BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0272.0005.p (473 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K643.1 B3K643_9BACI Extracellular solute-binding protein family 1 729 0.0 B1D783.1 B1D783_9BACL Extracellular solute-binding protein family 1 331 1e-88 A4U8T8.1 A4U8T8_9BACT Sugar permease 272 4e-71 Q5WIQ4.1 Q5WIQ4_BACSK Sugar ABC transporter substrate-binding pr... 258 1e-66 A9KIY0.1 A9KIY0_CLOPH Extracellular solute-binding protein family 1 211 1e-52 >B3K643.1 B3K643_9BACI Extracellular solute-binding protein family 1 Length = 468 Score = 729 bits (1882), Expect = 0.0, Method: Compositional matrix adjust. Identities = 342/388 (88%), Positives = 370/388 (95%) Query: 55 GSAGGDKVTLKFTFWGSPQEKQAVEDAIKAFEAKNPNITIDSIHIPGTDFLQKLNAMIAG 114 G+ G+KVTLKFTFWGSP+EK+AVE AIKAFE KNPNITID +HIPGTDFLQKLNAMIAG Sbjct: 45 GTDNGEKVTLKFTFWGSPEEKKAVESAIKAFEEKNPNITIDPVHIPGTDFLQKLNAMIAG 104 Query: 115 NEAPDLSYSAAWKLKMGEEGLIYNFFDLMKDDPSIKKEDYLQYAWWNWDTDKSAGPYQAS 174 NEAPD+SYSAAWKLKMGEEGLIYNFFDLMKDDPSIKKEDYL+YAWWNW+ DKSAGPYQAS Sbjct: 105 NEAPDISYSAAWKLKMGEEGLIYNFFDLMKDDPSIKKEDYLEYAWWNWEQDKSAGPYQAS 164 Query: 175 VVPSLMYNADLFKEAGVELPPTKAEEAWQWDEFVDTAKKLTLDRNGKHPDDPGFDPKNIK 234 VVPSLMYNAD+F+EAGV LPPTKAEEAW W++FVD AKKLTLDRNGKHPDDPGFDPKNIK Sbjct: 165 VVPSLMYNADMFQEAGVALPPTKAEEAWTWEQFVDAAKKLTLDRNGKHPDDPGFDPKNIK 224 Query: 235 QYGVKFSLSWLSYMPLVLSNGGDYLTKDGKEFGLSKPEATEAIQRIADLINVYHVAPSPV 294 QYGVK +L W SYMP+VLSNGGDYL+ DGKEFGLSKPEATEA+QRIADLINV+HV+PSPV Sbjct: 225 QYGVKLNLGWSSYMPMVLSNGGDYLSADGKEFGLSKPEATEALQRIADLINVHHVSPSPV 284 Query: 295 QASSIPAPATALQSRKVAMVIDGSWNHLDLSKANINWGVGVLPVLKDYKTFFLGGSLIIF 354 QASSIPAPATALQS+KVAM+IDGSWNHLDLSK NINWGVGVLPV+KDYKTFFLGGSLIIF Sbjct: 285 QASSIPAPATALQSKKVAMIIDGSWNHLDLSKTNINWGVGVLPVIKDYKTFFLGGSLIIF 344 Query: 355 KSSKHPKEAWEFSKFLTDPQNVLELHQGLWMPQLKQWYEDPKLVDSWANEKLPGRPAGFQ 414 KSSKHPKE WEFSKFLTDPQNVL+LHQGLWMPQL +WYEDP+L++ WANE+LPGRPAGFQ Sbjct: 345 KSSKHPKETWEFSKFLTDPQNVLDLHQGLWMPQLNKWYEDPELIEEWANEELPGRPAGFQ 404 Query: 415 DAVMRSTYEHAEPSAENNVRNFVEIDAA 442 DAVMRSTYEHAEPS ENNV+NFVEIDAA Sbjct: 405 DAVMRSTYEHAEPSPENNVKNFVEIDAA 432 >B1D783.1 B1D783_9BACL Extracellular solute-binding protein