BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0282.0005.p (149 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB89.1 B3KB89_9BACI RNA methyltransferase, TrmH family, group 2 244 3e-63 B1DDH3.1 B1DDH3_9BACL RNA methyltransferase, TrmH family, group 2 241 1e-62 Q45091.1 Q45091_BACCI SerC phosphoserine aminotransferase and gl... 236 5e-61 Q3AD16.1 Q3AD16_CARHZ RNA methyltransferase, TrmH family, group 2 209 8e-53 A3DBN9.1 A3DBN9_CLOTH RNA methyltransferase, TrmH family, group 2 199 7e-50 >B3KB89.1 B3KB89_9BACI RNA methyltransferase, TrmH family, group 2 Length = 154 Score = 244 bits (622), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 115/148 (77%), Positives = 127/148 (85%) Query: 1 VLVEPEIPANTGNIARTCAATGTHLHLVRPLGFRTDDATLKRAGLDYWHAVHIEYHDSFG 60 VLVEPEIPANTGNIARTCAATGTHLHLVRPLGF TDD TLKRAGLDYW+AVHIEYHDSF Sbjct: 6 VLVEPEIPANTGNIARTCAATGTHLHLVRPLGFNTDDKTLKRAGLDYWYAVHIEYHDSFE 65 Query: 61 EVLEKYAEGRFFYATTKADKRYTDFTFRDGDFFVFGKETKGLSAEILEAGKETVMRMPMS 120 E+ Y EGR+FYATTKA KRY D ++DGDF VFGKETKGL E+L A E +RMPM+ Sbjct: 66 ELQALYPEGRYFYATTKATKRYMDIEYQDGDFLVFGKETKGLPRELLAANAEHCIRMPMT 125 Query: 121 EAVRSLNLSNSAAIIVYEALRQLNFPQL 148 + VRSLNLSNSAAI+VYEALRQL+FP + Sbjct: 126 DKVRSLNLSNSAAIMVYEALRQLDFPGM 153 >B1DDH3.1 B1DDH3_9BACL RNA methyltransferase, TrmH family, group 2 Length = 154 Score = 241 bits (616), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 114/148 (77%), Positives = 127/148 (85%) Query: 1 VLVEPEIPANTGNIARTCAATGTHLHLVRPLGFRTDDATLKRAGLDYWHAVHIEYHDSFG 60 VLVEPEIPANTGNIARTCAATG HLHLVRPLGF+TDD TLKRAGLDYWHAVH+EYHDSF Sbjct: 6 VLVEPEIPANTGNIARTCAATGAHLHLVRPLGFQTDDRTLKRAGLDYWHAVHVEYHDSFK 65 Query: 61 EVLEKYAEGRFFYATTKADKRYTDFTFRDGDFFVFGKETKGLSAEILEAGKETVMRMPMS 120 E+ + GRFFYA+T+A K YT+F FRDGDFFVFGKETKGL E+++A +T MRMPM+ Sbjct: 66 ELQDANPNGRFFYASTRATKVYTEFDFRDGDFFVFGKETKGLPQELIDANLDTCMRMPMT 125 Query: 121 EAVRSLNLSNSAAIIVYEALRQLNFPQL 148 VRSLNLSNSAAIIVYEALRQ FP + Sbjct: 126 GDVRSLNLSNSAAIIVYEALRQNKFPGM 153 >Q45091.1 Q45091_BACCI SerC phosphoserine aminotransferase and glnA glutamine synthetase, strain ATCC 21783 (Fragment) Length = 144 Score = 236 bits (602), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 110/139 (79%), Positives = 121/139 (87%) Query: 1 VLVEPEIPANTGNIARTCAATGTHLHLVRPLGFRTDDATLKRAGLDYWHAVHIEYHDSFG 60 VLVEPEIPANTGNIARTCAATGTHLHLVRPLGF TDD TLKRAGLDYW+AVHIEYHDSF Sbjct: 6 VLVEPEIPANTGNIARTCAATGTHLHLVRPLGFNTDDKTLKRAGLDYWYAVHIEYHDSFE 