BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0023.p (275 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBP9.1 B3KBP9_9BACI Short-chain dehydrogenase/reductase SDR 459 e-128 Q8CXK2.1 Q8CXK2_OCEIH Oxidoreductase (Short-chain dehydrogenase:... 410 e-113 C1D3B0.1 C1D3B0_DEIDV Putative short-chain dehydrogenase 402 e-110 Q8YLW4.1 Q8YLW4_ANASP Oxidoreductase 401 e-110 B2J124.1 B2J124_NOSP7 Short-chain dehydrogenase/reductase SDR EC... 400 e-110 >B3KBP9.1 B3KBP9_9BACI Short-chain dehydrogenase/reductase SDR Length = 288 Score = 459 bits (1182), Expect = e-128, Method: Compositional matrix adjust. Identities = 215/274 (78%), Positives = 241/274 (87%) Query: 2 QDRQPGIESEMKPRPEFETSSYXXXXXXXXXXXXITGGDSGIGRAVAVHFAKEGADVVIS 61 QD +PG ESEM P+PEFE++ Y ITGGDSGIGRAVAV++AKEGADV I Sbjct: 15 QDHRPGTESEMHPKPEFESNEYKAAGKLKGKVALITGGDSGIGRAVAVYYAKEGADVSIV 74 Query: 62 YLNEHADAEETKRQVEQEGRKCILVAGDIGVEAFCQDLINKTVEGLGKLDILINNAAEQH 121 YLNEH DAEETKRQVEQEGRKC+L+AGD+G +AFC+D + +TVE LGKLDIL+NNAAEQH Sbjct: 75 YLNEHKDAEETKRQVEQEGRKCLLIAGDVGDDAFCRDAVTETVEKLGKLDILVNNAAEQH 134 Query: 122 PQDKIEDITSEQLERTFRTNIFSMFYLTKAAMPHLKKGSTIINTTSITAYRGSPQLLDYS 181 PQ KIEDIT EQLERTFRTNIF MFYLT+AAMPHL KGS+IINTTSITAYRGSPQLLDY+ Sbjct: 135 PQKKIEDITKEQLERTFRTNIFGMFYLTQAAMPHLSKGSSIINTTSITAYRGSPQLLDYA 194 Query: 182 STKGAILSFTRSLSMNLAEKGIRVNAVAPGPIWTPLIPSTFDAKKVSEFGGTQPMKRPGQ 241 STKGAI++FTRSLSMN+ +GIRVNAVAPGPIWTPLIPSTF A +V+EFG TQPMKRPGQ Sbjct: 195 STKGAIVAFTRSLSMNVVGQGIRVNAVAPGPIWTPLIPSTFPADQVAEFGATQPMKRPGQ 254 Query: 242 PEELAPAYVYLASEDSSYVSGQVMHVNGGEIVNG 275 PEELAPAYVYLAS DSSYVSGQV+HVNGGE++NG Sbjct: 255 PEELAPAYVYLASTDSSYVSGQVIHVNGGEVING 288 >Q8CXK2.1 Q8CXK2_OCEIH Oxidoreductase (Short-chain dehydrogenase:reductase family) Length = 290 Score = 410 bits (1053), Expect = e-113, Method: Compositional matrix adjust. Identities = 187/275 (68%), Positives = 229/275 (83%) Query: 1 VQDRQPGIESEMKPRPEFETSSYXXXXXXXXXXXXITGGDSGIGRAVAVHFAKEGADVVI 60 VQ +QPG+ES+M P P+F SY ITGGDSGIG++V+VH+AKEGAD+ I Sbjct: 16 VQSKQPGVESKMTPTPQFIHESYEGSNKLKGKVAVITGGDSGIGKSVSVHYAKEGADIAI 75 Query: 61 SYLNEHADAEETKRQVEQEGRKCILVAGDIGVEAFCQDLINKTVEGLGKLDILINNAAEQ 120 YL+EH DAEETKR VEQEGRKCIL+ GDIG ++FC+ + ++ GK+DIL+NNAAEQ Sbjct: 76 LYLDEHEDAEETKRLVEQEGRKCILINGDIGNQSFCEAAATEVLDNFGKVDILVNNAAEQ 135 Query: 121 