BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0001.p (517 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAP4.1 B3KAP4_9BACI DEAD/DEAH box helicase domain protein 623 e-176 A6EEW7.1 A6EEW7_9SPHI Possible ATP-dependent RNA helicase 510 e-142 C4D4I2.1 C4D4I2_9SPHI DNA/RNA helicase, superfamily II 508 e-142 C1YXN6.1 C1YXN6_PEDHE DNA/RNA helicase, superfamily II 506 e-141 Q64VR8.1 Q64VR8_BACFR ATP-dependent RNA helicase DeaD 499 e-139 >B3KAP4.1 B3KAP4_9BACI DEAD/DEAH box helicase domain protein Length = 491 Score = 623 bits (1606), Expect = e-176, Method: Compositional matrix adjust. Identities = 293/369 (79%), Positives = 335/369 (90%) Query: 16 LSQENYTSPTPIQEESIPAALAGRDVLGCAQTGTGKTAAFSLPIIQLLSQQPSVTGGKRR 75 +++ENY +PTPIQ ++IPA LAGRD+LGCAQTGTGKTAAFS+P+IQLL+QQP G RR Sbjct: 1 MAKENYKAPTPIQAQAIPAVLAGRDLLGCAQTGTGKTAAFSVPMIQLLNQQPPKPGMGRR 60 Query: 76 IRSLILTPTRELALQISENIQAYSKFTNIRSTAIVGGVSQKAQERALSQGADILIATPGR 135 IR+L+L+PTRELALQIS+N++AYS+FT +RSTAIVGGVSQK QERAL QGADILIATPGR Sbjct: 61 IRALVLSPTRELALQISDNVKAYSQFTKLRSTAIVGGVSQKTQERALQQGADILIATPGR 120 Query: 136 LIDLITQKHADLQYVQILVLDEADRMLDMGFIHDVKRIIAKMPSKKQTLFFSATMPTEIS 195 L+DL+ QKH DLQ+V+ILVLDEADRMLDMGFIHDVKRIIAKMPSKKQTLFFSATMP EI+ Sbjct: 121 LLDLMNQKHVDLQHVEILVLDEADRMLDMGFIHDVKRIIAKMPSKKQTLFFSATMPAEIT 180 Query: 196 NLIKTLLVNPVKIEITPVSSTVDRIEQSVYLLENGNKQKQLNRLLQDKSIVSALVFTRTK 255 L++TLL NPVK+EITPVSST +RIEQSVYLLE GNKQ+QLN L++D SIVSALVFTRTK Sbjct: 181 QLVQTLLHNPVKVEITPVSSTAERIEQSVYLLETGNKQQQLNELMKDPSIVSALVFTRTK 240 Query: 256 RGADRVTRDLTRNNITAQAIHGNKSQNDRQNALRNFKSGTTRVLVATDIAARGIDIDELS 315 RGADRV R L + NITAQAIHG+KSQ RQ AL NFKSG TRVLVATDIAARGIDIDELS Sbjct: 241 RGADRVARGLNKVNITAQAIHGDKSQTSRQTALNNFKSGATRVLVATDIAARGIDIDELS 300 Query: 316 HVINFNLPNIPETYVHRIGRTGRAGMSGVAISLCEAEEIPYLKDIQKLIGKTIPEVKDHA 375 HVINFNLPNIPETYVHRIGRTGRAG+SG AIS CE +E+P+L+DI+KLIGK+IPEVKDHA Sbjct: 301 HVINFNLPNIPETYVHRIGRTGRAGLSGTAISFCEVDEVPFLRDIEKLIGKSIPEVKDHA 360 Query: 376 YPMSESAQA 384 +PMS + +A Sbjct: 361 FPMSGALRA 369 >A6EEW7.1 A6EEW7_9SPHI Possible ATP-dependent RNA helicase Length = 435 Score = 510 bits (1313), Expect = e-142, Method: Compositional matrix adjust. Identities = 242/379 (63%), Positives = 300/379 (79%) Query: 1 