BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0273.0002.p (295 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A5FLD7.1 A5FLD7_FLAJ1 Fructose-bisphosphate aldolase EC=4.1.2.13 424 e-117 A6LXU8.1 ALF1_CLOB8 Fructose-bisphosphate aldolase class 1 EC=4.... 419 e-115 Q97TN4.1 ALF1_CLOAB Fructose-bisphosphate aldolase class 1 EC=4.... 417 e-115 B9YBR6.1 B9YBR6_9FIRM Putative uncharacterized protein 383 e-104 C5ETW1.1 C5ETW1_9FIRM Fructose-bisphosphate aldolase class 1 382 e-104 >A5FLD7.1 A5FLD7_FLAJ1 Fructose-bisphosphate aldolase EC=4.1.2.13 Length = 298 Score = 424 bits (1091), Expect = e-117, Method: Compositional matrix adjust. Identities = 206/295 (69%), Positives = 243/295 (82%) Query: 1 MNTKQLDRIHTGKGFIAALDQSGGSTPKALLQYGIQENRYSNEEEMFELVHEMRTRIIKS 60 +N +QL+R+H+GKGFIAALDQSGGSTPKAL QYG+QEN YSN+EEM+ LVHEMRTRIIK+ Sbjct: 4 VNQEQLNRMHSGKGFIAALDQSGGSTPKALSQYGVQENSYSNDEEMYTLVHEMRTRIIKT 63 Query: 61 PAFNSRHILGAILFENTMDRSIDGQFTADYLWEQKGIVPFLKIDQGLAGPENGVQLMKPI 120 PAF+S +ILGAILFENTMDR IDG +TADYLWE+K IVPFLK+D+GLA +GVQLMKPI Sbjct: 64 PAFDSEYILGAILFENTMDRKIDGLWTADYLWEKKNIVPFLKVDKGLADLASGVQLMKPI 123 Query: 121 PGLDELLKRAVQKNIFGTKMRSLIKEANPVGXXXXXXXXXXXXXXXXXYGLVPIIEPEVD 180 P LDELL RAV++N+FGTKMRS+IKEANP G GL+PIIEPEVD Sbjct: 124 PNLDELLNRAVERNVFGTKMRSVIKEANPDGIRDIVEQQFRVGLQIFEKGLIPIIEPEVD 183 Query: 181 IHMADKGQAERLLKQEISTRLSALGKDVKVMLKLSIPTEDNFYSDLIQDPHVVRVVALSG 240 I+ ADK ++E +LK+EI +L+AL KDVKVMLKLSIPT +NFYS LI DPHVVRVVALSG Sbjct: 184 IYSADKEKSEEILKKEIQKQLNALDKDVKVMLKLSIPTVNNFYSSLISDPHVVRVVALSG 243 Query: 241 GYTQAEANDKLAHNHGLIASFSRALSQGLSDQQTDDEFNAMLAKSTEAIYEASIT 295 GY++ EAN KLA NHGLIASFSRALS+GLS Q+D +FNA+L + + IY ASIT Sbjct: 244 GYSREEANTKLAQNHGLIASFSRALSEGLSAGQSDADFNAVLGDTIKTIYNASIT 298 >A6LXU8.1 ALF1_CLOB8 Fructose-bisphosphate aldolase class 1 EC=4.1.2.13 Length = 295 Score = 419 bits (1076), Expect = e-115, Method: Compositional matrix adjust. Identities = 206/294 (70%), Positives = 236/294 (80%) Query: 1 MNTKQLDRIHTGKGFIAALDQSGGSTPKALLQYGIQENRYSNEEEMFELVHEMRTRIIKS 60 MN Q+ RIHTGKGFIAALDQSGGSTPKALL+YGI+EN YSNE+EMF LVHEMR RIIKS Sbjct: 1 MNENQMKRIHTGKGFIAALDQSGGSTPKALLEYGIKENSYSNEDEMFGLVHEMRKRIIKS 60 Query: 61 PAFNSRHILGAILFENTMDRSIDGQFTADYLWEQKGIVPFLKIDQGLAGPENGVQLMKPI 120 PAF +ILGAILFE+TM R+ID Q+T DYLW++K IVPFLK+D+GL ENGVQLMKPI Sbjct: 61 PAFTLEYILGAILFEDTMYRTIDNQYTPDYLWKEKNIVPFLKVDKGLTEIENGVQLMKPI 120 Query: 121 