BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0281.0005.p (185 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q7NL16.1 Q7NL16_GLOVI Gll1310 protein 122 2e-26 Q01VS1.1 Q01VS1_SOLUE Alpha/beta hydrolase fold 119 9e-26 B8HT45.1 B8HT45_CYAP4 Alpha/beta hydrolase fold protein 118 2e-25 Q022C4.1 Q022C4_SOLUE Alpha/beta hydrolase fold 115 2e-24 Q8YVC0.1 Q8YVC0_ANASP All2058 protein 114 5e-24 >Q7NL16.1 Q7NL16_GLOVI Gll1310 protein Length = 341 Score = 122 bits (306), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 68/162 (41%), Positives = 97/162 (59%), Gaps = 7/162 (4%) Query: 6 GEFTNIGSHKL--FRMIEGGDERYLPVVFEHGCGSSALLWSLVAPEIARITTTIVYDRAG 63 G ++G H+L + GG V+ E G G+ +L W V E+A+ T T+ YDRAG Sbjct: 44 GRLVDVGGHRLHLLEVGTGGPT----VILEAGGGAWSLEWCRVQAEVAKFTRTVSYDRAG 99 Query: 64 YGWSELGPFPRTNERCISELYELLQIEGIKGPYILVGHSYGGLNVRMFTHKHPELVAGVV 123 YGWS+ G T+ + EL+ LL+ I GPYILVG S GGL++RMF H +P+ VAGVV Sbjct: 100 YGWSDAGLKNPTSRQVSEELHALLEKAEIAGPYILVGRSLGGLHMRMFAHLYPQEVAGVV 159 Query: 124 LVDSMHEDEMTARFPEEHRKGQLFAVKFFWFLQVLSKIGILK 165 LVD+ HEDE P+ G+ + W L++L+ IG+++ Sbjct: 160 LVDASHEDENRRILPQLRAAGERNLNRLGW-LRLLAPIGLIR 200 >Q01VS1.1 Q01VS1_SOLUE Alpha/beta hydrolase fold Length = 350 Score = 119 bits (299), Expect = 9e-26, Method: Compositional matrix adjust. Identities = 60/128 (46%), Positives = 79/128 (61%), Gaps = 2/128 (1%) Query: 5 RGEFTNIGSHKLFRMIEGGDERYLPVVFEHGCGSSALLWSLVAPEIARITTTIVYDRAGY 64 +G IGS +L G + V+ + G G AL W LV PE+A+ T YDRAGY Sbjct: 51 QGRSIQIGSLRLNLDCSG--RGGITVILDSGMGGPALDWILVQPEVAKFTRVCSYDRAGY 108 Query: 65 GWSELGPFPRTNERCISELYELLQIEGIKGPYILVGHSYGGLNVRMFTHKHPELVAGVVL 124 GWS+ P PRT+ + EL LL G KGP+++VGHS+GG NVR+F ++P VAG+VL Sbjct: 109 GWSDASPEPRTSRQIAGELKSLLDAAGEKGPFVMVGHSFGGYNVRVFAGRYPRDVAGMVL 168 Query: 125 VDSMHEDE 132 VD+ H DE Sbjct: 169 VDASHPDE 176 >B8HT45.1 B8HT45_CYAP4 Alpha/beta hydrolase fold protein Length = 332 Score = 118 bits (296), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 65/175 (37%), Positives = 96/175 (54%), Gaps = 2/175 (1%) Query: 6 GEFTNIGSHKLFRMIEGGDERYLPVVFEHGCGSSALLWSLVAPEIARITTTIVYDRAGYG 65 G+ + + L ++ G E V+ E G ++ L W LV PE+A+ T + YDRAGYG Sbjct: 37 GKMIEVNGYGLHHLVMG--EGQPAVILESGQAATHLDWQLVQPEVAKFTQVVSYDRAGYG 94 Query: 66 WSELGPFPRTNERCISELYELLQIEGIKGPYILVGHSYGGLNVRMFTHKHPELVAGVVLV 125 WS + PRT ++ I +L +LLQ GI+ PY+LVG S G+ VR+F +++PE V+G+VLV Sbjct: 95 WSHISADPRTGQQVIEDLRQLLQSAGIQPPYVLVGMSLSGIFVRLFAYQYPEEVSGMVLV 154 Query: 126 DSMHEDEMTARFPEEHRKGQLFAVKFFWFLQVLSKIGILKLLAVFIRRPLKTYFI 180 HE P + Q F V ++IG+L+LL F + P I Sbjct: 155 SVSHERMDEEMPPAMVKLNQQFEWLTIHIFPVAARIGLLRLLVKFNQLPFLQTLI 209 >Q022C4.1 Q022C4_SOLUE Alpha/beta hydrolase fold Length = 334 Score = 115 bits (287), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 63/162 (38%), Positives = 91/162 (56%), Gaps = 3/162 (1%) Query: 6 GEFTNIGSHKLFRMIEGGDERYLPVVFEHGCGSSALLWSLVAPEIARITTTIVYDRAGYG 65 G ++G H+L+ G E VV + G + W+ + E++R T YDRAGYG Sbjct: 44 GRLVDVGGHRLYLSCTG--EGSPTVVLDSALGVVSYSWTPIQAEVSRFTRVCSYDRAGYG 101 Query: 66 WSELGPFPRTNERCISELYELLQIEGIKGPYILVGHSYGGLNVRMFTHKHPELVAGVVLV 125 S+ GP PRT+++ SEL+ LL GPY+LVGHS GGL +R+FT HP VAG+VLV Sbjct: 102 LSDPGPLPRTSKQLASELHSLLGTAEEAGPYVLVGHSLGGLTIRLFTQAHPAEVAGLVLV 161 Query: 126 DSMHEDEMTARFPEEHRKGQLFAVKFFWFLQVLSKIGILKLL 167 DS E+ A E R + + F VL++ G+ +++ Sbjct: 162 DSASEN-AEAHLTPELRPLMMPPLTQMKFAGVLAEFGMHRIV 202 >Q8YVC0.1 Q8YVC0_ANASP All2058 protein Length = 331 Score = 114 bits (284), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 68/168 (40%), Positives = 100/168 (59%), Gaps = 5/168 (2%) Query: 2 QIDRGEFTNIGSHKLFRMIEGGDERYLPVVFEHGCGSSALLWSLVAPEIARITTTIVYDR 61 Q G+ ++G ++L R+ G R VV EH G + L+ EIA++T +YDR Sbjct: 32 QSPPGKLIDVGGYRL-RLYTAGHSRP-TVVLEHSLG--GIEGYLLVEEIAKLTRVCIYDR 87 Query: 62 AGYGWSELGPFPRTNERCISELYELLQIEGIKGPYILVGHSYGGLNVRMFTHKHPELVAG 121 AGYGWS+ P PRT+ + ++EL LL I GI+ PYILVG+S+G NVR++ H P+ V G Sbjct: 88 AGYGWSDNSPHPRTSRQIVAELDFLLTIAGIEPPYILVGNSFGSYNVRLYAHYFPDKVLG 147 Query: 122 VVLVDSMHEDEMTARFPEEHRKGQLFAVKFFWFLQVLSKIGILKLLAV 169 +VL D +HE M + + +LF V F+ + S +GI++LL V Sbjct: 148 IVLTDGLHETGML-KMSIYLQALKLFFVSGFFMSILASILGIIRLLNV 194 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.144 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,127,896,347 Number of extensions: 45596743 Number of successful extensions: 145988 Number of sequences better than 1.0e-05: 444 Number of HSP's gapped: 146958 Number of HSP's successfully gapped: 444 Length of query: 185 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 58 Effective length of database: 1,864,227,298 Effective search space: 108125183284 Effective search space used: 108125183284 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)