BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0019.p (370 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A5D4K2.1 A5D4K2_PELTS Thiamine biosynthesis enzyme ThiH and rela... 439 e-121 C1TJF9.1 C1TJF9_9FIRM Tyrosine lyase ThiH 435 e-120 A3DD05.1 A3DD05_CLOTH Thiazole biosynthesis protein ThiH 422 e-116 B4BDB7.1 B4BDB7_CLOTM Thiazole biosynthesis protein ThiH 422 e-116 A1HPQ5.1 A1HPQ5_9FIRM Thiazole biosynthesis protein ThiH 421 e-116 >A5D4K2.1 A5D4K2_PELTS Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes Length = 366 Score = 439 bits (1128), Expect = e-121, Method: Compositional matrix adjust. Identities = 211/366 (57%), Positives = 262/366 (71%) Query: 1 MSFYESLTRLEQLPYESLWQQFQVEDVKRALCKDRLDESDLLALLSPAAEPCLEEIARRA 60 MSFYE ++ + V+ VKRAL K RL E D LALLSPAAE CLEE+AR+A Sbjct: 1 MSFYEVRKEYAGFDFKGFFAGVTVDRVKRALSKSRLTEWDYLALLSPAAEECLEEMARKA 60 Query: 61 QRLTRTHFGHVMQLFTPMYLADFCVNHCTYCSFSSIYDFPRKRLTPEEVRQEAIAIASTG 120 RLT HFG V+ L+TP+YLA++CVN C YC F + RK+LTP EV +EA IA+TG Sbjct: 61 HRLTVQHFGRVILLYTPLYLANYCVNRCVYCGFKANNRLERKKLTPAEVEREAGIIAATG 120 Query: 121 LRHLLVLTGESRSASPASYVKECVEILHEYFPSVSIEVNPLSTAEYRELREAGVDGLTLY 180 L+H+L+LTGESR SP SY++ECV+IL +YF S+SIE+ PL +EY EL AGVDGLT+Y Sbjct: 121 LKHILILTGESRRHSPVSYIRECVKILKKYFTSISIEIYPLEESEYAELIAAGVDGLTIY 180 Query: 181 QEVYHQETYRKLHVKGPKRVFRNRLDAPERGCQAGFRAVNIGALLGMYDWRQEAFMTAMH 240 QEVY +E Y +LH GPKR +R RL+APER C+AG R VN+GALLG++DWR EAF T +H Sbjct: 181 QEVYDEEVYAELHPAGPKRNYRYRLEAPERACRAGMRTVNVGALLGLHDWRTEAFFTGLH 240 Query: 241 ARYLQDKYPEXXXXLSAPRFRPYLGEFNPDSDVTDRALVQIILAYRLFLPRSGITLSTRE 300 A YLQD +P+ +S PR RP LG F P V D+ LVQ +LA+RL++PR GIT+STRE Sbjct: 241 ASYLQDTFPDVEVSISPPRMRPQLGGFQPRVKVNDKDLVQYMLAFRLYMPRGGITISTRE 300 Query: 301 PAPLRNHLIHLGITKMSAGVSTEVGGHTQDGGTPQFEISDERSVEEITDMLQANGLQPVF 360 A LR+HL+ LG TKMSAG T VGG + T QFEISD RSV E+ ML G QPV+ Sbjct: 301 RAELRDHLVRLGATKMSAGSCTAVGGRSGHESTGQFEISDGRSVAEMARMLYDQGYQPVY 360 Query: 361 KDWDLL 366 KDW ++ Sbjct: 361 KDWQMI 366 >C1TJF9.1 C1TJF9_9FIRM Tyrosine lyase ThiH Length = 372 Score = 435 bits (1118), Expect = e-120, Method: Compositional matrix adjust. Identities = 