HPQDKIEDITSEQLERTFRTNIFSMFYLTKAAMPHLKKGSTIINTTSITAYRGSPQLLDY 180 HPQ + DI+SEQLE+TF+TN+FSMFYLTKA +P+LKKGS IINTTS+TAY G+P L+DY Sbjct: 136 HPQQTLMDISSEQLEKTFQTNVFSMFYLTKAILPNLKKGSAIINTTSVTAYEGNPVLIDY 195 Query: 181 SSTKGAILSFTRSLSMNLAEKGIRVNAVAPGPIWTPLIPSTFDAKKVSEFGGTQPMKRPG 240 SSTKGAI+SFTR+LS +LAEKGIRVNAVAPGPIWTPLIP++F+ ++V EFGG PMKRPG Sbjct: 196 SSTKGAIVSFTRALSNDLAEKGIRVNAVAPGPIWTPLIPASFNKQQVDEFGGNTPMKRPG 255 Query: 241 QPEELAPAYVYLASEDSSYVSGQVMHVNGGEIVNG 275 QP EL PAYVYLASEDSSY+SGQ +H+NGG++VNG Sbjct: 256 QPSELGPAYVYLASEDSSYMSGQTLHINGGKVVNG 290 >C1D3B0.1 C1D3B0_DEIDV Putative short-chain dehydrogenase Length = 302 Score = 402 bits (1034), Expect = e-110, Method: Compositional matrix adjust. Identities = 191/274 (69%), Positives = 224/274 (81%) Query: 2 QDRQPGIESEMKPRPEFETSSYXXXXXXXXXXXXITGGDSGIGRAVAVHFAKEGADVVIS 61 Q QPGIESEM P Y ITGGDSGIGRAVAVHFA+EGADV I Sbjct: 29 QSVQPGIESEMSLTPVVIRDDYRGSDKLKGKVALITGGDSGIGRAVAVHFAREGADVAIL 88 Query: 62 YLNEHADAEETKRQVEQEGRKCILVAGDIGVEAFCQDLINKTVEGLGKLDILINNAAEQH 121 YLNE DA+ET V++EGR+ +L+AGDIG FC+ + +TV+ LG LDIL+NNAAEQH Sbjct: 89 YLNEEQDAQETLAMVQEEGRRGVLIAGDIGDVEFCKQAVQQTVKELGGLDILVNNAAEQH 148 Query: 122 PQDKIEDITSEQLERTFRTNIFSMFYLTKAAMPHLKKGSTIINTTSITAYRGSPQLLDYS 181 PQD I DIT EQLERTFRTNIF+MFYLT+AAMPHLK GSTIINTTS+TAY+GSPQLLDYS Sbjct: 149 PQDSISDITPEQLERTFRTNIFAMFYLTQAAMPHLKPGSTIINTTSVTAYKGSPQLLDYS 208 Query: 182 STKGAILSFTRSLSMNLAEKGIRVNAVAPGPIWTPLIPSTFDAKKVSEFGGTQPMKRPGQ 241 STKGAI++FTRSLS NLAE+GIRVNAVAPGPIWTPLIP+TFDA++V+ G + P+KRPGQ Sbjct: 209 STKGAIVAFTRSLSQNLAEQGIRVNAVAPGPIWTPLIPATFDAERVAAHGQSTPLKRPGQ 268 Query: 242 PEELAPAYVYLASEDSSYVSGQVMHVNGGEIVNG 275 P E+AP++V+LAS+DSSY+SGQV+H NGGE+VNG Sbjct: 269 PAEVAPSFVFLASDDSSYISGQVLHPNGGEVVNG 302 >Q8YLW4.1 Q8YLW4_ANASP Oxidoreductase Length = 285 Score = 401 bits (1031), Expect = e-110, Method: Compositional matrix adjust. Identities = 191/269 (71%), Positives = 222/269 (82%) Query: 7 GIESEMKPRPEFETSSYXXXXXXXXXXXXITGGDSGIGRAVAVHFAKEGADVVISYLNEH 66 G ES+M+P+P+ + + Y ITGGDSGIGRAVA+ +AKEGADV YL+EH Sbjct: 17 GTESKMQPKPQADDAQYRGSGKLKDKVALITGGDSGIGRAVAIAYAKEGADVAFVYLSEH 76 Query: 67 ADAEETKRQVEQEGRKCILVAGDIGVEAFCQDLINKTVEGLGKLDILINNAAEQHPQDKI 126 DAEETK VE++GR+ + +AGDI EAFCQ I +TV+ GKLDILINNAAEQHPQ+ I Sbjct: 77 DDAEETKNLVEEQGRRAVSIAGDITDEAFCQRAIQQTVDEFGKLDILINNAAEQHPQESI 136 Query: 