BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0284.0003.p (578 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAH5.1 B3KAH5_9BACI Histidine kinase internal region 565 e-159 B1D596.1 B1D596_9BACL Histidine kinase internal region 380 e-103 Q9KDT9.1 Q9KDT9_BACHD BH1122 protein 327 3e-87 Q5WL30.1 Q5WL30_BACSK Two-component sensor histidine kinase 301 2e-79 B1DHC4.1 B1DHC4_9BACL Integral membrane sensor signal transducti... 197 3e-48 >B3KAH5.1 B3KAH5_9BACI Histidine kinase internal region Length = 574 Score = 565 bits (1457), Expect = e-159, Method: Compositional matrix adjust. Identities = 287/573 (50%), Positives = 387/573 (67%), Gaps = 3/573 (0%) Query: 1 LINPFKKYRIDRLFFHSFAIVLIALIVVTAWTSYSNSSKALVQTTSHYQQQLLDELNNEI 60 +INPFKKYRID LF FA ++ ++ +T WTSY +S+ L + TS+YQ++LL+ELNNEI Sbjct: 1 MINPFKKYRIDSLFIICFAGLITIVLAITVWTSYVMTSRELAENTSYYQKRLLEELNNEI 60 Query: 61 TTKLVMIEQISLSSSRDSELTNFLLNRQDEFERYRRRAGVESALANLTYAIPLIQGIDLY 120 T +L IEQISL++SRD++L FL ++DEF YRR G++ ALA+LTY+IP IQ IDLY Sbjct: 61 TGRLTSIEQISLTTSRDNDLLTFLSGKEDEFNHYRRSKGIQEALAHLTYSIPQIQAIDLY 120 Query: 121 MDNPIPSERQSYIQFRNLADLNKQVGAGCLEKSDFCWSREYNFPSAQGEVPVLSFSRKII 180 M+ P +RQSYIQF L + + L+ +DF WS E++ + G VPVLSFSRK+ Sbjct: 121 MEQPEWGDRQSYIQFHGLTEAETKPWYPLLQNNDFSWSEEHDLTTYNGNVPVLSFSRKVY 180 Query: 181 SENDYLGVLVVHIKANEIRKLLTGNSDGSNRIMADSEGQQIMKIGDAMDQSDWSRWIDLK 240 + + YLG+LVVH+KA+ IR +L G S SNR++ D G++++ +G +M + DL+ Sbjct: 181 NNSRYLGILVVHVKADWIRGILEGYSKDSNRMLLDLNGRELLSVGKSMRDAGLQEQ-DLR 239 Query: 241 -SNKSGYVHIPATDGSRDTLLVYSRMDNSIWTLIEFTSWKQITASSLKLAEWIXXXXXXX 299 +++SG + ++L+VYS++ +S W L+E T W++IT+ S+KLA I Sbjct: 240 FADQSGVSRLHTQPHGVESLIVYSKVADSNWMLVEITPWERITSGSVKLAGAIITIGIGA 299 Query: 300 XXXXXXXTHFLSKQFTKPIKNLVTAMRMYSVGGDNVELPVDYENEFGYLFSGYRKQNXXX 359 T +LS+Q KPI LV AM+ Y VGG EL DY NEFG LF+G+R+ N Sbjct: 300 LLLAFLLTLWLSRQLLKPIAQLVRAMKNYDVGGKQAELAGDYTNEFGILFAGFRRLNERI 359 Query: 360 XXXXXXXXXXXXQQRKAEIEALQANINPHFLYNMLDQLNWMAIEAGQDELSRILELMGRM 419 QQRKAEIEALQANINPHFLYN LDQLNWMAI GQ+++SRILELMGRM Sbjct: 360 QALYASLAQRYEQQRKAEIEALQANINPHFLYNTLDQLNWMAIAEGQEKMSRILELMGRM 419 Query: 420 FRIGLSNGNSFITIADELLHIECYLEIQQLRWGEGLEYRIEAAPELQELYIPKLTLQPFV 479 FRIGLS+G SFITI +EL H+ CYLEIQQ+RW +GLEY + A+ E++ L+IPK+TLQPFV Sbjct: 420 FRIGLSHGESFITIEEELTHVACYLEIQQIRWEDGLEYEVHASQEIRNLFIPKMTLQPFV 479 Query: 480 ENSIVHGFNKQRSGYVSIVMERLDDALHIVIDDNGAGLKQPGLNSQQRHTGGYGIRNVRE 539 ENSI+HGFN +++G + I E + + I D+G+GL P R GGYGIRNVRE Sbjct: 480 ENSIIHGFNSRQNGRIHIKAEEEGGRIRLTIIDDGSGLA-PLSKGNHRRKGGYGIRNVRE 538 Query: 540 RVAGYFGESYGITLKEREEGGTRVEIMLPLLTE 572 R + +F + Y I +K+ E+GG V I P L E Sbjct: 539 RFSAFFNDDYEIIVKDGEKGGVVVSISFPRLEE 571 >B1D596.1 B1D596_9BACL Histidine kinase internal region Length = 584 Score = 380 bits (975), Expect = e-103, Method: Compositional matrix adjust. Identities = 218/576 (37%), Positives = 323/576 (56%), Gaps = 19/576 (3%) Query: 2 INPFKKYRIDRLFFHSFAIVLIALIVVTAWTSYSNSSKALVQTTSHYQQQLLDELNNEIT 61 +NPFK RI+ +FF FA+++ L+ + SY N SK + TS+YQQ++L E++ ++T Sbjct: 3 LNPFKTMRIEYVFFFGFALIVTILVAIVVSFSYYNGSKEIADNTSYYQQKVLVEIHKKLT 62 Query: 62 TKLVMIEQISLSSSRDSELTNFL--LNRQDEFERYRRRAGVESALANLTYAIPLIQGIDL 119 T L IEQ S ++S + TN ++ +++++ R +AG+ + L N + +P++ I + Sbjct: 63 TNLKDIEQSSNTASLN--FTNMYEEMSGGNKYDQVRGQAGIRAQLNNFVFGMPIVHSIHV 120 Query: 120 YMDNPIPSERQSYIQFRNLADLNKQVGAGCLEKSDFCWSREYNFPSAQGEVPVLSFSRKI 179 Y D Q ++QF L + K+ LE SD W E + G+ PV+S++RK+ Sbjct: 121 YSDLTPSYNVQEFVQFLPLDGIEKEAWYKDLEHSDEAWVSERIIKTNNGDTPVISYARKV 180 Query: 180 ISE-NDYLGVLVVHIKANEIRKLLTGNSDGSNRIMADSEGQQIMKIGDA----MDQSDWS 234 + N V+V ++K + + L+T SN + D+ G+ I IG A Q++ Sbjct: 181 YNRFNRAFAVMVFNVKVPDFQSLITTEDSRSNLALLDATGKLITYIGKADFFTSHQAEIK 240 Query: 235 RWIDLKSNKSGYVHIPATDGSRDTLLVYSRMDNSIWTLIEFTSWKQITASSLKLAEWIXX 294 ++ K++ + D LV++ +S W+L+E TSW+QITA SLK I Sbjct: 241 EQLEQKNS--------GAKRTGDEFLVWTSSLDSKWSLLEVTSWRQITAGSLKQTRTILA 292 Query: 295 XXXXXXXXXXXXTHFLSKQFTKPIKNLVTAMRMYSVGGDNVELPVDYENEFGYLFSGYRK 354 FLS+QF KPI L+ AM +S+ V LP DY+NEFG LF GY K Sbjct: 293 FGIVTIILILLLAVFLSRQFIKPIGLLLKAMSNFSLN-KRVPLPADYKNEFGTLFQGYDK 351 Query: 355 QNXXXXXXXXXXXXXXXQQRKAEIEALQANINPHFLYNMLDQLNWMAIEAGQDELSRILE 414 QQR AEI+ALQ INPHFLYN LDQ+NWMAIEA Q ++S +L Sbjct: 352 LLSRIEQLYGTLEEKHEQQRAAEIKALQMMINPHFLYNSLDQVNWMAIEADQPQISSMLS 411 Query: 415 LMGRMFRIGLSNGNSFITIADELLHIECYLEIQQLRWGEGLEYRIEAAPELQELYIPKLT 474 +G+ FR+ L+N NS + + +EL HIE YL+ Q++RW + L Y + E +LY+PK+ Sbjct: 412 