BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0019.p (434 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIJ7.1 B3KIJ7_9BACI Peptidase M16 domain protein 682 0.0 B1DLF9.1 B1DLF9_9BACL Peptidase M16 domain protein 578 e-163 C0ZF23.1 C0ZF23_BREBN Peptidase M16 family protein 468 e-130 C2YV71.1 C2YV71_BACCE Putative uncharacterized protein 443 e-122 C1EP10.1 C1EP10_BACC3 Zinc protease, insulinase family 443 e-122 >B3KIJ7.1 B3KIJ7_9BACI Peptidase M16 domain protein Length = 426 Score = 682 bits (1759), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/434 (75%), Positives = 361/434 (83%), Gaps = 8/434 (1%) Query: 1 VEKIHYEKLQETLYHEVMDNGLQVYVLPKPAFLKTYATFATKYGSVDNHFKVAGGEETTV 60 +E IHY+ LQETLY+EVMDNGL VYVLPKP F KTYATFATKYGSVDNHFKV G ET V Sbjct: 1 MESIHYDNLQETLYYEVMDNGLHVYVLPKPGFQKTYATFATKYGSVDNHFKVQGESETRV 60 Query: 61 PDGIAHFLEHKMFEEPEGDIFATFASNGASANAFTSFDQTVYLFSATENIETNLSTLVDF 120 PDGIAHFLEHKMFEEPEGDIFA FASNGASANAFTSFDQTVYLFSATENI NL TL+DF Sbjct: 61 PDGIAHFLEHKMFEEPEGDIFAKFASNGASANAFTSFDQTVYLFSATENIHENLETLIDF 120 Query: 121 VQRPYFTDENVEKEKGIIGQEINMYADNPDWRVYFGLIEAMYSKHPVHIDIAGTIESIAT 180 VQ PYFTD+NVEKEKGIIGQEINMY DNPDWRVYFGLIEAMY HPVHIDIAGT+ESI T Sbjct: 121 VQNPYFTDQNVEKEKGIIGQEINMYQDNPDWRVYFGLIEAMYKVHPVHIDIAGTVESIGT 180 Query: 181 ITKETLYTCYNSFYHPSNMLLFVVGGVDPEKVFELIRNNQNGKSYGKQGEXXXXKQGEIQ 240 ITKE LYTCYN+FYHPSNMLLFVVGGVDPE+ LIR+NQ KSY KQG I+ Sbjct: 181 ITKEDLYTCYNAFYHPSNMLLFVVGGVDPEETMNLIRSNQARKSYD--------KQGSIE 232 Query: 241 RIFEEEPVQVAAKVKESKLAVSMPKMLFGFKEKVEGLTGEAAVRRXXXXXXXXXXXXGSS 300 R+F+ EP V K +ES+LAVS+PK LFGFKEK GL+ + +RR GSS Sbjct: 233 RLFDPEPQGVEEKRRESRLAVSLPKCLFGFKEKQVGLSADEQLRRDLTTKLMMDLLFGSS 292 Query: 301 TALYQKLYDEELISDSFGHEFNSSPQYAFSAIGGDTKDTDLLLKRIKEEINLVLASGFAE 360 T LYQKLYDE+LISDSFGHE+NSSPQYAFSA+GGDTKD D LL+RI+ E++ + A+GF Sbjct: 293 TELYQKLYDEDLISDSFGHEYNSSPQYAFSAVGGDTKDPDQLLERIRTEVDKLKAAGFQA 352 Query: 361 KDFERARKKKIGGYLRMLNSPESIAHEFTRYQFRGGDLFEVLPLYESITLEEVNERLRSH 420 DFERARKKK+GGYLRMLNSPE+IAHEFTRYQFRG D F VLP+YESITLE+VN RL+ H Sbjct: 353 SDFERARKKKMGGYLRMLNSPENIAHEFTRYQFRGADFFNVLPVYESITLEDVNRRLQEH 412 Query: 421 VDWEQLAVSLVVSP 434 VDW QLAVS+VVSP Sbjct: 413 VDWNQLAVSIVVSP 426 >B1DLF9.1 B1DLF9_9BACL Peptidase M16 domain protein Length = 431 Score = 578 bits (1491), Expect = e-163, Method: Compositional matrix adjust. Identities = 283/434 (65%), Positives = 330/434 (76%), Gaps = 8/434 (1%) Query: 1 