65 Query: 61 EVLEKYAEGRFFYATTKADKRYTDFTFRDGDFFVFGKETKGLSAEILEAGKETVMRMPMS 120 E+ +KY EGRFFY +TKA KRY D F+DGDF VFGKETKGL E+L A ++ +RMPM+ Sbjct: 66 ELQQKYPEGRFFYTSTKATKRYCDIAFQDGDFLVFGKETKGLPPELLAANPDSCIRMPMT 125 Query: 121 EAVRSLNLSNSAAIIVYEA 139 + VRSLNLSNSAAIIVYEA Sbjct: 126 DKVRSLNLSNSAAIIVYEA 144 >Q3AD16.1 Q3AD16_CARHZ RNA methyltransferase, TrmH family, group 2 Length = 153 Score = 209 bits (531), Expect = 8e-53, Method: Compositional matrix adjust. Identities = 96/146 (65%), Positives = 115/146 (78%) Query: 1 VLVEPEIPANTGNIARTCAATGTHLHLVRPLGFRTDDATLKRAGLDYWHAVHIEYHDSFG 60 VLVEPEIP NTGNIARTCAATG+ LHLV+PLGF T+D LKRAGLDYW V + YH++F Sbjct: 5 VLVEPEIPYNTGNIARTCAATGSTLHLVKPLGFSTEDKYLKRAGLDYWDKVRVIYHENFQ 64 Query: 61 EVLEKYAEGRFFYATTKADKRYTDFTFRDGDFFVFGKETKGLSAEILEAGKETVMRMPMS 120 E+ EKY EGRFFY +TK + Y + +++ GDF VFGKET+GL E+LEA KE R+PM Sbjct: 65 ELREKYPEGRFFYLSTKGKRYYHEVSYKPGDFLVFGKETQGLPQELLEANKEFTFRIPML 124 Query: 121 EAVRSLNLSNSAAIIVYEALRQLNFP 146 +RSLNLSN+ AI+VYEALRQL FP Sbjct: 125 PQIRSLNLSNTVAIVVYEALRQLQFP 150 >A3DBN9.1 A3DBN9_CLOTH RNA methyltransferase, TrmH family, group 2 Length = 164 Score = 199 bits (506), Expect = 7e-50, Method: Compositional matrix adjust. Identities = 96/148 (64%), Positives = 112/148 (75%) Query: 1 VLVEPEIPANTGNIARTCAATGTHLHLVRPLGFRTDDATLKRAGLDYWHAVHIEYHDSFG 60 VLVEPEIP NTGNIARTCAA G +LHLV+PLGF +D LKRAGLDYW V+I+Y+DSF Sbjct: 6 VLVEPEIPQNTGNIARTCAAVGANLHLVKPLGFSVEDRYLKRAGLDYWDKVNIKYYDSFS 65 Query: 61 EVLEKYAEGRFFYATTKADKRYTDFTFRDGDFFVFGKETKGLSAEILEAGKETVMRMPMS 120 E+ +KY E F+Y+TTKA K YTD + D F VFGKET GL E+L KE +R+PM Sbjct: 66 ELRDKYKEHIFYYSTTKAQKLYTDVKYPDDCFIVFGKETAGLPEELLVENKEWCIRIPMR 125 Query: 121 EAVRSLNLSNSAAIIVYEALRQLNFPQL 148 + +RSLNLSNS AIIVYEALRQ NF L Sbjct: 126 DDIRSLNLSNSVAIIVYEALRQNNFAGL 153 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.137 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 874,175,288 Number of extensions: 34678381 Number of successful extensions: 88592 Number of sequences better than 1.0e-05: 1355 Number of HSP's gapped: 87601 Number of HSP's successfully gapped: 1355 Length of query: 149 Length of database: 3,060,696,203 Length adjustment: 111 Effective length of query: 38 Effective length of database: 2,014,963,538 Effective search space: 76568614444 Effective search space used: 76568614444 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)