PGLDELLKRAVQKNIFGTKMRSLIKEANPVGXXXXXXXXXXXXXXXXXYGLVPIIEPEVD 180 LD+LLK AV+KNIFGTKMRS+IKEAN G GLVPIIEPEVD Sbjct: 121 SNLDDLLKHAVEKNIFGTKMRSVIKEANAKGIKMLVNQQFEIGKQIVEAGLVPIIEPEVD 180 Query: 181 IHMADKGQAERLLKQEISTRLSALGKDVKVMLKLSIPTEDNFYSDLIQDPHVVRVVALSG 240 IH DK ++E+LLK EI +LS L K+ KVMLKLSIPT+DNFY DLI DPHVVRVVALSG Sbjct: 181 IHSTDKEESEKLLKLEILEQLSKLDKETKVMLKLSIPTQDNFYIDLIDDPHVVRVVALSG 240 Query: 241 GYTQAEANDKLAHNHGLIASFSRALSQGLSDQQTDDEFNAMLAKSTEAIYEASI 294 GY+QAEA ++L NHGLIASFSRALSQGL+ QQT++EFN ++KS + IYEASI Sbjct: 241 GYSQAEAKERLGRNHGLIASFSRALSQGLTAQQTEEEFNGTISKSIKEIYEASI 294 >Q97TN4.1 ALF1_CLOAB Fructose-bisphosphate aldolase class 1 EC=4.1.2.13 Length = 295 Score = 417 bits (1071), Expect = e-115, Method: Compositional matrix adjust. Identities = 205/295 (69%), Positives = 236/295 (80%) Query: 1 MNTKQLDRIHTGKGFIAALDQSGGSTPKALLQYGIQENRYSNEEEMFELVHEMRTRIIKS 60 MN Q++RI KGFIAALDQSGGSTPKALLQYGI+EN YSN+EEMF LVHEMR RIIKS Sbjct: 1 MNETQMNRISKDKGFIAALDQSGGSTPKALLQYGIKENSYSNDEEMFNLVHEMRKRIIKS 60 Query: 61 PAFNSRHILGAILFENTMDRSIDGQFTADYLWEQKGIVPFLKIDQGLAGPENGVQLMKPI 120 AFNS++ILGAILFENTM R+ID ++TADYLW +K IVPFLKID+GL+ ENGVQLMKPI Sbjct: 61 TAFNSKYILGAILFENTMYRTIDHKYTADYLWNEKNIVPFLKIDKGLSELENGVQLMKPI 120 Query: 121 PGLDELLKRAVQKNIFGTKMRSLIKEANPVGXXXXXXXXXXXXXXXXXYGLVPIIEPEVD 180 LDELLK A++KNIFGTKMRS IKEAN G GLVPIIEPEVD Sbjct: 121 TNLDELLKAAIEKNIFGTKMRSFIKEANSKGIKMVVDQQFELADKIANQGLVPIIEPEVD 180 Query: 181 IHMADKGQAERLLKQEISTRLSALGKDVKVMLKLSIPTEDNFYSDLIQDPHVVRVVALSG 240 I DK ++E LLK EIS LS L K+ KVMLKLSIPT+DNFYSDL++DPHVVR+VALSG Sbjct: 181 IKSTDKEKSEELLKLEISKHLSDLDKETKVMLKLSIPTKDNFYSDLMKDPHVVRIVALSG 240 Query: 241 GYTQAEANDKLAHNHGLIASFSRALSQGLSDQQTDDEFNAMLAKSTEAIYEASIT 295 GY+Q+EAN++L+ N+GLIASFSRALS+ LS QTD+EFN L+ S + IYEASIT Sbjct: 241 GYSQSEANERLSRNNGLIASFSRALSENLSIDQTDEEFNETLSNSIKEIYEASIT 295 >B9YBR6.1 B9YBR6_9FIRM Putative uncharacterized protein Length = 295 Score = 383 bits (983), Expect = e-104, Method: Compositional matrix adjust. Identities = 190/294 (64%), Positives = 227/294 (77%) Query: 1 MNTKQLDRIHTGKGFIAALDQSGGSTPKALLQYGIQENRYSNEEEMFELVHEMRTRIIKS 60 MN +QL+R+H+ KGFIAALDQSGGSTPKAL YGI+E++Y+ EE MF+L+H+MR+RII S Sbjct: 1 MNQEQLNRMHSAKGFIAALDQSGGSTPKALRLYGIEEDQYNGEEAMFDLIHQMRSRIITS 60 Query: 61 PAFNSRHILGAILFENTMDRSIDGQFTADYLWEQKGIVPFLKIDQGLAGPENGVQLMKPI 120 PAF S HIL AILFE TMDR I+GQ+TADYLWE+KGI+P LK+D+GLA EN VQLMKPI Sbjct: 61 