VEKIHYEKLQETLYHEVMDNGLQVYVLPKPAFLKTYATFATKYGSVDNHFKVAGGEETTV 60 +E + Y+ +QETLY EV+DNGL+V VLPK F KTYATFATKYGSVDN F V E V Sbjct: 1 METLDYKGVQETLYREVLDNGLEVIVLPKEGFQKTYATFATKYGSVDNKFAVGDQEPVRV 60 Query: 61 PDGIAHFLEHKMFEEPEGDIFATFASNGASANAFTSFDQTVYLFSATENIETNLSTLVDF 120 PDGIAHFLEHKMFEEP GDIFATFAS GASANAFTSFD+TVYLFSATE I NL TL+DF Sbjct: 61 PDGIAHFLEHKMFEEPTGDIFATFASQGASANAFTSFDRTVYLFSATEQIPANLETLIDF 120 Query: 121 VQRPYFTDENVEKEKGIIGQEINMYADNPDWRVYFGLIEAMYSKHPVHIDIAGTIESIAT 180 VQ PYFTD+NV+KEKGII QEINMY DNPDWRVYFGLI+AMY HP+HIDIAGT+ESI Sbjct: 121 VQHPYFTDQNVDKEKGIIAQEINMYKDNPDWRVYFGLIDAMYHTHPIHIDIAGTVESIYQ 180 Query: 181 ITKETLYTCYNSFYHPSNMLLFVVGGVDPEKVFELIRNNQNGKSYGKQGEXXXXKQGEIQ 240 I KETLY CY +FYHPSNMLLFVVGGV E+VFEL+RNNQ KS+ QGEI+ Sbjct: 181 IDKETLYRCYETFYHPSNMLLFVVGGVKAEEVFELVRNNQARKSF--------KPQGEIK 232 Query: 241 RIFEEEPVQVAAKVKESKLAVSMPKMLFGFKEKVEGLTGEAAVRRXXXXXXXXXXXXGSS 300 R FE EP V K +KL VS+PK++FGFKEK GLTGEA + G+S Sbjct: 233 RFFEHEPESVKVPRKVTKLPVSLPKVMFGFKEKKTGLTGEALLSHEVTSKLMLDALFGAS 292 Query: 301 TALYQKLYDEELISDSFGHEFNSSPQYAFSAIGGDTKDTDLLLKRIKEEINLVLASGFAE 360 +ALYQ LY++ LISDSFG EFN S YAFS IGGD+ + D LL RI+ + L +G Sbjct: 293 SALYQDLYEQNLISDSFGTEFNVSTDYAFSVIGGDSPNPDELLSRIRTAVEEALRTGIEP 352 Query: 361 KDFERARKKKIGGYLRMLNSPESIAHEFTRYQFRGGDLFEVLPLYESITLEEVNERLRSH 420 FER+++KKIGGYLRMLNSPE+IA EFTRY+FR D+F++L LYE+ TLE+VNER+R H Sbjct: 353 AAFERSKRKKIGGYLRMLNSPEAIAGEFTRYRFRDSDMFDLLSLYENCTLEQVNERIREH 412 Query: 421 VDWEQLAVSLVVSP 434 D++QLA+S+V P Sbjct: 413 FDFQQLAISIVEKP 426 >C0ZF23.1 C0ZF23_BREBN Peptidase M16 family protein Length = 430 Score = 468 bits (1205), Expect = e-130, Method: Compositional matrix adjust. Identities = 229/428 (53%), Positives = 307/428 (71%), Gaps = 8/428 (1%) Query: 6 YEKLQETLYHEVMDNGLQVYVLPKPAFLKTYATFATKYGSVDNHFKVAGGEETTVPDGIA 65 +E++ ET+YHE + NGLQVY++PK F KTYA F T+YGS+D+HF+ GEE VPDGIA Sbjct: 6 FEQVNETVYHEALQNGLQVYLVPKQGFSKTYAVFTTRYGSIDSHFRTRSGEEINVPDGIA 65 Query: 66 HFLEHKMFEEPEGDIFATFASNGASANAFTSFDQTVYLFSATENIETNLSTLVDFVQRPY 125 HFLEHKMFE+ E D+ F+ NGAS NAFTSF++T YLFS T+ ++ NL+ L+D+VQ PY Sbjct: 66 HFLEHKMFEKKERDVMHEFSKNGASCNAFTSFNRTAYLFSCTDKLDDNLNLLLDYVQDPY 125 Query: 126 FTDENVEKEKGIIGQEINMYADNPDWRVYFGLIEAMYSKHPVHIDIAGTIESIATITKET 185 FTD +VEKEKGIIGQEI MY DNPDW+VY L++AMY K+P++I+IAGTIE+I+ ITKE Sbjct: 126 FTDASVEKEKGIIGQEITMYEDNPDWKVYMNLLKAMYQKYPINIEIAGTIETISHITKEN 185 Query: 186 LYTCYNSFYHPSNMLLFVVGGVDPEKVFELIRNNQNGKSYGKQGEXXXXKQGEIQRIFEE 