208/363 (57%), Positives = 263/363 (72%) Query: 1 MSFYESLTRLEQLPYESLWQQFQVEDVKRALCKDRLDESDLLALLSPAAEPCLEEIARRA 60 +SFY L R E +E ++Q +KR + + RL+E D LALLSP AE LEE+A++A Sbjct: 4 VSFYGELQRYENFDFELFFKQVTDAGIKRIIAQHRLNERDYLALLSPRAENFLEEMAQKA 63 Query: 61 QRLTRTHFGHVMQLFTPMYLADFCVNHCTYCSFSSIYDFPRKRLTPEEVRQEAIAIASTG 120 RLT HFG V+ LFTPMYLA++CVN C YC F RK+L+P EV +EA IA+TG Sbjct: 64 HRLTVQHFGRVIFLFTPMYLANYCVNQCVYCGFQVHNRLERKKLSPAEVEKEAKIIAATG 123 Query: 121 LRHLLVLTGESRSASPASYVKECVEILHEYFPSVSIEVNPLSTAEYRELREAGVDGLTLY 180 L+H+L+LTGESR SP SY+++CVE+L +YF SVSIE+ PL EY EL AGVDGLT+Y Sbjct: 124 LKHILILTGESRQESPVSYIRDCVEVLKKYFTSVSIEIYPLEEDEYAELIAAGVDGLTMY 183 Query: 181 QEVYHQETYRKLHVKGPKRVFRNRLDAPERGCQAGFRAVNIGALLGMYDWRQEAFMTAMH 240 QEVY++E Y +LH GPKR +R RLDAPER C+AG R VN+GALLG++DWR EAF T +H Sbjct: 184 QEVYNEEVYAELHPGGPKRNYRFRLDAPERACRAGVRTVNVGALLGLHDWRSEAFFTGLH 243 Query: 241 ARYLQDKYPEXXXXLSAPRFRPYLGEFNPDSDVTDRALVQIILAYRLFLPRSGITLSTRE 300 A YLQ + + +S PR RP+LG F P +V+D+ LVQ +LA+RLF+PR GIT+STRE Sbjct: 244 ADYLQKNFTDVEVSISPPRMRPHLGGFQPRVEVSDQNLVQYLLAFRLFMPRGGITVSTRE 303 Query: 301 PAPLRNHLIHLGITKMSAGVSTEVGGHTQDGGTPQFEISDERSVEEITDMLQANGLQPVF 360 A LR+HL+ LG TKMSAG T VGG + T QFEISDER+V E+ DML + G QPV+ Sbjct: 304 RAELRDHLVRLGATKMSAGSCTAVGGRSDQESTGQFEISDERNVVEMADMLYSVGYQPVY 363 Query: 361 KDW 363 KDW Sbjct: 364 KDW 366 >A3DD05.1 A3DD05_CLOTH Thiazole biosynthesis protein ThiH Length = 369 Score = 422 bits (1086), Expect = e-116, Method: Compositional matrix adjust. Identities = 206/366 (56%), Positives = 261/366 (71%), Gaps = 3/366 (0%) Query: 1 MSFYESLTRLEQLPYESLWQQFQVEDVKRALCKDR-LDESDLLALLSPAAEPCLEEIARR 59 MSFYE + +E+ + Q D+ + KDR L E + L LLS A LE +A++ Sbjct: 1 MSFYERYLEYKNFDFENFFDQVTDRDIINIINKDRRLSELEFLMLLSKKAVKYLEPLAQK 60 Query: 60 AQRLTRTHFGHVMQLFTPMYLADFCVNHCTYCSFSSIYDFPRKRLTPEEVRQEAIAIAST 119 A R+T +FG V+ L+TPMYLA++CVN C YC F+ + R++LT +EV +EA AI+ST Sbjct: 61 ANRITVQNFGKVIFLYTPMYLANYCVNQCIYCGFNITNNIKRRKLTLDEVEKEAYAISST 120 Query: 120 GLRHLLVLTGESRSASPASYVKECVEILHEYFPSVSIEVNPLSTAEYRELREAGVDGLTL 179 GLRH+L+LTGESR SP Y+K+CV+IL +YF S+SIEV PL EY EL EAGVDGLT+ Sbjct: 121 