PAFTSEHILAAILFEMTMDRQIEGQYTADYLWEKKGILPILKVDKGLAPLENDVQLMKPI 120 Query: 121 PGLDELLKRAVQKNIFGTKMRSLIKEANPVGXXXXXXXXXXXXXXXXXYGLVPIIEPEVD 180 L LL+RA Q++IFGTKMRS+IK ANP G G VPI+EPEVD Sbjct: 121 GNLKGLLERAGQRHIFGTKMRSVIKGANPDGIRAIVDQQFEIGLEIAKAGFVPILEPEVD 180 Query: 181 IHMADKGQAERLLKQEISTRLSALGKDVKVMLKLSIPTEDNFYSDLIQDPHVVRVVALSG 240 I + DK + E LLK EI L L DV++M KLSIPT D FYSDL+ DPHVVRVVALSG Sbjct: 181 ITLPDKEKCEILLKAEIERHLKTLEADVRLMFKLSIPTLDGFYSDLMNDPHVVRVVALSG 240 Query: 241 GYTQAEANDKLAHNHGLIASFSRALSQGLSDQQTDDEFNAMLAKSTEAIYEASI 294 GY++ EAN+KLA N GLIASFSRAL++GL QQTDDEFNA+L +S + IY+ASI Sbjct: 241 GYSRKEANEKLARNPGLIASFSRALTEGLKAQQTDDEFNALLQESIQTIYDASI 294 >C5ETW1.1 C5ETW1_9FIRM Fructose-bisphosphate aldolase class 1 Length = 295 Score = 382 bits (980), Expect = e-104, Method: Compositional matrix adjust. Identities = 183/295 (62%), Positives = 230/295 (77%) Query: 1 MNTKQLDRIHTGKGFIAALDQSGGSTPKALLQYGIQENRYSNEEEMFELVHEMRTRIIKS 60 MN QL R+ GKGFIAALDQSGGSTPKAL YGI E+RYS +EEMF LVHEMRTRII S Sbjct: 1 MNQDQLKRMSEGKGFIAALDQSGGSTPKALKNYGINEDRYSTDEEMFNLVHEMRTRIITS 60 Query: 61 PAFNSRHILGAILFENTMDRSIDGQFTADYLWEQKGIVPFLKIDQGLAGPENGVQLMKPI 120 P+F S+HIL AILFENTM+R ID + TADYLWE KGI+P LK+D+GLA E+GV+ MKPI Sbjct: 61 PSFTSQHILAAILFENTMERRIDDRLTADYLWEVKGIIPILKVDKGLAEEEDGVRPMKPI 120 Query: 121 PGLDELLKRAVQKNIFGTKMRSLIKEANPVGXXXXXXXXXXXXXXXXXYGLVPIIEPEVD 180 PGLD+LL RA +++IFGTKMRS+IK+ANP G GLVPI+EPE+D Sbjct: 121 PGLDDLLARAKERHIFGTKMRSVIKKANPEGIKKIIDQQFEIGLKIAQAGLVPILEPEID 180 Query: 181 IHMADKGQAERLLKQEISTRLSALGKDVKVMLKLSIPTEDNFYSDLIQDPHVVRVVALSG 240 I+ +DK + ER++K+E+ L+ L +D ++M KLSIP + FYSD+++DPHV+RVVALSG Sbjct: 181 IYSSDKAECERIMKEEMKKHLAELPQDTRIMFKLSIPDKHGFYSDIMEDPHVIRVVALSG 240 Query: 241 GYTQAEANDKLAHNHGLIASFSRALSQGLSDQQTDDEFNAMLAKSTEAIYEASIT 295 GY++ EAN++L+ + GLIASFSRALS+GL+ QT EF+ MLA+S + IYEASIT Sbjct: 241 GYSRQEANERLSRSPGLIASFSRALSEGLNVDQTAGEFDRMLAQSIKEIYEASIT 295 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,589,879,503 Number of extensions: 60582691 Number of successful extensions: 153009 Number of sequences better than 1.0e-05: 168 Number of HSP's gapped: 152863 Number of HSP's successfully gapped: 169 Length of query: 295 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 161 Effective length of database: 1,798,280,193 Effective search space: 289523111073 Effective search space used: 289523111073 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)