BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0274.0007.p (577 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A5ZID8.1 A5ZID8_9BACE Putative uncharacterized protein 280 3e-73 B0URH6.1 B0URH6_HAES2 Glycoside hydrolase family 3 domain protein 244 3e-62 Q0I582.1 Q0I582_HAES1 Possible beta-hexosamidase A, glycoside hy... 243 3e-62 B0A6F5.1 B0A6F5_9CLOT Putative uncharacterized protein 242 6e-62 Q1J2S2.1 Q1J2S2_DEIGD Glycoside hydrolase, family 3-like protein 239 6e-61 >A5ZID8.1 A5ZID8_9BACE Putative uncharacterized protein Length = 586 Score = 280 bits (716), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 187/562 (33%), Positives = 277/562 (49%), Gaps = 39/562 (6%) Query: 15 VERTLTSMSLREKIGQTMQDHAGRLPFKGNDEEALAAYLKTYPVGSFFIGGEVIQKAAGK 74 V+ T ++LREKIGQTM R + +L Y K YPV +F+G ++ G Sbjct: 39 VDSTWKLLTLREKIGQTMLMLPDRKKELDLGDGSLDVYFKRYPVTGYFMGWKLF---TGV 95 Query: 75 AEE-----YRDWAEQLQRISKYPLLFSGDLEFGAGSAVKSLTAFPPXXXXXXXXXXXXXX 129 EE R E+ Q+ S+ PLLF D E G G ++ +T FP Sbjct: 96 PEEERVDFVRSSVEEYQKASELPLLFQQDYESGVG--LQGMTPFPKEMALGAANSPELAY 153 Query: 130 XXXKFTALEGRAAGFTWALAPGTDLLLNWMNPVITTRCXXXXXXXXXXXXXXVMRGMQDH 189 K ALE R+ G W L P DL +N +NP++ TR ++G+QD+ Sbjct: 154 KYGKSVALECRSLGINWVLHPVADLNMNPLNPIVNTRSVSDNPEKAVCLLSEQIKGLQDN 213 Query: 190 GLAACAKHFPGDGVDYRDQHIVTTINGLSEEEWFAAYGQVSQHMIDQGVLSYMTGHIALP 249 +AA KHFPGDGVDYRDQH++TT+N LSEE W +G+V +I +GV M GHI LP Sbjct: 214 SVAATIKHFPGDGVDYRDQHLMTTVNSLSEEMWKQYHGKVFAELIKKGVACIMPGHITLP 273 Query: 250 WLEGGTQEEKPVPATVSERITTGLLRERMGFAGVVLSDALDMGGFLAWGSYE-KRMVDCF 308 + + PAT+S + TGLL++ M F GVV+SDA+ M GF W + + V F Sbjct: 274 FYQKEKINGYLPPATLSHELLTGLLKKEMHFNGVVVSDAMTMAGFRGWYKNDLEGQVASF 333 Query: 309 NSGTDVLLWPGVEYFGVMEQAVSEGTVLMERLDASVRRVLELKALLGVHRVDQKGRDPQA 368 +G D+LLWP EY +E + G + MERLD +VRRV +K G+ +++ P Sbjct: 334 LAGVDILLWPSYEYMDTVEVRIQRGEIPMERLDDAVRRVWAMKERFGLLNKNRELIRP-- 391 Query: 369 VPLLNDRLPPKLDLEARQLSRTIAERCITLVRNRGGILPLDPQTTRRVLVVLLNKVT--- 425 + + E R+ ++ I ER ITLVR+ LPL + +++L+V + V Sbjct: 392 -------VSAEEKAEIREAAKEITERSITLVRDEDHKLPLSLEKDKKLLLVAVTPVAHKG 444 Query: 426 EGRKYERMNVFVELLRARGLQVDILDEFEPLGTLRKWELSGIR-WDAAFAPYFLPLHGMM 484 R ++R+ + G +VD L + W+ + +D FL Sbjct: 445 NDRDFKRLQNLRDEFVKNGFEVDFRRNI--LYEDQGWQDNATEVYDKVI---FLVAR--- 496 Query: 485 NTARPVG------EAAKAIWAMQHAETIRPIGISFATPYLLQD-MPFLDALINAYSLHEE 