BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0021.p (287 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIJ5.1 B3KIJ5_9BACI Spore cortex-lytic enzyme 348 4e-94 B1DLG1.1 B1DLG1_9BACL Spore cortex-lytic enzyme 340 1e-91 C0ZBS4.1 C0ZBS4_BREBN Probable spore cortex-lytic enzyme EC=3.5.... 271 4e-71 C2XV01.1 C2XV01_BACCE Spore cortex-lytic enzyme 256 2e-66 C2PWF1.1 C2PWF1_BACCE Spore cortex-lytic enzyme 256 2e-66 >B3KIJ5.1 B3KIJ5_9BACI Spore cortex-lytic enzyme Length = 270 Score = 348 bits (892), Expect = 4e-94, Method: Compositional matrix adjust. Identities = 168/237 (70%), Positives = 189/237 (79%), Gaps = 1/237 (0%) Query: 51 EEALPVFGTTPLKVGSSGQDVYELQGRLKHLGYYAGAIDSQFGAKTRNAVTWFQWKFGME 110 +EALP F +K GS+G+DVYELQGRLK+LG+Y G IDS FG+KT+ AV WFQ +FGM+ Sbjct: 35 QEALPTFSQQTIKFGSTGEDVYELQGRLKYLGFYHGKIDSVFGSKTQGAVKWFQSEFGMK 94 Query: 111 ADGIVGAKTKLKLYNATKAWRPTEPSTSTANKTEXXXXXXXXXXXXXXXXELSSGNTMGL 170 DGIVG K KLKLYNATK +RPT P L+S NTMGL Sbjct: 95 VDGIVGPKVKLKLYNATKDFRPTAPKLHEGGGAAAGAGGNGGGTNQGNN-NLASSNTMGL 153 Query: 171 SENDLRIMANAVYGESRGEPFEGQVAVAAVILNRVKSPNFPNTPSGVIFQPGAFTAVADG 230 S+NDLRIMANAVYGE+RGEPFEGQVAVAAVILNRVKSP+FPNTP GVIF+P AFTAVADG Sbjct: 154 SDNDLRIMANAVYGEARGEPFEGQVAVAAVILNRVKSPSFPNTPHGVIFEPRAFTAVADG 213 Query: 231 QIYLEPNEQARKAVQQALNGWDPSGGCIYYFNPKTATSKWIWSRPQVKTIGQHIFCM 287 QI+LEPN QARKAV+QALNGWDP+GGC+YYFNPKTATS WIW+RPQVKTIGQHIFCM Sbjct: 214 QIWLEPNAQARKAVEQALNGWDPTGGCLYYFNPKTATSPWIWTRPQVKTIGQHIFCM 270 >B1DLG1.1 B1DLG1_9BACL Spore cortex-lytic enzyme Length = 264 Score = 340 bits (872), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 162/238 (68%), Positives = 184/238 (77%) Query: 50 EEEALPVFGTTPLKVGSSGQDVYELQGRLKHLGYYAGAIDSQFGAKTRNAVTWFQWKFGM 109 +++ F L VGSSG DVYELQGRLK+LGYY GAID FG KT+NAVTWFQWKFGM Sbjct: 27 QDDVKETFSNATLTVGSSGSDVYELQGRLKYLGYYNGAIDGSFGTKTKNAVTWFQWKFGM 86 Query: 110 EADGIVGAKTKLKLYNATKAWRPTEPSTSTANKTEXXXXXXXXXXXXXXXXELSSGNTMG 169 ++DG+VGAKTKLKL+ ATK W+ + T S N +G Sbjct: 87 KSDGVVGAKTKLKLWEATKNWQSSAADTGNTTGGSDNSNNSGSNSGSSGGSTPSKSNNLG 146 Query: 170 LSENDLRIMANAVYGESRGEPFEGQVAVAAVILNRVKSPNFPNTPSGVIFQPGAFTAVAD 229 LSE+DL+IMANAVYGESRGEP+EGQVAVAAVILNR+KSP+FPNT SGVIFQP AFTAVAD Sbjct: 147 LSESDLKIMANAVYGESRGEPYEGQVAVAAVILNRLKSPSFPNTISGVIFQPLAFTAVAD 206 Query: 230 GQIYLEPNEQARKAVQQALNGWDPSGGCIYYFNPKTATSKWIWSRPQVKTIGQHIFCM 287 GQI+L PNE ARKAVQ A+NGWDP+GGC YYFNP+TATSKWIWSRPQVKTIG+HIFCM Sbjct: 207 GQIWLTPNETARKAVQDAINGWDPTGGCTYYFNPETATSKWIWSRPQVKTIGKHIFCM 264 >C0ZBS4.1 C0ZBS4_BREBN Probable spore cortex-lytic enzyme EC=3.5.1.28 Length = 261 Score = 271 bits (694), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 133/230 (57%), Positives = 164/230 (71%), Gaps = 1/230 (0%) Query: 57 FGTTPLKVGSSGQDVYELQGRLKHLGYYAGAIDSQFGAKTRNAVTWFQWKFGMEADGIVG 116 F +KVG G DV E+Q RLKHLG+Y G +D FG ++ A+ FQ++FG+ DG++G Sbjct: 32 FSEQIVKVGEEGSDVREMQYRLKHLGFYTGKVDGVFGWRSYWALRNFQYEFGLPIDGVLG 91 Query: 117 AKTKLKLYNATKAWRPTEPSTSTANKTEXXXXXXXXXXXXXXXXELSSGNTMGLSENDLR 176 A+TK+KLYNATK ++PT + + + G+SENDLR Sbjct: 92 AQTKVKLYNATKNYKPTASDLGVPQTATPAPSTPSTPSTPSAPSK-PTYHVAGISENDLR 150 Query: 177 