BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0281.0004.p (334 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q03658.1 Q03658_9BACT Precursor polypeptide (AA -37 to 1647) 388 e-106 Q9KFR4.1 Q9KFR4_BACHD Alpha-amylase G-6 281 4e-74 A8QWV3.1 A8QWV3_BACHD Alkaline and thermostable amylase 281 4e-74 B5MEM6.1 B5MEM6_BACHD Alkaline, thermotolerant, and maltotetraos... 281 5e-74 Q5WHK9.1 Q5WHK9_BACSK Alpha-amylase EC=3.2.1.1 280 1e-73 >Q03658.1 Q03658_9BACT Precursor polypeptide (AA -37 to 1647) Length = 1684 Score = 388 bits (996), Expect = e-106, Method: Compositional matrix adjust. Identities = 176/237 (74%), Positives = 199/237 (83%) Query: 95 QQEASPFSWDNANVYFVMTDRFNNGNTSNDGAYGRPKTDAWGSNVGTFHGGDIKGLTQKL 154 Q E PFSWDNANVYFV+TDRF NGNTSND +YGRP+ DAWG+N+GTFHGGDIKGLT+KL Sbjct: 45 QLENKPFSWDNANVYFVLTDRFYNGNTSNDNSYGRPQIDAWGTNIGTFHGGDIKGLTKKL 104 Query: 155 EEGYFTDLGTNAIWITAPWEQMHGWVGGKDGDFAHYGYHGYYALDFTAMDKSMGTIAEMR 214 EEGYFTDLGTNAIWI+APWEQMHGWVGGKDGDFAHYGYHGYY LDFTAMD++MGTI EMR Sbjct: 105 EEGYFTDLGTNAIWISAPWEQMHGWVGGKDGDFAHYGYHGYYGLDFTAMDQNMGTIDEMR 164 Query: 215 EFVDLAHAKGIRVVLDVVMNHVAYPTLVDMAEYGYGDRGGLAGSWIPDKGQGQNWHTHNN 274 EFVDLAH+ GIRVVLD+VMNHV YPT+VDM E+G+GD GGL W P++ QGQNWHTHN+ Sbjct: 165 EFVDLAHSLGIRVVLDIVMNHVGYPTIVDMHEFGFGDTGGLPRDWTPNQAQGQNWHTHND 224 Query: 275 IMNTNNXXXXXXXXXXXXIRANNIAGYDQCGGSDQTLCVGFLPDIKTESTSGXXXSP 331 IMN +N IRA+ AGYD CGGS+QT+C+GFLPDIKTE T+G P Sbjct: 225 IMNKDNEAAWANWWGSDWIRADETAGYDNCGGSEQTMCIGFLPDIKTEVTTGVDLPP 281 >Q9KFR4.1 Q9KFR4_BACHD Alpha-amylase G-6 Length = 958 Score = 281 bits (720), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 143/243 (58%), Positives = 176/243 (72%), Gaps = 9/243 (3%) Query: 90 SSGGQQQEASPFSWDNANVYFVMTDRFNNGNTSNDGAYGRPKTDAWGSNVGTFHGGDIKG 149 S GG+QQ FSWDNA VYF +TDRF++GN SN+ +YGRP+ DAWG N+GTFHGGD++G Sbjct: 36 SQGGEQQS---FSWDNATVYFAITDRFHDGNPSNNQSYGRPQEDAWGQNIGTFHGGDLQG 92 Query: 150 LTQKLEEGYFTDLGTNAIWITAPWEQMHGWV-GGKDGDFAHYGYHGYYALDFTAMDKSMG 208 LT KL EGYFT+LG NAIWITAP+EQ+HGWV GG DGDFAHY YHGYYALD+T +D++MG Sbjct: 93 LTDKLNEGYFTELGINAIWITAPYEQVHGWVGGGSDGDFAHYAYHGYYALDYTMIDQNMG 152 Query: 209 TIAEMREFVDLAHAKGIRVVLDVVMNHVAYPTLVDMAEYGYGDRGGLAGSWIPDKGQGQN 268 T+ +MREFV AH +GIRVVLDVVMNH Y T+ DM EYG+G+ G++ W P GQGQN Sbjct: 153 TVEDMREFVHTAHEQGIRVVLDVVMNHPGYNTIKDMHEYGFGNP-GVSEHWTP--GQGQN 209 Query: 269 WHTHNNIMNTNNXXXXXXXXXXXXIRANNIAGYDQCGGSDQTLCVGFLPDIKTESTSGXX 328 WH + +N + IRA IAGY+ CG S+ T+C+ LPD +TE T Sbjct: 210 WHDVHQQINYED-AGAWRHWWGPWIRA-GIAGYESCGNSEITMCLAGLPDFRTELTHSVG 267 Query: 329 XSP 331 P Sbjct: 268 LPP 270 >A8QWV3.1 A8QWV3_BACHD Alkaline and thermostable amylase Length = 958 Score = 281 bits (720), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 143/243 (58%), Positives = 176/243 (72%), Gaps = 9/243 (3%) Query: 90 SSGGQQQEASPFSWDNANVYFVMTDRFNNGNTSNDGAYGRPKTDAWGSNVGTFHGGDIKG 149 S GG+QQ FSWDNA VYF +TDRF++GN SN+ +YGRP+ DAWG N+GTFHGGD++G Sbjct: 36 SQGGEQQS---FSWDNATVYFAITDRFHDGNPSNNQSYGRPQEDAWGQNIGTFHGGDLQG 92 Query: 150 LTQKLEEGYFTDLGTNAIWITAPWEQMHGWV-GGKDGDFAHYGYHGYYALDFTAMDKSMG 208 LT KL EGYFT+LG NAIWITAP+EQ+HGWV GG DGDFAHY YHGYYALD+T +D++MG Sbjct: 93 