245 LY CY +FYHP+NMLL VVG +PE + +LIR NQ K + +I R+F E Sbjct: 186 LYQCYETFYHPANMLLLVVGSFEPEAIMKLIRENQGAKEFP--------PAPQITRVFPE 237 Query: 246 EPVQVAAKVKESKLAVSMPKMLFGFKEKVEGLTGEAAVRRXXXXXXXXXXXXGSSTALYQ 305 EP + A E+ L V +PK + G KEK GLT EA ++R G+S+A+Y+ Sbjct: 238 EPSEPAEAKVEAFLTVGLPKCMIGIKEKENGLTKEALLKRELTTKLVLDIAFGTSSAVYE 297 Query: 306 KLYDEELISDSFGHEFNSSPQYAFSAIGGDTKDTDLLLKRIKEEINLVLASGFAEKDFER 365 +LYD ELI++SF +++S YA++ IGGDT D + L++ IK EI + +G A++DFER Sbjct: 298 RLYDSELITESFDFDYSSEQDYAYTIIGGDTPDPERLVETIKAEIEQLKQTGIAQEDFER 357 Query: 366 ARKKKIGGYLRMLNSPESIAHEFTRYQFRGGDLFEVLPLYESITLEEVNERLRSHVDWEQ 425 A++KKIG +LR LNS E IA++FT ++F G DLF V+P ESIT E+V +RL+ H EQ Sbjct: 358 AKRKKIGNFLRSLNSVEFIANQFTSFKFNGNDLFSVVPTLESITREDVEKRLKEHFLAEQ 417 Query: 426 LAVSLVVS 433 +AVS+V S Sbjct: 418 MAVSIVRS 425 >C2YV71.1 C2YV71_BACCE Putative uncharacterized protein Length = 428 Score = 443 bits (1140), Expect = e-122, Method: Compositional matrix adjust. Identities = 226/436 (51%), Positives = 296/436 (67%), Gaps = 15/436 (3%) Query: 1 VEKIHYEKLQETLYHEVMDNGLQVYVLPKPAFLKTYATFATKYGSVDNHFKVAGGEETT- 59 +EKI Y++L+ETLY+E + NGL VY+LPK F KT+ATF TKYGSVDN F G EE Sbjct: 1 MEKIVYKQLKETLYYEKLPNGLDVYILPKQGFNKTFATFTTKYGSVDNTFVPLGKEEMVR 60 Query: 60 VPDGIAHFLEHKMFEEPEGDIFATFASNGASANAFTSFDQTVYLFSATENIETNLSTLVD 119 VPDGIAHFLEHK+FE+ + D F F+ GASANAFTSF +T YLFS T N+E NL+TL++ Sbjct: 61 VPDGIAHFLEHKLFEKEDHDAFQLFSKQGASANAFTSFTRTAYLFSCTSNVEQNLNTLLN 120 Query: 120 FVQRPYFTDENVEKEKGIIGQEINMYADNPDWRVYFGLIEAMYSKHPVHIDIAGTIESIA 179 FVQ PYF+++ VEKEKGIIGQEI MY DNPDWR+YFGLI++++ KHP+ IDIAGTIESI+ Sbjct: 121 FVQEPYFSEKTVEKEKGIIGQEIQMYQDNPDWRLYFGLIDSLFVKHPIKIDIAGTIESIS 180 Query: 180 TITKETLYTCYNSFYHPSNMLLFVVGGVDPEKVFELIRNNQNGKSYGKQGEXXXXKQGEI 239 ITK+ LY CY +FYHPSNMLLFVVG +DPEK +L+R NQ K Y Q EI Sbjct: 181 KITKDLLYECYETFYHPSNMLLFVVGAIDPEKTMDLVRENQAKKDY--------KNQPEI 232 Query: 240 QRIFEEEPVQVAAKVKESKLAVSMPKMLFGFKEKVEGLTGEAAVRRXXXXXXXXXXXXGS 299 R FEEEP +V K K + V PK L G K GEA +++ G Sbjct: 233 VRSFEEEPDEVNEKKKIISMPVQTPKCLVGIKATNLKEKGEALLKQEIALTLLLDYLFGK 292 Query: 300 STALYQKLYDEELISDSFGHEFNSSPQYAFSAIGGDTKDTDLLLKRIKEEINLVLASGFA 359 S+ Y+ LY+E LI DSF +++ + F+ +GGDTK D L +R+K +++L + + Sbjct: 293 SSVHYESLYNEGLIDDSFSYDYTEENNFGFAMVGGDTKQPDELEERLK---DILLNTNYD 349 Query: 360 EKD---FERARKKKIGGYLRMLNSPESIAHEFTRYQFRGGDLFEVLPLYESITLEEVNER 416 + D ER +KKKIGG+LR LNSPE IA++FTRY F LFE L + ES+T++++ E