537 NT P G + A+++WA + + I IS +PY+ + + IN YS Sbjct: 497 NTHTPFGPLQLWDDEAQSVWAANSMDKSKVIVISLGSPYIGNEYFERVKTYINTYSNDAS 556 Query: 538 TVELTVKALFGEVPFQGQSPVK 559 T ++ L G++PF+G+SPV Sbjct: 557 THSALLRILLGQLPFKGKSPVN 578 >B0URH6.1 B0URH6_HAES2 Glycoside hydrolase family 3 domain protein Length = 586 Score = 244 bits (622), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 180/584 (30%), Positives = 268/584 (45%), Gaps = 48/584 (8%) Query: 7 LTREEQDWVERTLTSMSLREKIGQTMQDHAGRLPFKGNDEEALAAYLKTYPVGSFFIGGE 66 L E+ WV+ T+ +M+L EKIGQ + EE L L Y +G+ Sbjct: 12 LKDEDIQWVKNTIANMTLEEKIGQLFVNMGS-----SRSEEYLTDVLNRYHIGA------ 60 Query: 67 VIQKAAGKAEEYRDWAEQLQRISKYPLLFSGDLEFGAGSAVKSLTAFPPXXXXXXXXXXX 126 ++ G AEE D LQ SK PLL + + E G A T Sbjct: 61 -VRYNPGPAEEVYDQNYILQTKSKIPLLIAANTEAGGNGACSDGTEIGLQVKIGATDDAK 119 Query: 127 XXXXXXKFTALEGRAAGFTWALAPGTDLLLNWMNPVITTRCXXXXXXXXXXXXXXVMRGM 186 + +E A G W+ AP D+ NW NP+I+ R M+G+ Sbjct: 120 YAYEMGRVAGIEAAAIGCNWSFAPIVDISYNWRNPIISNRVFGSNPEKVLEMSLAYMKGI 179 Query: 187 QDHGLAACAKHFPGDGVDYRDQHIVTTINGLSEEEWFAAYGQVSQHMIDQGVLSYMTGHI 246 Q+ G+A AKHFPGDGVD RDQH+ ++N S EEW +G+V Q +ID G+ S M GHI Sbjct: 180 QESGIAPAAKHFPGDGVDERDQHLSFSVNSFSCEEWDKTFGKVYQGLIDAGLPSLMAGHI 239 Query: 247 ALPWLEG----GTQEEKPVPATVSERITTGLLRERMGFAGVVLSDALDMGGFLAWGSYEK 302 LP E E +PAT+S+ I T LLR ++GF GVV++DA M + + Sbjct: 240 HLPAYEQHFNPNLAYEDCLPATLSKPILTDLLRGKLGFNGVVVTDASHMVAMTSAMKRSE 299 Query: 303 RMVDCFNSGTDVLLWPG--VEYFGVMEQAVSEGTVLMERLDASVRRVLELKALLGVH-RV 359 + +G D+ L+ E FG M + G + ERL ++ R+L LKA LG+H R Sbjct: 300 MLPTAIAAGCDLFLFFNDPDEDFGYMMEGYKNGIITEERLHDALTRILGLKAKLGLHNRP 359 Query: 360 DQKGRDPQAVPLLNDRLPPKLDLEARQLSRTIAERCITLVRNRGGILPLDPQTTRRVLVV 419 + +P+ L LP E + + R +A++ ITLV+++ I P++ + R+L+V Sbjct: 360 RETLLEPKEQALAKIGLP-----EHKAIFRDVADKAITLVKHKQDIFPINVERFPRILLV 414 Query: 420 LLN-------KVTEGRKYERMNVFVELLRARGLQVDI----------LDEFEPLGTLRKW 462 + K+ G + + E L +G V I + + E + T+R Sbjct: 415 NVKGTDGGFGKMVAGSQRSATEILKEKLEEKGFTVSIYTSPMDNILKMSDEEQVKTIRN- 473 Query: 463 ELSGIRWDAAFAPYFLPLHGMMN-----TARPVGEAAKAIWAMQ-HAETIRPIGISFATP 516 E S R ++ + + N R V +A K + + + I +S P Sbjct: 474 EYSQKRPITTLTDHYDLIINVANVQMSTVQRIVWQATKGTPDIPFYVHEVPTIFVSVQCP 533 Query: 517 YLLQDMPFLDALINAYSLHEETVELTVKALFGEVPFQGQSPVKA 560 + L D+P + INAY E T+EL V+ L G F+G SPV