BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0004.p (316 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIL1.1 B3KIL1_9BACI Peptidase M19 renal dipeptidase 405 e-111 B1DLE5.1 B1DLE5_9BACL Peptidase M19 renal dipeptidase 299 2e-79 Q9KH70.1 Q9KH70_9BACL Thermostable dipeptidase Bdp 246 2e-63 A6CSM2.1 A6CSM2_9BACI Microsomal dipeptidase 230 1e-58 Q71WW4.1 Q71WW4_LISMF Renal dipeptidase family protein 229 2e-58 >B3KIL1.1 B3KIL1_9BACI Peptidase M19 renal dipeptidase Length = 323 Score = 405 bits (1041), Expect = e-111, Method: Compositional matrix adjust. Identities = 193/315 (61%), Positives = 238/315 (75%), Gaps = 2/315 (0%) Query: 1 VADFHCDVLSKLQASPDIDFRKDSRLDVTLDRLLAGGVELQVFAIYLSTRREKASFERIL 60 DFHCDVLSK+ P F LDVT+ RL G + LQVFA++LS + + SFER+L Sbjct: 3 TVDFHCDVLSKMWEDPKASFVDGPHLDVTMQRLEHGDLALQVFAVFLSEKFGRPSFERVL 62 Query: 61 GQIESFRNQVAGSGGLQWLCWKEEAAEQINTPTGR-GMLSLEGVDGLEGNLFYAQLCFEL 119 Q++ +R+++ +G L+ L W+E+ E T R G+LSLEGVDGLEGNL+Y QL F++ Sbjct: 63 AQLDVYRSRIVNAGHLKPLLWREQVREMRQRKTERYGVLSLEGVDGLEGNLYYVQLLFDM 122 Query: 120 GVRFMGVTWNYANWAADGVLEQRNGGFTEQGRKLINWCNESGMLLDVSHLAPAGFWELAE 179 GVR MGVTWNYANWAADGVLEQRNGGFTE+GR+LI C+ +G++LDVSHL+ AGFWELAE Sbjct: 123 GVRLMGVTWNYANWAADGVLEQRNGGFTERGRQLIEQCHRTGIILDVSHLSQAGFWELAE 182 Query: 180 RSVRPFIASHSNAAAVCGHPRNLSDEQIQALIAMDGRIGVTFVPWFVRDGGE-AKAEDVL 238 + RP IASHSNA ++C HPRNLSDEQI+ALIA++G IG+TFVPWF+R G + EDVL Sbjct: 183 LTQRPVIASHSNAYSICPHPRNLSDEQIRALIALNGMIGITFVPWFIRSGVQRVTPEDVL 242 Query: 239 RHIEHICSLGGSGQIMFGSDFDGIDCWVEGLEHPGKYAEFAELLLARYPEDAVKGWLHGN 298 HIE IC LGG IMFGSDFDGID W+EGLEHPG+Y +F ELLL YPE VKGW+ N Sbjct: 243 PHIEKICELGGEKHIMFGSDFDGIDQWIEGLEHPGRYPDFQELLLKFYPESTVKGWMSDN 302 Query: 299 ALSFLAKNLPSKTIQ 313 L FL ++LPS +Q Sbjct: 303 PLRFLEQHLPSSPVQ 317 >B1DLE5.1 B1DLE5_9BACL Peptidase M19 renal dipeptidase Length = 314 Score = 299 bits (766), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 150/310 (48%), Positives = 205/310 (66%), Gaps = 3/310 (0%) Query: 1 VADFHCDVLSKLQASPDIDFRKDSR--LDVTLDRLLAGGVELQVFAIYLSTRREKASFER 58 +ADFHCDVLSKL P++ F++ LDVT +RL G LQ FA+Y+ + A E Sbjct: 5 IADFHCDVLSKLLVHPNLTFQESEPGLLDVTYERLKESGAVLQTFAVYIPEKLNGA-IEP 63 Query: 59 ILGQIESFRNQVAGSGGLQWLCWKEEAAEQINTPTGRGMLSLEGVDGLEGNLFYAQLCFE 118 IL I+ F +V ++ + + + E + +LSLEGVDGL+G++ +L Sbjct: 64 ILECIDHFYEKVLACPDMKLIRNQSDLKESLAAGKIGALLSLEGVDGLQGSIPILRLLHR 123 Query: 119 LGVRFMGVTWNYANWAADGVLEQRNGGFTEQGRKLINWCNESGMLLDVSHLAPAGFWELA 178 LGVR