BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0284.0005.p (324 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAH3.1 B3KAH3_9BACI Binding-protein-dependent transport system... 471 e-131 B1DB00.1 B1DB00_9BACL Binding-protein-dependent transport system... 392 e-107 B1D598.1 B1D598_9BACL Binding-protein-dependent transport system... 380 e-103 C5C4L6.1 C5C4L6_9MICO Binding-protein-dependent transport system... 339 2e-91 Q5WL27.1 Q5WL27_BACSK Lactose ABC transporter permease 338 3e-91 >B3KAH3.1 B3KAH3_9BACI Binding-protein-dependent transport systems inner membrane component Length = 317 Score = 471 bits (1211), Expect = e-131, Method: Compositional matrix adjust. Identities = 226/300 (75%), Positives = 266/300 (88%) Query: 25 FRSRWNAPLAGYLFISPWLIGFLALTAYPLFLSLYYSFTDYTLLQPMKWIGTRNYERIFT 84 FR RW++PLAGYLFISPWLIGFLALTAYP+ LSLYYSFT+YTL++P++W+G NYERI T Sbjct: 18 FRKRWDSPLAGYLFISPWLIGFLALTAYPMLLSLYYSFTNYTLMKPIEWVGLDNYERIIT 77 Query: 85 ADPKFIQSAKVTFMYVLASVPLKLIAALFVAIILSRAVKGISFYRTAIYFPSLIGGSIGV 144 AD KF QS K+TF YVLASVPLKLIAAL VA++L +AV+GIS YRTAIYFPSLIG SI V Sbjct: 78 ADDKFAQSLKLTFYYVLASVPLKLIAALMVALLLKKAVRGISVYRTAIYFPSLIGASIAV 137 Query: 145 SLLWRNIFGVDGIFNKLIGVFGFEGKGWITSPDTALSTLILLTVWQFGSTMVIFLAGLKQ 204 SLLW+NIFGVDG FN+++G FG G GW+T+PDTAL +LI L+VWQFGS+MVIFLAGLKQ Sbjct: 138 SLLWKNIFGVDGFFNQVLGFFGIPGTGWVTNPDTALWSLIGLSVWQFGSSMVIFLAGLKQ 197 Query: 205 IPNDLYEASSVDGANKITQFFRITLPMLSPILYFNLIMAVIGAFQMFTSAFVITNGGPMN 264 IPNDLYEAS+VDGA K+ QFF+ITLPMLSP L+FNLIM VI +FQMFT A+VIT GGPMN Sbjct: 198 IPNDLYEASAVDGAGKLKQFFKITLPMLSPTLFFNLIMGVINSFQMFTPAYVITGGGPMN 257 Query: 265 STYVYAMYLYERAFSRYELGYASALAWIMLVAIIAATVLISYTSKYWVFYETDTGGKKRK 324 STYVYA+YLYERAFSRY+LGY+S+LAWIML+ I+AA V+I+ TSKYWVFYE+++ G K K Sbjct: 258 STYVYALYLYERAFSRYQLGYSSSLAWIMLILIVAAAVIINLTSKYWVFYESESQGGKGK 317 >B1DB00.1 B1DB00_9BACL Binding-protein-dependent transport systems inner membrane component Length = 318 Score = 392 bits (1008), Expect = e-107, Method: Compositional matrix adjust. Identities = 191/323 (59%), Positives = 248/323 (76%), Gaps = 5/323 (1%) Query: 1 MNXXXXRPPAAIAQSAAKTKKSSYFRSRWNAPLAGYLFISPWLIGFLALTAYPLFLSLYY 60 M PAA A K + + + + LAGY+FISPWLIGFL LTA+P+ S Y Sbjct: 1 MKAEAMNKPAA----ATKLRLTKKLKGTNDNNLAGYIFISPWLIGFLLLTAWPIIQSFYL 56 Query: 61 SFTDYTLLQPMKWIGTRNYERIFTADPKFIQSAKVTFMYVLASVPLKLIAALFVAIILSR 120 SFT+Y+LL+ W GT NY IFT+D F S KVTF++VL SVPLKL +L VA++L++ Sbjct: 57 SFTEYSLLEAPTWTGTDNYANIFTSDNDFKTSLKVTFIFVLFSVPLKLFFSLMVAMMLNK 116 Query: 121 AVKGISFYRTAIYFPSLIGGSIGVSLLWRNIFGVDGIFNKLIGVFGFEGKGWITSPDTAL 180 VKG++ YRTAIYFPSLIGGSI V+ LWRN+F ++G FN ++ FG +G GWIT+P T+L