HLGQFFRLSLTNTNSLVPLTEELTHIESYLQFQKIRWEDRLTYTLNVPEEAGKLYVPKVI 471 Query: 475 LQPFVENSIVHGFNKQRSGYVSIVMERLDDALHIVIDDNGAGLKQPGLNSQQRHTGGYGI 534 LQPFVEN+ VHGF+ ++ + IV+ D L IVI D+G L++ ++ GGYG+ Sbjct: 472 LQPFVENAFVHGFHGKQQASLDIVVSVGFDQLLIVIRDDGRKLRE-DWQEKRSAKGGYGL 530 Query: 535 RNVRERVAGYFGESYGITLKEREEGGTRVEIMLPLL 570 RNVRER+ FG YG TL EGGT I LP L Sbjct: 531 RNVRERIEALFGNKYGFTLSNSPEGGTEASIRLPRL 566 >Q9KDT9.1 Q9KDT9_BACHD BH1122 protein Length = 586 Score = 327 bits (837), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 203/568 (35%), Positives = 301/568 (52%), Gaps = 7/568 (1%) Query: 5 FKKYRIDRLFFHSF-AIVLIALIVVTAWTSYSNSSKALVQTTSHYQQQLLDELNNEITTK 63 FK+Y+I +FF SF A +L+A IV+ + SY S +VQ T+ YQ+QLL L+N+I+ Sbjct: 4 FKRYQIRHIFFTSFFAFMLLAFIVIIS-VSYRYSVNEMVQATTEYQEQLLTLLSNDISNY 62 Query: 64 LVMIEQISLSSSRDSELTNFLLNRQDEFERYRRRAGVESALANLTYAIPLIQGIDLYMDN 123 L + IS+ SR S L +D + + + L+N+ Y++P + I++Y+ + Sbjct: 63 LQSYDDISVGLSRQSNFRQALKGSEDPYTYNQLFQSLSRDLSNIVYSVPGLHSIEVYLHS 122 Query: 124 PIPSERQSYIQFRNLADLNKQVGAGCLEKSDFCWSREYNFPSAQGEVPVLSFSRKII-SE 182 P SE Q I + L + + L+ + W E + GE VLS +R + + Sbjct: 123 PPHSEIQQPINYLPLEAVEEMGWTKRLDGKNSVWIGERVSYTMAGEERVLSLARNVFQAR 182 Query: 183 NDYLGVLVVHIKANEIRKLLTGNSDGSNRIMADSEGQQIMKIGDAMDQSDWSRWIDLKSN 242 + GVLV+++ A+ I L S SN ++ D +G+ + + +S + D Sbjct: 183 GEIEGVLVLNVHASTIETWLKQKSQESNLMIIDKQGRFVATSDPQHESVLYSPYFDQYLP 242 Query: 243 KSGYVHIPATDGSRDTLLVYSRMDNSIWTLIEFTSWKQITASSLKLAEWIXXXXXXXXXX 302 + D D L V + N+ WTL + T + ++T S ++A + Sbjct: 243 YQPTMQTGENDKDEDALEVSMPIPNTEWTLKQVTPYSELTEGSRRMAAILVIIGAIMIVV 302 Query: 303 XXXXTHFLSKQFTKPIKNLVTAMRMYSVGGDNVELPVDYENEFGYLFSGYRKQNXXXXXX 362 T+ L+K+FT PI L + Y + ELP DY NEFG LF GY+ Sbjct: 303 ALLVTYSLTKKFTTPIYELKNVLSKYPDQDLSGELPSDYRNEFGELFEGYKGLIRRNNEL 362 Query: 363 XXXXXXXXXQQRKAEIEALQANINPHFLYNMLDQLNWMAIEAGQDELSRILELMGRMFRI 422 +QR+AEI+ALQANINPHFLYN LDQLNWMAIE G +S +LE +G+M RI Sbjct: 363 YRSLHRQYKRQREAEIKALQANINPHFLYNTLDQLNWMAIEKGDRTMSHMLENLGQMLRI 422 Query: 423 GLSNGNSFITIADELLHIECYLEIQQLRWGEGLEYRIEAAPELQELYIPKLTLQPFVENS 482 GLSNG S + + E+ +++ YL+IQ+L+ GE L Y ++ L IPKLTLQPFVEN Sbjct: 423 GLSNGESILPLEKEVEYLKYYLKIQKLKMGERLTYTVDVPVMLYRYLIPKLTLQPFVENC 482 Query: 483 