BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0276.0001.p (236 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KGJ3.1 B3KGJ3_9BACI Channel protein, hemolysin III family 248 4e-64 Q5WEZ3.1 Q5WEZ3_BACSK Hemolysin 197 5e-49 Q9K8Y7.1 Q9K8Y7_BACHD Hemolysin III 195 2e-48 Q8EP52.1 Q8EP52_OCEIH Hemolysin III 192 2e-47 B1DE24.1 B1DE24_9BACL Channel protein, hemolysin III family 192 2e-47 >B3KGJ3.1 B3KGJ3_9BACI Channel protein, hemolysin III family Length = 215 Score = 248 bits (632), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 122/214 (57%), Positives = 145/214 (67%) Query: 22 MANTHTYSRKEEVANAVTHGIGXXXXXXXXXXXXXXXXQNGTAWHVVSFSIYGATMLLLY 81 MANT+TY R+EE+ANA+THGIG GTAWHVVSF+IYG+TMLLLY Sbjct: 1 MANTYTYPRREEIANAITHGIGAALSVAALVLLIVFSSLKGTAWHVVSFTIYGSTMLLLY 60 Query: 82 INSTMVHSLKEGKAKDFFEFLDHSSIYLFIAGTYTPFMLVAIRGTLGWTXXXXXXXXXXX 141 ++ST+VH L++GKAKDFFEF+DHSSIYLFIAGTYTPF+LVAIRG LGW+ Sbjct: 61 LSSTLVHGLRDGKAKDFFEFMDHSSIYLFIAGTYTPFLLVAIRGPLGWSLFGIVWGIALF 120 Query: 142 XXXXXXXXXXXXXXMSTIFYIAMGWMIVIAWNPLSAVXXXXXXXXXXXXXXXXXXXXVFY 201 +STIFYIAMGW+IVIAWNPL+AV +FY Sbjct: 121 GVVFKAFFVKKFLFLSTIFYIAMGWLIVIAWNPLTAVVAPQGMNLLVLGGVLYTLGTIFY 180 Query: 202 VWRGFPYHHAIWHLFVLGGTVAHFFVVLLYVLPI 235 VWRGFPYHHAIWHLFVL G++ HFF +L+Y+LPI Sbjct: 181 VWRGFPYHHAIWHLFVLAGSILHFFAILIYLLPI 214 >Q5WEZ3.1 Q5WEZ3_BACSK Hemolysin Length = 216 Score = 197 bits (502), Expect = 5e-49, Method: Compositional matrix adjust. Identities = 99/214 (46%), Positives = 128/214 (59%) Query: 22 MANTHTYSRKEEVANAVTHGIGXXXXXXXXXXXXXXXXQNGTAWHVVSFSIYGATMLLLY 81 MANTH +SR+EE+ NA+THG G +GTAWH+VSF+IYG +M+LLY Sbjct: 1 MANTHVFSRREELVNALTHGFGALLSILALVLLIVYSSMHGTAWHIVSFTIYGTSMVLLY 60 Query: 82 INSTMVHSLKEGKAKDFFEFLDHSSIYLFIAGTYTPFMLVAIRGTLGWTXXXXXXXXXXX 141 ++ST+VHS K GKAKD FE DH++IYLFIAGTYTP + + + G LGWT Sbjct: 61 LSSTLVHSFKPGKAKDVFEIFDHAAIYLFIAGTYTPILFILVGGALGWTLFGIVWGIAVV 120 Query: 142 XXXXXXXXXXXXXXMSTIFYIAMGWMIVIAWNPLSAVXXXXXXXXXXXXXXXXXXXXVFY 201 +STI Y+AMGW+ V A P+ + +FY Sbjct: 121 GIIFKVFYVKKFLVLSTIMYVAMGWIAVFAMGPIMSTLPSGGIWLLVLGGLFYTAGTIFY 180 Query: 202 VWRGFPYHHAIWHLFVLGGTVAHFFVVLLYVLPI 235 VWRGF YHHAIWHLFVL G+V+HFF+V L++LP+ Sbjct: 181 VWRGFLYHHAIWHLFVLAGSVSHFFLVFLFILPV 214 >Q9K8Y7.1 Q9K8Y7_BACHD Hemolysin III Length = 215 Score = 195 bits (496), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 98/214 (45%), Positives = 125/214 (58%) Query: 22 MANTHTYSRKEEVANAVTHGIGXXXXXXXXXXXXXXXXQNGTAWHVVSFSIYGATMLLLY 81 M TH +SRKEE+ANA+THGIG G+A HVVSF++YG TML+LY Sbjct: 1 MGTTHIFSRKEELANAITHGIGVVLSLAALVVLIVFASLYGSAMHVVSFTVYGTTMLMLY 60 Query: 82 INSTMVHSLKEGKAKDFFEFLDHSSIYLFIAGTYTPFMLVAIRGTLGWTXXXXXXXXXXX 141 +STM+HSL EGK KD FE +DH++IYLFIAGTYTP + + ++G LGWT Sbjct: 61 