BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0272.0002.p (315 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B2IAY7.1 B2IAY7_BEII9 3-beta hydroxysteroid dehydrogenase/isomerase 438 e-121 A9U7S2.1 A9U7S2_PHYPA Predicted protein (Fragment) 254 8e-66 Q095J3.1 Q095J3_STIAU NAD(P)-dependent steroid dehydrogenase 196 3e-48 Q1D036.1 Q1D036_MYXXD NAD dependent epimerase/dehydratase family... 166 3e-39 A7HT92.1 A7HT92_PARL1 3-beta hydroxysteroid dehydrogenase/isomerase 158 7e-37 >B2IAY7.1 B2IAY7_BEII9 3-beta hydroxysteroid dehydrogenase/isomerase Length = 330 Score = 438 bits (1127), Expect = e-121, Method: Compositional matrix adjust. Identities = 212/275 (77%), Positives = 234/275 (85%) Query: 1 MGATPVDADLESSMPFVLPMIDAVVHAAALFRFSGPREPFFRTNVDGTXXXXXXXXXXXX 60 +GA+PVDADLESS P LP IDAVVHAAALFRF+GPREPFFRTNVDGT Sbjct: 43 LGASPVDADLESSAPLALPAIDAVVHAAALFRFAGPREPFFRTNVDGTVALLKATEKAGA 102 Query: 61 XTFVYISAAGIHMDNSGTLIRDADESAPTFPNHFSAYLASKARADSLVLAANKPGFRTIA 120 TFVYISAAGI MD++GT IR ADESAP FPNHFSAYLASKARA+ LVLAANKPGFRTIA Sbjct: 103 QTFVYISAAGIIMDDAGTPIRGADESAPIFPNHFSAYLASKARAEPLVLAANKPGFRTIA 162 Query: 121 LRPPAIWGPGDPFSRALPEAVRSGQFAFIDHGDYPFSTCHVDNVVEAIQCALERGEGGHA 180 LRPPAIWGPGD FSR LP ++SGQFAFID GDY F+TCHVDNVVEA+QCALE GEGG A Sbjct: 163 LRPPAIWGPGDSFSRELPRVIKSGQFAFIDRGDYAFATCHVDNVVEAVQCALECGEGGRA 222 Query: 181 FFIKDQEGQTFREFAASLANLQGLSIDKMRSMSYWLVSAIGRLFDTIWAVTRKDGDPPIS 240 FFI DQE TFR+F ASLA+LQGLSIDK+RSM YWL SA+GR+ D IWA+TR++G+PPIS Sbjct: 223 FFINDQERLTFRQFIASLASLQGLSIDKLRSMPYWLASAVGRMLDAIWAMTRREGNPPIS 282 Query: 241 RSMIRMIGREFTVNDAAARRELGYVGRTLRDAGLQ 275 RSMIRMIGREF++NDAAARRELGYVGRT R GL+ Sbjct: 283 RSMIRMIGREFSINDAAARRELGYVGRTSRADGLR 317 >A9U7S2.1 A9U7S2_PHYPA Predicted protein (Fragment) Length = 139 Score = 254 bits (649), Expect = 8e-66, Method: Compositional matrix adjust. Identities = 123/138 (89%), Positives = 126/138 (91%) Query: 146 FAFIDHGDYPFSTCHVDNVVEAIQCALERGEGGHAFFIKDQEGQTFREFAASLANLQGLS 205 FAFID GDYPFSTCHVDNV+EAIQCALERGEGG AFFIKDQEGQTFREF ASLANLQGLS Sbjct: 1 FAFIDRGDYPFSTCHVDNVIEAIQCALERGEGGRAFFIKDQEGQTFREFVASLANLQGLS 60 Query: 206 IDKMRSMSYWLVSAIGRLFDTIWAVTRKDGDPPISRSMIRMIGREFTVNDAAARRELGYV 265 IDK+RSM YWLVSAIGRLFDTI AVTRKDGDPPISRSMIRMIGREFTVND AARRELGYV Sbjct: 61 IDKVRSMPYWLVSAIGRLFDTIGAVTRKDGDPPISRSMIRMIGREFTVNDTAARRELGYV 120 Query: 266 GRTLRDAGLQXXXXKAMR 283 GRTLRDAGLQ + R Sbjct: 121 GRTLRDAGLQSYEEPSAR 138 >Q095J3.1 Q095J3_STIAU NAD(P)-dependent steroid dehydrogenase Length = 329 Score = 196 bits (497), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 114/284 (40%), Positives = 157/284 (55%), Gaps = 8/284 (2%) Query: 2 GATPVDADLESSMPFVLPM--IDAVVHAAALFRFSGPREPFFRTNVDGTXXXXXXXXXXX 59 GA P + DL S M + V HAAA+ + PR F NV GT Sbjct: 44 GAEPCEGDLSSPEALQRGMEGCEVVFHAAAVVKMWCPRAEIFDANVRGTEHVLEAARSVG 103 Query: 60 XXTFVYISAAGIHMDNSGTLIRDADESAPTFPNH-FSAYLASKARADSLVLAANKPGFRT 118 V++S + MD GTL+ ADE+ P P+H Y ++K+ A+ LVL+ N PGF T Sbjct: 104 IQRLVHVSTEAVLMD--GTLLSRADETWP-LPSHPVGDYASTKSAAERLVLSVNSPGFTT 160 Query: 119 IALRPPAIWGPG-DPFSRALPEAVRSGQFAFIDHGDYPFSTCHVDNVVEAIQCALERGEG 177 + +RP IWG G DP A+ EAVRSG+F +ID G Y STCHV N VE + A E+G+G Sbjct: 161 VVVRPRFIWGKGKDPALAAVTEAVRSGRFWWIDGGHYQTSTCHVANCVEGMLLAAEKGKG 220 Query: 