IVHGFNKQRSGYVSIVMERLDDALHIV--IDDNGAGLKQPGLNSQQRHTGGYGIRNVRER 540 +HGF R G +V+ ++D HIV I DNG G + + GGYGIRNV ER Sbjct: 483 FIHGFQDGRDG--EVVIRAMEDQNHIVMTIQDNGIGFQPVTTKRSKLDMGGYGIRNVMER 540 Query: 541 VAGYFGESYGITLKEREEGGTRVEIMLP 568 + YF + + E+GGT V+I++P Sbjct: 541 LDVYFDRYASVHVTGDEQGGTLVKIIIP 568 >Q5WL30.1 Q5WL30_BACSK Two-component sensor histidine kinase Length = 581 Score = 301 bits (770), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 185/569 (32%), Positives = 292/569 (51%), Gaps = 20/569 (3%) Query: 5 FKKYRIDRLFFHSFAIVLIALIVVTAWTSYSNSSKALVQTTSHYQQQLLDELNNEITTKL 64 FKK ++ + F S A+ +I L V SY + +TS +Q++LL NE+ ++ Sbjct: 2 FKKAKVKTILFLSIALFIIFLFFVVIGVSYRMVVSETIASTSAHQKELLHLYRNELDNQM 61 Query: 65 VMIEQISLSSSRDSELTNFLLNRQDEFERYRRRAGVESALANLTYAIPLIQGIDLYMDNP 124 ++E+ ++ SR+++L F+ NR + F R+R + L + + +I ID+YMDNP Sbjct: 62 KVLEETAVVISRNADLQAFINNRHEHFSYNRQRNNLHKYLHQVVLSSSMIDSIDIYMDNP 121 Query: 125 IPSERQSYIQFRNLADLNKQVGAGCLEKSDFCWSREYNFPSAQGEVPVLSFSRKIISEND 184 + Q I+F + D++ + +++ W E++ + E V+S+ K+ S Sbjct: 122 PTFDSQYPIRFLSKIDMHSHPKNKEVNEANEVWLGEHDKHLTEEE-KVVSYMCKLYSAKG 180 Query: 185 YL-GVLVVHIKANEIRKLLTGNSDGSN-RIMADSEGQQIMKIGDAMDQSDWSRWIDLKSN 242 L +LV++++ + L +N R++ G+ IM +R D + Sbjct: 181 TLKAMLVLNVRTRDFEALFVDRDHHANQRMLVGKNGEAIMA----------ARETDEEVG 230 Query: 243 KSGYVHIPATDGSRDT---LLVYSRMDNSIWTLIEFTSWKQITASSLKLAEWIXXXXXXX 299 + H G R+ L V+S ++ + W L+E T W ++T ++ + Sbjct: 231 RIVAEHSFNQGGKRELDQGLAVWSALERADWFLVEVTPWGELTKGGKRITAVLIAISLVA 290 Query: 300 XXXXXXXTHFLSKQFTKPIKNLVTAMRMYSVGGDNVELPVDYENEFGYLFSGYRKQNXXX 359 LS F+KPI L + V +P+ Y+NEFG LF G+ + Sbjct: 291 IAGALLFALLLSTAFSKPILQLRQGFVQFG-KSQKVNVPIGYQNEFGDLFEGFTEMTKEI 349 Query: 360 XXXXXXXXXXXXQQRKAEIEALQANINPHFLYNMLDQLNWMAIEAGQDELSRILELMGRM 419 ++ +A+I ALQANINPHFLYN LDQLNWMAI GQDE+S ++ELMG+M Sbjct: 350 HALHQSLEEEHRRKNEADITALQANINPHFLYNTLDQLNWMAIAKGQDEISEVIELMGKM 409 Query: 420 FRIGLSNGNSFITIADELLHIECYLEIQQLRWGEGLEYRIEAAPELQELYIPKLTLQPFV 479 RIGLS G SFI IADEL HI Y++IQQ + + I+ EL Y+PK TLQP V Sbjct: 410 LRIGLSKGKSFIPIADELDHIRYYMQIQQCTKSVDISFEIDVPSELHHYYVPKFTLQPIV 469 Query: 480 ENSIVHGFNKQRSGYVSIVMERLDDALHIVIDDNGAGLKQPGLNSQQRHTGGYGIRNVRE 539 EN+IVHGF++++ G + I + AL + + D+G G Q ++ GYG++NV E Sbjct: 470 