BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0029.p (411 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KII3.1 B3KII3_9BACI Aspartokinase EC=2.7.2.4 634 e-180 C0ZF36.1 C0ZF36_BREBN Aspartokinase 1 EC=2.7.2.4 464 e-129 C2N4U1.1 C2N4U1_BACCE Aspartokinase 1 454 e-126 C2XFF8.1 C2XFF8_BACCE Aspartokinase 1 453 e-125 C3EPB1.1 C3EPB1_BACTK Aspartokinase 1 453 e-125 >B3KII3.1 B3KII3_9BACI Aspartokinase EC=2.7.2.4 Length = 404 Score = 634 bits (1634), Expect = e-180, Method: Compositional matrix adjust. Identities = 308/406 (75%), Positives = 348/406 (85%), Gaps = 2/406 (0%) Query: 6 MGILVQKFGGTSLSTPAAREHVIRHVKRELASGFSLVVVVSAMGRKGEPYATDTLLDWAV 65 M ILVQKFGGTSLSTP AR HVI H++RELA+ + LVVVVSAMGR+GEPYATDTLLDW Sbjct: 1 MRILVQKFGGTSLSTPQARTHVIGHIQRELANHYQLVVVVSAMGRRGEPYATDTLLDWIG 60 Query: 66 QNGDALPSREKDLLMCCGEIISATTLCGLLEHEGVPATVLTGAQAGFLTDNNYGNARILD 125 NG+ALP RE+DLL+CCGEIISA+TLC LL+HEG+ ATVLTGAQAGFLTD+++GNARI D Sbjct: 61 HNGNALPDRERDLLLCCGEIISASTLCSLLQHEGITATVLTGAQAGFLTDDHFGNARIKD 120 Query: 126 VRTERILRELREHKVVIVTGFQGQTEAGDFTTLGRGGSDTSATALGAALRADMVDIYTDV 185 V+ ER+L+EL H+VVIVTGFQGQT++GDFTTLGRGGSDTSATALGAALRA+MVDIYTDV Sbjct: 121 VKPERVLKELESHQVVIVTGFQGQTDSGDFTTLGRGGSDTSATALGAALRAEMVDIYTDV 180 Query: 186 DGILTADPRIVEDAKQLSYVSYTEICNMAYQGAKVIHPRAVEIAMQAQIPVRVRSTFSEA 245 +GILTADPRIVEDAK LSYVSYTEICNMA+QGAKVIHPRAVEIAMQ+QIPVRVRSTFS+ Sbjct: 181 NGILTADPRIVEDAKPLSYVSYTEICNMAHQGAKVIHPRAVEIAMQSQIPVRVRSTFSDN 240 Query: 246 EGTLVTHPEGFSDVSHGVVDRFVTGIAYVSNVTQISVECPEGNGTGVQLQIFKSMADNGI 305 EGTLVT PEGF D+ G+VDRFVTG+AYVSN+TQISVEC G+QL++FKSMA++ I Sbjct: 241 EGTLVTQPEGFRDIQSGIVDRFVTGVAYVSNITQISVECDRTE--GLQLKVFKSMAEHSI 298 Query: 306 SVDFINVTPTEALYTVNDDKSEQAIAALQELGLRPSSLSGCAKXXXXXXXXXXXXXXMAR 365 SVDFINVTPT LYTV D SE+AI LQ L L+P SLSGCAK MA+ Sbjct: 299 SVDFINVTPTGVLYTVFDSDSEKAIEVLQSLDLKPKSLSGCAKVSVIGGGINGVPGIMAK 358 Query: 366 IVEALSSQNIQILQSADSNTTIWVLVKKEDMVQSLRALHAKFELHR 411 IVE+LS +I ILQSADSNTTIWVLVKKEDM Q+LRALH KFELHR Sbjct: 359 IVESLSEHDIPILQSADSNTTIWVLVKKEDMAQALRALHHKFELHR 404 >C0ZF36.1 C0ZF36_BREBN Aspartokinase 1 EC=2.7.2.4 Length = 408 Score = 464 bits (1193), Expect = e-129, Method: Compositional matrix adjust. Identities = 234/405 (57%), Positives = 296/405 (73%), Gaps = 1/405 (0%) Query: 6 MGILVQKFGGTSLSTPAAREHVIRHVKRELASGFSLVVVVSAMGRKGEPYATDTLLDWAV 65 M ILVQKFGG+SL+T R I H+++ + G++LVVVVSAMGRKG+PYATDTLL Sbjct: 1 MKILVQKFGGSSLTTEDCRMRAIYHMEKAIDEGYALVVVVSAMGRKGDPYATDTLLQLVR 60 Query: 66 QNGDALPSREKDLLMCCGEIISATTLCGLLEHEGVPATVLTGAQAGFLTDNNYGNARILD 125 NG+ LPSRE D+LM GEIISAT +C +L G+ AT+LTG QA +T +++ NA+I+ Sbjct: 61 ANGNQLPSREMDMLMHTGEIISATVMCSMLNARGIKATILTGGQANIVTSDDFTNAQIMS 120 Query: 126 VRTERILRELREHKVVIVTGFQGQTEAGDFTTLGRGGSDTSATALGAALRADMVDIYTDV 185 + RIL++L+E +VVIV GFQG+T + TTLGRGGSDT+ATALG AL+A+ VDI+TDV Sbjct: 121 IDPARILQDLQESQVVIVPGFQGRTAEWEITTLGRGGSDTTATALGVALKAETVDIFTDV 180 Query: 186 DGILTADPRIVEDAKQLSYVSYTEICNMAYQGAKVIHPRAVEIAMQAQIPVRVRSTFSEA 245 +GI+TADPRIVE+A++L V+YTEICNMA+ GAKVIHPRAVEIAM A +P+RVRSTFS+ Sbjct: 181 EGIMTADPRIVEEAQRLGMVTYTEICNMAHLGAKVIHPRAVEIAMHANVPIRVRSTFSDD 240 Query: 246 EGTLVTHPEGFSDVSHGVVDRFVTGIAYVSNVTQISVECPEGNGTGVQLQIFKSMADNGI 305 GTLVT + + V DR V GIA+V N+TQI V EG+ QLQ+FK+MA N I Sbjct: 241 PGTLVTTMLEIGKMGYTVNDRVVMGIAHVPNITQIKVANKEGS-YDTQLQVFKTMATNNI 299 Query: 306 SVDFINVTPTEALYTVNDDKSEQAIAALQELGLRPSSLSGCAKXXXXXXXXXXXXXXMAR 365 SVDFINV P YTV+D+ E+A L E+G P L CAK MAR Sbjct: 300 SVDFINVNPMGVAYTVHDEMGEKAARLLTEMGYEPQLLPHCAKVSVIGAGMTGVPGVMAR 359 Query: 366 IVEALSSQNIQILQSADSNTTIWVLVKKEDMVQSLRALHAKFELH 410 IVEAL+ ++IQILQSADS+TTIWVLV + DMV+++RALH +F LH Sbjct: 360 IVEALTQEDIQILQSADSHTTIWVLVHEMDMVKAVRALHQQFNLH 404 >C2N4U1.1 C2N4U1_BACCE Aspartokinase 1 Length = 413 Score = 454 bits (1168), Expect = e-126, Method: Compositional matrix adjust. Identities = 222/406 (54%), Positives = 292/406 (71%), Gaps = 2/406 (0%) Query: 6 MGILVQKFGGTSLSTPAAREHVIRHVKRELASGFSLVVVVSAMGRKGEPYATDTLLDWAV 65 M I+VQKFGGTS+ R+H + H+K+ LA+G+ +V VVSAMGRKGEPYATDTLL Sbjct: 4 MKIIVQKFGGTSVRDDNGRKHALHHIKKSLAAGYKVVTVVSAMGRKGEPYATDTLLSLVN 63 Query: 66 QNGDALPSREKDLLMCCGEIISATTLCGLLEHEGVPATVLTGAQAGFLTDNNYGNARILD 125 Q + RE+DLL+ CGE+ISA +L G+ A L GAQAGF+T++++ NA+I++ Sbjct: 64 QEESTISKREQDLLLSCGELISAIVFSNMLNENGIKAAALNGAQAGFVTNDDFTNAKIIE 123 Query: 126 VRTERILRELREHKVVIVTGFQGQTEAGDFTTLGRGGSDTSATALGAALRADMVDIYTDV 185 + +RI EL V++VTGFQGQT+ GD TTLGRGGSDTSA+ALG AL A+ +DI+TDV Sbjct: 124 MNCDRIHEELENVDVIVVTGFQGQTKKGDTTTLGRGGSDTSASALGVALHAEYIDIFTDV 183 Query: 186 