BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0003.p (190 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIL3.1 B3KIL3_9BACI Propanediol utilization protein 310 5e-83 B1DLE2.1 B1DLE2_9BACL Propanediol utilization protein 255 1e-66 Q5L1D6.1 Q5L1D6_GEOKA Hypothetical conserved protein 214 4e-54 Q2RJ94.1 Q2RJ94_MOOTA Propanediol utilization protein 214 4e-54 B5AGY6.1 B5AGY6_GEOTH Putative uncharacterized protein 214 4e-54 >B3KIL3.1 B3KIL3_9BACI Propanediol utilization protein Length = 190 Score = 310 bits (793), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 147/170 (86%), Positives = 160/170 (94%) Query: 1 MSKTVPVGVSARHIHLTQEHVEALFGPGYQLTEFKPLSQPGQFAANEQVAVIGTKGKFDK 60 MSKTVPVGVSARHIHLTQEH+EALFGPGYQLTEFKPLSQPGQFAANE VAVIGTKG+FDK Sbjct: 1 MSKTVPVGVSARHIHLTQEHIEALFGPGYQLTEFKPLSQPGQFAANETVAVIGTKGQFDK 60 Query: 61 VRILGPVRPASQLEVSRTDAFSLGVKAPVRESGHIEGTPGITIKGPAGELELETGVIVAA 120 VRILGP RPA+QLE+SRTD+F++GVKAPVRESG+I GTPGITIKGPAGE+ + GVIVAA Sbjct: 61 VRILGPARPATQLEISRTDSFAIGVKAPVRESGNIAGTPGITIKGPAGEVTVNEGVIVAA 120 Query: 121 RHIHFHTSEAEAWGIADKQMLKVRLGGERGLILENVIARVSDNFKLDMHI 170 RHIHFHTS+AE WGI DK +LKVRLGGERGL+LENVIARVSDNF LDMHI Sbjct: 121 RHIHFHTSDAEQWGIKDKDLLKVRLGGERGLVLENVIARVSDNFALDMHI 170 >B1DLE2.1 B1DLE2_9BACL Propanediol utilization protein Length = 191 Score = 255 bits (651), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 126/168 (75%), Positives = 142/168 (84%) Query: 3 KTVPVGVSARHIHLTQEHVEALFGPGYQLTEFKPLSQPGQFAANEQVAVIGTKGKFDKVR 62 K VPVGVSARHIHL+ EHV LFG G +LTE K LSQPGQ+AANE VAVIG KG F KVR Sbjct: 4 KLVPVGVSARHIHLSAEHVAILFGKGSELTEAKALSQPGQYAANETVAVIGPKGSFPKVR 63 Query: 63 ILGPVRPASQLEVSRTDAFSLGVKAPVRESGHIEGTPGITIKGPAGELELETGVIVAARH 122 ILGPVR A+QLEVSRTDAF+LG+ PVRESG+I+G+ GIT+KGPAGE+ +E GVIVAARH Sbjct: 64 ILGPVRKATQLEVSRTDAFALGINPPVRESGNIQGSAGITLKGPAGEVTIEEGVIVAARH 123 Query: 123 IHFHTSEAEAWGIADKQMLKVRLGGERGLILENVIARVSDNFKLDMHI 170 IHFHTS+AE WGIADKQ LKVR GERG++ E VIARVS +F LDMHI Sbjct: 124 IHFHTSDAEKWGIADKQHLKVRFQGERGVVFEQVIARVSPDFALDMHI 171 >Q5L1D6.1 Q5L1D6_GEOKA Hypothetical conserved protein Length = 189 Score = 214 bits (544), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 102/166 (61%), Positives = 127/166 (76%) Query: 5 VPVGVSARHIHLTQEHVEALFGPGYQLTEFKPLSQPGQFAANEQVAVIGTKGKFDKVRIL 64 +PVGVS RHIHL++EH+ ALFG G +LT KPLSQPGQFAA E V V G KGK + VR+L Sbjct: 3 IPVGVSNRHIHLSKEHMAALFGEGAELTVLKPLSQPGQFAAKETVTVEGPKGKIENVRVL 62 Query: 65 