BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0276.0002.p (505 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6U2.1 B3K6U2_9BACI Carboxypeptidase Taq EC=3.4.17.19 767 0.0 B1DG10.1 B1DG10_9BACL Carboxypeptidase Taq EC=3.4.17.19 660 0.0 C5D2J4.1 C5D2J4_9BACI Carboxypeptidase Taq EC=3.4.17.19 590 e-167 Q2B409.1 Q2B409_9BACI Thermostable carboxypeptidase 1 590 e-166 A4IN59.1 A4IN59_GEOTN Thermostable carboxypeptidase 1 585 e-165 >B3K6U2.1 B3K6U2_9BACI Carboxypeptidase Taq EC=3.4.17.19 Length = 506 Score = 767 bits (1980), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/499 (71%), Positives = 418/499 (83%) Query: 7 EEWEKFSGLLSKLSGYTEAIGLLHWDLRTGAPRKGVETRSNTIGLLSGEIFRLHTSTEMG 66 ++WE+F L+ K+ Y EAIGLLHWDLRTGAPRKG E RS TIG+LS E F+L S EMG Sbjct: 7 QQWEQFERLVRKIMSYYEAIGLLHWDLRTGAPRKGTEIRSETIGVLSTEAFKLQISEEMG 66 Query: 67 EFTQFFSRPEVMNQLSDVQNKIVKDCRKEYERSQSIPAKSYEEYSVLTAHAQTIWEEAKE 126 E FF++PE QL D + +++++ RK Y++S+SIP + ++EYS+L A ++T WEEAK+ Sbjct: 67 ELLSFFAKPEQAEQLDDRKRRMIEEVRKVYDQSKSIPPERFQEYSILAAQSETKWEEAKK 126 Query: 127 SGDFNSFAPYLSKIVAFTQEFIDHWGVKDSRYDTLLDMYEPDLTVEKLDDIFGRLRSRLV 186 + DF F PYL+KIVAF QEFID+WGVK++RYDTLLDMYEPDLTVEK+D IF RL++RLV Sbjct: 127 NSDFEGFEPYLTKIVAFKQEFIDYWGVKNTRYDTLLDMYEPDLTVEKVDAIFDRLKARLV 186 Query: 187 PLVEAISASPNKPDKEFLAQVFPKEQQEKFGLFILGQMGFDFEAGRLDESVHPFATGLNP 246 PLV AI SP KP +FL Q+FPK QQEKF LF+L QMG+DFEAGRLDESVHPFATGLNP Sbjct: 187 PLVHAIQESPFKPKADFLKQLFPKAQQEKFSLFLLEQMGYDFEAGRLDESVHPFATGLNP 246 Query: 247 GDVRITTNYLPDNVTSAVFSSLHEGGHALYEQNISKELVGTPLAQGASMGIHESQSRLWE 306 GDVRITT YLPD+V SAVFSSLHEGGHALYEQNI KEL GTPL+ G SMGIHESQSRLWE Sbjct: 247 GDVRITTMYLPDDVLSAVFSSLHEGGHALYEQNIDKELAGTPLSGGTSMGIHESQSRLWE 306 Query: 307 NMIGRSRAFWHRYYGDLQQHFPEQLAGVGVEDFYRAINSVANSFIRIEADELTYNLHIII 366 NMIGRSR FW RY+ DLQQHFPEQL V VEDFY A N V NS IRIEADELTYNLHIII Sbjct: 307 NMIGRSRPFWRRYFADLQQHFPEQLKDVDVEDFYLAANKVENSLIRIEADELTYNLHIII 366 Query: 367 RYEIEKLIFNEGLEVKDLPGVWNAKYQECLGITPPSNALGVLQDVHWSGGDFGYFASYSL 426 RYEIEK +FNEGL VKDLP WNAKY+E LGI PP++ +GVLQDVHWSGGDFGYFASYSL Sbjct: 367 RYEIEKKLFNEGLAVKDLPETWNAKYKEYLGIMPPNDGMGVLQDVHWSGGDFGYFASYSL 426 Query: 427 GNMYAAQILNTLRQELPEFEDLIAAGNLLPIKDWLTEKIYRYGQSLTPSQIIEQVTGEPL 