BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0284.0004.p (281 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAH4.1 B3KAH4_9BACI Binding-protein-dependent transport system... 480 e-134 B1D597.1 B1D597_9BACL Binding-protein-dependent transport system... 362 2e-98 B1DB01.1 B1DB01_9BACL Binding-protein-dependent transport system... 309 1e-82 Q9KDU2.1 Q9KDU2_BACHD Lactose transport system (Permease) 287 8e-76 Q5WL26.1 Q5WL26_BACSK Lactose ABC transporter permease 286 2e-75 >B3KAH4.1 B3KAH4_9BACI Binding-protein-dependent transport systems inner membrane component Length = 281 Score = 480 bits (1236), Expect = e-134, Method: Compositional matrix adjust. Identities = 228/281 (81%), Positives = 253/281 (90%) Query: 1 MTTLKWKPAFRHLFMILFSLIMVYPVLWWVGASLKHSTELGSPSIFPTAPQWSNFTKGWT 60 MT KW P RH +I+FSLIMVYPV+WW+GASLK + EL SPSIFP+ PQWSN+T GW Sbjct: 1 MTRRKWNPVIRHSVLIMFSLIMVYPVIWWIGASLKTNQELSSPSIFPSVPQWSNYTNGWN 60 Query: 61 SVPGHTFTDFYLNTFGLEFAVIVATLLSCTLVAFGFARLDFPLKNFWFSILMLTLMMPGQ 120 SVPG TFTDFYLNTF L AVI+ T++SCTLVAFGFARLDFPL+ FWFS+LMLTLM+PGQ Sbjct: 61 SVPGFTFTDFYLNTFYLITAVIIVTVISCTLVAFGFARLDFPLRGFWFSLLMLTLMLPGQ 120 Query: 121 VLIIPQYALFHQLGWVNTYLPFIVPHLLATGAGGSFFVFLLIQFIRGVPRELDESAKIDG 180 VLIIPQYA+FHQ GWVNTYLPFIVPH LA GAGG+FFVFLL+QFIRG+PRELDESAKIDG Sbjct: 121 VLIIPQYAMFHQFGWVNTYLPFIVPHALAGGAGGTFFVFLLVQFIRGIPRELDESAKIDG 180 Query: 181 CSWFGIFWRVVMPLAFPAIVTVTIFCFLWNWDDFLGHLLYINSVDKYTVGLALRMINDSQ 240 CSWFGI+WR+VMPL P+IVTV IFCFLWNWDDFLGHLLYINSVDKYTVGLALRMINDSQ Sbjct: 181 CSWFGIYWRIVMPLMKPSIVTVIIFCFLWNWDDFLGHLLYINSVDKYTVGLALRMINDSQ 240 Query: 241 SAAEWGQLLAMSLVSILPATIVFMFLQKYFVEGIATTGIKG 281 AEWGQLLAMSLVSILPAT++F+F QKYFVEG+ATTG+KG Sbjct: 241 GGAEWGQLLAMSLVSILPATLLFIFAQKYFVEGVATTGLKG 281 >B1D597.1 B1D597_9BACL Binding-protein-dependent transport systems inner membrane component Length = 282 Score = 362 bits (929), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 156/272 (57%), Positives = 215/272 (79%) Query: 10 FRHLFMILFSLIMVYPVLWWVGASLKHSTELGSPSIFPTAPQWSNFTKGWTSVPGHTFTD 69 F H +++ SLIM+YP+ WWVGASLK + E+ S ++FP P W+NF+ GW S P TFT Sbjct: 11 FNHASLLIISLIMMYPIFWWVGASLKTNEEMRSANLFPKVPHWANFSDGWVSTPNFTFTH 70 Query: 70 FYLNTFGLEFAVIVATLLSCTLVAFGFARLDFPLKNFWFSILMLTLMMPGQVLIIPQYAL 129 FY+NTF L ++ ++++C+LVAF FARLDFPL+ WF+I+++TLM+P QV ++PQY + Sbjct: 71 FYVNTFELIIGILFTSVVACSLVAFAFARLDFPLRKMWFAIMLVTLMLPTQVTVVPQYVM 