G+TWN+ANWAADGV+E R GG T +G + + C++ G+L+DVSHL+ FW++A Sbjct: 124 LGVRAAGLTWNHANWAADGVMEPRGGGLTAKGNEFVAECDKLGILVDVSHLSEKAFWDVA 183 Query: 179 ERSVRPFIASHSNAAAVCGHPRNLSDEQIQALIAMDGRIGVTFVPWFVRDGGEAKAEDVL 238 + + R IASHSNA A+ HPRNL+D+QI+A+IA+ G IG+T+VPWFV +G + ++VL Sbjct: 184 DLAARTIIASHSNAKAILNHPRNLTDDQIKAIIALQGLIGITYVPWFVAEGEQVSVDNVL 243 Query: 239 RHIEHICSLGGSGQIMFGSDFDGIDCWVEGLEHPGKYAEFAELLLARYPEDAVKGWLHGN 298 RHI+HI LGG IM GSDFDGID +V GL HPG+ E LL Y E+ K +L GN Sbjct: 244 RHIDHIGGLGGEQHIMLGSDFDGIDKYVNGLTHPGEVYRLREALLKNYSEEQTKRFLSGN 303 Query: 299 ALSFLAKNLP 308 ALSFL +NLP Sbjct: 304 ALSFLNENLP 313 >Q9KH70.1 Q9KH70_9BACL Thermostable dipeptidase Bdp Length = 307 Score = 246 bits (627), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 133/304 (43%), Positives = 187/304 (61%), Gaps = 8/304 (2%) Query: 1 VADFHCDVLSKLQASPD-IDFRKDSRLDVTLDRLLAGGVELQVFAIYLSTR-REKASFER 58 + DFHCD L K+ S + + FR + L L+RL G V+LQ FA+Y+ + + + F+ Sbjct: 3 IIDFHCDALYKMWKSKEPLSFRDSAELHTNLERLKQGRVKLQCFALYVPPQLKSEQQFQA 62 Query: 59 ILGQIESFRNQVAGSGGLQWLCWKEEAAEQINTPTGR--GMLSLEGVDGLEGNLFYAQLC 116 +L QI+ F +V ++ + +E A+ G ML+LEGVD + +L ++ Sbjct: 63 VLDQIDYFYEEVLKQPEMKHV---KEWADLDTLGDGEIGAMLTLEGVDAIGNDLKKLRIL 119 Query: 117 FELGVRFMGVTWNYANWAADGVLEQRNGGFTEQGRKLINWCNESGMLLDVSHLAPAGFWE 176 F+LGVR +G+TWN+AN AADG LE+RN G T G++++ NE +L DVSHL FW+ Sbjct: 120 FQLGVRSVGLTWNFANLAADGALEKRNAGLTAFGKEIVRLNNEHKVLTDVSHLCEQSFWD 179 Query: 177 LAERSVRPFIASHSNAAAVCGHPRNLSDEQIQALIAMDGRIGVTFVPWFVRDGGEAKAED 236 + E + P IASHSNA A+C HPRNL DEQ +A+ G I + + P FV+ GG+A +D Sbjct: 180 VMELADYP-IASHSNARAICDHPRNLYDEQAKAMFDKGGMIHIVYYPHFVKKGGQATIDD 238 Query: 237 VLRHIEHICSLGGSGQIMFGSDFDGIDCWVEGLEHPGKYAEFAELLLARYPEDAVKGWLH 296 ++RHI+H CSLGG QI GSDFDGI+ +VEGLEH G LL RY E V+G+ Sbjct: 239 LIRHIDHFCSLGGVRQIGLGSDFDGIESFVEGLEHAGMIDNLLNELLKRYSESEVRGFAS 298 Query: 297 GNAL 300 N L Sbjct: 299 ENFL 302 >A6CSM2.1 A6CSM2_9BACI Microsomal dipeptidase Length = 307 Score = 230 bits (587), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 128/301 (42%), Positives = 177/301 (58%), Gaps = 5/301 (1%) Query: 1 VADFHCDVLSKLQA-SPDIDFRKDSRLDVTLDRLLAGGVELQVFAIYL-STRREKASFER 58 + D HCDVL KL D+ F+ S L +T +L G ++Q+FAIYL S + F+ Sbjct: 2 IFDGHCDVLLKLHHYKEDVSFQSQSDLHITYPQLRQAGSKVQLFAIYLPSYLKPGQRFQT 61 Query: 59 ILGQIESFRNQV-AGSGGLQWLCWKEEAAEQINTPTGRGMLSLEGVDGLEGNLFYAQLCF 117 L +E F ++ + ++ + K++ A+ G ML+LEG + +E +L + Sbjct: 62 ALEMVEVFHKKILEPNPKMKMVHSKKDIADLKEDEIG-AMLTLEGCEAIEEDLVKLHTLY 