178 GHAFFIKDQEGQTFREFAASLANLQGLSIDKMRSMSYWLVSAIGRLFDTIWAVTRKDGDP 237 G A+F+ D E FREF +L QG+ +S+ WL + + + +W R G P Sbjct: 221 GQAYFLTDGEPVDFREFMTALLKAQGVEPGG-KSLPRWLGMGMATVGEALWTFLRLPGRP 279 Query: 238 PISRSMIRMIGREFTVNDAAARRELGYVGRTLRDAGLQXXXXKA 281 P +R+ + ++G+E TV+DA AR+ELGY GR R+ G Q +A Sbjct: 280 PATRAEMLVVGQEVTVSDAKARQELGYTGRMTREKGFQELAQRA 323 >Q1D036.1 Q1D036_MYXXD NAD dependent epimerase/dehydratase family protein Length = 334 Score = 166 bits (420), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 99/279 (35%), Positives = 142/279 (50%), Gaps = 10/279 (3%) Query: 2 GATPVDADLESSMPFVLPM--IDAVVHAAALFRFSGPREPFFRTNVDGTXXXXXXXXXXX 59 G P + DL M D V H AA+ + G R F+ TNV GT Sbjct: 47 GGEPFEGDLSDVEKLKAGMEGCDTVFHCAAVVKGWGARSEFYETNVRGTERVLEAARAAG 106 Query: 60 XXTFVYISAAGIHMDNSGTLIRDADESAPTFPNHFSAYLASKARADSLVLAANKPGFRTI 119 V++S + D G+ + DE+ P Y ++K A+ VL+ N P F T+ Sbjct: 107 VKRLVHVSTEAVLAD--GSPLERVDETMPLPERPIGDYPSTKGEAERRVLSVNAPDFVTV 164 Query: 120 ALRPPAIWGPGDPFSRALP---EAVRSGQFAFIDHGDYPFSTCHVDNVVEAIQCALERGE 176 +RP IWG GD + LP EAVR+G+F + G Y STCHV N VE + A E+G Sbjct: 165 VVRPRFIWGAGD--TSLLPQFQEAVRTGRFRWFGGGRYLTSTCHVANCVEGMLLAAEKGR 222 Query: 177 GGHAFFIKDQEGQTFREFAASLANLQGLSIDKMRSMSYWLVSAIGRLFDTIWAVTRKDGD 236 GG A+F+ D E FR F ++ QG+ + R++ Y + + + + D +W G Sbjct: 223 GGEAYFLTDGEPVEFRGFITAMLATQGVDAGE-RTLPYGVAATVASVGDLLWGTFGLGGR 281 Query: 237 PPISRSMIRMIGREFTVNDAAARRELGYVGRTLRDAGLQ 275 PP++R+ + ++GRE TV D ARRELGY GR R+ GL+ Sbjct: 282 PPLTRTEVLLMGREVTVRDDKARRELGYEGRRSREEGLR 320 >A7HT92.1 A7HT92_PARL1 3-beta hydroxysteroid dehydrogenase/isomerase Length = 364 Score = 158 bits (399), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 98/268 (36%), Positives = 145/268 (54%), Gaps = 6/268 (2%) Query: 1 MGATPVDADLESSMPFVLPM--IDAVVHAAALFRFSGPREPFFRTNVDGTXXXXXXXXXX 58 +GA P +ADL M +D V H AA F+ GP+ F NV G Sbjct: 52 LGAEPKEADLTDKAALARAMDGVDTVFHVAAHFKLWGPKRVFRAINVGGARNVIAAAERA 111 Query: 59 XXXTFVYISAAGIHMDNSGTLIRDADESAPTFPNHFSAYLASKARADSLVLAAN--KPGF 116 VY+SAA + M ++ A E P F+ Y A+KA A+ +VLAAN + GF Sbjct: 112 GVRRVVYVSAAAVVMGRPEPQMQ-ATEDLPLHRMRFAPYSATKAAAEKIVLAANGRREGF 170 Query: 117 RTIALRPPAIWGPGDPFSRALPEAVRSGQFAFIDHGDYPFSTCHVDNVVEAIQCALERGE 176 T+A+RPP IWGP P + E V++GQF ++ G STCHV+N+ A+ A + G+ Sbjct: 171 TTVAIRPPFIWGPDMPALDHMVETVKAGQFQWVAGGSQAMSTCHVENLCHAVILAADHGK 230 Query: 177 GGHAFFIKDQEGQTFREFAASLANLQGLSIDKMRSMSYWLVSAIGRLFDTIWAVTRKDGD 236 GG A+F+ D T + F + L +G+ R++++ L + + +W + R G+ Sbjct: 231 GGQAYFVSDDADTTLKAFLSHLLASRGVEPGD-RTVAFGLAWTMAGIMGAVWRLFRLRGE 289 Query: 237 PPISRSMIRMIGREFTVNDAAARRELGY 264 PPI+R M+R+IG++FT+N A AR ELGY Sbjct: 290 PPITRQMLRLIGKDFTLNIARARSELGY 317 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.137 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,801,742,343 Number of extensions: 67893247 Number of successful extensions: 137961 Number of sequences better than 1.0e-05: 563 Number of HSP's gapped: 137996 Number of HSP's successfully gapped: 563 Length of query: 315 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 180 Effective length of database: 1,788,859,178 Effective search space: 321994652040 Effective search space used: 321994652040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)