DGILTADPRIVEDAKQLSYVSYTEICNMAYQGAKVIHPRAVEIAMQAQIPVRVRSTFSEA 245 +G++TADPRIV+DA+ L V+Y EICNMAYQGAKV+HPRAVEIAM A++P+RVRST+SE+ Sbjct: 184 EGVMTADPRIVKDARHLQTVTYNEICNMAYQGAKVVHPRAVEIAMHAKVPLRVRSTYSES 243 Query: 246 EGTLVTHPEGFSDVSHGVVDRFVTGIAYVSNVTQISVECPEGNGTGVQLQIFKSMADNGI 305 EGTL+ +G + V +R VTGIA+VSNVTQI V E +Q +FK MA+ GI Sbjct: 244 EGTLIAAYDGATK-GQDVEERPVTGIAHVSNVTQIKVLAKE-TAYDIQQHVFKEMANEGI 301 Query: 306 SVDFINVTPTEALYTVNDDKSEQAIAALQELGLRPSSLSGCAKXXXXXXXXXXXXXXMAR 365 SVD IN++PT YTVND+ S +A+ L++LG P CAK A+ Sbjct: 302 SVDLINISPTGVAYTVNDNVSARAVEVLKQLGYEPIVTEHCAKVSIVGAGMAGIPGVTAK 361 Query: 366 IVEALSSQNIQILQSADSNTTIWVLVKKEDMVQSLRALHAKFELHR 411 IV AL+ + IQILQSADS+TTIWVLVK+ D+V+++ ALH+ FEL + Sbjct: 362 IVTALAEKGIQILQSADSHTTIWVLVKETDLVEAVNALHSAFELSK 407 >C2XFF8.1 C2XFF8_BACCE Aspartokinase 1 Length = 413 Score = 453 bits (1166), Expect = e-125, Method: Compositional matrix adjust. Identities = 221/406 (54%), Positives = 293/406 (72%), Gaps = 2/406 (0%) Query: 6 MGILVQKFGGTSLSTPAAREHVIRHVKRELASGFSLVVVVSAMGRKGEPYATDTLLDWAV 65 M I+VQKFGGTS+ R+H + H+K+ LA+G+ +V VVSAMGRKGEPYATDTLL Sbjct: 4 MKIIVQKFGGTSVRDDNGRKHALHHIKKSLAAGYKVVTVVSAMGRKGEPYATDTLLSLVN 63 Query: 66 QNGDALPSREKDLLMCCGEIISATTLCGLLEHEGVPATVLTGAQAGFLTDNNYGNARILD 125 Q + RE+DLL+ CGE+ISA +L G+ A L GAQAGF+T++++ NA+I++ Sbjct: 64 QEESTISKREQDLLLSCGELISAIVFSNMLNENGIKAAALNGAQAGFVTNDDFTNAKIIE 123 Query: 126 VRTERILRELREHKVVIVTGFQGQTEAGDFTTLGRGGSDTSATALGAALRADMVDIYTDV 185 + +RI EL V++VTGFQGQT+ GD TTLGRGGSDTSA+ALG AL A+ +DI+TDV Sbjct: 124 MNCDRIHEELENVDVIVVTGFQGQTKKGDTTTLGRGGSDTSASALGVALHAEYIDIFTDV 183 Query: 186 DGILTADPRIVEDAKQLSYVSYTEICNMAYQGAKVIHPRAVEIAMQAQIPVRVRSTFSEA 245 +G++TADPRIV+DA+ L V+Y EICNMAYQGAKV+HPRAVEIAM A++P+RVRST+S++ Sbjct: 184 EGVMTADPRIVKDARHLQTVTYNEICNMAYQGAKVVHPRAVEIAMHAKVPLRVRSTYSDS 243 Query: 246 EGTLVTHPEGFSDVSHGVVDRFVTGIAYVSNVTQISVECPEGNGTGVQLQIFKSMADNGI 305 EGTL++ +G + V +R VTGIA+VSNVTQI V E +Q +FK MA+ GI Sbjct: 244 EGTLISAYDGATK-GQDVEERPVTGIAHVSNVTQIKVLAKE-TAYDIQQHVFKEMANEGI 301 Query: 306 SVDFINVTPTEALYTVNDDKSEQAIAALQELGLRPSSLSGCAKXXXXXXXXXXXXXXMAR 365 SVD IN++PT YTVND+ S +A+ L++LG P CAK A+ Sbjct: 302 SVDLINISPTGVAYTVNDNVSARAVEVLKQLGYEPIVTEHCAKVSIVGAGMAGYPGVTAK 361 Query: 366 IVEALSSQNIQILQSADSNTTIWVLVKKEDMVQSLRALHAKFELHR 