GPVRPASQLEVSRTDAFSLGVKAPVRESGHIEGTPGITIKGPAGELELETGVIVAARHIH 124 GPVR +QLE+S+TD+F LGV PVR SG I+GTPGITI GP G + ++ GVI+A RHIH Sbjct: 63 GPVRSLTQLEISKTDSFKLGVAPPVRLSGDIDGTPGITIHGPKGTVTVDKGVIIAKRHIH 122 Query: 125 FHTSEAEAWGIADKQMLKVRLGGERGLILENVIARVSDNFKLDMHI 170 +AE +G+ DKQ++KV+ GER LI + VI RVS++F LDMHI Sbjct: 123 MTPKDAETFGVCDKQVVKVKTQGERALIFDEVIVRVSEDFALDMHI 168 >Q2RJ94.1 Q2RJ94_MOOTA Propanediol utilization protein Length = 204 Score = 214 bits (544), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 94/166 (56%), Positives = 130/166 (78%) Query: 5 VPVGVSARHIHLTQEHVEALFGPGYQLTEFKPLSQPGQFAANEQVAVIGTKGKFDKVRIL 64 VPVGVS RH+HLT+EH++ LFGPGY+LT+ + + QPGQFAA E V ++G KG +KVR+L Sbjct: 8 VPVGVSGRHVHLTREHLQTLFGPGYELTKLRDVVQPGQFAAKETVTIVGPKGVLEKVRVL 67 Query: 65 GPVRPASQLEVSRTDAFSLGVKAPVRESGHIEGTPGITIKGPAGELELETGVIVAARHIH 124 GP+R +Q+E+SRTD+F LGV P+R+SG EG+PG + GPAG + L+ GVI+A RHIH Sbjct: 68 GPLRSYTQVEISRTDSFKLGVNPPIRDSGDHEGSPGCVLVGPAGVVTLKQGVIIALRHIH 127 Query: 125 FHTSEAEAWGIADKQMLKVRLGGERGLILENVIARVSDNFKLDMHI 170 HT EA+ +G+ DK ++ V++GGERGLI NV+ RV+ NF+L+ H+ Sbjct: 128 MHTDEAKRYGLKDKDLVTVQVGGERGLIFTNVLVRVNPNFRLEFHV 173 >B5AGY6.1 B5AGY6_GEOTH Putative uncharacterized protein Length = 188 Score = 214 bits (544), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 102/166 (61%), Positives = 127/166 (76%) Query: 5 VPVGVSARHIHLTQEHVEALFGPGYQLTEFKPLSQPGQFAANEQVAVIGTKGKFDKVRIL 64 +PVGVS RHIHL++EH+ ALFG G +LT KPLSQPGQFAA E V V G KGK + VR+L Sbjct: 2 IPVGVSNRHIHLSKEHMAALFGEGAELTVLKPLSQPGQFAAKETVTVEGPKGKIENVRVL 61 Query: 65 GPVRPASQLEVSRTDAFSLGVKAPVRESGHIEGTPGITIKGPAGELELETGVIVAARHIH 124 GPVR +QLE+S+TD+F LGV PVR SG I+GTPGITI GP G + ++ GVI+A RHIH Sbjct: 62 GPVRSLTQLEISKTDSFKLGVAPPVRLSGDIDGTPGITIHGPKGTVTVDKGVIIAKRHIH 121 Query: 125 FHTSEAEAWGIADKQMLKVRLGGERGLILENVIARVSDNFKLDMHI 170 +AE +G+ DKQ++KV+ GER LI + VI RVS++F LDMHI Sbjct: 122 MTPKDAETFGVCDKQVVKVKTQGERALIFDEVIVRVSEDFALDMHI 167 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,012,399,594 Number of extensions: 40396813 Number of successful extensions: 76932 Number of sequences better than 1.0e-05: 253 Number of HSP's gapped: 76612 Number of HSP's successfully gapped: 253 Length of query: 190 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 62 Effective length of database: 1,854,806,283 Effective search space: 114997989546 Effective search space used: 114997989546 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)