486 GNMYAAQ+L+T+R+ELP + LI AGNL PIK+WLT+K+Y+YG+S PS+IIE+VTGE L Sbjct: 427 GNMYAAQMLHTMRKELPNLDQLIEAGNLAPIKEWLTDKVYKYGKSEKPSEIIERVTGEEL 486 Query: 487 NPDYLADYLEAKYTELYQL 505 NPDYLADYLE KY E+Y+L Sbjct: 487 NPDYLADYLEEKYKEIYKL 505 >B1DG10.1 B1DG10_9BACL Carboxypeptidase Taq EC=3.4.17.19 Length = 503 Score = 660 bits (1702), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/500 (62%), Positives = 386/500 (77%), Gaps = 1/500 (0%) Query: 6 KEEWEKFSGLLSKLSGYTEAIGLLHWDLRTGAPRKGVETRSNTIGLLSGEIFRLHTSTEM 65 KE KF + K+ Y EA+G+L+WDLRTGAPRKG++TRS IG LSG++F+L TS E+ Sbjct: 5 KEITAKFMETIRKIKSYEEALGVLYWDLRTGAPRKGMDTRSEVIGALSGDMFKLSTSPEL 64 Query: 66 GEFTQFFSRPEVMNQLSDVQNKIVKDCRKEYERSQSIPAKSYEEYSVLTAHAQTIWEEAK 125 GE+ +P QLSD++ K+V + RK+Y RS IP K Y+EY VL + A++ WEEAK Sbjct: 65 GEYLNELEQPAAQEQLSDIERKLVSETRKDYNRSVKIPPKLYQEYVVLASQAESKWEEAK 124 Query: 126 ESGDFNSFAPYLSKIVAFTQEFIDHWGVKDSRYDTLLDMYEPDLTVEKLDDIFGRLRSRL 185 S D+ SF P L K++ +TQ+FID WG K +RYDTLLD YEP +T +LD +FG LR +L Sbjct: 125 ASNDYASFQPLLEKVIDYTQQFIDLWGPKATRYDTLLDQYEPGMTTAELDRVFGGLREQL 184 Query: 186 VPLVEAISASPNKPDKEFLAQVFPKEQQEKFGLFILGQMGFDFEAGRLDESVHPFATGLN 245 VPL AI+ASP++PD FL Q + K+ Q+ F L+IL QMG++FEAGRLDESVHPFATGLN Sbjct: 185 VPLAAAIAASPHQPDTSFLGQKYDKQAQKAFSLYILNQMGYNFEAGRLDESVHPFATGLN 244 Query: 246 PGDVRITTNYLPDNVTSAVFSSLHEGGHALYEQNISKELVGTPLAQGASMGIHESQSRLW 305 PGDVRITT YL D+VTSA+F ++HEGGHALYEQNI EL+GT L G SMGIHESQSR W Sbjct: 245 PGDVRITTRYLEDDVTSALFGTIHEGGHALYEQNIMPELIGTTLCTGTSMGIHESQSRFW 304 Query: 306 ENMIGRSRAFWHRYYGDLQQHFPEQLAGVGVEDFYRAINSVANSFIRIEADELTYNLHII 365 EN+IGRS+ FW +Y+G LQ+ FP QL V VE FYR IN V S IRIEADELTYNLHII Sbjct: 305 ENVIGRSKPFWEQYFGKLQEKFPGQL-DVSVEQFYRGINVVQPSLIRIEADELTYNLHII 363 Query: 366 IRYEIEKLIFNEGLEVKDLPGVWNAKYQECLGITPPSNALGVLQDVHWSGGDFGYFASYS 425 IRYEIEK+IFNEG + DLP +WN KY+E LGI PPSNA GVLQDVHWSGG FGYF SYS Sbjct: 364 IRYEIEKMIFNEGAKAADLPAIWNDKYKEYLGIVPPSNAEGVLQDVHWSGGAFGYFPSYS 423 Query: 426 LGNMYAAQILNTLRQELPEFEDLIAAGNLLPIKDWLTEKIYRYGQSLTPSQIIEQVTGEP 485 LGNMYAAQI +T+ +EL F +L+ G LLPIK+WLTE+IY++G+ +PS+II+ VTG+P Sbjct: 424 LGNMYAAQIADTMERELDGFWNLVGRGELLPIKEWLTERIYKFGKLKSPSEIIQSVTGKP 483 Query: 486 LNPDYLADYLEAKYTELYQL 505 