family 1 Length = 483 Score = 331 bits (848), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 169/390 (43%), Positives = 234/390 (60%), Gaps = 4/390 (1%) Query: 53 ETGSAGGDKVTLKFTFWGSPQEKQAVEDAIKAFEAKNPNITIDSIHIPGTDFLQKLNAMI 112 ++G+ G+K+TLK T WG+P E A + AI FE +PN+ +D I D+ KL M+ Sbjct: 55 DSGTTSGEKITLKLTGWGAPAEVAAYKSAIANFEKTHPNVKVDFQAISSGDYDTKLTTMV 114 Query: 113 AGNEAPDLSY--SAAWKLKMGEEGLIYNFFDLMKDDPSIKKEDYLQYAWWNWDTDKSAGP 170 AGN+ PD++ SA + EEG N D + D I + + ++ G Sbjct: 115 AGNDEPDVAMMESATIAFPLAEEGKFLNLQDFLAKDQQITLDSLVPNITYSQAPGNIIGI 174 Query: 171 YQASVVPSLMYNADLFKEAGVELPPTKAEEAWQWDEFVDTAKKLTLDRNGKHPDDPGFDP 230 L YN D+FK+AG+E PP+ +AW WD+FVD AKKLTLD G++ DP FDP Sbjct: 175 GPGPETFGLFYNEDVFKDAGIEPPPSDVSKAWNWDQFVDVAKKLTLDNKGRNATDPSFDP 234 Query: 231 KNIKQYGVKFSLSWLSYMPLVLSNGGDYLTKDGKEFGLSKPEATEAIQRIADLINVYHVA 290 KNIKQ+G+ W +Y + SNGGD+L+ DGK F L++PEA E Q++ADLINVYHVA Sbjct: 235 KNIKQFGINLPTWWGAYSNFIYSNGGDFLSADGKTFALNQPEAVEVFQKMADLINVYHVA 294 Query: 291 PSPVQASSIPAPATALQSRKVAMVIDGSWNHLDLSKANINWGVGVLPVLKDYKTFFLGGS 350 PSP+Q+ +IP ALQ++KVAM IDG W L+++ N+ VGVLPV+K+ T + G Sbjct: 295 PSPLQSKNIPGTNVALQTKKVAMAIDGQWASSSLAESKFNYNVGVLPVMKEPVTTVVAGM 354 Query: 351 LIIFKSSKHPKEAWEFSKFLTDPQNVL-ELHQGLWMPQLKQWYEDPKLVDSWANEKLPGR 409 IFKS+KHP+E+WE K L DP+ L L G WMP K WY D + +D W + L R Sbjct: 355 FSIFKSTKHPQESWELLKALLDPEASLGMLTNGTWMPAPKDWYTDSEKLDKWTKD-LAAR 413 Query: 410 PAGFQDAVMRSTYEHAEPSAENNVRNFVEI 439 P+G+ AV+ H+ + V+NF +I Sbjct: 414 PSGYNGAVIDMLLNHSHATPTAYVKNFNKI 443 >A4U8T8.1 A4U8T8_9BACT Sugar permease Length = 450 Score = 272 bits (696), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 141/350 (40%), Positives = 196/350 (56%), Gaps = 5/350 (1%) Query: 61 KVTLKFTFWGSPQEKQAVEDAIKAFEAKNPNITIDSIHIPGTDFLQKLNAMIAGNEAPDL 120 +V L FTFWG E+ AV ++ F + +PNI + HIP + +K++ M AG PD Sbjct: 33 QVELTFTFWGGTFERDAVNQTLETFNSLHPNIHVTGQHIPNAGYNEKISTMAAGGTLPDA 92 Query: 121 SY-SAAWKLKMGEEGLIYNFFDLMKDDPSIKKEDYLQYAWWNWDT-DKSAGPYQASVVPS 178 Y ++ L+ +GLI + DP D L+ W+ WD K+ G A Sbjct: 93 GYMDSSMALRWATDGLIKDLTTQFASDP--MAADRLENMWYRWDNGTKTIGTAAAVESMI 150 Query: 179 LMYNADLFKEAGVELPPTKAEEAWQWDEFVDTAKKLTLDRNGKHPDDPGFDPKNIKQYGV 238 L YN DLF