BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0273.0001.p (369 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C2PHL1.1 C2PHL1_BACCE Pyruvate kinase 375 e-102 Q734Q2.1 Q734Q2_BACC1 Pyruvate kinase EC=2.7.1.40 375 e-102 C1ELJ2.1 C1ELJ2_BACC3 Pyruvate kinase EC=2.7.1.40 373 e-101 C2MN22.1 C2MN22_BACCE Pyruvate kinase 372 e-101 B9ISJ4.1 B9ISJ4_BACCQ Pyruvate kinase EC=2.7.1.40 372 e-101 >C2PHL1.1 C2PHL1_BACCE Pyruvate kinase Length = 356 Score = 375 bits (963), Expect = e-102, Method: Compositional matrix adjust. Identities = 181/340 (53%), Positives = 246/340 (72%), Gaps = 5/340 (1%) Query: 1 MSIDLICTIGPASSSPAVLKELLRGGMTTARLNMSHGSHEEHGXXXXXXXXXXXXTGQPV 60 M+ID +CTIGPAS++ L +L+ GM RLN+SHG+HE H + Sbjct: 5 MTIDRVCTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESH----KDIIRLVKSLDDSI 60 Query: 61 RIMGDLQGPKIRLKSIQGDAVTLEEGQTFILDQSDEPGSRERAALDNPGVMEDILQGAAI 120 +I+GD+QGPKIRL ++G+ +TL+ G +FIL GS A++D G+ D+ G+ I Sbjct: 61 KILGDVQGPKIRLGEVKGEQITLQAGDSFILHMQPVTGSNREASVDYEGIANDVKVGSRI 120 Query: 121 LINDGEVKLEVTDKHPQRITTRVIVGGMIGSRKGVNLPGTLIRLPAITEKDKQDLQFLLE 180 LINDGEV+L V +I T+V GG I S KGVNLPG ++RLPAITEKDK+D+QFLLE Sbjct: 121 LINDGEVELIVETVSADKIETKVKTGGNISSHKGVNLPGAIVRLPAITEKDKKDIQFLLE 180 Query: 181 HHVDWIACSFIREASHLQEIRDYVSLLGKYSQPGLISKIETIQAVRNFPAIMEASDGIMI 240 VD+IACSF+R+ SH++EIRDY+ K + P LI+KIET++A+ NF I + +DGIMI Sbjct: 181 EDVDFIACSFVRKPSHIKEIRDYIQQY-KETSPNLIAKIETMEAIENFQDICKEADGIMI 239 Query: 241 ARGDLGVELPFERIPFIQKTVLRECSLSKTYVITATQMLQSMVEHPVPTRAEVTDVSQAV 300 ARGDLGVELP++ IP +QK +++EC+ + TYVITATQMLQSMV+H +PTRAEVTDV QAV Sbjct: 240 ARGDLGVELPYQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAV 299 Query: 301 LDGSDGVMLSAESSVGHYPVRSTQVLNTVAQFAENMREQG 340 LDG++ VMLSAES+ G +P+ S + L V++FAE++++ G Sbjct: 300 LDGTNAVMLSAESASGEHPIESVKTLRLVSEFAEHVKKDG 339 >Q734Q2.1 Q734Q2_BACC1 Pyruvate kinase EC=2.7.1.40 Length = 352 Score = 375 bits (962), Expect = e-102, Method: Compositional matrix adjust. Identities = 182/340 (53%), Positives = 247/340 (72%), Gaps = 5/340 (1%) Query: 1 MSIDLICTIGPASSSPAVLKELLRGGMTTARLNMSHGSHEEHGXXXXXXXXXXXXTGQPV 60 M+ID ICTIGPAS++ L +L+ GM RLN+SHG+HE H + Sbjct: 1 MTIDRICTIGPASNNKETLAQLISNGMKIVRLNLSHGTHESH----KDIIRLVKSLDDSI 56 Query: 61 RIMGDLQGPKIRLKSIQGDAVTLEEGQTFILDQSDEPGSRERAALDNPGVMEDILQGAAI 120 +I+GD+QGPKIRL ++G+ +TL+ G +FIL GS A++D G+ D+ G+ I Sbjct: 57 KILGDVQGPKIRLGEVKGEQITLQAGDSFILHTQPVTGSSTEASVDYEGIANDVKVGSRI 116 Query: 121 LINDGEVKLEVTDKHPQRITTRVIVGGMIGSRKGVNLPGTLIRLPAITEKDKQDLQFLLE 180 L+NDGEV+L V +I T+V GG I S KGVNLPG ++RLPAITEKDKQD+QFLLE Sbjct: 117 LMNDGEVELIVGKVGKDKIETKVKAGGNISSHKGVNLPGAIVRLPAITEKDKQDIQFLLE 176 Query: 181 