L+P +L YLE KY ++Y+L Sbjct: 484 LDPQHLVSYLERKYKDIYKL 503 >C5D2J4.1 C5D2J4_9BACI Carboxypeptidase Taq EC=3.4.17.19 Length = 500 Score = 590 bits (1522), Expect = e-167, Method: Compositional matrix adjust. Identities = 277/503 (55%), Positives = 372/503 (73%), Gaps = 4/503 (0%) Query: 4 LVKEEWEKFSGLLSKLSGYTEAIGLLHWDLRTGAPRKGVETRSNTIGLLSGEIFRLHTST 63 ++K+ E+F + K+ Y EAI L++WDLRTGAP+KGVE RS IG+LS E+F++ TS Sbjct: 1 MIKKIEEQFLQYVKKMMSYNEAIQLMYWDLRTGAPKKGVEQRSEVIGILSQEVFKMSTSE 60 Query: 64 EMGEF-TQFFSRPEVMNQLSDVQNKIVKDCRKEYERSQSIPAKSYEEYSVLTAHAQTIWE 122 EM F + + E QLS+V +++C+KEYER++ IPA Y+EY VL + A+++WE Sbjct: 61 EMAAFIAKLLPKHE---QLSEVTRYTLEECKKEYERNKKIPADEYKEYVVLQSKAESVWE 117 Query: 123 EAKESGDFNSFAPYLSKIVAFTQEFIDHWGVKDSRYDTLLDMYEPDLTVEKLDDIFGRLR 182 EAK + DF F PYL K+V F + FI++WG K + Y+TLLD+YEP +TV+ LD++F +LR Sbjct: 118 EAKATADFALFRPYLEKLVEFNKRFIEYWGYKGNPYNTLLDLYEPGMTVDVLDEVFRKLR 177 Query: 183 SRLVPLVEAISASPNKPDKEFLAQVFPKEQQEKFGLFILGQMGFDFEAGRLDESVHPFAT 242 +VPLV+ ++ASPN+P+ FL FPKE+Q F L +L ++G+DF GRLDE+VHPFA Sbjct: 178 EHIVPLVQEVAASPNQPETSFLFHRFPKEKQRAFSLELLRELGYDFAKGRLDETVHPFAI 237 Query: 243 GLNPGDVRITTNYLPDNVTSAVFSSLHEGGHALYEQNISKELVGTPLAQGASMGIHESQS 302 GLNP DVRITT Y + +A+F ++HE GHALYEQ+IS+ L+GTPL G SMGIHESQS Sbjct: 238 GLNPNDVRITTRYDERDFRTAIFGTIHECGHALYEQHISETLIGTPLCTGTSMGIHESQS 297 Query: 303 RLWENMIGRSRAFWHRYYGDLQQHFPEQLAGVGVEDFYRAINSVANSFIRIEADELTYNL 362 +EN I R ++W R Y LQQ+ PEQ +GV ++ FYRAIN V S IRIEADELTY L Sbjct: 298 LFYENFIARHYSYWKRNYPRLQQYAPEQFSGVSLDAFYRAINEVKPSLIRIEADELTYPL 357 Query: 363 HIIIRYEIEKLIFNEGLEVKDLPGVWNAKYQECLGITPPSNALGVLQDVHWSGGDFGYFA 422 HII+RYEIEK +FN +EVKDLP +WN KY+E LGI P ++A+GVLQDVHWSGG FGYF Sbjct: 358 HIIVRYEIEKGLFNGTIEVKDLPEIWNDKYKEYLGIRPDNDAVGVLQDVHWSGGSFGYFP 417 Query: 423 SYSLGNMYAAQILNTLRQELPEFEDLIAAGNLLPIKDWLTEKIYRYGQSLTPSQIIEQVT 482 SY+LG MYAAQ + + ++LP F++L+ G L PI++WLTE+I+R+G++ P +I+++ T Sbjct: 418 SYALGYMYAAQFKHAMLKDLPHFDELLEKGELTPIREWLTERIHRFGKTKKPLEILQEAT 477 Query: 483 GEPLNPDYLADYLEAKYTELYQL 505 GE LN DYL YLE KY +YQL Sbjct: 478 GEALNADYLIRYLEEKYKAIYQL 500 >Q2B409.1 Q2B409_9BACI Thermostable carboxypeptidase 1 Length = 510 Score = 590 bits (1520), Expect = e-166, Method: Compositional matrix adjust. Identities = 270/505 (53%), Positives = 376/505 (74%) Query: 1 MEQLVKEEWEKFSGLLSKLSGYTEAIGLLHWDLRTGAPRKGVETRSNTIGLLSGEIFRLH 60 M + +K+ + F + K+ Y EA+ L++WDLRTGAP++GV+ R+ IG+LS E+F++ Sbjct: 1 MNEALKKTEKDFLDFVKKMGAYNEALSLIYWDLRTGAPKQGVDQRTEVIGMLSSEVFKMS 60 Query: 61 TSTEMGEFTQFFSRPEVMNQLSDVQNKIVKDCRKEYERSQSIPAKSYEEYSVLTAHAQTI 120 TS EM + S E L + K++++ +KEY+R++ IPA+ Y+EY +L + A+++ Sbjct: 61 TSEEMAAYIAKLSGEEAAASLDAITVKVLEESKKEYDRNKKIPAEEYKEYVILQSKAESV 120 Query: 121 WEEAKESGDFNSFAPYLSKIVAFTQEFIDHWGVKDSRYDTLLDMYEPDLTVEKLDDIFGR 180 WEEAKE DF F PYL K+V + FI +WG + ++Y+TLLD+YEP +TV+ LD++FG Sbjct: 121 WEEAKEKSDFTLFQPYLEKLVEANKRFIQYWGYEGNKYNTLLDLYEPGVTVDVLDEVFGE 180 Query: 181 LRSRLVPLVEAISASPNKPDKEFLAQVFPKEQQEKFGLFILGQMGFDFEAGRLDESVHPF 240 LRS++VPLV++IS S +KPD FL + FPKE+Q F L IL QMG++F+AGRLDE+VHPF Sbjct: 181 LRSKIVPLVKSISESDSKPDTSFLYENFPKEKQRDFSLEILKQMGYNFDAGRLDETVHPF 240 Query: 241 ATGLNPGDVRITTNYLPDNVTSAVFSSLHEGGHALYEQNISKELVGTPLAQGASMGIHES 300 ATGLNPGDVR+TT Y + +AVF ++HEGGHALYEQNISK+L+GTPL G SMGIHES Sbjct: 241 ATGLNPGDVRVTTKYDEKDFRTAVFGTIHEGGHALYEQNISKDLIGTPLCTGTSMGIHES 300 Query: 301 QSRLWENMIGRSRAFWHRYYGDLQQHFPEQLAGVGVEDFYRAINSVANSFIRIEADELTY 360 QS +EN +GRS +FW + YG L+Q+ Q GV +EDFYRAIN S IRIEADELTY Sbjct: 301 QSLFYENFVGRSESFWKKNYGLLKQYADGQFDGVELEDFYRAINESKPSLIRIEADELTY 360 Query: 361 NLHIIIRYEIEKLIFNEGLEVKDLPGVWNAKYQECLGITPPSNALGVLQDVHWSGGDFGY 420 LH+I+RYEIEK +FN+ +EVKDLP +WN+KY+E LG+ P ++A GVLQDVHW+GG FGY Sbjct: 361 PLHVIVRYEIEKGLFNDEIEVKDLPEIWNSKYEEYLGVRPSNDAEGVLQDVHWAGGSFGY 420 Query: 421 FASYSLGNMYAAQILNTLRQELPEFEDLIAAGNLLPIKDWLTEKIYRYGQSLTPSQIIEQ 480 F SY+LG MYAAQ+ L ++LP+++ L+ G+L P+K+W T+ +++YG+ P +I+E Sbjct: 421 FPSYALGYMYAAQLKQALLKDLPDYDQLLEKGDLAPVKEWFTKNVHQYGKLKKPLEILED 480 Query: 481 VTGEPLNPDYLADYLEAKYTELYQL 505 VTGE LN L++YL KY ++Y+L Sbjct: 481 VTGEGLNASCLSEYLYEKYGKVYRL 505 >A4IN59.1 A4IN59_GEOTN Thermostable carboxypeptidase 1 Length = 501 Score = 585 bits (1508), Expect = e-165, Method: Compositional matrix adjust. Identities = 284/502 (56%), Positives = 357/502 (71%), Gaps = 1/502 (0%) Query: 4 LVKEEWEKFSGLLSKLSGYTEAIGLLHWDLRTGAPRKGVETRSNTIGLLSGEIFRLHTST 63 +VK E+F + K+ GY EAI