AG++ PP A EAW WDEFVD AK+LT+DR G + P FDP NI +G+ Sbjct: 151 LYYNRDLFDAAGIDYPPEFASEAWTWDEFVDIAKQLTVDRQGNNAASPDFDPDNIDVFGI 210 Query: 239 KFSLSWLSYMPLVLSNGGDYLTKDGKEFGLSKPEATEAIQRIADLINVYHVAPSPVQASS 298 F W ++P V SNGG + DG E +++PEA EA+Q + DLI V+HVAP+P Q+ S Sbjct: 211 AFPTWWAGWLPFVYSNGGQLASDDGTELLINRPEAVEALQALQDLIYVHHVAPTPAQSQS 270 Query: 299 IPAPATALQSRKVAMVIDGSWNHLDLSKANINWGVGVLPVLKDYKTFFLGGSLIIFKSSK 358 +PA +Q KVAM +DG W L +S+ NW GVLP+ + T GG +I + ++ Sbjct: 271 LPAADIMMQLGKVAMSVDGHWRTLAMSQLGFNWSFGVLPMHQRPATLIWGGVTVILEGTE 330 Query: 359 HPKEAWEFSKFLTDPQNVLELHQGLWMPQLKQWYEDPKLVDSWANEKLPG 408 HP EA+E FL+DP +GLWMP + +Y D W + +PG Sbjct: 331 HPDEAFELINFLSDPTQSALFTRGLWMPLQRIYYTDEAKAAEWL-DAIPG 379 >Q5WIQ4.1 Q5WIQ4_BACSK Sugar ABC transporter substrate-binding protein Length = 452 Score = 258 bits (659), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 139/368 (37%), Positives = 217/368 (58%), Gaps = 16/368 (4%) Query: 60 DKVTLKFTFWGSPQEKQAVEDAIKAFEAKNPNITIDSIHIPGTDFLQKLNAMIAGNEAPD 119 D V L+FT+WG P E++AVE I F ++P I + + +P +++K+N M A PD Sbjct: 32 DPVELRFTYWGGPLEREAVETLISTFNNEHPGIQVRAQLVPINSYVEKMNVMAASKTLPD 91 Query: 120 LSYSAAWKL-KMGEEGLIYNFFDLMKDDPSIKKEDYLQYAWWNWDTDKSAGPYQASVVPS 178 + Y L E G++ + L+ + + +K DY Y + + AG A+ V + Sbjct: 92 VGYFPEGSLYPWVENGMLRDLTPLLTGNEANEKLDYTLYQFHD---GPIAGGSVANEVIN 148 Query: 179 LMYNADLFKEAGVELPPTKAEEAWQWDEFVDTAKKLTLDRNGKHPDDPGFDPKNIKQYGV 238 + YN LF EAGVELPPTKAEEAW WDEFV+ AK+LT+DRNG+ ++PGFD I+ YGV Sbjct: 149 IYYNKQLFAEAGVELPPTKAEEAWSWDEFVEVAKRLTVDRNGRRANEPGFDENRIETYGV 208 Query: 239 KFSLSWLSYMPLVLSNGGDYLTKDGKEFGLSKPEATEAIQRIADLINVYHVAPSPVQASS 298 W+ + SNGG ++ + GK+ + + E+ E +Q +ADL+ V+HV P P S+ Sbjct: 209 SNFTGWID--SFLFSNGGGFVDETGKQLLIDQSESIEVLQHVADLMYVHHVMPKPSDLST 266 Query: 299 IPAPATALQSRKVAMVIDGSWNHLDLSKANI----NWGVGVLPVLKDYKTFFLGGSLIIF 354 +PA TAL +++VAM +DG W +L +A++ ++G+GVLP + T G ++ F Sbjct: 267 VPATDTALLTKRVAMAVDGQWAMQELGRASVEDGLDFGIGVLPYFEQPATTNYGTPIVAF 326 Query: 355 KSSK---HPKEAWEFSKFLTDPQNVLEL-HQGLWMPQLKQWYEDPKLVDSWANEKLPGRP 410 ++ K H + F ++ DP+NVL L ++GLWMP WYED + ++ W + P P Sbjct: 327 ETGKEGEHRQAVDTFLTYIMDPENVLMLINEGLWMPNETAWYEDREKIERWID--TPIHP 384 Query: 411 AGFQDAVM 