Sbjct: 117 NVKGMNLYRTAIYFPSLIGGSIAVAALWRNMFNLNGYFNHVLSWFGIQGVGWITNPHTSL 176 Query: 181 STLILLTVWQFGSTMVIFLAGLKQIPNDLYEASSVDGANKITQFFRITLPMLSPILYFNL 240 TLILL WQFGSTMVIFLAGLKQIP +LYE++SVDGA+K+ +FF ITLPMLSP+++FNL Sbjct: 177 GTLILLNTWQFGSTMVIFLAGLKQIPGELYESASVDGASKLRKFFHITLPMLSPVMFFNL 236 Query: 241 IMAVIGAFQMFTSAFVITNGGPMNSTYVYAMYLYERAFSRYELGYASALAWIMLVAIIAA 300 ++ +IG+FQMFTSAF++T GGP+NSTY+YA++LYE+AF Y++GYASALAWI+LV + Sbjct: 237 VLGIIGSFQMFTSAFIVTQGGPINSTYMYALFLYEKAFKHYQMGYASALAWILLVIVALM 296 Query: 301 TVLISYTSKYWVFYETDTGGKKR 323 T + S+YWVFYETD GGK++ Sbjct: 297 TAINFLVSRYWVFYETD-GGKQK 318 >B1D598.1 B1D598_9BACL Binding-protein-dependent transport systems inner membrane component Length = 316 Score = 380 bits (975), Expect = e-103, Method: Compositional matrix adjust. Identities = 190/315 (60%), Positives = 238/315 (75%), Gaps = 4/315 (1%) Query: 10 AAIAQSAAKTKKSSYFRS-RWNAPLAGYLFISPWLIGFLALTAYPLFLSLYYSFTDYTLL 68 AA+ AA+ ++ RS RW P+ GYLF+SPWLIGFL LT P+ +SLYYSFTDY+LL Sbjct: 4 AAVNSGAAQLEQVKKIRSKRWETPITGYLFLSPWLIGFLLLTLGPMIMSLYYSFTDYSLL 63 Query: 69 QPMKWIGTRNYERIFTADPKFIQSAKVTFMYVLASVPLKLIAALFVAIILSRAVKGISFY 128 + W+G NY++IFT D F +S K+T +VL +VPL+L+ AL +A++L+R +KG+SFY Sbjct: 64 EAPNWVGFDNYKQIFTNDDVFPKSLKITITFVLFTVPLRLLFALCIAMLLNRKMKGMSFY 123 Query: 129 RTAIYFPSLIGGSIGVSLLWRNIFGVDGIFNKLIGVFGFEGKGWITSPDTALSTLILLTV 188 RT +YFPSLIG SI VS+LW+NIF +G N + +FG +G WI P AL TLI+L V Sbjct: 124 RTMVYFPSLIGTSIAVSILWKNIFSRNGFINDFLAIFGIKGPAWIADPKYALGTLIILMV 183 Query: 189 WQFGSTMVIFLAGLKQIPNDLYEASSVDGANKITQFFRITLPMLSPILYFNLIMAVIGAF 248 WQFGS+M+IFLAGLKQIP DLYEASSVDGA K QF +TLPMLSPI+ FN + +I F Sbjct: 184 WQFGSSMIIFLAGLKQIPTDLYEASSVDGAGKFRQFISVTLPMLSPIILFNAVQTMINTF 243 Query: 249 QMFTSAFVITNGGPMNSTYVYAMYLYERAFSRYELGYASALAWIMLVAI-IAATVLISYT 307 QMFT AFVITNGGP+ STYVY MYLYE AF+++ +GYASALAWI+L+ I + A VL T Sbjct: 244 QMFTQAFVITNGGPVKSTYVYVMYLYEEAFTKFHMGYASALAWILLILIGLFAGVLFG-T 302 Query: 308 SKYWVFYETDTGGKK 322 SKYWVFYE + GGKK Sbjct: 303 SKYWVFYE-NQGGKK 316 >C5C4L6.1 C5C4L6_9MICO Binding-protein-dependent transport systems inner membrane component Length = 324 Score = 339 bits (869), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 162/318 (50%), Positives = 232/318 (72%), Gaps = 6/318 (1%) Query: 7 RPPAAIAQSAAKTKKSSYFRSRWNAPLAGYLFISPWLIGFLALTAYPLFLSLYYSFTDYT 66 RP AA+ T RSR A L Y F+ PWLIG + LT P+ LSLYYSF++Y+ Sbjct: 11 RPTAAVVVPRNVTPP---LRSRLRASLPAYGFLLPWLIGLVVLTIGPMALSLYYSFSEYS 67 Query: 67 LLQPMKWIGTRNYERIFTADPKFIQSAKVTFMYVLASVPLKLIAALFVAIILSRAVKGIS 126 LL P +WIG NY +F+ DP+++ SA+VT YVL SVPL+++ AL VA++L+R ++GI+ Sbjct: 68 LLAPPEWIGLDNYREMFS-DPRYLASARVTLTYVLVSVPLEMVIALGVAMLLNRGLRGIA 