MTFNDLNLIPAILKALSQENYTSPTPIQEESIPAALAGRDVLGCAQTGTGKTAAFSLPII 60 M F +LNLI ILKAL E YT PTPIQE+SIP L RD+LGCAQTGTGKTAAF++P++ Sbjct: 1 MLFEELNLIEPILKALQTEGYTQPTPIQEQSIPTILTSRDLLGCAQTGTGKTAAFAIPML 60 Query: 61 QLLSQQPSVTGGKRRIRSLILTPTRELALQISENIQAYSKFTNIRSTAIVGGVSQKAQER 120 QLL+++ G R IR+L+LTPTRELA+QI E+ +AY + ++R I GGV QKAQ Sbjct: 61 QLLNKEHQNAKGPRPIRALVLTPTRELAIQIEESFKAYGRNLSLRHLVIFGGVGQKAQTD 120 Query: 121 ALSQGADILIATPGRLIDLITQKHADLQYVQILVLDEADRMLDMGFIHDVKRIIAKMPSK 180 AL +G DILIATPGRL+DL+ Q +L+ ++I VLDEADRMLDMGFIHDVK+ IAK+P+K Sbjct: 121 ALHRGVDILIATPGRLLDLMNQGFINLKDIEIFVLDEADRMLDMGFIHDVKKTIAKLPAK 180 Query: 181 KQTLFFSATMPTEISNLIKTLLVNPVKIEITPVSSTVDRIEQSVYLLENGNKQKQLNRLL 240 +QTLFFSATMP EI L T+LV+P+K+E+TPVSST ++IEQ +Y +E G+K+ L +L Sbjct: 181 RQTLFFSATMPPEIQKLANTILVDPLKVEVTPVSSTAEKIEQMIYFVEKGDKKNLLIHIL 240 Query: 241 QDKSIVSALVFTRTKRGADRVTRDLTRNNITAQAIHGNKSQNDRQNALRNFKSGTTRVLV 300 QDK+I +ALVFTRTK GADRV +DL + + A+AIHGNKSQN RQ AL NFK+ TTR+LV Sbjct: 241 QDKTIQTALVFTRTKHGADRVVKDLIKVGVKAEAIHGNKSQNARQRALTNFKAKTTRILV 300 Query: 301 ATDIAARGIDIDELSHVINFNLPNIPETYVHRIGRTGRAGMSGVAISLCEAEEIPYLKDI 360 ATDIAARGID+DEL+HVIN+ LPNIPETYVHRIGRTGRAG SG++ S C+ EE +L DI Sbjct: 301 ATDIAARGIDVDELTHVINYELPNIPETYVHRIGRTGRAGNSGISFSFCDGEEKEFLDDI 360 Query: 361 QKLIGKTIPEVKDHAYPMS 379 +KLI IP +DH Y MS Sbjct: 361 EKLIALKIPVQEDHPYAMS 379 >C4D4I2.1 C4D4I2_9SPHI DNA/RNA helicase, superfamily II Length = 485 Score = 508 bits (1307), Expect = e-142, Method: Compositional matrix adjust. Identities = 243/377 (64%), Positives = 302/377 (80%), Gaps = 1/377 (0%) Query: 1 MTFNDLNLIPAILKALSQENYTSPTPIQEESIPAALAGRDVLGCAQTGTGKTAAFSLPII 60 M F++L+LI ILKAL++E YT+PTPIQE++IP L+ RD+LGCAQTGTGKTAAF++PI+ Sbjct: 1 MQFSELSLIDPILKALTEEGYTNPTPIQEKAIPILLSRRDLLGCAQTGTGKTAAFAIPIL 60 Query: 61 QLLSQQPS-VTGGKRRIRSLILTPTRELALQISENIQAYSKFTNIRSTAIVGGVSQKAQE 119 QLLS++ S TGG RRI++LILTPTRELA+QI+E+ AY + NIR+T I GGVSQ +Q Sbjct: 61 QLLSEERSKSTGGPRRIKTLILTPTRELAIQIAESFTAYGRHLNIRNTVIFGGVSQHSQV 120 Query: 120 RALSQGADILIATPGRLIDLITQKHADLQYVQILVLDEADRMLDMGFIHDVKRIIAKMPS 179 L G D+LIATPGRL+DL+ Q L+ VQ VLDEADRMLDMGFIHDVK++I K+P+ Sbjct: 121 NTLKAGVDVLIATPGRLLDLMNQGFISLRDVQFFVLDEADRMLDMGFIHDVKKVITKLPT 180 Query: 180 