130 Query: 130 FHQLGWVNTYLPFIVPHLLATGAGGSFFVFLLIQFIRGVPRELDESAKIDGCSWFGIFWR 189 F + GW+NTYLPF +PHLLA G GG FF++LL+QF+R VP+ELDE+AKIDGCSWFGI+WR Sbjct: 131 FSKWGWINTYLPFYIPHLLAGGIGGPFFIYLLVQFMRSVPKELDEAAKIDGCSWFGIYWR 190 Query: 190 VVMPLAFPAIVTVTIFCFLWNWDDFLGHLLYINSVDKYTVGLALRMINDSQSAAEWGQLL 249 +++PL PA+VTV I+CFLWNWDDF G +LYINS++ YT+ LAL++ DSQ + WGQLL Sbjct: 191 IMIPLVKPALVTVMIYCFLWNWDDFFGQMLYINSINHYTINLALKLFIDSQGSVPWGQLL 250 Query: 250 AMSLVSILPATIVFMFLQKYFVEGIATTGIKG 281 AMSL SI+P+ ++F Q++FVEG++ +KG Sbjct: 251 AMSLCSIVPSVVIFFLAQRHFVEGMSAGALKG 282 >B1DB01.1 B1DB01_9BACL Binding-protein-dependent transport systems inner membrane component Length = 278 Score = 309 bits (792), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 155/279 (55%), Positives = 212/279 (75%), Gaps = 4/279 (1%) Query: 3 TLKWKPAFRHLFMILFSLIMVYPVLWWVGASLKHSTELGSPSIFPTAPQWSNFTKGWTSV 62 TL+ HL +I FSL+M+YPV+WWVGASLK + E+ SPSI+P+ P W N+TKGW Sbjct: 4 TLRNYKVTSHLLLIAFSLLMLYPVIWWVGASLKTTAEMSSPSIWPSTPLWENYTKGWQFS 63 Query: 63 PGHTFTDFYLNTFGLEFAVIVATLLSCTLVAFGFARLDFPLKNFWFSILMLTLMMPGQVL 122 P TF F+ NT +EF ++ +L+ +VA+GFARL+F + FSIL+LT+M+PGQV Sbjct: 64 PEFTFGRFFGNTLLMEFWNVLGGVLTSAIVAYGFARLNFKFRGALFSILLLTMMLPGQVT 123 Query: 123 IIPQYALFHQLGWVNTYLPFIVPHLLATGAGGSFFVFLLIQFIRGVPRELDESAKIDGCS 182 I+PQY L++++ +++Y+P ++PH GA FF+FLL+QFIRG+PRELDE+AKIDG S Sbjct: 124 IVPQYILYNKMQLMDSYIPLVLPHFFGGGA---FFIFLLVQFIRGLPRELDEAAKIDGAS 180 Query: 183 WFGIFWRVVMPLAFPAIVTVTIFCFLWNWDDFLGHLLYINSVDKYTVGLALRMINDSQSA 242 +GIF+R+V+PL PA+VTV IF F+W+WDDF +LY+NSV K+TVGLALRM D Q Sbjct: 181 VYGIFYRIVLPLIKPALVTVAIFTFIWSWDDFFAQMLYLNSVGKFTVGLALRMYVD-QFE 239 Query: 243 AEWGQLLAMSLVSILPATIVFMFLQKYFVEGIATTGIKG 281 +WG+LLAMSL+S++P+ ++F F QK FVEGIATTG+KG Sbjct: 240 IQWGRLLAMSLLSVMPSVVLFFFAQKQFVEGIATTGLKG 278 >Q9KDU2.1 Q9KDU2_BACHD Lactose transport system (Permease) Length = 281 Score = 287 bits (734), Expect = 8e-76, Method: Compositional matrix adjust. Identities = 142/269 (52%), Positives = 188/269 (69%), Gaps = 5/269 (1%) Query: 14 FMILFSLIMVYPVLWWVGASLKHSTELGS--PSIFPTAPQWSNFTKGWTSVPGHTFTDFY 71 F+I F IMVYP+LW V +SLK ++E+ S S+ P+ WSN+ GW F ++ Sbjct: 16 FVIAFGFIMVYPILWMVASSLKPASEIFSQASSLIPSEFVWSNYPDGWAGFGRTGFDIYF 75 Query: 72 LNTFGLEFAVIVATLLSCTLVAFGFARLDFPLKNFWFSILMLTLMMPGQVLIIPQYALFH 131 N+F + +V++ +LS ++VA+GFARL F K F+ L+ T+M+P Q+ +IPQY LFH Sbjct: 76 