BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0020.p (249 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A1HPQ6.1 A1HPQ6_9FIRM Thiazole biosynthesis family protein 319 1e-85 A5D4K1.1 THIG_PELTS Thiazole biosynthesis protein thiG 306 8e-82 B4BDB6.1 B4BDB6_CLOTM Thiazole biosynthesis family protein 306 9e-82 A3DD04.1 THIG_CLOTH Thiazole biosynthesis protein thiG 306 9e-82 Q2RHX4.1 THIG_MOOTA Thiazole biosynthesis protein thiG 306 1e-81 >A1HPQ6.1 A1HPQ6_9FIRM Thiazole biosynthesis family protein Length = 256 Score = 319 bits (818), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 164/249 (65%), Positives = 194/249 (77%), Gaps = 1/249 (0%) Query: 2 IGGITLSSRLFIGTGKYSRNTLIPEVVASSGSQVITVALRRVDPESRD-NIVSHIPPHMT 60 IG L+SRLF+GTGK++ N LIPE VA+SG+QV+TVALRRVD +S D NI+++IP + Sbjct: 7 IGDKELTSRLFLGTGKFASNKLIPEAVAASGAQVVTVALRRVDLDSSDENILNYIPKNCI 66 Query: 61 LLPNTSGARTAGEAVRIXXXXXXXXXXNWVKIEVINDQKYLLPDNLETIRATEILAAEGF 120 L+PNTSGAR A EAVRI NWVKIEVI+D +YLLPDN ETI+ATEILAAEGF Sbjct: 67 LMPNTSGARNAEEAVRIARLARAAGCGNWVKIEVISDNRYLLPDNYETIKATEILAAEGF 126 Query: 121 VVLPYMSPDLSAAIRMKEAGAAAIMPLGSPIGSNRGLRTKELLRILISEVDLPVIVDAGI 180 VVLPYMSPDL AA R+ EAGAAA+MPLG+PIGSNRGL+TKEL+RILI E+ LP+IVDAGI Sbjct: 127 VVLPYMSPDLMAAKRLAEAGAAAVMPLGAPIGSNRGLKTKELIRILIEEIPLPIIVDAGI 186 Query: 181 GRPSXXXXXXXXXXXXXXXNTAIATARDPLLMAEAFREAVSSGRKAYLAGLGPVEENAVA 240 GRPS NTAIATA DP+ MA+AF AV++GR AYLAG G V++ A A Sbjct: 187 GRPSEAAEAMELGAAAVLVNTAIATAADPVAMAKAFGLAVAAGRTAYLAGPGAVQQYASA 246 Query: 241 SSPLTGFLK 249 SSPLTGFL+ Sbjct: 247 SSPLTGFLR 255 >A5D4K1.1 THIG_PELTS Thiazole biosynthesis protein thiG Length = 256 Score = 306 bits (785), Expect = 8e-82, Method: Compositional matrix adjust. Identities = 160/249 (64%), Positives = 186/249 (74%), Gaps = 1/249 (0%) Query: 2 IGGITLSSRLFIGTGKYSRNTLIPEVVASSGSQVITVALRRVDPE-SRDNIVSHIPPHMT 60 IGG L+SRLF+GTGK+S N LIPE V +SG+QV+TVALRR+D E +NI +++P Sbjct: 7 IGGRELTSRLFLGTGKFSSNRLIPEAVRASGAQVVTVALRRIDLEYEEENIAAYVPKDCI 66 Query: 61 LLPNTSGARTAGEAVRIXXXXXXXXXXNWVKIEVINDQKYLLPDNLETIRATEILAAEGF 120 L+PNTSGAR A EAVRI NWVKIEVI D +YLLPDN ETI+ATEILAAEGF Sbjct: 67 LMPNTSGARNAQEAVRIARLARAAGCGNWVKIEVITDNRYLLPDNYETIKATEILAAEGF 