IMANAVYGESRGEPFEGQVAVAAVILNRVKSPNFPNTPSGVIFQPGAFTAVADGQIYLEP 236 +MANAVYGESRGEP+ GQVAVAAVILNR K+ +FPNTP+GVIF+P AFTAVADGQI+L P Sbjct: 151 LMANAVYGESRGEPYIGQVAVAAVILNRTKNASFPNTPAGVIFEPRAFTAVADGQIWLTP 210 Query: 237 NEQARKAVQQALNGWDPSGGCIYYFNPKTATSKWIWSRPQVKTIGQHIFC 286 NEQA+KAV AL GWDP+ G IYYFNP TATS WIWSRPQ+K IG+HIFC Sbjct: 211 NEQAKKAVNDALKGWDPTDGAIYYFNPDTATSGWIWSRPQIKKIGRHIFC 260 >C2XV01.1 C2XV01_BACCE Spore cortex-lytic enzyme Length = 259 Score = 256 bits (654), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 136/248 (54%), Positives = 166/248 (66%), Gaps = 10/248 (4%) Query: 41 SVMTEGIVPEEEALPVFGTTPLKVGSSGQDVYELQGRLKHLGYYAGAIDSQFGAKTRNAV 100 S+M I + + + F ++ G+SG+DV ELQ RLK+ G+Y G +D FG T A+ Sbjct: 19 SLMVSSI--QTKNVEAFSNQVIQRGASGEDVIELQTRLKYNGFYTGKVDGVFGWGTYWAL 76 Query: 101 TWFQWKFGMEADGIVGAKTKLKLYNATKAWRPTEPSTSTANKTEXXXXXXXXXXXXXXXX 160 FQ KFG+ DG+ GAKTK L ATK STANK Sbjct: 77 RNFQQKFGLPVDGLAGAKTKQMLVKATKY------DKSTANKDTTTNKGNSGGGTQENKP 130 Query: 161 ELSSGNTM--GLSENDLRIMANAVYGESRGEPFEGQVAVAAVILNRVKSPNFPNTPSGVI 218 S+G + G S+ND+++MANAVYGESRGEP+ GQVAVAAVILNRV S +FPNT SGVI Sbjct: 131 PKSTGTNVPNGYSQNDIQLMANAVYGESRGEPYLGQVAVAAVILNRVTSASFPNTVSGVI 190 Query: 219 FQPGAFTAVADGQIYLEPNEQARKAVQQALNGWDPSGGCIYYFNPKTATSKWIWSRPQVK 278 F+P AFTAVADGQIYL PNE A+KAV A+NGWDP+G +YYFNP TATSKWIW+RPQ+K Sbjct: 191 FEPRAFTAVADGQIYLTPNENAKKAVLDAINGWDPTGNALYYFNPDTATSKWIWTRPQIK 250 Query: 279 TIGQHIFC 286 IG+HIFC Sbjct: 251 KIGKHIFC 258 >C2PWF1.1 C2PWF1_BACCE Spore cortex-lytic enzyme Length = 259 Score = 256 bits (654), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 136/248 (54%), Positives = 166/248 (66%), Gaps = 10/248 (4%) Query: 41 SVMTEGIVPEEEALPVFGTTPLKVGSSGQDVYELQGRLKHLGYYAGAIDSQFGAKTRNAV 100 S+M I + + + F ++ G+SG+DV ELQ RLK+ G+Y G +D FG T A+ Sbjct: 19 SLMVSSI--QTKNVEAFSNQVIQRGASGEDVIELQTRLKYNGFYTGKVDGVFGWGTYWAL 76 Query: 101 TWFQWKFGMEADGIVGAKTKLKLYNATKAWRPTEPSTSTANKTEXXXXXXXXXXXXXXXX 160 FQ KFG+ DG+ GAKTK L ATK STANK Sbjct: 77 RNFQQKFGLPVDGLAGAKTKQMLVKATKY------DKSTANKDTTTNKGNSGGGTQENKP 130 Query: 161 ELSSGNTM--GLSENDLRIMANAVYGESRGEPFEGQVAVAAVILNRVKSPNFPNTPSGVI 218 S+G + G S+ND+++MANAVYGESRGEP+ GQVAVAAVILNRV S +FPNT SGVI Sbjct: 131 PKSTGTNVPNGYSQNDIQLMANAVYGESRGEPYLGQVAVAAVILNRVTSASFPNTVSGVI 190 Query: 219 FQPGAFTAVADGQIYLEPNEQARKAVQQALNGWDPSGGCIYYFNPKTATSKWIWSRPQVK 278 F+P AFTAVADGQIYL PNE A+KAV A+NGWDP+G +YYFNP TATSKWIW+RPQ+K Sbjct: 191 FEPRAFTAVADGQIYLTPNENAKKAVLDAINGWDPTGNALYYFNPDTATSKWIWTRPQIK 250 Query: 279 TIGQHIFC 286 IG+HIFC Sbjct: 251 KIGKHIFC 258 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.131 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,630,685,141 Number of extensions: 62105165 Number of successful extensions: 94531 Number of sequences better than 1.0e-05: 552 Number of HSP's gapped: 94475 Number of HSP's successfully gapped: 589 Length of query: 287 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 153 Effective length of database: 1,798,280,193 Effective search space: 275136869529 Effective search space used: 275136869529 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 130 (54.7 bits)