HHVDWIACSFIREASHLQEIRDYVSLLGKYSQPGLISKIETIQAVRNFPAIMEASDGIMI 240 VD+IACSF+R+ SH++EIRD++ K + P LI+KIET++A++NF I + +DGIMI Sbjct: 177 EGVDFIACSFVRKPSHIKEIRDFIQQY-KETSPNLIAKIETMEAIKNFXDICKEADGIMI 235 Query: 241 ARGDLGVELPFERIPFIQKTVLRECSLSKTYVITATQMLQSMVEHPVPTRAEVTDVSQAV 300 ARGDLGVELPF+ +P +QK +++EC+ + TYVITATQMLQSMVEH +PTRAEVTDV QAV Sbjct: 236 ARGDLGVELPFQFVPLLQKMMIQECNRTNTYVITATQMLQSMVEHSIPTRAEVTDVFQAV 295 Query: 301 LDGSDGVMLSAESSVGHYPVRSTQVLNTVAQFAENMREQG 340 LDG++ VMLSAES+ G +P+ S + L V++FAE++++ G Sbjct: 296 LDGTNAVMLSAESASGEHPIESVKTLRLVSEFAEHVKKDG 335 >C1ELJ2.1 C1ELJ2_BACC3 Pyruvate kinase EC=2.7.1.40 Length = 353 Score = 373 bits (957), Expect = e-101, Method: Compositional matrix adjust. Identities = 180/340 (52%), Positives = 248/340 (72%), Gaps = 5/340 (1%) Query: 1 MSIDLICTIGPASSSPAVLKELLRGGMTTARLNMSHGSHEEHGXXXXXXXXXXXXTGQPV 60 M+ID ICTIGPAS++ L +L+ GM RLN+SHG+HE H + Sbjct: 1 MTIDRICTIGPASNNKETLAQLISNGMKIVRLNLSHGTHESH----KDIIRLVKSLDDSI 56 Query: 61 RIMGDLQGPKIRLKSIQGDAVTLEEGQTFILDQSDEPGSRERAALDNPGVMEDILQGAAI 120 +I+GD+QGPKIRL ++G+ +TL+ G +F+L GS + A++D G+ D+ G+ I Sbjct: 57 KILGDVQGPKIRLGEVKGEQITLQAGDSFMLHTQPVTGSNKEASVDYEGIANDVKVGSRI 116 Query: 121 LINDGEVKLEVTDKHPQRITTRVIVGGMIGSRKGVNLPGTLIRLPAITEKDKQDLQFLLE 180 L+NDGEV+L V +I T+V GG I S KGVNLPG ++RLPAITEKDK+D+QFLLE Sbjct: 117 LMNDGEVELIVEKISAGKIETKVKTGGNISSHKGVNLPGAIVRLPAITEKDKKDIQFLLE 176 Query: 181 HHVDWIACSFIREASHLQEIRDYVSLLGKYSQPGLISKIETIQAVRNFPAIMEASDGIMI 240 +VD+IACSF+R+A H++EIRD++ K S P LI+KIET++A+ NF I + +DGIMI Sbjct: 177 ENVDFIACSFVRKAGHIKEIRDFIQQ-HKASSPNLIAKIETMEAIENFQDICKEADGIMI 235 Query: 241 ARGDLGVELPFERIPFIQKTVLRECSLSKTYVITATQMLQSMVEHPVPTRAEVTDVSQAV 300 ARGDLGVELP++ IP +QK +++EC+ + TYVITATQMLQSMV+H +PTRAEVTDV QAV Sbjct: 236 ARGDLGVELPYQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAV 295 Query: 301 LDGSDGVMLSAESSVGHYPVRSTQVLNTVAQFAENMREQG 340 LDG++ VMLSAES+ G +P+ S + L V++FAE++++ G Sbjct: 296 LDGTNAVMLSAESASGEHPIESVKTLRLVSEFAEHVKKDG 335 >C2MN22.1 C2MN22_BACCE Pyruvate kinase Length = 356 Score = 372 bits (956), Expect = e-101, Method: Compositional matrix adjust. Identities = 182/340 (53%), Positives = 245/340 (72%), Gaps = 5/340 (1%) Query: 1 MSIDLICTIGPASSSPAVLKELLRGGMTTARLNMSHGSHEEHGXXXXXXXXXXXXTGQPV 60 M+ID ICTIGPAS++ L +L+ GM RLN+SHG+HE H + Sbjct: 5 MTIDRICTIGPASNNKETLAQLISNGMKIVRLNLSHGTHESH----KDIIRLVKSLDDSI 60 Query: 61 RIMGDLQGPKIRLKSIQGDAVTLEEGQTFILDQSDEPGSRERAALDNPGVMEDILQGAAI 120 +I+GD+QGPKIRL ++G+ +TL+ G +FIL GS A++D G+ D+ G+ I Sbjct: 61 KILGDVQGPKIRLGEMKGEQITLQAGDSFILHTQPVTGSSTEASVDYEGIANDVKVGSRI 120 Query: 121 LINDGEVKLEVTDKHPQRITTRVIVGGMIGSRKGVNLPGTLIRLPAITEKDKQDLQFLLE 180 L+NDGEV+L