ENAIVHGFHRRQRGMIDISIRPYKQALQLQVKDDGVGFDQKEPIPRK---NGYGLQNVAE 526 Query: 540 RVAGYFGESYGITLKEREEGGTRVEIMLP 568 R+ +FGE IT+ GT V I +P Sbjct: 527 RLRAFFGEEGTITVYSAIGAGTTVLIEIP 555 >B1DHC4.1 B1DHC4_9BACL Integral membrane sensor signal transduction histidine kinase Length = 611 Score = 197 bits (500), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 122/375 (32%), Positives = 196/375 (52%), Gaps = 25/375 (6%) Query: 212 IMADSEGQQIMKIG-----DAMDQSDWSRWIDLKSNKSGYVHIPATDGSRDTLLVYSRMD 266 ++ DS G+ +++ G D + D R K+ + DG LL YS+ + Sbjct: 226 VILDSTGEPLLEAGKPFFKDGQSELDAIR----KAIPESGSKVVKLDG-ESLLLTYSKGE 280 Query: 267 NSIWTLIEFTSWKQITASSLKLAEWIXXXXXXXXXXXXXXTHFLSKQFTKPIKNLVTAMR 326 N W + +T + +++ L+ T PIKNL+ +MR Sbjct: 281 NG-WQYLYAVPLSTLTKELNSIKQFVMLLIAVWLVMSIPVMLLLTMFLTAPIKNLLLSMR 339 Query: 327 MYSVGGDNVELPVDYENEFGYLFSGYRKQNXXXXXXXXXXXXXXXQQRKAEIEALQANIN 386 + G + ++ V Y +E GYL GY ++++AE++ALQA IN Sbjct: 340 RFQNGQFDEKVDVKYGDEIGYLTQGYNSMVESIKALVDDAYVLRLREQEAELKALQAQIN 399 Query: 387 PHFLYNMLDQLNWMAIEAGQDELSRILELMGRMFRIGLSNGNSFITIADELLHIECYLEI 446 PHFLYNMLD + W A AGQ+++S ++ + R+FR+ L+ G SF +A E +E YL + Sbjct: 400 PHFLYNMLDTIFWEAESAGQEKISEMIINLSRLFRLSLNRGKSFTNVAKERELLELYLSL 459 Query: 447 QQLRWGEGLEYRIEAAPELQELYIPKLTLQPFVENSIVHGFNKQR-SGYVSIVMERLDDA 505 Q++R+ + LEY ++ +P+L E I KL LQPFVEN+IVHG ++R G+V + R D Sbjct: 460 QKMRFRDVLEYEVDISPDLGEYVILKLLLQPFVENAIVHGIERKRGGGFVRVTGTREGDR 519 Query: 506 LHIVIDDNGAGL------------KQPGLNSQQRHTGGYGIRNVRERVAGYFGESYGITL 553 L VI+DNG+G+ K+ +N Q T GY +RNV+ER+ Y+ + + +T Sbjct: 520 LKFVIEDNGSGMGEEELAKLTELPKESDINVGQ-DTSGYAVRNVQERLKHYYKDDFELTY 578 Query: 554 KEREEGGTRVEIMLP 568 + GTRV++++P Sbjct: 579 DSKPGQGTRVQLIIP 593 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,270,998,881 Number of extensions: 132381738 Number of successful extensions: 309000 Number of sequences better than 1.0e-05: 1967 Number of HSP's gapped: 305611 Number of HSP's successfully gapped: 1967 Length of query: 578 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 437 Effective length of database: 1,732,333,088 Effective search space: 757029559456 Effective search space used: 757029559456 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)