126 Query: 127 FYRTAIYFPSLIGGSIGVSLLWRNIFGVDGIFNKLIGVFGFEGKGWITSPDTALSTLILL 186 YR Y PSL+GGS+ +++LWR IFG +G+ N ++G FG E W+T+PDT+L TLI+L Sbjct: 127 AYRAVYYVPSLLGGSVAIAILWREIFGSEGLVNDVLGWFGIESGSWVTNPDTSLWTLIIL 186 Query: 187 TVWQFGSTMVIFLAGLKQIPNDLYEASSVDGANKITQFFRITLPMLSPILYFNLIMAVIG 246 VWQFG+ MVIFLAGL+QIP+++ EA+ VDGA + +F RITLP+LSPI++FNL++ +IG Sbjct: 187 RVWQFGAPMVIFLAGLRQIPSEVLEAAQVDGAGAVRRFLRITLPLLSPIVFFNLVLQIIG 246 Query: 247 AFQMFTSAFVIT--NGGPMNSTYVYAMYLYERAFSRYELGYASALAWIMLVAIIAATVLI 304 +FQ FT A++++ +GGP +ST Y +YLY+ AF + +GYA+A+AW++L+ I A T Sbjct: 247 SFQAFTPAYIVSGGSGGPADSTLFYTLYLYQEAFGNFRMGYAAAMAWVLLLVIAAFTAAN 306 Query: 305 SYTSKYWVFYETDTGGKK 322 S++WVFYE+DTGG + Sbjct: 307 FLMSRFWVFYESDTGGSR 324 >Q5WL27.1 Q5WL27_BACSK Lactose ABC transporter permease Length = 314 Score = 338 bits (868), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 163/313 (52%), Positives = 214/313 (68%) Query: 7 RPPAAIAQSAAKTKKSSYFRSRWNAPLAGYLFISPWLIGFLALTAYPLFLSLYYSFTDYT 66 +P I +K+S W L G+ FISPWL+GFL P+ SLYYSFTD+ Sbjct: 2 KPSETIRAEYPPNRKTSAKWRVWKQYLVGFAFISPWLVGFLGFVIGPMIASLYYSFTDFD 61 Query: 67 LLQPMKWIGTRNYERIFTADPKFIQSAKVTFMYVLASVPLKLIAALFVAIILSRAVKGIS 126 +L W+G NY +FT DP+F S VT ++V S P+KL AL +A++ + KG Sbjct: 62 MLTSPNWVGLDNYITMFTNDPRFRTSITVTLVFVFVSTPIKLAFALLIAMLFNNKRKGSG 121 Query: 127 FYRTAIYFPSLIGGSIGVSLLWRNIFGVDGIFNKLIGVFGFEGKGWITSPDTALSTLILL 186 FY T Y PS+IGGS+ V+++WR +FG G N ++ FG EGK WI SPD AL LILL Sbjct: 122 FYTTLFYIPSIIGGSVAVAVMWRQLFGGQGAINDILLFFGVEGKSWIASPDHALMVLILL 181 Query: 187 TVWQFGSTMVIFLAGLKQIPNDLYEASSVDGANKITQFFRITLPMLSPILYFNLIMAVIG 246 +WQFGS M+IFLAGL+QIP DLYEA+SVDGA K +F IT+P+L+P+++FNLIM +IG Sbjct: 182 VIWQFGSPMIIFLAGLRQIPEDLYEAASVDGAGKFRKFLSITIPLLTPVIFFNLIMQMIG 241 Query: 247 AFQMFTSAFVITNGGPMNSTYVYAMYLYERAFSRYELGYASALAWIMLVAIIAATVLISY 306 AF +FT AFV T GGP++ T YA+YLYERAF+ YE+GYASA+AW +L+ I T L+ Y Sbjct: 242 AFLVFTQAFVTTQGGPLDRTLFYALYLYERAFTNYEMGYASAMAWTLLIVIAIVTGLLFY 301 Query: 307 TSKYWVFYETDTG 319 +SK WVFYE++ G Sbjct: 302 SSKKWVFYESEKG 314 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.140 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,880,312,625 Number of extensions: 74751132 Number of successful extensions: 288327 Number of sequences better than 1.0e-05: 5995 Number of HSP's gapped: 279103 Number of HSP's successfully gapped: 6008 Length of query: 324 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 188 Effective length of database: 1,779,438,163 Effective search space: 334534374644 Effective search space used: 334534374644 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)