMKKLEGQYLLTLTPENGWGKGL 418 Query: 423 RWLGGKNFDVKNA-GETLRITGVFP-EGTGSKESKTYIPVREGLFRDKESGEFISFHQQE 480 R L + VK G+T+ +TG+F EG S E K ++ V + F++ + E + FH+++ Sbjct: 419 RLLSSYPYTVKALDGQTISVTGLFQNEGQQSLEKK-FVQVDDHFFQEIDGEEQLYFHEEQ 477 Query: 481 GVWKLTFIQGVTIEEKPPFLQQPSTLLAIYAGIGLAWPVIVLTVXXXXXXXXXXXXXXXX 540 G + +T ++ + ++ P LLA+Y G+ + ++ L Sbjct: 478 GKFYMTGPMNFSL-PRISIIESPPVLLALYVIPGIVFILLSLVWIARFGLRKWRKQSHAI 536 Query: 541 XGHAALIAVIFSIYLIGQLLYGNSQVLFGYPAWYAWGFSSLPXXXXXXXIHLGIKTVRQQ 600 ++A++ S++ I Q Y N+++++GYPA+Y + +SLP +L ++ + Sbjct: 537 SKVVPIMALLHSLFFIIQFTYANAELVYGYPAFYQFVVASLPIVSLAAAAYLLLR-IGTA 595 Query: 601 QNTDEKRMVRYGRYLLVVMSLGYTFFLFYWNMLSVHFS 638 + D ++V ++S+ YT +LFYWN LS+H+S Sbjct: 596 KERDRWKVVNSAA---AILSILYTGYLFYWNFLSIHYS 630 >B7K7J0.1 B7K7J0_CYAP7 Beta-lactamase Length = 669 Score = 223 bits (567), Expect = 6e-56, Method: Compositional matrix adjust. Identities = 141/447 (31%), Positives = 235/447 (52%), Gaps = 20/447 (4%) Query: 39 TQERLEETLTPLLNKIMEADHIPGTAVVVTEGDRIVFSKGYGYADIGKQLPVDPGHTVMR 98 QE +++ ++ + ME ++IPG V + + I+F+KGYGYA++ +++PV+P T+ R Sbjct: 58 NQEEVDKFFNTIIRRQMEQENIPGLVVSLVKDGEIIFNKGYGYANVEEKIPVEPDKTLFR 117 Query: 99 VGSLTKSMTATAVMQLQEQGKVAMDQDINTYLPGFKVPLFHGHPIXXXXXXXXXAGLDEG 158 V SL+K T TA+MQL E+GK+ + QD++ YL K+ + P+ G + Sbjct: 118 VASLSKLFTDTAIMQLYEEGKLNLYQDVSRYLDEVKIKNPYSVPVTAAELMTQTGGFPQA 177 Query: 159 VYNLSAQSADKALSAETFLRQYLDTQ-PPVR-EPGTEYAYSNPGAGLAGFLVEQVTGSTL 216 + L+A + ++ T L +YL Q PPV +PG Y+Y+N G L G+LV++++G Sbjct: 178 LIGLAAPTPEQM----TPLPEYLSEQLPPVTWQPGEIYSYANIGITLLGYLVQKISGIPF 233 Query: 217 EAYMQHNLFGPLQMPSASLAH---QESDPDMAKSYQYQEGSFQEIPYSYPNLPGAGALSV 273 Y+ N+F PLQM +S +E D+A YQY ++ +P+ Y N+ A A+S Sbjct: 234 VEYVDENIFKPLQMNRSSFRQPLPEELRSDLAVGYQYNGNEYKPVPFLYLNIAPAAAMST 293 Query: 274 IPDELAHYMIALLNEGSYQGQHILKQSTVEEMQARQFTEHPDVEGVGYGLFRNRTDNGIL 333 ++AH+MIA L G Y+ Q ILK T M + FT HP + G YG F R +N I Sbjct: 294 TGTDMAHFMIAHLQLGKYKNQRILKPDTALLMHRQHFTHHPLLPGTAYG-FHERLENNIR 352 Query: 334 TLSHTGDIDGFAAKMQLIPEHKLGILVVSNA-ESPGKPLRDQVTDAIVRLLPEPLIQDPS 392 ++ H G++ G+ + + L+P+ LG+ + SN+ + + Q D + E L Q P Sbjct: 353 SIGHYGNLRGYTSSITLLPDQNLGLFIASNSFNGINENIITQFFDHFFK--AENLPQAPQ 410 Query: 393 VAPPSSPDLEQYVRTYTMGLGPQHGWGKWFRWLGGKNFDVKNAGETLRIT---GVFPEGT 449 + LE+YV TY P++ K G + + ++ TL I FP+ Sbjct: 411 PEQQLNISLEKYVGTYRDLEYPRYSIAKLTAVAGHLHIE-RSEDNTLTINPPGWFFPKDF 469 Query: 450 GSKESKTYIPVREGLFRDKESGEFISF 476 + +PV LF+ ++ +++F Sbjct: 470 ---KPIKLLPVDNQLFQRADNTGYVAF 493 >Q8TR39.1 Q8TR39_METAC FmtA-like protein Length = 671 Score = 205 bits (522), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 118/337 (35%), Positives = 177/337 (52%), Gaps = 26/337 (7%) Query: 43 LEETLTPLLNKIMEADHIPGTAVVVTEGDRIVFSKGYGYADIGKQLPVDPGHTVMRVGSL 102 LE + +N ++A+HIPG V V + +I+F+KGYGYADI K+ PV T+ RVGS+ Sbjct: 69 LEAFMDGAVNAQLKANHIPGATVAVVKDGQIIFAKGYGYADIDKRQPVVGNQTLFRVGSV 128 Query: 103 TKSMTATAVMQLQEQGKVAMDQDINTYLPGFKVPLFHGHPIXXXXXXXXXAGLDEGVYNL 162 +K TAVMQL EQGK+ +D D+NTYL F+VP + PI +G + Sbjct: 129 SKLFVWTAVMQLAEQGKLDLDADVNTYLKDFQVPATYSKPITLKNLMSHTSGFE------ 182 Query: 163 SAQSADKALSAETFLRQYLDTQP-----------PVREPGTEYAYSNPGAGLAGFLVEQV 211 D A+S F+ D P VR PG AYSN G+ LA ++VEQV Sbjct: 183 -----DLAISGRFFVHNSTDIMPLEEYLKKGMPARVRPPGELTAYSNYGSALAAYIVEQV 237 Query: 212 TGSTLEAYMQHNLFGPLQMPSASLAH---QESDPDMAKSYQYQEGSFQEIPYSYPNLPGA 268 +G + Y++ N+ PL M + + +M+ Y Y ++ P+ Y + A Sbjct: 238 SGMPFDRYVEENILLPLDMNNTTFDQPLPARLASNMSNGYVYSNNAYTAKPFEYVQVWPA 297 Query: 269 GALSVIPDELAHYMIALLNEGSYQGQHILKQSTVEEMQARQFTEHPDVEGVGYGLFRNRT 328 ++S +++A +MIA L G Y + IL+++T ++M +R FT P V GV YG Sbjct: 298 SSMSSTSEDMAKFMIAHLQNGRYGDKRILQEATAQQMHSRLFTNAPGVNGVTYGFLEINP 357 Query: 329 DNGILTLSHTGDIDGFAAKMQLIPEHKLGILVVSNAE 365 +N + + H GD F ++ LIPE +LG+ + N E Sbjct: 358 ENPRV-IGHEGDTILFHTQLALIPESQLGLFISLNEE 393 >B1IEB1.1 B1IEB1_CLOBK Putative beta-lactamase Length = 653 Score = 202 bits (514), Expect = 9e-50, Method: Compositional matrix adjust. Identities = 142/490 (28%), Positives = 239/490 (48%), Gaps = 33/490 (6%) Query: 6 MKLKSVFIISVIL--WMGTFGIVPAAQAAGTDDAI----------TQERLEETLTPLLNK 53 MK+K+ I+++L T G+ + A D+ E L++ + K Sbjct: 1 MKIKAKRFIAILLSTIFITLGLSSNSLAKEIDNTKELNKKHVATNNSEDLQQFMDKFFKK 60 Query: 54 IMEADHIPGTAVVVTEGDRIVFSKGYGYADIGKQLPVDPGHTVMRVGSLTKSMTATAVMQ 113 ME IPG A+VV + + VF KGYGY+D+ +++ VDP TV S++K TATA+MQ Sbjct: 61 NMEKYSIPGAAIVVVKDGKEVFKKGYGYSDVKEKILVDPDKTVFPAASVSKLFTATAIMQ 120 Query: 114 LQEQGKVAMDQDINTYLPGFKVPLFHGHPIXXXXXXXXXAGLDEGVYNLSAQSADKALSA 173 LQE+GK+ ++++I+ Y+ +K+ + P+ +G+DE +KA+ + Sbjct: 121 LQEEGKIDLNENIDKYIKPYKIINNYKEPVTCRNLLTHSSGVDEASELNGGTKDEKAIKS 180 Query: 174 ETFLRQYLDTQPP--VREPGTEYAYSNPGAGLAGFLVEQVTGSTLEAYMQHNLFGPLQMP 231 + + Y D P VREP T Y N G L G++VE+V+G + E Y+ N+ PL+M Sbjct: 181 QEY---YFDNHIPRVVREPNTASRYCNQGYNLLGYIVEKVSGISYEEYINKNILEPLKME 237 Query: 232 SASLAHQESDPDMAKSYQYQEGSFQEIPYSYPNLPGAGALSVIPDELAHYMIALLNEGSY 291 ++ + + ++ + Y +G + E P +Y G+ ++ ++ ++MIA LN G + Sbjct: 238 NSLVRLKNNNTTKSYGYGGMDGIYNESPLAYQYTSGSSGINATVKDMENFMIAHLNNGVF 297 Query: 292 QGQHILKQSTVEEMQARQFTEHPDVEGVGYGLFR-NRTDNGILTLSHTGDI-DGFAAKMQ 349 Q IL + T