126 Query: 121 VVLPYMSPDLSAAIRMKEAGAAAIMPLGSPIGSNRGLRTKELLRILISEVDLPVIVDAGI 180 VVLPYMSPDL A ++ EAGAAA+MPLG+PIGSNRGLRTKEL+RILI E+ LP+IVDAG+ Sbjct: 127 VVLPYMSPDLMVAKKLAEAGAAAVMPLGAPIGSNRGLRTKELVRILIDEIPLPIIVDAGL 186 Query: 181 GRPSXXXXXXXXXXXXXXXNTAIATARDPLLMAEAFREAVSSGRKAYLAGLGPVEENAVA 240 GRPS NTAIATA DP+ MA AF AV +GR AYLAG G +E A A Sbjct: 187 GRPSEAAEAMEMGAAAVLVNTAIATAGDPVAMARAFGLAVKAGRLAYLAGPGETQEYARA 246 Query: 241 SSPLTGFLK 249 SSPLTGFL+ Sbjct: 247 SSPLTGFLR 255 >B4BDB6.1 B4BDB6_CLOTM Thiazole biosynthesis family protein Length = 255 Score = 306 bits (785), Expect = 9e-82, Method: Compositional matrix adjust. Identities = 153/250 (61%), Positives = 194/250 (77%), Gaps = 1/250 (0%) Query: 1 VIGGITLSSRLFIGTGKYSRNTLIPEVVASSGSQVITVALRRVDPES-RDNIVSHIPPHM 59 +IGG + SRLFIGTGK+ N +IP+VV SG+QV+TVALRR+D +S +N++ +IP Sbjct: 6 IIGGREIISRLFIGTGKFPANRIIPDVVRMSGAQVVTVALRRIDFDSDEENMLRYIPKDC 65 Query: 60 TLLPNTSGARTAGEAVRIXXXXXXXXXXNWVKIEVINDQKYLLPDNLETIRATEILAAEG 119 L+PNTSGAR A EAVRI N++KIEVI+D +YLLPDNLETI+ATEIL EG Sbjct: 66 ILMPNTSGARNAEEAVRIARLARASGCGNFIKIEVISDNRYLLPDNLETIKATEILVKEG 125 Query: 120 FVVLPYMSPDLSAAIRMKEAGAAAIMPLGSPIGSNRGLRTKELLRILISEVDLPVIVDAG 179 F+VLPYM+PDL A R+K+AGAAA+MPLG+PIG+N+GL+T+E+++ILI E+D+PVIVDAG Sbjct: 126 FIVLPYMNPDLMDARRLKDAGAAAVMPLGAPIGTNKGLKTREMIKILIDEIDIPVIVDAG 185 Query: 180 IGRPSXXXXXXXXXXXXXXXNTAIATARDPLLMAEAFREAVSSGRKAYLAGLGPVEENAV 239 IG+PS NTAIATA DP++MAEAF AV +GRKAYL+GLGPV+E A Sbjct: 186 IGKPSDACEAMELGADAVLVNTAIATAGDPVVMAEAFANAVIAGRKAYLSGLGPVKEYAE 245 Query: 240 ASSPLTGFLK 249 ASSPLTGFL+ Sbjct: 246 ASSPLTGFLR 255 >A3DD04.1 THIG_CLOTH Thiazole biosynthesis protein thiG Length = 255 Score = 306 bits (785), Expect = 9e-82, Method: Compositional matrix adjust. Identities = 153/250 (61%), Positives = 194/250 (77%), Gaps = 1/250 (0%) Query: 1 VIGGITLSSRLFIGTGKYSRNTLIPEVVASSGSQVITVALRRVDPES-RDNIVSHIPPHM 59 +IGG + SRLFIGTGK+ N +IP+VV SG+QV+TVALRR+D +S +N++ +IP Sbjct: 6 IIGGREIISRLFIGTGKFPANRIIPDVVRMSGAQVVTVALRRIDFDSDEENMLRYIPKDC 65 Query: 60 TLLPNTSGARTAGEAVRIXXXXXXXXXXNWVKIEVINDQKYLLPDNLETIRATEILAAEG 119 L+PNTSGAR A EAVRI N++KIEVI+D +YLLPDNLETI+ATEIL EG