KNSFIITTSVVIGAILSSSIVAYGFARLQFKFKKVLFACLIATVMLPVQITLIPQYILFH 135 Query: 132 QLGWVNTYLPFIVPHLLATGAGGSFFVFLLIQFIRGVPRELDESAKIDGCSWFGIFWRVV 191 LGWVNT+ P IVP L G FF+FLLIQFIRG+PRELDE+A IDGCS FG+FWRV+ Sbjct: 136 NLGWVNTFYPLIVPAFLG---GTPFFIFLLIQFIRGIPRELDEAAIIDGCSTFGVFWRVI 192 Query: 192 MPLAFPAIVTVTIFCFLWNWDDFLGHLLYINSVDKYTVGLALRMINDSQSAAEWGQLLAM 251 +PL PA+VTV IF F+W WDDFL L+Y+N D TV L LR D++S WG LLAM Sbjct: 193 LPLLKPALVTVAIFAFMWTWDDFLAPLIYLNKTDIQTVALGLRNFMDAESGTSWGALLAM 252 Query: 252 SLVSILPATIVFMFLQKYFVEGIATTGIK 280 S +S+LP I+F+F Q+Y VEGI+TTG+K Sbjct: 253 STLSLLPQFIIFLFFQRYLVEGISTTGLK 281 >Q5WL26.1 Q5WL26_BACSK Lactose ABC transporter permease Length = 280 Score = 286 bits (731), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 144/281 (51%), Positives = 190/281 (67%), Gaps = 5/281 (1%) Query: 2 TTLKWKPAFRHLFMILFSLIMVYPVLWWVGASLKHSTEL--GSPSIFPTAPQWSNFTKGW 59 T ++K H ++LF M+YPVLW + +S+K E+ + S+ P+A W N+ +GW Sbjct: 3 TKRRFKTFLYHFTVLLFGFFMLYPVLWMISSSVKPPAEIFQQAASLLPSAFHWDNYVEGW 62 Query: 60 TSVPGHTFTDFYLNTFGLEFAVIVATLLSCTLVAFGFARLDFPLKNFWFSILMLTLMMPG 119 F + N+ + I+ L+S + VAFGFARL FPLKN F+ L+ TLM+P Sbjct: 63 KGFGSVGFDQIFRNSLFVSSMNIIGALISSSFVAFGFARLKFPLKNILFACLIGTLMLPM 122 Query: 120 QVLIIPQYALFHQLGWVNTYLPFIVPHLLATGAGGSFFVFLLIQFIRGVPRELDESAKID 179 Q+ +IPQY LF+ GWVNT+LP IVP + G FF+FL++QFIRG+PRELDE+A ID Sbjct: 123 QITLIPQYILFNYFGWVNTFLPLIVPAFVG---GTPFFIFLMVQFIRGIPRELDEAAVID 179 Query: 180 GCSWFGIFWRVVMPLAFPAIVTVTIFCFLWNWDDFLGHLLYINSVDKYTVGLALRMINDS 239 GCS GIFWR++MPL P +VTVTIF FLW+WDDF G L+Y+N YTVGL L DS Sbjct: 180 GCSTLGIFWRIIMPLCKPVLVTVTIFAFLWSWDDFFGGLIYLNDPSLYTVGLGLASFLDS 239 Query: 240 QSAAEWGQLLAMSLVSILPATIVFMFLQKYFVEGIATTGIK 280 SA+ WG LLAM+ +S++P IVF+F QKY V+GIATTGIK Sbjct: 240 TSASSWGPLLAMATLSLVPQFIVFLFFQKYLVQGIATTGIK 280 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.144 0.482 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,716,069,394 Number of extensions: 70896329 Number of successful extensions: 254283 Number of sequences better than 1.0e-05: 6052 Number of HSP's gapped: 243590 Number of HSP's successfully gapped: 6052 Length of query: 281 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 147 Effective length of database: 1,798,280,193 Effective search space: 264347188371 Effective search space used: 264347188371 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)