Sbjct: 350 QLDELALERVKKKKIGGFLRSLNSPEYIANQFTRYAFNESSLFEALTVLESLTVQDLQEV 409 Query: 417 LRSHVDWEQLAVSLVV 432 + + E+++V V+ Sbjct: 410 AKLLLSEEKMSVCQVL 425 >C1EP10.1 C1EP10_BACC3 Zinc protease, insulinase family Length = 428 Score = 443 bits (1139), Expect = e-122, Method: Compositional matrix adjust. Identities = 226/436 (51%), Positives = 296/436 (67%), Gaps = 15/436 (3%) Query: 1 VEKIHYEKLQETLYHEVMDNGLQVYVLPKPAFLKTYATFATKYGSVDNHFKVAGGEETT- 59 +EKI YE+L+ETLY+E + NGL VY+LPK F KT+ATF TKYGSVDN F G EE Sbjct: 1 MEKIVYEQLKETLYYEKLPNGLDVYILPKQGFNKTFATFTTKYGSVDNTFVPLGKEEMIR 60 Query: 60 VPDGIAHFLEHKMFEEPEGDIFATFASNGASANAFTSFDQTVYLFSATENIETNLSTLVD 119 VPDGIAHFLEHK+FE+ + D F F+ GASANAFTSF +T YLFS T N+E NL+TL++ Sbjct: 61 VPDGIAHFLEHKLFEKEDHDAFQLFSKQGASANAFTSFTRTAYLFSCTSNVEQNLNTLLN 120 Query: 120 FVQRPYFTDENVEKEKGIIGQEINMYADNPDWRVYFGLIEAMYSKHPVHIDIAGTIESIA 179 FVQ PYF+++ VEKEKGIIGQEI MY DNPDWR+YFGLI++++ KHP+ IDIAGTIESI+ Sbjct: 121 FVQEPYFSEKTVEKEKGIIGQEIQMYQDNPDWRLYFGLIDSLFVKHPIKIDIAGTIESIS 180 Query: 180 TITKETLYTCYNSFYHPSNMLLFVVGGVDPEKVFELIRNNQNGKSYGKQGEXXXXKQGEI 239 ITK+ LY CY +FYHPSNMLLFVVG +DPEK +L+R NQ K Y Q EI Sbjct: 181 KITKDLLYECYETFYHPSNMLLFVVGAIDPEKTMDLVRENQAKKDY--------KNQPEI 232 Query: 240 QRIFEEEPVQVAAKVKESKLAVSMPKMLFGFKEKVEGLTGEAAVRRXXXXXXXXXXXXGS 299 R FEEEP +V K K + V PK L G K GEA +++ G Sbjct: 233 VRSFEEEPDEVNEKKKIISMPVQTPKCLVGIKATKLKEKGEALLKQEIALTLLLDYLFGK 292 Query: 300 STALYQKLYDEELISDSFGHEFNSSPQYAFSAIGGDTKDTDLLLKRIKEEINLVLASGFA 359 S+ Y+ LY+E LI DSF +++ + F+ +GGDTK D L +R+K +++L + + Sbjct: 293 SSVHYESLYNEGLIDDSFSYDYTEENNFGFAMVGGDTKQPDELEERLK---SILLNTNYN 349 Query: 360 EKD---FERARKKKIGGYLRMLNSPESIAHEFTRYQFRGGDLFEVLPLYESITLEEVNER 416 + D ER +KKKIGG+LR LNSPE IA++FTRY F LF+ L + ES+T++++ E Sbjct: 350 QLDEAALERVKKKKIGGFLRSLNSPEYIANQFTRYAFNESSLFDALTVLESLTVQDLQEV 409 Query: 417 LRSHVDWEQLAVSLVV 432 + + E+++V V+ Sbjct: 410 AQLLLSEEKMSVCQVL 425 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,500,189,472 Number of extensions: 102701228 Number of successful extensions: 245434 Number of sequences better than 1.0e-05: 2097 Number of HSP's gapped: 243365 Number of HSP's successfully gapped: 2100 Length of query: 434 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 295 Effective length of database: 1,751,175,118 Effective search space: 516596659810 Effective search space used: 516596659810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)