GLRHILILTGESRKESPVQYIKDCVKILQKYFRSISIEVYPLEENEYAELIEAGVDGLTI 180 Query: 180 YQEVYHQETYRKLHVKGPKRVFRNRLDAPERGCQAGFRAVNIGALLGMYDWRQEAFMTAM 239 YQEVY +E Y+ LH+KGPKR + RLDAPER C+A R VNIGALLG++DWR EAF T + Sbjct: 181 YQEVYDEEKYKALHLKGPKRNYLYRLDAPERACKASMRNVNIGALLGLHDWRTEAFYTGL 240 Query: 240 HARYLQDKYPEXXXXLSAPRFRPY-LGEFNPDSDVTDRALVQIILAYRLFLPRSGITLST 298 HA YLQ+KYP+ LS PR RP+ G F PD V DR LVQI++AYRLF+PR+GI +ST Sbjct: 241 HADYLQNKYPDVEIGLSLPRIRPHPCGSFVPDCKVEDRDLVQIMIAYRLFMPRAGIAIST 300 Query: 299 REPAPLRNHLIHLGITKMSAGVSTEVGGHT-QDGGTPQFEISDERSVEEITDMLQANGLQ 357 RE LRN+LI LG+TKMSA STEVGGHT D QF+++D R VEE+ M+ + G Q Sbjct: 301 RERESLRNNLIGLGVTKMSAASSTEVGGHTLGDKSDGQFDVNDRRGVEEMRQMIYSKGYQ 360 Query: 358 PVFKDW 363 PVFKDW Sbjct: 361 PVFKDW 366 >B4BDB7.1 B4BDB7_CLOTM Thiazole biosynthesis protein ThiH Length = 369 Score = 422 bits (1086), Expect = e-116, Method: Compositional matrix adjust. Identities = 206/366 (56%), Positives = 261/366 (71%), Gaps = 3/366 (0%) Query: 1 MSFYESLTRLEQLPYESLWQQFQVEDVKRALCKDR-LDESDLLALLSPAAEPCLEEIARR 59 MSFYE + +E+ + Q D+ + KDR L E + L LLS A LE +A++ Sbjct: 1 MSFYERYLEYKNFDFENFFDQVTDRDIINIINKDRRLSELEFLMLLSKKAVKYLEPLAQK 60 Query: 60 AQRLTRTHFGHVMQLFTPMYLADFCVNHCTYCSFSSIYDFPRKRLTPEEVRQEAIAIAST 119 A R+T +FG V+ L+TPMYLA++CVN C YC F+ + R++LT +EV +EA AI+ST Sbjct: 61 ANRITVQNFGKVIFLYTPMYLANYCVNQCIYCGFNITNNIKRRKLTLDEVEKEAYAISST 120 Query: 120 GLRHLLVLTGESRSASPASYVKECVEILHEYFPSVSIEVNPLSTAEYRELREAGVDGLTL 179 GLRH+L+LTGESR SP Y+K+CV+IL +YF S+SIEV PL EY EL EAGVDGLT+ Sbjct: 121 GLRHILILTGESRKESPVQYIKDCVKILQKYFRSISIEVYPLEENEYAELIEAGVDGLTI 180 Query: 180 YQEVYHQETYRKLHVKGPKRVFRNRLDAPERGCQAGFRAVNIGALLGMYDWRQEAFMTAM 239 YQEVY +E Y+ LH+KGPKR + RLDAPER C+A R VNIGALLG++DWR EAF T + Sbjct: 181 YQEVYDEEKYKALHLKGPKRNYLYRLDAPERACKASMRNVNIGALLGLHDWRTEAFYTGL 240 Query: 240 HARYLQDKYPEXXXXLSAPRFRPY-LGEFNPDSDVTDRALVQIILAYRLFLPRSGITLST 298 HA YLQ+KYP+ LS PR RP+ G F PD V DR LVQI++AYRLF+PR+GI +ST Sbjct: 241 HADYLQNKYPDVEIGLSLPRIRPHPCGSFVPDCKVEDRDLVQIMIAYRLFMPRAGIAIST 300 Query: 299 REPAPLRNHLIHLGITKMSAGVSTEVGGHT-QDGGTPQFEISDERSVEEITDMLQANGLQ 357 RE LRN+LI LG+TKMSA STEVGGHT D QF+++D R VEE+ M+ + G Q Sbjct: 