BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0022.p (200 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A7GLF5.1 A7GLF5_BACCN Thiamine monophosphate synthase 115 3e-24 Q2B0F4.1 Q2B0F4_9BACI Transcriptional regulator TenI 108 3e-22 A4IKR3.1 A4IKR3_GEOTN Regulatory protein TenI 107 7e-22 B4BRU7.1 B4BRU7_9BACI Thiamine monophosphate synthase 107 7e-22 B7YEN4.1 B7YEN4_9BACI Thiamine monophosphate synthase 104 4e-21 >A7GLF5.1 A7GLF5_BACCN Thiamine monophosphate synthase Length = 206 Score = 115 bits (287), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 61/184 (33%), Positives = 91/184 (49%) Query: 3 EIHLISDGKLEPGRFINLAAAVYPLVDYIHLREKHRPALELLDMAKQLLLAGVPASSLVI 62 E+H+IS+G++ N+A + +DY+H+RE+ + EL + + LL G PAS LV+ Sbjct: 4 ELHVISNGQMTFEELTNVAMQMESEIDYLHIREREKSTKELYEGVEGLLKRGFPASKLVL 63 Query: 63 NDRLDXXXXXXXXXXXXXWNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDYGLFG 122 NDR+D + D ++G Sbjct: 64 NDRIDIAILLNIPRVQLGYRSVDVKSVKEKFSYLHVGYSVHSLEEAIVAFKNGADSLVYG 123 Query: 123 HVFPTACKPGQQERGLGLLAEAVRRSRMPLIAIGGIEPGNAAQVIRQGAAGIAVMSGICS 182 HVFPT+CK G RGL +++ RR +P+ AIGGI P N +V++ G +GIAVMSGI S Sbjct: 124 HVFPTSCKKGVPARGLEEISDMARRLTIPITAIGGITPENTTEVLKSGVSGIAVMSGIVS 183 Query: 183 TEDP 186 +P Sbjct: 184 DRNP 187 >Q2B0F4.1 Q2B0F4_9BACI Transcriptional regulator TenI Length = 221 Score = 108 bits (270), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 63/184 (34%), Positives = 90/184 (48%), Gaps = 1/184 (0%) Query: 3 EIHLISDGKLEPGRFINLAAAVYPLVDYIHLREKHRPALELLDMAKQLLLAGVPASSLVI 62 ++H IS G+ FI +A+++ VDYIH+RE H A ++ +M + LL GVP ++I Sbjct: 17 QLHAISAGQ-PAEEFIRIASSISRNVDYIHIREHHLIAADIYEMGRILLEKGVPGEQIII 75 Query: 63 NDRLDXXXXXXXXXXXXXWNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDYGLFG 122 N+R+D ++ DY ++G Sbjct: 76 NNRVDAAAALGVKGVQLGYHSLPVREVRITFPGLLIGKSVHSIEEAVQAEEDGADYVMYG 135 Query: 123 HVFPTACKPGQQERGLGLLAEAVRRSRMPLIAIGGIEPGNAAQVIRQGAAGIAVMSGICS 182 H FPT+ KPG + RGL L E R +P+IA+GGI P N QVIR GA+G AV+SGI Sbjct: 136 HCFPTSSKPGMEPRGLSSLEEMTGRLSIPVIALGGISPDNMEQVIRAGASGAAVLSGIFK 195 Query: 183 TEDP 186 P Sbjct: 196 AVSP 199 >A4IKR3.1 A4IKR3_GEOTN Regulatory protein TenI Length = 203 Score = 107 bits (267), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 60/184 (32%), Positives = 84/184 (45%) Query: 4 IHLISDGKLEPGRFINLAAAVYPLVDYIHLREKHRPALELLDMAKQLLLAGVPASSLVIN 63 +H +S G+ F + ++P D IH+REK + A E+ LL GVP +++N Sbjct: 4 LHFVSTGRQTADEFAAICQHIHPYADCIHIREKEKTAREVAAFVTALLRVGVPPQKIIVN 63 Query: 64 DRLDXXXXXXXXXXXXXWNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDYGLFGH 123 DR+D ++ + LFGH Sbjct: 64 DRVDVAAVYGVHGVQLAYHSLPVRAVRRSFPSLTVGCSVHGLAEAKQAEEDGAHFCLFGH 123 Query: 124 VFPTACKPGQQERGLGLLAEAVRRSRMPLIAIGGIEPGNAAQVIRQGAAGIAVMSGICST 183 +FPTA KPG RG+ LL E +P+IAIGGI GNA +V+ GAAG+AV+S I Sbjct: 124 IFPTASKPGVLPRGVDLLKEIAAAVHIPVIAIGGIHAGNARRVLEAGAAGVAVLSAIFFA 183 Query: 184 EDPV 187 DPV Sbjct: 184 ADPV 187 >B4BRU7.1 B4BRU7_9BACI Thiamine monophosphate synthase Length = 203 Score = 107 bits (267), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 60/184 (32%), Positives = 84/184 (45%) Query: 4 IHLISDGKLEPGRFINLAAAVYPLVDYIHLREKHRPALELLDMAKQLLLAGVPASSLVIN 63 +H +S G+ F + ++P D IH+REK + A E+ LL GVP +++N Sbjct: 4 LHFVSTGRQTADEFAAICQHIHPYADCIHIREKEKTAREVAAFVTALLRVGVPPQKIIVN 63 Query: 64 DRLDXXXXXXXXXXXXXWNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDYGLFGH 123 DR+D ++ + LFGH Sbjct: 64 DRVDVAAVYGVHGVQLAYHSLPVRAVRRSFPSLTVGCSVHGLAEAKQAEEDGAHFCLFGH 123 Query: 124 VFPTACKPGQQERGLGLLAEAVRRSRMPLIAIGGIEPGNAAQVIRQGAAGIAVMSGICST 183 +FPTA KPG RG+ LL E +P+IAIGGI GNA +V+ GAAG+AV+S I Sbjct: 124 IFPTASKPGVLPRGVDLLKEIAAAVHIPVIAIGGIHAGNARRVLEAGAAGVAVLSAIFFA 183 Query: 184 EDPV 187 DPV Sbjct: 184 ADPV 187 >B7YEN4.1 B7YEN4_9BACI Thiamine monophosphate synthase Length = 201 Score = 104 bits (260), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 60/184 (32%), Positives = 84/184 (45%) Query: 4 IHLISDGKLEPGRFINLAAAVYPLVDYIHLREKHRPALELLDMAKQLLLAGVPASSLVIN 63 +H +S G+ F + A +P D IH+REK + A E+ LL GVP +++N Sbjct: 4 LHFVSTGRQTVDEFAAICAHTHPYADLIHIREKGKTAREVAAFVAALLRVGVPPQKIIVN 63 Query: 64 DRLDXXXXXXXXXXXXXWNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDYGLFGH 123 DR+D ++ + L+GH Sbjct: 64 DRVDVAAVYGVKGVQLAYHSLPVRAVRRSFPDLTVGCSVHGSEEAKQAEQDGAHFCLYGH 123 Query: 124 VFPTACKPGQQERGLGLLAEAVRRSRMPLIAIGGIEPGNAAQVIRQGAAGIAVMSGICST 183 +FPT KPG RGL LAE V +P+IAIGGI GNA +V+ GAAG+AV+S + Sbjct: 124 IFPTDSKPGLPPRGLDSLAEIVAAVSIPVIAIGGIHAGNARRVLEAGAAGVAVLSAVFFA 183 Query: 184 EDPV 187 DPV Sbjct: 184 ADPV 187 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.141 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 792,112,007 Number of extensions: 23835664 Number of successful extensions: 71137 Number of sequences better than 1.0e-05: 132 Number of HSP's gapped: 71501 Number of HSP's successfully gapped: 175 Length of query: 200 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 71 Effective length of database: 1,845,385,268 Effective search space: 131022354028 Effective search space used: 131022354028 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)