120 Query: 118 ELGVRFMGVTWNYANWAADGVLEQRNGGFTEQGRKLINWCNESGMLLDVSHLAPAGFWEL 177 +LGVR +G+TWN+AN ADG LE R G T+ GR+ + + N+ +L DVSHL FW+ Sbjct: 121 QLGVRAVGLTWNWANAVADGALEPRAAGLTKFGRETVEFLNDHKILTDVSHLCERSFWDT 180 Query: 178 AERSVRPFIASHSNAAAVCGHPRNLSDEQIQALIAMDGRIGVTFVPWFVRDGGEAKAEDV 237 E + P IASHSN CGHPRNL D+QI+ALI DG +G+TFVP F+ GGEA D+ Sbjct: 181 IEITKYP-IASHSNTFRHCGHPRNLKDDQIKALIKKDGVMGLTFVPPFLNQGGEADVSDI 239 Query: 238 LRHIEHICSLGGSGQIMFGSDFDGIDCWVEGLEHPGKYAEFAELLLARYPEDAVKGWLHG 297 LRH++H+CSLGG + FGSDFDGI VEGL +Y L Y E VK +L Sbjct: 240 LRHLDHVCSLGGENHVGFGSDFDGISQTVEGLSCYREYDNLVGSLQKNYSESQVKKFLFE 299 Query: 298 N 298 N Sbjct: 300 N 300 >Q71WW4.1 Q71WW4_LISMF Renal dipeptidase family protein Length = 308 Score = 229 bits (585), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 130/307 (42%), Positives = 175/307 (57%), Gaps = 3/307 (0%) Query: 1 VADFHCDVLSKLQASP-DIDFRKDSRLDVTLDRLLAGGVELQVFAIYLSTRRE-KASFER 58 V D HCD L KLQA F+ LDV +RL+ + LQ FAI+L + +++ Sbjct: 3 VIDTHCDALYKLQAGKGKYTFQDAEELDVNFERLIEAKMLLQGFAIFLDEDIPVEHKWKK 62 Query: 59 ILGQIESFRNQVAGSGGLQWLCWKEEAAEQINTPTGRGMLSLEGVDGLEGNLFYAQLCFE 118 + Q+ F+ V GG+ K E + ML+LEG++ + +L + Sbjct: 63 AVEQVNIFKQHVLHKGGIIHHVKKWCDLENLPEDKIGAMLTLEGIEPIGRDLDKLTQLLD 122 Query: 119 LGVRFMGVTWNYANWAADGVLEQRNGGFTEQGRKLINWCNESGMLLDVSHLAPAGFWELA 178 GV +G+TWN AN AADG++E+R G T G+ +I+ NE + DVSHL+ FWE Sbjct: 123 GGVLSVGLTWNNANLAADGIMEERGAGLTRFGKDIIHLLNERKVFTDVSHLSVKAFWETL 182 Query: 179 ERSVRPFIASHSNAAAVCGHPRNLSDEQIQALIAMDGRIGVTFVPWFVRDGGEAKAEDVL 238 E++ IASHSNA A+C HPRNL DEQI+A+I D I V F P F + G A EDV+ Sbjct: 183 EQA-EFVIASHSNAKAICSHPRNLDDEQIKAMIEHDAMIHVVFYPLFTTNNGVADTEDVI 241 Query: 239 RHIEHICSLGGSGQIMFGSDFDGIDCWVEGLEHPGKYAEFAELLLARYPEDAVKGWLHGN 298 RHI+HIC LGG I FGSDFDGI V+GLEH GKY F E L Y ++ ++G+ N Sbjct: 242 RHIDHICELGGLKNIGFGSDFDGIPDHVKGLEHVGKYQSFLETLEKHYTKEEIEGFASRN 301 Query: 299 ALSFLAK 305 L+ L K Sbjct: 302 FLNHLPK 308 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,026,195,362 Number of extensions: 88981342 Number of successful extensions: 173980 Number of sequences better than 1.0e-05: 875 Number of HSP's gapped: 172943 Number of HSP's successfully gapped: 875 Length of query: 316 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 181 Effective length of database: 1,788,859,178 Effective search space: 323783511218 Effective search space used: 323783511218 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)