V +I T+V GG I S KGVNLPG ++RLPAITEKDK+D+QFLLE Sbjct: 121 LVNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVRLPAITEKDKKDIQFLLE 180 Query: 181 HHVDWIACSFIREASHLQEIRDYVSLLGKYSQPGLISKIETIQAVRNFPAIMEASDGIMI 240 VD+IACSF+R+ SH++EIRD++ K + P LI+KIET++A+ NF I + +DGIMI Sbjct: 181 EGVDFIACSFVRKPSHIKEIRDFIQQY-KETSPNLIAKIETMEAIENFQDICKEADGIMI 239 Query: 241 ARGDLGVELPFERIPFIQKTVLRECSLSKTYVITATQMLQSMVEHPVPTRAEVTDVSQAV 300 ARGDLGVELPF+ IP +QK +++EC+ + TYVITATQMLQSMV+H +PTRAEVTDV QAV Sbjct: 240 ARGDLGVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAV 299 Query: 301 LDGSDGVMLSAESSVGHYPVRSTQVLNTVAQFAENMREQG 340 LDG++ VMLSAES+ G +PV S L V++FAE++++ G Sbjct: 300 LDGTNAVMLSAESASGEHPVESVSTLRLVSEFAEHVKKDG 339 >B9ISJ4.1 B9ISJ4_BACCQ Pyruvate kinase EC=2.7.1.40 Length = 352 Score = 372 bits (954), Expect = e-101, Method: Compositional matrix adjust. Identities = 182/340 (53%), Positives = 245/340 (72%), Gaps = 5/340 (1%) Query: 1 MSIDLICTIGPASSSPAVLKELLRGGMTTARLNMSHGSHEEHGXXXXXXXXXXXXTGQPV 60 M+ID ICTIGPAS++ L +L+ GM RLN+SHG+HE H + Sbjct: 1 MTIDRICTIGPASNNKETLAQLISNGMKIVRLNLSHGTHESH----KDIIRLVKSLDDSI 56 Query: 61 RIMGDLQGPKIRLKSIQGDAVTLEEGQTFILDQSDEPGSRERAALDNPGVMEDILQGAAI 120 +I+GD+QGPKIRL ++G+ +TL+ G +FIL GS A++D G+ D+ G+ I Sbjct: 57 KILGDVQGPKIRLGEMKGEQITLQAGDSFILHTQPVTGSSTEASVDYEGIANDVKVGSRI 116 Query: 121 LINDGEVKLEVTDKHPQRITTRVIVGGMIGSRKGVNLPGTLIRLPAITEKDKQDLQFLLE 180 L+NDGEV+L V +I T+V GG I S KGVNLPG ++RLPAITEKDK+D+QFLLE Sbjct: 117 LMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVRLPAITEKDKKDIQFLLE 176 Query: 181 HHVDWIACSFIREASHLQEIRDYVSLLGKYSQPGLISKIETIQAVRNFPAIMEASDGIMI 240 VD+IACSF+R+ SH++EIRD++ K + P LI+KIET++A+ NF I + +DGIMI Sbjct: 177 EGVDFIACSFVRKPSHIKEIRDFIQQY-KETSPNLIAKIETMEAIENFQDICKEADGIMI 235 Query: 241 ARGDLGVELPFERIPFIQKTVLRECSLSKTYVITATQMLQSMVEHPVPTRAEVTDVSQAV 300 ARGDLGVELPF+ IP +QK +++EC+ + TYVITATQMLQSMV+H +PTRAEVTDV QAV Sbjct: 236 ARGDLGVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAV 295 Query: 301 LDGSDGVMLSAESSVGHYPVRSTQVLNTVAQFAENMREQG 340 LDG++ VMLSAES+ G +PV S L V++FAE++++ G Sbjct: 296 LDGTNAVMLSAESASGEHPVESVSTLRLVSEFAEHVKKDG 335 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,953,002,942 Number of extensions: 73670710 Number of successful extensions: 191383 Number of sequences better than 1.0e-05: 2594 Number of HSP's gapped: 185679 Number of HSP's successfully gapped: 2623 Length of query: 369 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 232 Effective length of database: 1,770,017,148 Effective search space: 410643978336 Effective search space used: 410643978336 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)