BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0273.0008.p (158 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9E453.1 B9E453_CLOK1 Putative uncharacterized protein 230 2e-59 A5N082.1 A5N082_CLOK5 Transcriptional regulator 230 2e-59 C0GGL7.1 C0GGL7_9FIRM Transcriptional regulator, TetR family 154 3e-36 A3CPF1.1 A3CPF1_STRSV Transcriptional regulator, TetR/AcrR famil... 75 2e-12 A8AYB1.1 A8AYB1_STRGC Transcription regulator (TetR/AcrR family)... 75 2e-12 >B9E453.1 B9E453_CLOK1 Putative uncharacterized protein Length = 195 Score = 230 bits (587), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 102/158 (64%), Positives = 133/158 (84%) Query: 1 MAVGTFYNYYSSKEKLFMEIFLAENAKLKQKCMQALDLSQSPLTVVRQMMMLNMEGMTAN 60 MA GTFYNYY SK+KLFMEI+L EN KLK+ M++++L SP+ V++++M LN++GMT+N Sbjct: 35 MATGTFYNYYPSKDKLFMEIYLEENLKLKRNIMESVNLEASPIDVMKKIMFLNLKGMTSN 94 Query: 61 PILKEWYNKSVFGKIEQLFREENGTQSVDFLYDSFLEIVKQWQAEGKMRSDIDSKMIMMV 120 PILKEWYN+ VF KIEQ FREENG V FLYD+F+EIVK+WQ EGKMR+DID++MIM + Sbjct: 95 PILKEWYNRDVFSKIEQSFREENGFDCVAFLYDNFIEIVKKWQIEGKMRNDIDAEMIMAI 154 Query: 121 FTAIINVETHKEEIGPEYFPQLLDHMTELIMKGLTDCS 158 F+A++N+ETHKEEIG YFPQ+L+++ E MKGL DCS Sbjct: 155 FSALVNIETHKEEIGLRYFPQVLEYLAEFTMKGLMDCS 192 >A5N082.1 A5N082_CLOK5 Transcriptional regulator Length = 195 Score = 230 bits (587), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 102/158 (64%), Positives = 133/158 (84%) Query: 1 MAVGTFYNYYSSKEKLFMEIFLAENAKLKQKCMQALDLSQSPLTVVRQMMMLNMEGMTAN 60 MA GTFYNYY SK+KLFMEI+L EN KLK+ M++++L SP+ V++++M LN++GMT+N Sbjct: 35 MATGTFYNYYPSKDKLFMEIYLEENLKLKRNIMESVNLEASPIDVMKKIMFLNLKGMTSN 94 Query: 61 PILKEWYNKSVFGKIEQLFREENGTQSVDFLYDSFLEIVKQWQAEGKMRSDIDSKMIMMV 120 PILKEWYN+ VF KIEQ FREENG V FLYD+F+EIVK+WQ EGKMR+DID++MIM + Sbjct: 95 PILKEWYNRDVFSKIEQSFREENGFDCVAFLYDNFIEIVKKWQIEGKMRNDIDAEMIMAI 154 Query: 121 FTAIINVETHKEEIGPEYFPQLLDHMTELIMKGLTDCS 158 F+A++N+ETHKEEIG YFPQ+L+++ E MKGL DCS Sbjct: 155 FSALVNIETHKEEIGLRYFPQVLEYLAEFTMKGLMDCS 192 >C0GGL7.1 C0GGL7_9FIRM Transcriptional regulator, TetR family Length = 194 Score = 154 bits (389), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 66/156 (42%), Positives = 108/156 (69%), Gaps = 2/156 (1%) Query: 1 MAVGTFYNYYSSKEKLFMEIFLAENAKLKQKCMQALDLSQSPLTVVRQMMMLNMEGMTAN 60 +AVG+FY +Y +KE++F+++F+AE+ +LK++ M +DL P+ V+++ + N Sbjct: 35 IAVGSFYKFYGAKEEVFLDVFMAESEQLKKEIMAEVDLDDEPVAVIKEATAKMFVAIREN 