L++WDLRTGAP+KGVE RS IG+LS E F++ TS Sbjct: 1 MVKRMEEQFLQYVKKMMGYHEAINLMYWDLRTGAPKKGVEQRSEVIGMLSEEAFKMSTSE 60 Query: 64 EMGEFTQFFSRPEVMNQLSDVQNKIVKDCRKEYERSQSIPAKSYEEYSVLTAHAQTIWEE 123 EM F S EV QL +V + +C+KEYER++ IP Y+EY VL + A+++WEE Sbjct: 61 EMAAFIAKLSPKEVYEQLDEVTKHTLDECKKEYERNKKIPVDEYKEYVVLCSKAESVWEE 120 Query: 124 AKESGDFNSFAPYLSKIVAFTQEFIDHWGVKDSRYDTLLDMYEPDLTVEKLDDIFGRLRS 183 AK + DF F PYL +I+ F + FI +WG + Y+TLLD YEP +TVE LD +FG+LR Sbjct: 121 AKAAADFARFRPYLEQIIEFQRRFISYWGYEGHPYNTLLDQYEPGMTVELLDGLFGQLRE 180 Query: 184 RLVPLVEAISASPNKPDKEFLAQVFPKEQQEKFGLFILGQMGFDFEAGRLDESVHPFATG 243 R+VPLV AISA +KPD FL FPKE+Q F L +L ++G+DF GRLDE+VHPFA G Sbjct: 181 RIVPLVHAISAVSDKPDTSFLFAPFPKEKQRAFLLELLRELGYDFGKGRLDETVHPFAIG 240 Query: 244 LNPGDVRITTNYLPDNVTSAVFSSLHEGGHALYEQNISKELVGTPLAQGASMGIHESQSR 303 LNP DVRITT Y + +AVF ++HE GHALYEQ+IS+ LVGTPLA GASMGIHESQS Sbjct: 241 LNPNDVRITTRYDERDFRTAVFGTIHECGHALYEQHISEALVGTPLASGASMGIHESQSL 300 Query: 304 LWENMIGRSRAFWHRYYGDLQQHFPEQLAGVGVEDFYRAINSVANSFIRIEADELTYNLH 363 ENMIGR +FW R+Y LQQ+ PEQ A V ++ FYRAIN S IRIEADELTY+LH Sbjct: 301 FLENMIGRHPSFWKRHYPRLQQYAPEQFADVSLDAFYRAINEAKPSLIRIEADELTYSLH 360 Query: 364 IIIRYEIEKLIFNEGLEVKDLPGVWNAKYQECLGITPPSNALGVLQDVHWSGGDFGYFAS 423 I++RYEIEK +F LE DLP VWN KY++ LGI P ++A+GVLQDVHWSGG FGYF S Sbjct: 361 IMVRYEIEKQLFAGELEAADLPDVWNEKYEQYLGIRPHNDAVGVLQDVHWSGGSFGYFPS 420 Query: 424 YSLGNMYAAQILNTLRQELPEFEDLIAAGNLLPIKDWLTEKIYRYGQSLTPSQIIEQVTG 483 Y+LG MYAAQ L +EL + L+ GN+ PI++WLT I+R+G++ P ++ TG Sbjct: 421 YALGYMYAAQFKQALEKEL-DLAQLLEEGNITPIREWLTANIHRFGKTKKPLDLVRDATG 479 Query: 484 EPLNPDYLADYLEAKYTELYQL 505 EPL DYL YLEAKY LY+L Sbjct: 480 EPLKADYLIQYLEAKYKPLYRL 501 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,182,037,400 Number of extensions: 138832676 Number of successful extensions: 325537 Number of sequences better than 1.0e-05: 539 Number of HSP's gapped: 323651 Number of HSP's successfully gapped: 540 Length of query: 505 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 365 Effective length of database: 1,741,754,103 Effective search space: 635740247595 Effective search space used: 635740247595 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)