BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0284.0020.p (816 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A0P8W9.1 A0P8W9_BACCI Isocyclomaltooligosaccharide glucanotransf... 228 2e-57 A0P8X0.1 A0P8X0_BACCI Alpha-amylase 227 3e-57 Q08T95.1 Q08T95_STIAU Beta/alpha-amylase EC=3.2.1.1 EC=3.2.1.2 181 3e-43 A7AK94.1 A7AK94_9PORP Putative uncharacterized protein 156 7e-36 Q08SE2.1 Q08SE2_STIAU Alpha amylase, catalytic domain subfamily,... 154 3e-35 >A0P8W9.1 A0P8W9_BACCI Isocyclomaltooligosaccharide glucanotransferase Length = 995 Score = 228 bits (581), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 116/198 (58%), Positives = 142/198 (71%), Gaps = 2/198 (1%) Query: 621 NTATIYYRK-GFATPYIHYQPDGEK-WTTAPGVAMADSEYNGYSKSTITLGSATGLTAAF 678 NTATIYY+ F+ YIHY+ DG WTT+PGV M S ++GY TI LG+ATGLTAAF Sbjct: 798 NTATIYYKNTAFSNSYIHYKLDGATTWTTSPGVPMQASTFSGYKSITIPLGTATGLTAAF 857 Query: 679 NNGSGMWDNNGGANYKFLAGVSTFINGKITSGFPHPDGVTIVISVPANTPANEDLYLTSN 738 NNGSG WD+NGG NY F G S+ + G +T+G P D VT +SVP +TPAN +YLT + Sbjct: 858 NNGSGTWDSNGGNNYHFGTGSSSLVGGSLTTGEPQADSVTFRVSVPGSTPANAPVYLTGS 917 Query: 739 LTGWNTADANYKLTRNADGTYSIKLNVAAGTNIQYKITRGSWATVEVNSNGSDIANRSLT 798 WN AD Y LTR +DG YS+ LN+ AGT + YK+TRGSWATVE S+G+DI NR+LT Sbjct: 918 FNSWNAADTAYLLTRGSDGVYSVTLNLPAGTAVTYKLTRGSWATVETTSSGADITNRTLT 977 Query: 799 TTAGAPTVSISVQRWKDK 816 GA TV+ISVQRWKD+ Sbjct: 978 PAGGAQTVTISVQRWKDQ 995 Score = 174 bits (442), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 92/207 (44%), Positives = 131/207 (63%), Gaps = 4/207 (1%) Query: 510 QQIT---GTDVDTLWNEYQVSLDKSQPTAPGDLXXXXXXXXXXXLSWSASTDNLGVAGYN 566 +QIT G + ++ + Q D P+ PG++ +SWSASTDN+GV GY Sbjct: 536 KQITVTLGANSAKIYAKTQPVTDTQAPSVPGNVTATVQNASSALVSWSASTDNVGVTGYE 595 Query: 567 IYSNGIKISTTQSTNYKVNGLTTGSSYTFMVKAVDHSGNESSASNTITVIAKASNTATIY 626 IY NG+KI T+ +T++ NGL ++Y++ VKA D + N S+ S ++ A N+ TIY Sbjct: 596 IYRNGVKIGTSATTSFTDNGLVGSTNYSYTVKAYDAAMNLSAFSAAALIVTPAGNSVTIY 655 Query: 627 YRKGFATPYIHYQPDGEKWTTAPGVAMADSEYNGYSKSTITLGSATGLTAAFNNGSGMWD 686 Y++G+ PYIHY+P G WTT+PGVA+ +E GY+K TI +G+AT L A FNNGSG+WD Sbjct: 656 YKQGYTNPYIHYRPVGGTWTTSPGVAIPAAEVAGYNKITINIGAATQLEACFNNGSGIWD 715 Query: 687 NNGGANYKFLAGVSTFI-NGKITSGFP 712 +NGG+NY F G T+ G I +G P Sbjct: 716 SNGGSNYLFGTGTWTYTPTGNIQAGGP 742 >A0P8X0.1 