LTDKLNEGYFTELGINAIWITAPYEQVHGWVGGGSDGDFAHYAYHGYYALDYTMIDQNMG 152 Query: 209 TIAEMREFVDLAHAKGIRVVLDVVMNHVAYPTLVDMAEYGYGDRGGLAGSWIPDKGQGQN 268 T+ +MREFV AH +GIRVVLDVVMNH Y T+ DM EYG+G+ G++ W P GQGQN Sbjct: 153 TVEDMREFVHTAHEQGIRVVLDVVMNHPGYNTIKDMHEYGFGNP-GVSEHWTP--GQGQN 209 Query: 269 WHTHNNIMNTNNXXXXXXXXXXXXIRANNIAGYDQCGGSDQTLCVGFLPDIKTESTSGXX 328 WH + +N + IRA IAGY+ CG S+ T+C+ LPD +TE T Sbjct: 210 WHDVHQQINYED-AGAWRHWWGPWIRA-GIAGYESCGNSEITMCLAGLPDFRTELTHSVG 267 Query: 329 XSP 331 P Sbjct: 268 LPP 270 >B5MEM6.1 B5MEM6_BACHD Alkaline, thermotolerant, and maltotetraose-producing amylase Length = 958 Score = 281 bits (720), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 143/243 (58%), Positives = 176/243 (72%), Gaps = 9/243 (3%) Query: 90 SSGGQQQEASPFSWDNANVYFVMTDRFNNGNTSNDGAYGRPKTDAWGSNVGTFHGGDIKG 149 S GG+QQ FSWDNA VYF +TDRF++GN SN+ +YGRP+ DAWG N+GTFHGGD++G Sbjct: 36 SQGGEQQS---FSWDNATVYFAITDRFHDGNPSNNQSYGRPQEDAWGQNIGTFHGGDLQG 92 Query: 150 LTQKLEEGYFTDLGTNAIWITAPWEQMHGWV-GGKDGDFAHYGYHGYYALDFTAMDKSMG 208 LT KL EGYFT+LG NAIWITAP+EQ+HGWV GG DGDFAHY YHGYYALD+T +D++MG Sbjct: 93 LTDKLNEGYFTELGINAIWITAPYEQVHGWVGGGSDGDFAHYAYHGYYALDYTMIDQNMG 152 Query: 209 TIAEMREFVDLAHAKGIRVVLDVVMNHVAYPTLVDMAEYGYGDRGGLAGSWIPDKGQGQN 268 T+ +MREFV AH +GIRVVLDVVMNH Y T+ DM EYG+G+ G++ W P GQGQN Sbjct: 153 TVEDMREFVHTAHEQGIRVVLDVVMNHPGYNTIKDMHEYGFGNP-GVSEHWTP--GQGQN 209 Query: 269 WHTHNNIMNTNNXXXXXXXXXXXXIRANNIAGYDQCGGSDQTLCVGFLPDIKTESTSGXX 328 WH + +N + IRA IAGY+ CG S+ T+C+ LPD +TE T Sbjct: 210 WHDVHQQINYED-AGAWRHWWGPWIRA-GIAGYESCGNSEITMCLAGLPDFRTELTHSVG 267 Query: 329 XSP 331 P Sbjct: 268 LPP 270 >Q5WHK9.1 Q5WHK9_BACSK Alpha-amylase EC=3.2.1.1 Length = 955 Score = 280 bits (716), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 138/240 (57%), Positives = 171/240 (71%), Gaps = 8/240 (3%) Query: 94 QQQEASPFSWDNANVYFVMTDRFNNGNTSNDGAYGRPKTDAWGSNVGTFHGGDIKGLTQK 153 Q + PFSWDNA VYF +TDRFN+GN N+ +YGRP+ DAWG N+GTFHGGD++GLT K Sbjct: 35 QNSDQKPFSWDNATVYFAITDRFNDGNRGNNDSYGRPQKDAWGQNIGTFHGGDLQGLTDK 94 Query: 154 LEEGYFTDLGTNAIWITAPWEQMHGWV-GGKDGDFAHYGYHGYYALDFTAMDKSMGTIAE 212 L EGYFTDLG N IWI+AP+EQ+HGWV GG DGDFAHY YHGYYALD+T +D++MGT+ E Sbjct: 95 LNEGYFTDLGINTIWISAPYEQIHGWVGGGSDGDFAHYAYHGYYALDYTTVDQNMGTVEE 154 Query: 213 MREFVDLAHAKGIRVVLDVVMNHVAYPTLVDMAEYGYGDRGGLAGSWIPDKGQGQNWH-T 271 MREFV+ AH +GIRVVLDVVMNH Y T+ DM EYG+G+ G++ W P G+GQ WH Sbjct: 155 MREFVNTAHEQGIRVVLDVVMNHPGYNTIKDMNEYGFGNP-GVSEDWTP--GEGQTWHDV 211 Query: 272 HNNIMNTNNXXXXXXXXXXXXIRANNIAGYDQCGGSDQTLCVGFLPDIKTESTSGXXXSP 331 HN+I + +RA I GY+ CG S+ T+C+ LPD +TE T P Sbjct: 212 HNHIH--YDDASPWSTWWGPWVRA-GIGGYESCGNSETTMCLAGLPDFRTELTHSVGLPP 268 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,004,170,127 Number of extensions: 82039938 Number of successful extensions: 155081 Number of sequences better than 1.0e-05: 1417 Number of HSP's gapped: 154142 Number of HSP's successfully gapped: 1417 Length of query: 334 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 198 Effective length of database: 1,779,438,163 Effective search space: 352328756274 Effective search space used: 352328756274 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)