411 IV AL+ + IQILQSADS+TTIWVLVK+ D+V+++ ALH+ FEL + Sbjct: 362 IVTALAEKGIQILQSADSHTTIWVLVKETDLVEAVNALHSAFELSK 407 >C3EPB1.1 C3EPB1_BACTK Aspartokinase 1 Length = 413 Score = 453 bits (1166), Expect = e-125, Method: Compositional matrix adjust. Identities = 222/406 (54%), Positives = 292/406 (71%), Gaps = 2/406 (0%) Query: 6 MGILVQKFGGTSLSTPAAREHVIRHVKRELASGFSLVVVVSAMGRKGEPYATDTLLDWAV 65 M I+VQKFGGTS+ R+H + H+K+ LA+G+ +V VVSAMGRKGEPYATDTLL Sbjct: 4 MKIIVQKFGGTSVRDDNGRKHALHHIKKSLAAGYKVVTVVSAMGRKGEPYATDTLLSLVN 63 Query: 66 QNGDALPSREKDLLMCCGEIISATTLCGLLEHEGVPATVLTGAQAGFLTDNNYGNARILD 125 Q + RE+DLL+ CGE+ISA +L G+ A L GAQAGF+T++++ NA+I++ Sbjct: 64 QEESTISKREQDLLLSCGELISAIVFSNMLNENGIKAAALNGAQAGFVTNDDFTNAKIIE 123 Query: 126 VRTERILRELREHKVVIVTGFQGQTEAGDFTTLGRGGSDTSATALGAALRADMVDIYTDV 185 + +RI EL V++VTGFQGQT+ GD TTLGRGGSDTSA+ALG AL A+ +DI+TDV Sbjct: 124 MNCDRIHEELENVDVIVVTGFQGQTKKGDTTTLGRGGSDTSASALGVALHAEYIDIFTDV 183 Query: 186 DGILTADPRIVEDAKQLSYVSYTEICNMAYQGAKVIHPRAVEIAMQAQIPVRVRSTFSEA 245 +G++TADPRIV+DA+ L V+Y EICNMAYQGAKV+HPRAVEIAM A++P+RVRST+SE+ Sbjct: 184 EGVMTADPRIVKDARHLQTVTYNEICNMAYQGAKVVHPRAVEIAMHAKVPLRVRSTYSES 243 Query: 246 EGTLVTHPEGFSDVSHGVVDRFVTGIAYVSNVTQISVECPEGNGTGVQLQIFKSMADNGI 305 EGTL+ +G + V +R VTGIA+VSNVTQI V E +Q +FK MA+ GI Sbjct: 244 EGTLIAAYDGATK-GQDVEERPVTGIAHVSNVTQIKVLAKE-TAYDIQQHVFKEMANEGI 301 Query: 306 SVDFINVTPTEALYTVNDDKSEQAIAALQELGLRPSSLSGCAKXXXXXXXXXXXXXXMAR 365 SVD IN++PT YTVND+ S +A+ L++LG P CAK A+ Sbjct: 302 SVDLINISPTGVAYTVNDNVSARAVEVLKQLGYEPIVTEHCAKVSIVGAGMAGIPGVTAK 361 Query: 366 IVEALSSQNIQILQSADSNTTIWVLVKKEDMVQSLRALHAKFELHR 411 IV AL+ + IQILQSADS+TTIWVLVK+ D+V+++ ALH+ FEL + Sbjct: 362 IVTALAEKGIQILQSADSHTTIWVLVKEIDLVEAVNALHSAFELSK 407 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,216,965,446 Number of extensions: 87454709 Number of successful extensions: 240554 Number of sequences better than 1.0e-05: 2604 Number of HSP's gapped: 237829 Number of HSP's successfully gapped: 2604 Length of query: 411 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 273 Effective length of database: 1,760,596,133 Effective search space: 480642744309 Effective search space used: 480642744309 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)