A0P8X0_BACCI Alpha-amylase Length = 1290 Score = 227 bits (579), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 116/212 (54%), Positives = 147/212 (69%), Gaps = 2/212 (0%) Query: 607 SSASNTITVIAKASNTATIYYRK-GFATPYIHYQPDGE-KWTTAPGVAMADSEYNGYSKS 664 S ++ + A N+ATIYY+ F+ YIHY+ DG WTT+PGV M S ++GY Sbjct: 1079 SPSATPVPTATPAGNSATIYYKNTAFSNSYIHYKLDGATAWTTSPGVQMQASTFSGYKAI 1138 Query: 665 TITLGSATGLTAAFNNGSGMWDNNGGANYKFLAGVSTFINGKITSGFPHPDGVTIVISVP 724 TI LGSATGLTAAFNNGSG+WDNNGG+NY F G S+ G + +G P D VT +SVP Sbjct: 1139 TIPLGSATGLTAAFNNGSGIWDNNGGSNYHFGTGSSSLTGGNLITGEPQADSVTFRVSVP 1198 Query: 725 ANTPANEDLYLTSNLTGWNTADANYKLTRNADGTYSIKLNVAAGTNIQYKITRGSWATVE 784 +TPAN +YLT + WN AD Y LTR +DG YSI LN+ AG+ + YK+TRGSWATVE Sbjct: 1199 GSTPANAPVYLTGSFNSWNAADPAYLLTRGSDGIYSITLNLPAGSAVTYKLTRGSWATVE 1258 Query: 785 VNSNGSDIANRSLTTTAGAPTVSISVQRWKDK 816 S+G+DI NR+LT GA TV+++VQRWKD+ Sbjct: 1259 TASSGADITNRTLTPAGGAQTVTLTVQRWKDQ 1290 Score = 172 bits (437), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 95/201 (47%), Positives = 124/201 (61%), Gaps = 2/201 (0%) Query: 529 DKSQPTAPGDLXXXXXXXXXXXLSWSASTDNLGVAGYNIYSNGIKISTTQSTNYKVNGLT 588 D P P +L LSW+A+TDN+ V GY IY NG++I TT ST+Y +GL+ Sbjct: 811 DTEAPAVPANLQATVMNASSVGLSWNAATDNIRVTGYEIYRNGVRIGTTPSTSYTDSGLS 870 Query: 589 TGSSYTFMVKAVDHSGNESSASNTITVIAKASNTATIYYRKGFATPYIHYQPDGEKWTTA 648 ++Y + VKA D SGN S S T A N T+YY++G++TPYIHY+P G WTTA Sbjct: 871 ASTAYEYRVKAYDASGNLSGFSAAATATTPAGNHVTVYYKQGYSTPYIHYRPAGGTWTTA 930 Query: 649 PGVAMADSEYNGYSKSTITLGSATGLTAAFNNGSGMWDNNGGANYKFLAGVSTFI-NGKI 707 PGVA+ +E GY+K TI +G+AT L A FNNGSG WD+NGG+NY F G T+ GKI Sbjct: 931 PGVAIPAAEVAGYNKITINIGAATQLEACFNNGSGTWDSNGGSNYLFGTGTWTYTPTGKI 990 Query: 708 TSGFPHPDGVTIVISVPANTP 728 +G P T ++ P TP Sbjct: 991 QAGAPVAPSATPTVA-PTATP 1010 >Q08T95.1 Q08T95_STIAU Beta/alpha-amylase EC=3.2.1.1 EC=3.2.1.2 Length = 1451 Score = 181 bits (459), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 96/198 (48%), Positives = 125/198 (63%), Gaps = 2/198 (1%) Query: 529 DKSQPTAPGDLXXXXXXXXXXXLSWSASTDNLGVAGYNIYSNGIKISTTQSTNYKVNGLT 588 D + P+ P L LSW+ASTDN GVAGY +Y NG +I+ T Y + L+ Sbjct: 892 DATAPSVPTGLSASAVTSSSVTLSWTASTDNYGVAGYFVYRNGTQIAAPTGTGYTDSSLS 951 Query: 589 TGSSYTFMVKAVDHSGNESSASNTITVIAKASNTATIYYRKGFATPYIHYQPDGEKWTTA 648 + Y++ VKA D +GN S+AS +++ NTAT+YY+KGFATPYIHY+P G WTT Sbjct: 952 PSTVYSYTVKAADAAGNTSAASAALSITTSTGNTATVYYKKGFATPYIHYRPAGGTWTTP 1011 Query: 649 PGVAMADSEYNGYSKSTITLGSATGLTAAFNNGSGMWDNNGGANYKFLAGVSTFINGKIT 708 PG+AM D+E G++K T+ L +AT L A FNNGSG WD+N G NY F G+STF G IT Sbjct: 1012 PGIAMPDAEVPGHAKYTVNLATATQLEAVFNNGSGTWDSNNGNNYFFPTGISTFNAGVIT 1071 Query: 709 SGFPHPDGVTIVISVPAN 726 G P D T+ S P+N Sbjct: 1072 PGGPVVD--TVSPSAPSN 1087 Score = 181 bits (458), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 93/188 (49%), Positives = 123/188 (65%) Query: 528 LDKSQPTAPGDLXXXXXXXXXXXLSWSASTDNLGVAGYNIYSNGIKISTTQSTNYKVNGL 587 +D P+AP +L LSW+ASTD++GV GY ++ ++ T+ T Y +GL Sbjct: 1077 VDTVSPSAPSNLTSPSKTVSSVTLSWTASTDDVGVTGYLVFRGSTQVGTSTDTTYTDSGL 1136 Query: 588 TTGSSYTFMVKAVDHSGNESSASNTITVIAKASNTATIYYRKGFATPYIHYQPDGEKWTT 647 ++Y++ VKA D +GN S+AS ++V + NT T+YY+KGFATPYIHY+P G WTT Sbjct: 1137 AANTAYSYTVKARDAAGNTSAASTALSVTTASGNTVTVYYKKGFATPYIHYRPAGGTWTT 1196 Query: 648 APGVAMADSEYNGYSKSTITLGSATGLTAAFNNGSGMWDNNGGANYKFLAGVSTFINGKI 707 APGVAM ++E GY+KST+ LGSAT L FNNGSG WDNNGG NY AG TF G + Sbjct: 1197 APGVAMPNAEVTGYAKSTLNLGSATQLECVFNNGSGTWDNNGGLNYFIPAGTHTFNAGTV 1256 Query: 708 TSGFPHPD 715 T+G P D Sbjct: 1257 TAGAPSGD 1264 >A7AK94.1 A7AK94_9PORP Putative uncharacterized protein Length = 332 Score = 156 bits (395), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 105/327 (32%), Positives = 159/327 (48%), Gaps = 21/327 (6%) Query: 220 FDFGQQIVIDGYAFSAANSAPDSDPKIWDLKASTDNVNWITIDSRNSEDF--RYRHQRNH 277 FD + I Y ++ P DP W LK S D NW+ +D R + F RY+ Sbjct: 11 FDEQYTVPIQSYKIYSSGETPVHDPSAWTLKGSYDGKNWVVVDERKDQTFCSRYQEILCS 70 Query: 278 YVLPTNQKAYRYYRFELYNHSGDTLQIGDVAF--SRPDDAWHTVA-PIIDMHNLD--TTN 332 P+N Y+ Y E GDTL +GDV + W P ID +D T Sbjct: 71 ITKPSN---YKQYMLEAATAVGDTLVLGDVVLFDENLNAGWEDFKYPEIDYEVIDPETKG 127 Query: 333 GYLFDQAIPDPQSDILAVIRQVCNTLYASPADVPIRPRTLHVTIGNYDGVASVSGGPTDA 392 ++ + +P I R+V L+ S D + +H T+ +YDGV++ SG P + Sbjct: 128 AAIYADLVQNPDEYIRYHARKVAEILFYSAKDTMNDVQKVHYTLKDYDGVSAKSGNPANT 187 Query: 393 DLTISSRYL-KSYADSGKPLRQEILGILYHELTHVYQFDDRGT------PDIGYMIEGMA 445 + S++++ KS +S L E G+L+HEL H YQF+ +G IEG+A Sbjct: 188 SIVYSTQHIEKSANESLYKLDFETRGVLFHELVHAYQFEPKGIGSYSTNKTFWACIEGLA 247 Query: 446 DAVRFENGYHDRYSMTPGGTWHDGYGTSGNFFRWIDEKKHTGFLRELNASLNPFDGQTWT 