KKQTLFFSATMPTEISNLIKTLLVNPVKIEITPVSSTVDRIEQSVYLLENGNKQKQLNRL 239 +Q+LFFSATMP +++ L T+L NP K+E+TPVSST D IEQ++Y + +K+K L + Sbjct: 181 HRQSLFFSATMPPDVAKLADTILNNPAKVEVTPVSSTADTIEQAMYFVGKEDKRKLLVHI 240 Query: 240 LQDKSIVSALVFTRTKRGADRVTRDLTRNNITAQAIHGNKSQNDRQNALRNFKSGTTRVL 299 L DK+I SALVF RTK GAD+V +DL + I A+AIHGNKSQN RQ AL NFKS TRVL Sbjct: 241 LDDKNIKSALVFARTKHGADKVVKDLLKAGIGAEAIHGNKSQNARQRALSNFKSRETRVL 300 Query: 300 VATDIAARGIDIDELSHVINFNLPNIPETYVHRIGRTGRAGMSGVAISLCEAEEIPYLKD 359 VATDIAARGID+DELSHVIN+ LPNIPETYVHRIGRTGRAG G+A+S C+AEE YL+D Sbjct: 301 VATDIAARGIDVDELSHVINYELPNIPETYVHRIGRTGRAGHDGIALSFCDAEETEYLRD 360 Query: 360 IQKLIGKTIPEVKDHAY 376 I KLIGK +P + +H Y Sbjct: 361 IHKLIGKRVPVIDNHPY 377 >C1YXN6.1 C1YXN6_PEDHE DNA/RNA helicase, superfamily II Length = 437 Score = 506 bits (1302), Expect = e-141, Method: Compositional matrix adjust. Identities = 242/386 (62%), Positives = 304/386 (78%), Gaps = 2/386 (0%) Query: 1 MTFNDLNLIPAILKALSQENYTSPTPIQEESIPAALAGRDVLGCAQTGTGKTAAFSLPII 60 M F +LNLI IL AL E YT PTPIQE++IP+ L RD+LGCAQTGTGKTAAF++P++ Sbjct: 1 MLFKELNLIEPILNALQTEGYTQPTPIQEQAIPSILQNRDLLGCAQTGTGKTAAFAIPML 60 Query: 61 QLLSQQPSVTGGKRRIRSLILTPTRELALQISENIQAYSKFTNIRSTAIVGGVSQKAQER 120 QLLS+ + T + I++L+LTPTRELA+QI E+ +AY K IR I GGV QKAQ Sbjct: 61 QLLSKPHTNTKIHKVIKALVLTPTRELAIQIEESFKAYGKNLPIRHLVIFGGVGQKAQTD 120 Query: 121 ALSQGADILIATPGRLIDLITQKHADLQYVQILVLDEADRMLDMGFIHDVKRIIAKMPSK 180 AL +G DIL+ATPGRL+DL+ Q +L+ ++I VLDEADRMLDMGFIHDVK++IAK+P+K Sbjct: 121 ALHRGVDILVATPGRLLDLMNQGFINLRDIEIFVLDEADRMLDMGFIHDVKKVIAKLPAK 180 Query: 181 KQTLFFSATMPTEISNLIKTLLVNPVKIEITPVSSTVDRIEQSVYLLENGNKQKQLNRLL 240 +QTLFFSATMP EI L T+L NPVK+E+TPVSST ++I Q ++ +E G+K+ L +L Sbjct: 181 RQTLFFSATMPKEIQGLADTILTNPVKVEVTPVSSTAEKIRQEIFYVEKGDKKGLLMHIL 240 Query: 241 QDKSIVSALVFTRTKRGADRVTRDLTRNNITAQAIHGNKSQNDRQNALRNFKSGTTRVLV 300 QDK+I +ALVF RTK GADR+ +DL + + A+AIHGNKSQN RQ AL NFK+ TTR+LV Sbjct: 241 QDKTIETALVFARTKHGADRIVKDLIKVGVKAEAIHGNKSQNARQRALTNFKAKTTRILV 300 Query: 301 ATDIAARGIDIDELSHVINFNLPNIPETYVHRIGRTGRAGMSGVAISLCEAEEIPYLKDI 360 ATDIAARGID+DELSHVIN+ LPNIPETYVHRIGRTGRAG+SG A+S C+AEE +L DI Sbjct: 301 ATDIAARGIDVDELSHVINYELPNIPETYVHRIGRTGRAGLSGTALSFCDAEEKEFLDDI 360 Query: 361 QKLIGKTIPEVKDHAYPMSESAQALL 386 +KLIG +P +DH + M S Q+LL Sbjct: 361 EKLIGLKLPVTEDHPFAM--SWQSLL 384 >Q64VR8.1 Q64VR8_BACFR ATP-dependent RNA helicase DeaD Length = 427 Score = 499 bits (1286), Expect = e-139, Method: Compositional matrix adjust. Identities = 241/384 (62%), Positives = 294/384 (76%), Gaps = 4/384 (1%) Query: 1 MTFNDLNLIPAILKALSQENYTSPTPIQEESIPAALAGRDVLGCAQTGTGKTAAFSLPII 60 MTF +LNLI ILKAL QE YTSPTPIQE+SIP L G+D+LGCAQTGTGKTAAFS+PI+ Sbjct: 1 MTFENLNLIEPILKALRQEGYTSPTPIQEQSIPILLQGKDLLGCAQTGTGKTAAFSIPIL 60 Query: 61 QLLSQQPSVTGGKRRIRSLILTPTRELALQISENIQAYSKFTNIRSTAIVGGVSQKAQER 120 Q L + T ++ I++L+LTPTRELA+QI E+ +AY ++T ++ I GGV QK Q Sbjct: 61 QKLYK----TDHRKGIKALVLTPTRELAIQIGESFEAYGRYTGLKHAVIFGGVGQKPQTD 116 Query: 121 ALSQGADILIATPGRLIDLITQKHADLQYVQILVLDEADRMLDMGFIHDVKRIIAKMPSK 180 AL G IL+ATPGRL+DLI+Q L + VLDEADRMLDMGFIHD+KRI+ +P++ Sbjct: 117 ALRSGIQILVATPGRLLDLISQGFISLSSLDFFVLDEADRMLDMGFIHDIKRILKLLPAR 176 Query: 181 KQTLFFSATMPTEISNLIKTLLVNPVKIEITPVSSTVDRIEQSVYLLENGNKQKQLNRLL 240 +QTLFFSATMP EI L ++L P K+E+TP SSTVD I Q VY +E K+ L LL Sbjct: 177 RQTLFFSATMPPEIETLANSMLTKPEKVEVTPASSTVDIISQQVYFVEKKEKKDLLIHLL 236 Query: 241 QDKSIVSALVFTRTKRGADRVTRDLTRNNITAQAIHGNKSQNDRQNALRNFKSGTTRVLV 300 +D SI S L+FTRTK GAD++ R LT+ I A+AIHGNK+QN RQ AL NFK+ T R L+ Sbjct: 237 KDTSIESVLIFTRTKYGADKLARVLTKAGIGAEAIHGNKTQNARQRALTNFKNHTLRALI 296 Query: 301 ATDIAARGIDIDELSHVINFNLPNIPETYVHRIGRTGRAGMSGVAISLCEAEEIPYLKDI 360 ATDIAARGID+D+LSHVIN+ LPN+PETYVHRIGRTGRAG GVAIS CE+EE+PYLKDI Sbjct: 297 ATDIAARGIDVDQLSHVINYELPNVPETYVHRIGRTGRAGHEGVAISFCESEELPYLKDI 356 Query: 361 QKLIGKTIPEVKDHAYPMSESAQA 384 QKLIGK IP VKDH + +E +A Sbjct: 357 QKLIGKNIPVVKDHPFVTTEGIKA 380 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.131 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,420,467,368 Number of extensions: 89983967 Number of successful extensions: 331327 Number of sequences better than 1.0e-05: 11866 Number of HSP's gapped: 306537 Number of HSP's successfully gapped: 12515 Length of query: 517 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 377 Effective length of database: 1,741,754,103 Effective search space: 656641296831 Effective search space used: 656641296831 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)