Sbjct: 66 ILMPNTSGARNAEEAVRIARLARASGCGNFIKIEVISDNRYLLPDNLETIKATEILVKEG 125 Query: 120 FVVLPYMSPDLSAAIRMKEAGAAAIMPLGSPIGSNRGLRTKELLRILISEVDLPVIVDAG 179 F+VLPYM+PDL A R+K+AGAAA+MPLG+PIG+N+GL+T+E+++ILI E+D+PVIVDAG Sbjct: 126 FIVLPYMNPDLMDARRLKDAGAAAVMPLGAPIGTNKGLKTREMIKILIDEIDIPVIVDAG 185 Query: 180 IGRPSXXXXXXXXXXXXXXXNTAIATARDPLLMAEAFREAVSSGRKAYLAGLGPVEENAV 239 IG+PS NTAIATA DP++MAEAF AV +GRKAYL+GLGPV+E A Sbjct: 186 IGKPSDACEAMELGADAVLVNTAIATAGDPVVMAEAFANAVIAGRKAYLSGLGPVKEYAE 245 Query: 240 ASSPLTGFLK 249 ASSPLTGFL+ Sbjct: 246 ASSPLTGFLR 255 >Q2RHX4.1 THIG_MOOTA Thiazole biosynthesis protein thiG Length = 255 Score = 306 bits (783), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 153/249 (61%), Positives = 188/249 (75%), Gaps = 1/249 (0%) Query: 1 VIGGITLSSRLFIGTGKYSRNTLIPEVVASSGSQVITVALRRVDPES-RDNIVSHIPPHM 59 +IG L+SRLFIGTGK++ + ++ + V+ SG+QV+TVALRRVD ++ +++ + IP Sbjct: 6 IIGDKALTSRLFIGTGKFASHEIMGQAVSQSGAQVVTVALRRVDLDNPQESELKFIPAGC 65 Query: 60 TLLPNTSGARTAGEAVRIXXXXXXXXXXNWVKIEVINDQKYLLPDNLETIRATEILAAEG 119 L+PNTSGARTA EAV+I NWVKIEV+ DQ+YLLPDN ET++ATEILA EG Sbjct: 66 VLMPNTSGARTAEEAVKIARIARAAGCGNWVKIEVVTDQRYLLPDNYETVKATEILAKEG 125 Query: 120 FVVLPYMSPDLSAAIRMKEAGAAAIMPLGSPIGSNRGLRTKELLRILISEVDLPVIVDAG 179 FVVLPYM+PDL A R+KEAGAAAIMPLG+PIGSNRGL+T+E++RILI E+DLP+IVDAG Sbjct: 126 FVVLPYMNPDLMVAKRLKEAGAAAIMPLGAPIGSNRGLKTREMIRILIEEIDLPIIVDAG 185 Query: 180 IGRPSXXXXXXXXXXXXXXXNTAIATARDPLLMAEAFREAVSSGRKAYLAGLGPVEENAV 239 IG+PS NTAIATARDP+ MA AF AV +GR AYLAGL P E A Sbjct: 186 IGKPSEAMEAMEMGAAAVLVNTAIATARDPVAMARAFSMAVEAGRMAYLAGLAPTREYAE 245 Query: 240 ASSPLTGFL 248 ASSPLTGFL Sbjct: 246 ASSPLTGFL 254 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,213,567,310 Number of extensions: 41663159 Number of successful extensions: 105310 Number of sequences better than 1.0e-05: 1033 Number of HSP's gapped: 103687 Number of HSP's successfully gapped: 1033 Length of query: 249 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 117 Effective length of database: 1,817,122,223 Effective search space: 212603300091 Effective search space used: 212603300091 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)