FSSTMLHSLPEGKVKDIFEVIDHAAIYLFIAGTYTPLLFIVVQGALGWTLFGVVWGLAVV 120 Query: 142 XXXXXXXXXXXXXXMSTIFYIAMGWMIVIAWNPLSAVXXXXXXXXXXXXXXXXXXXXVFY 201 +ST Y+AMGW+ VIA+ P+ +FY Sbjct: 121 GIVFKAFFVKRFLFLSTACYVAMGWLAVIAFRPIMETLPSGGIACLVGGGIAYTVGTIFY 180 Query: 202 VWRGFPYHHAIWHLFVLGGTVAHFFVVLLYVLPI 235 VWRGF YHHAIWH+FVL G++ HF ++L YVLPI Sbjct: 181 VWRGFSYHHAIWHVFVLIGSILHFVLILFYVLPI 214 >Q8EP52.1 Q8EP52_OCEIH Hemolysin III Length = 213 Score = 192 bits (488), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 96/213 (45%), Positives = 126/213 (59%) Query: 22 MANTHTYSRKEEVANAVTHGIGXXXXXXXXXXXXXXXXQNGTAWHVVSFSIYGATMLLLY 81 MANTH +++ EE+ANA+THGI NGTA HVV+F+I+GATM++LY Sbjct: 1 MANTHIFTKGEEIANAITHGIAALLSVAGLVVLIVSSVFNGTALHVVTFTIFGATMVILY 60 Query: 82 INSTMVHSLKEGKAKDFFEFLDHSSIYLFIAGTYTPFMLVAIRGTLGWTXXXXXXXXXXX 141 +ST VH+L +GKAKD FE LDHSSIYLFIAGTYTPF L+ I+G +GW+ Sbjct: 61 TSSTFVHALPKGKAKDIFEILDHSSIYLFIAGTYTPFTLLVIQGAMGWSIFGVVWGIALF 120 Query: 142 XXXXXXXXXXXXXXMSTIFYIAMGWMIVIAWNPLSAVXXXXXXXXXXXXXXXXXXXXVFY 201 ST+ YI+MGW+IVI W ++ +FY Sbjct: 121 GIVFKIYFVKRFLFTSTLLYISMGWLIVIGWTQITNNLESSGVYLLVAGGLCYTLGTIFY 180 Query: 202 VWRGFPYHHAIWHLFVLGGTVAHFFVVLLYVLP 234 +WRGF +HH IWHLFV+ GT+ H+F VL ++LP Sbjct: 181 MWRGFKFHHMIWHLFVIAGTIFHYFCVLFFLLP 213 >B1DE24.1 B1DE24_9BACL Channel protein, hemolysin III family Length = 211 Score = 192 bits (488), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 99/211 (46%), Positives = 120/211 (56%) Query: 22 MANTHTYSRKEEVANAVTHGIGXXXXXXXXXXXXXXXXQNGTAWHVVSFSIYGATMLLLY 81 MANTH YS+KEE ANA+THGIG G AWH+VSF ++G M +LY Sbjct: 1 MANTHVYSKKEEAANAITHGIGAGLSIAALVLLIVRASYIGEAWHIVSFILFGFFMFILY 60 Query: 82 INSTMVHSLKEGKAKDFFEFLDHSSIYLFIAGTYTPFMLVAIRGTLGWTXXXXXXXXXXX 141 ++ST++HS EGK KD FE DH SIYLFIAGTYTP++LV IRG LGW+ Sbjct: 61 MSSTLLHSFPEGKVKDLFEIFDHGSIYLFIAGTYTPYLLVTIRGPLGWSLFGVIWGLAIS 120 Query: 142 XXXXXXXXXXXXXXMSTIFYIAMGWMIVIAWNPLSAVXXXXXXXXXXXXXXXXXXXXVFY 201 +ST+ Y+ MGWMIV AW L VFY Sbjct: 121 GVVFKCFFVKRFLFLSTLGYVIMGWMIVFAWGALVDHLAYGGLVFLVVGGVCYTVGSVFY 180 Query: 202 VWRGFPYHHAIWHLFVLGGTVAHFFVVLLYV 232 VWRGF YHHA+WHLFV+ G++ HFF +LLYV Sbjct: 181 VWRGFLYHHAVWHLFVIAGSICHFFSILLYV 211 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.141 0.460 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,061,210,543 Number of extensions: 29936750 Number of successful extensions: 74407 Number of sequences better than 1.0e-05: 950 Number of HSP's gapped: 72087 Number of HSP's successfully gapped: 963 Length of query: 236 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 104 Effective length of database: 1,817,122,223 Effective search space: 188980711192 Effective search space used: 188980711192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 129 (54.3 bits)