418 +++AV+ Sbjct: 385 PEYEEAVI 392 >A9KIY0.1 A9KIY0_CLOPH Extracellular solute-binding protein family 1 Length = 456 Score = 211 bits (537), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 137/397 (34%), Positives = 206/397 (51%), Gaps = 16/397 (4%) Query: 52 GETGSAGGDKVTLKFTFWGSPQEKQAVEDAIKAFEAKNPNITIDSIHIPGTDFLQKLNAM 111 G S+ KV + ++ WGS E + F A I +D + IP +++ KLN M Sbjct: 31 GNGKSSKDGKVKITYSMWGSADEGAVTQKLADKFNASQDRIEVDVLAIPWENYMTKLNTM 90 Query: 112 IAGNEAPDLS-YSAAWKLKMGEEGLIYNFFDLMKDDPSIKKEDYLQYAWWNWDTDKSAGP 170 E PD S A L+ E+G++ + + + S K D L + + K+ Sbjct: 91 ATAKELPDTGIMSEAGVLQWAEQGMLADISTMYGKNDS-KPLDSLAFRY----QGKTVAY 145 Query: 171 YQASVVPSLMYNADLFKEAGVELPPTKAEEAWQWDEFVDTAKKLTLDRNGKHPDDPGFDP 230 A+ V L YN D+F +AG PP + AW W+EFV TAKKLTLD+NGKHPD+ GFD Sbjct: 146 SAANEVLLLYYNKDMFDKAGATYPPASVDNAWTWEEFVSTAKKLTLDKNGKHPDEKGFDE 205 Query: 231 KNIKQYGVKF-SLSW-LSYMPLVLSNGGDYLTKDGKEFGLSKPEATEAIQRIADLINVYH 288 +NI QYG +L+W L P +SNG Y + DG E + + EAIQ++ADL V H Sbjct: 206 QNIVQYGCLVENLAWQLELWP--MSNGAGYYSSDGSEVTIDNQASIEAIQKVADLYLVDH 263 Query: 289 VAPSPVQASSIPAPATALQSRKVAMVIDGSWN-HLDLSKAN---INWGVGVLPVLKDYKT 344 VAP A+ + ++ S AM G+WN L+ A +N+GV VLP +K+ T Sbjct: 264 VAPLSTGATD-DSIQRSIISGTCAMGTGGAWNVGTCLASAREEGLNYGVAVLPYMKEKLT 322 Query: 345 FFLGGSLIIFKSSKHPKEAWEFSKFLTDPQNVLEL-HQGLWMPQLKQWYEDPKLVDSWAN 403 GG ++F +KHP+EA E+ K+ +N L G+WMP L++WY D + W + Sbjct: 323 LCTGGPNVVFSQTKHPEEAMEWLKWYYQEENSWSLIETGIWMPILQKWYTDETMTHKWVD 382 Query: 404 EKLPGRPAGFQDAVMRSTYEHAEPSAENNVRNFVEID 440 K ++ AV+ ++++ ++ V N V+ + Sbjct: 383 NKNFPPYEEYKSAVVDYAMKNSKSASWYYVNNTVDFN 419 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.132 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,849,780,957 Number of extensions: 126473899 Number of successful extensions: 218333 Number of sequences better than 1.0e-05: 703 Number of HSP's gapped: 217802 Number of HSP's successfully gapped: 705 Length of query: 473 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 333 Effective length of database: 1,741,754,103 Effective search space: 580004116299 Effective search space used: 580004116299 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)