127 EDITSEQLERTFRTNIFSMFYLTKAAMPHLKKGSTIINTTSITAYRGSPQLLDYSSTKGA 186 EDIT EQLERTFRTNIFSMFYLTKAA+ HLKKGS IINTTS+TAY+GSPQLLDYSSTKGA Sbjct: 137 EDITKEQLERTFRTNIFSMFYLTKAAIKHLKKGSAIINTTSVTAYKGSPQLLDYSSTKGA 196 Query: 187 ILSFTRSLSMNLAEKGIRVNAVAPGPIWTPLIPSTFDAKKVSEFGGTQPMKRPGQPEELA 246 I++FTRSLS NL KGIRVNAVAPGPIWTPLIPSTF A+KV FG PM+R GQPEE+A Sbjct: 197 IVAFTRSLSQNLISKGIRVNAVAPGPIWTPLIPSTFPAEKVETFGKQVPMQRAGQPEEVA 256 Query: 247 PAYVYLASEDSSYVSGQVMHVNGGEIVNG 275 P+YV+LAS+DSSY+SGQV+H NGGE+VNG Sbjct: 257 PSYVFLASDDSSYMSGQVLHPNGGEVVNG 285 >B2J124.1 B2J124_NOSP7 Short-chain dehydrogenase/reductase SDR EC=1.1.1.100 Length = 312 Score = 400 bits (1028), Expect = e-110, Method: Compositional matrix adjust. Identities = 189/274 (68%), Positives = 221/274 (80%) Query: 2 QDRQPGIESEMKPRPEFETSSYXXXXXXXXXXXXITGGDSGIGRAVAVHFAKEGADVVIS 61 Q PG+ESEM P+P+ + + Y ITG DSGIGRAVA+ FAKEGADV I Sbjct: 39 QQEVPGVESEMTPKPKADDAQYRGSGKLQDKVALITGADSGIGRAVAIAFAKEGADVAIL 98 Query: 62 YLNEHADAEETKRQVEQEGRKCILVAGDIGVEAFCQDLINKTVEGLGKLDILINNAAEQH 121 YLNEH DA+ETK VE++GR+ + +AGDIG E FCQ I +TV GKLDILINNAAEQH Sbjct: 99 YLNEHDDAKETKHLVEKQGRRAVTIAGDIGDETFCQQAIQQTVGEFGKLDILINNAAEQH 158 Query: 122 PQDKIEDITSEQLERTFRTNIFSMFYLTKAAMPHLKKGSTIINTTSITAYRGSPQLLDYS 181 P+ IE+IT EQLERTFRTNIFSMF++TKAA+ HL+ GS IINTTS+TAY+GSPQLLDYS Sbjct: 159 PKQSIEEITKEQLERTFRTNIFSMFFMTKAALKHLQTGSAIINTTSVTAYKGSPQLLDYS 218 Query: 182 STKGAILSFTRSLSMNLAEKGIRVNAVAPGPIWTPLIPSTFDAKKVSEFGGTQPMKRPGQ 241 STKGAI++FTRSLS NL KGIRVNAVAPGPIWTPLIPSTF +KV FG PM+R GQ Sbjct: 219 STKGAIVAFTRSLSQNLVSKGIRVNAVAPGPIWTPLIPSTFPEEKVESFGKQVPMQRAGQ 278 Query: 242 PEELAPAYVYLASEDSSYVSGQVMHVNGGEIVNG 275 PEE+AP+YV+LAS+D+SYVSGQV+HVNGGE+VNG Sbjct: 279 PEEVAPSYVFLASDDASYVSGQVLHVNGGEVVNG 312 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.132 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,537,891,323 Number of extensions: 61261156 Number of successful extensions: 234877 Number of sequences better than 1.0e-05: 30527 Number of HSP's gapped: 181610 Number of HSP's successfully gapped: 30615 Length of query: 275 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 141 Effective length of database: 1,798,280,193 Effective search space: 253557507213 Effective search space used: 253557507213 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)