301 RERESLRNNLIGLGVTKMSAASSTEVGGHTLGDKSDGQFDVNDRRGVEEMRQMIYSKGYQ 360 Query: 358 PVFKDW 363 PVFKDW Sbjct: 361 PVFKDW 366 >A1HPQ5.1 A1HPQ5_9FIRM Thiazole biosynthesis protein ThiH Length = 367 Score = 421 bits (1083), Expect = e-116, Method: Compositional matrix adjust. Identities = 202/365 (55%), Positives = 257/365 (70%) Query: 2 SFYESLTRLEQLPYESLWQQFQVEDVKRALCKDRLDESDLLALLSPAAEPCLEEIARRAQ 61 +FY+ + + + + + DV+R L +DRL D L LLSP AE LEE+A++A Sbjct: 3 TFYDVIEDYRHFDFAAYFAKVTDSDVRRILRQDRLSALDFLTLLSPQAEAYLEEMAQKAH 62 Query: 62 RLTRTHFGHVMQLFTPMYLADFCVNHCTYCSFSSIYDFPRKRLTPEEVRQEAIAIASTGL 121 RLT HFG M L+TP+YLA++CVN C YC F RK+LT EV QEA IA+TGL Sbjct: 63 RLTVQHFGRTMLLYTPLYLANYCVNQCVYCGFQLKNKLERKKLTLAEVEQEAQLIAATGL 122 Query: 122 RHLLVLTGESRSASPASYVKECVEILHEYFPSVSIEVNPLSTAEYRELREAGVDGLTLYQ 181 +H+L+LTGESR SP SY+K+CV IL +YF S+SIE+ PL+ EY EL AGVDGLT+YQ Sbjct: 123 KHILILTGESRQHSPVSYIKDCVNILKKYFSSISIEIYPLTQEEYAELIGAGVDGLTIYQ 182 Query: 182 EVYHQETYRKLHVKGPKRVFRNRLDAPERGCQAGFRAVNIGALLGMYDWRQEAFMTAMHA 241 EVY++E Y ++H GPKR +R RL+APER CQAG R VNIGALLG+ DWRQEAF T +HA Sbjct: 183 EVYNEEVYAEMHPAGPKRNYRFRLEAPERACQAGMRTVNIGALLGLNDWRQEAFFTGLHA 242 Query: 242 RYLQDKYPEXXXXLSAPRFRPYLGEFNPDSDVTDRALVQIILAYRLFLPRSGITLSTREP 301 YLQ ++P+ +S PR RP+LG F P V+D+ LVQ +LA+RLF+PRSGITLSTRE Sbjct: 243 DYLQRRFPDVEVSISPPRMRPHLGGFPPRVVVSDQNLVQYVLAFRLFMPRSGITLSTREN 302 Query: 302 APLRNHLIHLGITKMSAGVSTEVGGHTQDGGTPQFEISDERSVEEITDMLQANGLQPVFK 361 LR+ ++ LG+TKMSAG T VGG + QF+ISDER+V E+ ML A G QPV+K Sbjct: 303 GRLRDAMVRLGVTKMSAGSCTAVGGRSDQEAVGQFQISDERTVAEVAAMLYAQGYQPVYK 362 Query: 362 DWDLL 366 DW L Sbjct: 363 DWQAL 367 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,194,249,525 Number of extensions: 90064180 Number of successful extensions: 192573 Number of sequences better than 1.0e-05: 560 Number of HSP's gapped: 191541 Number of HSP's successfully gapped: 560 Length of query: 370 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 233 Effective length of database: 1,770,017,148 Effective search space: 412413995484 Effective search space used: 412413995484 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)