94 Query: 61 PILKEWYNKSVFGKIEQLFREENGTQSVD--FLYDSFLEIVKQWQAEGKMRSDIDSKMIM 118 PIL+EWY++ V+ K+E+ E++G + + Y+ F I+K+WQ EGK R+DIDS MI+ Sbjct: 95 PILREWYSRDVYAKLEKYIYEQDGADKWEDEYSYNLFTGIIKKWQQEGKFRNDIDSDMIL 154 Query: 119 MVFTAIINVETHKEEIGPEYFPQLLDHMTELIMKGL 154 +F ++ HKEEIG +YFPQL++HM E I+KGL Sbjct: 155 AIFNTFQYIDMHKEEIGSQYFPQLMEHMVEFIVKGL 190 >A3CPF1.1 A3CPF1_STRSV Transcriptional regulator, TetR/AcrR family, putative Length = 203 Score = 75.5 bits (184), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 43/154 (27%), Positives = 80/154 (51%), Gaps = 2/154 (1%) Query: 1 MAVGTFYNYYSSKEKLFMEIFLAENAKLKQKCMQALDLSQSPLTVVRQMMMLNMEGMTAN 60 MAVG+FY YY SKE +F+E+++AEN++++++ M+ +D P +V Q+ + E ++ N Sbjct: 49 MAVGSFYKYYESKEAIFLEVYIAENSRIREETMRRVDWQGEPEAIVEQLFAVTFELISPN 108 Query: 61 PILKEWYNKSVFGKIEQLFREENGTQSVDFLYDSFLEIVKQWQAEGKMRSDIDSKMIMMV 120 IL EW + + + +++G S F ++ Q EG + I IM V Sbjct: 109 KILAEWNKPGISQILHDYYNQDSGRASNAFHQFLIQTFSQRLQEEGFSKEKIAE--IMKV 166 Query: 121 FTAIINVETHKEEIGPEYFPQLLDHMTELIMKGL 154 + I ++ H E + L+ + + +KG+ Sbjct: 167 YDLIYYIDMHVTEQEFSGYFDSLETLVKYFVKGI 200 >A8AYB1.1 A8AYB1_STRGC Transcription regulator (TetR/AcrR family) BH0580, putative Length = 188 Score = 74.7 bits (182), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 43/154 (27%), Positives = 79/154 (51%), Gaps = 2/154 (1%) Query: 1 MAVGTFYNYYSSKEKLFMEIFLAENAKLKQKCMQALDLSQSPLTVVRQMMMLNMEGMTAN 60 MAVG+FY YY SKE +F+E+++AEN++++++ M+ +D P +V Q+ + E ++ N Sbjct: 34 MAVGSFYKYYESKEAIFLEVYVAENSRIREETMRRVDWQGEPEAIVEQLFAVTFELISPN 93 Query: 61 PILKEWYNKSVFGKIEQLFREENGTQSVDFLYDSFLEIVKQWQAEGKMRSDIDSKMIMMV 120 IL EW + + + ++ G S F ++ Q EG + I IM V Sbjct: 94 KILAEWNKPGISHILHDYYNQDAGRASNAFHQFLIQTFSQRLQEEGFSKEKIAE--IMKV 151 Query: 121 FTAIINVETHKEEIGPEYFPQLLDHMTELIMKGL 154 + I ++ H E + L+ + + +KG+ Sbjct: 152 YDLIYYIDMHVTEQEFSGYFDSLETLVKYFVKGI 185 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 840,014,209 Number of extensions: 29213895 Number of successful extensions: 85116 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 85121 Number of HSP's successfully gapped: 10 Length of query: 158 Length of database: 3,060,696,203 Length adjustment: 119 Effective length of query: 39 Effective length of database: 1,939,595,418 Effective search space: 75644221302 Effective search space used: 75644221302 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)