505 DAVR + GY D + PGG W DGY T+G F +W+ K +R+ + ++ D ++ Sbjct: 248 DAVRAQAGYFDMSTRKPGGNWMDGYRTTGFFIQWLTTKDPDA-IRKFHETVRDLDEWSFD 306 Query: 506 PAVFQQITGTD--VDTLWNEYQVSLDK 530 A+ +++ G D ++ LWNEYQ L K Sbjct: 307 KAM-KRMFGDDASIEGLWNEYQAFLSK 332 Score = 64.3 bits (155), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 33/91 (36%), Positives = 46/91 (50%) Query: 82 FPGQSTYALNSYKITSANDHPERDPQGWTLEASNDGVIWTAVDSRQNENFSYRFQSKSYS 141 F Q T + SYKI S+ + P DP WTL+ S DG W VD R+++ F R+Q S Sbjct: 11 FDEQYTVPIQSYKIYSSGETPVHDPSAWTLKGSYDGKNWVVVDERKDQTFCSRYQEILCS 70 Query: 142 FSNTTAYKYYRFNFQCLSGTIVQLSEIELFD 172 + + YK Y G + L ++ LFD Sbjct: 71 ITKPSNYKQYMLEAATAVGDTLVLGDVVLFD 101 >Q08SE2.1 Q08SE2_STIAU Alpha amylase, catalytic domain subfamily, putative (Fragment) Length = 1055 Score = 154 bits (390), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 90/209 (43%), Positives = 123/209 (58%), Gaps = 11/209 (5%) Query: 526 VSLDKSQPTAPGDLXXXXXXXXXXXLSWSASTDNLGVAGYNIYSNGIKISTTQSTNYKVN 585 S D P+ P L LSW+ASTDN+GV GY ++ NG ++ T+ T Y Sbjct: 121 CSPDTQAPSTPAGLTKGTVTASSVTLSWTASTDNVGVTGYTLHRNGTQVGTSAGTTYTDT 180 Query: 586 GLTTGSSYTFMVKAVDHSGNESSASN--TITVIAKASNTATIYY---RKGFATPYIHYQP 640 GLT G+SY++ VKA+D +GN SSAS TIT + +N A IYY +G+++ +HY P Sbjct: 181 GLTAGTSYSYTVKALDAAGNVSSASAALTITTASVGTNQAEIYYYTKTRGWSSVNLHYAP 240 Query: 641 DGEKWTTAPGVAMADSEYNGYSKSTITLGSATGLTAAFNNGSGMWDNNGGANYKFLAGVS 700 G WTT PGVAM + + K T++LGSATGL A FNNGS +WDNNG ANY G+ Sbjct: 241 TGGAWTTPPGVAMNEQACTDWVKKTVSLGSATGLAATFNNGS-IWDNNGNANYALGTGLI 299 Query: 701 TFINGKITSGFPHPDGVTIVISVPANTPA 729 T +G +T+ P +++P +T A Sbjct: 300 TVKDGAVTAQATSP-----CVTLPPDTTA 323 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.131 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 5,344,867,496 Number of extensions: 244097366 Number of successful extensions: 431322 Number of sequences better than 1.0e-05: 525 Number of HSP's gapped: 431131 Number of HSP's successfully gapped: 754 Length of query: 816 Length of database: 3,060,696,203 Length adjustment: 144 Effective length of query: 672 Effective length of database: 1,704,070,043 Effective search space: 1145135068896 Effective search space used: 1145135068896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 136 (57.0 bits)