M+ +QF+ + G+GYG R NR NG L H G + G + Sbjct: 298 QENRILNEKTSIMMKNKQFSNDNSLPGMGYGFIRSNR--NGEEILKHEGGLPSGSTTTLF 355 Query: 350 LIPEHKLGILVVSNAESPGKPLRDQVTDAIVRLLPEPLIQDPSVAPPSSPDLEQYVRTYT 409 L+P+ KLGI V +N+ +P P + + + P + P D +Y TY Sbjct: 356 LMPKEKLGIYVATNSLNP-LPFNFE-EECLNYFYPSKSNNFNKIKPNPFKDFSKYEGTYR 413 Query: 410 MGLGPQHGWGKWFRWLGGKNFDVK---NAGETLRITGVFPEGTGSKE--SKTYIPVREGL 464 G +L + D++ N TL + E T +KE + T + + G+ Sbjct: 414 SYDGIAKSNIMKIGFLFDPSMDMRITDNKDGTLNL----KECTSAKEEVTTTLVEKKNGV 469 Query: 465 FRDKE-SGEF 473 F K+ G+F Sbjct: 470 FARKDGRGDF 479 >C4IBX5.1 C4IBX5_CLOBU Beta-lactamase Length = 658 Score = 201 bits (511), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 119/374 (31%), Positives = 197/374 (52%), Gaps = 20/374 (5%) Query: 6 MKLKSVFIISVILWMGT-----FGIVPAAQAAGTD----DAITQERLEETLTPLLNKIME 56 +KLKSV I +I+ M F I A +D D + ++ + N+ ME Sbjct: 12 IKLKSVVRIFLIILMSIGLISGFTINAAMSKEKSDEVHGDIENNDDVKNFIDNYFNENME 71 Query: 57 ADHIPGTAVVVTEGDRIVFSKGYGYADIGKQLPVDPGHTVMRVGSLTKSMTATAVMQLQE 116 +PG +VVV + ++ F GYGY+++ + V+P T+ GS++K TATA++QL E Sbjct: 72 KYSVPGASVVVVKHNKEAFKMGYGYSNLESKSLVNPDETIFPAGSVSKLFTATAILQLYE 131 Query: 117 QGKVAMDQDINTYLPGFKVPLFHGHPIXXXXXXXXXAGLD-EGVYNLSAQSADKALSAET 175 +GK+ +D ++N Y+ +K+ + P+ +GLD E NLS A+ S E Sbjct: 132 EGKIDLDTNVNDYINPYKISNMYNEPVTCRNLLTHSSGLDEESELNLSTTDANSIKSQE- 190 Query: 176 FLRQYLDTQP--PVREPGTEYAYSNPGAGLAGFLVEQVTGSTLEAYMQHNLFGPLQMPSA 233 Y + P + EP T YSN G L G++VE+V+G + E Y++ + PL M ++ Sbjct: 191 ---YYFNNHPLKVITEPNTICRYSNIGYNLLGYIVEKVSGISYEEYVEEKILKPLNMSNS 247 Query: 234 SLAHQESDPDMAKSYQYQEGSFQEIPYSYPNLPGAGALSVIPDELAHYMIALLNEGSYQG 293 + + S D+AK Y Y + + E+P+SY G+ + ++ +++IA LN+G +Q Sbjct: 248 LVRLKNS--DIAKGYMYNDEKYSELPFSYQYTSGSSGIITTVKDMENFIIANLNDGKFQN 305 Query: 294 QHILKQSTVEEMQARQFTEHPDVEGVGYGLFRNRTDNGILTLSHTGDI-DGFAAKMQLIP 352 ILK T+ M +QF+ +EG+G+G R+ NG + H G + G+ + LIP Sbjct: 306 NSILKPKTLSLMHEKQFSNSDALEGMGFGFIRSYR-NGQEIIKHEGGLPSGYTTTLFLIP 364 Query: 353 EHKLGILVVSNAES 366 + LGI V +N+ S Sbjct: 365 KENLGIYVATNSLS 378 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,777,409,026 Number of extensions: 160539967 Number of successful extensions: 370771 Number of sequences better than 1.0e-05: 2366 Number of HSP's gapped: 368104 Number of HSP's successfully gapped: 2408 Length of query: 638 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 496 Effective length of database: 1,722,912,073 Effective search space: 854564388208 Effective search space used: 854564388208 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)