A Sbjct: 534 FHLVDVPQVKTYINAYDGKEPTIELLVEKLMGNSEFKGISPVDA 577 >Q0I582.1 Q0I582_HAES1 Possible beta-hexosamidase A, glycoside hydrolase family 3 Length = 586 Score = 243 bits (621), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 180/584 (30%), Positives = 268/584 (45%), Gaps = 48/584 (8%) Query: 7 LTREEQDWVERTLTSMSLREKIGQTMQDHAGRLPFKGNDEEALAAYLKTYPVGSFFIGGE 66 L E+ WV+ T+ +M+L EKIGQ + EE L L Y +G+ Sbjct: 12 LKDEDIQWVKNTIANMTLEEKIGQLFVNMGS-----SRSEEYLTDVLNRYHIGA------ 60 Query: 67 VIQKAAGKAEEYRDWAEQLQRISKYPLLFSGDLEFGAGSAVKSLTAFPPXXXXXXXXXXX 126 ++ G AEE D LQ SK PLL + + E G A T Sbjct: 61 -VRYNPGPAEEVYDQNYILQTKSKIPLLIAANTEAGGNGACSDGTEIGLQVKIGATDDAK 119 Query: 127 XXXXXXKFTALEGRAAGFTWALAPGTDLLLNWMNPVITTRCXXXXXXXXXXXXXXVMRGM 186 + +E A G W+ AP D+ NW NP+I+ R M+G+ Sbjct: 120 YAYEMGRVAGIEAAAIGCNWSFAPIVDISYNWRNPIISNRVFGSNPEKVLEMSLAYMKGI 179 Query: 187 QDHGLAACAKHFPGDGVDYRDQHIVTTINGLSEEEWFAAYGQVSQHMIDQGVLSYMTGHI 246 Q+ G+A AKHFPGDGVD RDQH+ ++N S EEW +G+V Q +ID G+ S M GHI Sbjct: 180 QESGIAPAAKHFPGDGVDERDQHLSFSVNSFSCEEWDNTFGKVYQGLIDAGLPSLMAGHI 239 Query: 247 ALPWLEG----GTQEEKPVPATVSERITTGLLRERMGFAGVVLSDALDMGGFLAWGSYEK 302 LP E E +PAT+S+ I T LLR ++GF GVV++DA M + + Sbjct: 240 HLPAYEQHFNPNLAYEDCLPATLSKPILTDLLRGKLGFNGVVVTDASHMVAMTSAMKRSE 299 Query: 303 RMVDCFNSGTDVLLWPG--VEYFGVMEQAVSEGTVLMERLDASVRRVLELKALLGVH-RV 359 + +G D+ L+ E FG M + G + ERL ++ R+L LKA LG+H R Sbjct: 300 MLPTAIAAGCDLFLFFNDPDEDFGYMMEGYKNGIITEERLHDALTRILGLKAKLGLHNRP 359 Query: 360 DQKGRDPQAVPLLNDRLPPKLDLEARQLSRTIAERCITLVRNRGGILPLDPQTTRRVLVV 419 + +P+ L LP E + + R +A++ ITLV+++ I P++ + R+L+V Sbjct: 360 RETLLEPKEQALAKIGLP-----EHKAIFRDVADKAITLVKHKQDIFPINVERFPRILLV 414 Query: 420 LLN-------KVTEGRKYERMNVFVELLRARGLQVDI----------LDEFEPLGTLRKW 462 + K+ G + + E L +G V I + + E + T+R Sbjct: 415 NVKGTDGGFGKMVAGSQRSATEILKEKLEEKGFTVSIYTSPMDNILKMSDEEQVKTIRN- 473 Query: 463 ELSGIRWDAAFAPYFLPLHGMMN-----TARPVGEAAKAIWAMQ-HAETIRPIGISFATP 516 E S R ++ + + N R V +A K + + + I +S P Sbjct: 474 EYSQKRPITTLTDHYDLIINVANVQMSTVQRIVWQATKGTPDIPFYVHEVPTIFVSVQCP 533 Query: 517 YLLQDMPFLDALINAYSLHEETVELTVKALFGEVPFQGQSPVKA 560 + L D+P + INAY E T+EL V+ L G F+G SPV A Sbjct: 534 FHLVDVPQVKTYINAYDGKEPTIELLVEKLMGNSEFKGISPVDA 577 >B0A6F5.1 B0A6F5_9CLOT Putative uncharacterized protein Length = 573 Score = 242 bits (618), Expect = 6e-62, Method: Compositional matrix adjust. Identities = 178/575 (30%), Positives = 276/575 (48%), Gaps = 43/575 (7%) Query: 7 LTREEQDWVERTLTSMSLREKIGQTMQDHAGRLPFKGNDEEALAAYLKTYPVGSFFIGGE 66 L+ E+ WVE TL +++L EKIGQ D N EE + ++ Y +G F Sbjct: 12 LSDEDIKWVENTLANLTLDEKIGQIFVDM-----LWNNTEEEVVERIQKYGMGGFRYNN- 65 Query: 67 VIQKAAGKAEEYRDWAEQLQRISKYPLLFSGDLEFGAGSAVKSLTAFPPXXXXXXXXXXX 126 A ++ R +Q SK P L + ++E G V T F Sbjct: 66 --MSPADLHKQNR----VIQENSKIPALIAANIEAGGDGGVGGGTHFGYHVTIGATQDKE 119 Query: 127 XXXXXXKFTALEGRAAGFTWALAPGTDLLLNWMNPVITTRCXXXXXXXXXXXXXXVMRGM 186 + E A G W AP D+ NW N V+++RC +RG Sbjct: 120 NAYKLGYYGCKEAAAIGCNWTFAPIVDINKNWRNCVVSSRCFSSEAQQVLDMSKEYLRGA 179 Query: 187 QDHGLAACAKHFPGDGVDYRDQHIVTTINGLSEEEWFAAYGQVSQHMIDQGVLSYMTGHI 246 +D GLA+C KHFPGDG+D RDQHIVTT N +S EEW +G+V + MID GV S M GHI Sbjct: 180 KDAGLASCMKHFPGDGLDERDQHIVTTNNDMSVEEWEENFGKVYRGMIDAGVESVMIGHI 239 Query: 247 ALP----WLEGGTQEEKPVPATVSERITTGLLRERMGFAGVVLSDALDMGGFLAWGSYEK 302 LP L G ++ + +PAT++ + LLR+ +GF G+V++DA M G + + Sbjct: 240 RLPEMSRKLRPGIKDNEIMPATIAPELLQDLLRDELGFNGLVITDATHMVGLTSAMRRSE 299 Query: 303 RMVDCFNSGTDVLLW--PGVEYFGVMEQAVSEGTVLMERLDASVRRVLELKALLGVHRVD 360 M +G D++L+ E +++ ++ G + ERL+ +V RVL KA+L +H Sbjct: 300 IMPYTIAAGCDMILYYRDKDEDVEAIKEGLANGVLTEERLNEAVTRVLAFKAMLKLHTKK 359 Query: 361 QKGRDPQAVPLLNDRLPPKLDLEARQLSRTIAERCITLVRNRGGILPLDPQTTRRVLVVL 420 +G VP + L E +Q+++ IA++ ITLV+N LPL PQT +R+++ Sbjct: 360 TEG---TLVP-AEENLSIIGCEEHKQVAKDIADQAITLVKNTKNQLPLTPQTHKRIMLYT 415 Query: 421 L-------NKVTEGRKYERMNVFVELLRARGLQVDILDEFEPLGTLRKWELSGIRWDAAF 473 + NKV R +R+ +E A+G +V + F+P L +L F Sbjct: 416 VEQAGFAPNKVEADRLNQRVKAKLE---AQGFEVTL---FDPAAGLNGKQLLAATPIKEF 469 Query: 474 APYFLPLHGMMNTARPVGEAAKAI-WAMQ-------HAETIRPIGISFATPYLLQDMPFL 525 F + ++ A + I WAM + + + +S P+ L D+P + Sbjct: 470 VDQFDAVMLFVHVAGFSQSNVRRITWAMPMGPDIPWYVTELPTVCVSVFNPFNLIDVPMV 529 Query: 526 DALINAYSLHEETVELTVKALFGEVPFQGQSPVKA 560 INAYS ++ T++L ++ + G+ F+G S V A Sbjct: 530 PTYINAYSDNDATLDLVIEKIMGKSEFKGVSSVDA 564 >Q1J2S2.1 Q1J2S2_DEIGD Glycoside hydrolase, family 3-like protein Length = 562 Score = 239 bits (610), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 201/567 (35%), Positives = 274/567 (48%), Gaps = 47/567 (8%) Query: 7 LTREEQDWVERTLTSMSLREKIGQTMQDHAGRLPFK-GNDEEALAAYLKTYPVGSFFIGG 65 L E + WV RT ++SL EK+ Q + LP G D E L +L S +GG Sbjct: 13 LNDEARAWVARTRDALSLDEKLAQLL------LPISLGLDWEELDRFL------SLGVGG 60 Query: 66 EVIQKAAGKAEEYRDWAEQLQRISKYPLLFSGDLEFGAGSAV--KSLTAFPPXXXXXXXX 123 V + A + RD A LQ S+ PLL S DLE G ++V + T +P Sbjct: 61 -VSRFATLDPQALRDSATYLQERSRIPLLLSTDLEGGELASVGGPAGTPYPNQMAVAATD 119 Query: 124 XXXXXXXXXKFTALEGRAAGFTWALAPGTDLLLNWMNPVITTRCXXXXXXXXXXXXXXVM 183 + EGRA GF +P TDL LN +NP++ TR + Sbjct: 120 DPVFARRFGQVLGREGRAVGFNCTFSPVTDLDLNPLNPIVNTRSFGSDVGRVTAFCEAAI 179 Query: 184 RGMQDHGLAACAKHFPGDGVDYRDQHIVTTINGLSEEEWFAAYGQVSQHMIDQGVLSYMT 243 RG Q G+AACAKH+PGDGVDYR+QH+VT+ N L W A+YG + +H+I GV M Sbjct: 180 RGFQAEGVAACAKHWPGDGVDYRNQHLVTSENTLDLPAWEASYGAMYRHLIASGVKIVMP 239 Query: 244 GHIALPWLEG--GTQEEKPVPATVSERITTGLLRERMGFAGVVLSDALDMGGFLAWGSYE 301 GHI LP G +E +PAT+S +TT LLR+ +GF GVV+SDA +MGGF + G E Sbjct: 240 GHITLPAYAHARGRPDEADLPATLSPLLTTTLLRDELGFGGVVVSDATEMGGFTSQGPRE 299 Query: 302 KRMVDCFNSGTDVLLWP-GVEY-FGVMEQAVSEGTVLMERLDASVRRVLELKALLGVHRV 359 + +G D+LL+P VE + + V G + R+D +V RVL LKA LG+H+ Sbjct: 300 DLVPQVIAAGCDILLFPTDVERDLEFLRRGVRNGRLSEARVDEAVTRVLALKASLGLHQS 359 Query: 360 DQKGRDPQAVPLLNDRLPPKLDLEARQLSRTIAERCITLVRNRGGILPLDPQTTRRVLVV 419 +P L P E + A R +TLV + +LPLDP RR+L++ Sbjct: 360 -------TGLPDLGTLRAP----EHLSWAEECAARAVTLVSDTQHLLPLDPARHRRLLII 408 Query: 420 LLNKVTEGRKYERMNVFV-ELLRARGLQVDILDEFEPLGTLRKWELSGIRWDAAFAPYFL 478 + G + ++ V ELL G +V + + P + +S +DA Y L Sbjct: 409 RQPQRRNGLGFPLPDLRVPELLEQAGFEVTL---YTP-----QTHVSPDLYDALL--YVL 458 Query: 479 PLHGMMNTARP----VGEAAKAIWAMQHAETIRPIGI-SFATPYLLQDMPFLDALINAYS 533 +M T V AM+ + P I S TP QD P L+NAYS Sbjct: 459 AEEALMVTPDLRLDWVELHGTPFRAMERFWDVLPTAIVSLGTPRYAQDAPRCRTLVNAYS 518 Query: 534 LHEETVELTVKALFGEVPFQGQSPVKA 560 E V+AL G+ PFQG SPV A Sbjct: 519 PVMPVQEAVVRALTGQQPFQGVSPVDA 545 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,361,687,492 Number of extensions: 135633778 Number of successful extensions: 321545 Number of sequences better than 1.0e-05: 1201 Number of HSP's gapped: 319858 Number of HSP's successfully gapped: 1202 Length of query: 577 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 436 Effective length of database: 1,732,333,088 Effective search space: 755297226368 Effective search space used: 755297226368 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)