BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0017.p (557 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBQ1.1 B3KBQ1_9BACI Hydro-lyase, Fe-S type, tartrate/fumarate ... 535 e-150 Q9KEF8.1 Q9KEF8_BACHD Fumarate hydratase class I (Fumarase) 497 e-138 Q5L2W9.1 Q5L2W9_GEOKA Fumarate hydratase class I (Fumarase) EC=4... 486 e-135 A4IKC6.1 A4IKC6_GEOTN Fumarase 485 e-135 B4BQX4.1 B4BQX4_9BACI Hydro-lyase, Fe-S type, tartrate/fumarate ... 485 e-135 >B3KBQ1.1 B3KBQ1_9BACI Hydro-lyase, Fe-S type, tartrate/fumarate subfamily, alpha subunit Length = 517 Score = 535 bits (1379), Expect = e-150, Method: Compositional matrix adjust. Identities = 251/302 (83%), Positives = 278/302 (92%) Query: 1 MQHFEDSVYDLIVETSTNLPGDVRRAVAKGRALEDRATRSGLALTTIAQNIGMAELQVSP 60 M+ FE+S+Y LIVETSTNLPGDVRRAV +GRA ED ATR+GLAL TIAQNI MAE +VSP Sbjct: 1 MKLFEESMYRLIVETSTNLPGDVRRAVNRGRASEDAATRAGLALATIAQNIEMAEQEVSP 60 Query: 61 ICQDTGMPTFIIHTPVGVNQIEMKKDIHNAIIRATINGKLRPNSVDSLTGENSGNNLGAG 120 ICQDTGMPTFI+HTPVG N IEMKK I+ A++RAT +GKLRPNSVDSLTG NSG+NLG G Sbjct: 61 ICQDTGMPTFIVHTPVGANHIEMKKAIYAAVVRATKDGKLRPNSVDSLTGANSGDNLGPG 120 Query: 121 TPVIHFEQWEETGVDVRLILKGGGCENKNIQYSLPAELEGLGKAGRDLDGIRKCILHSVY 180 +PVIHFEQWE+ VDVRLILKGGGCENKNIQYSLPAELEGLGKAGRDLDGIRKCI+H+VY Sbjct: 121 SPVIHFEQWEQDKVDVRLILKGGGCENKNIQYSLPAELEGLGKAGRDLDGIRKCIMHAVY 180 Query: 181 QAQGQGCSAGFIGVGIGGDRTTGYELAKKQLFREVEDVNPNDDLAKLEEYIMENANKLGI 240 QAQGQGCSAGFIGVGIGGDRTTGYELAK QLFR VEDVNPN+DLAKLE+YI+ENANKLGI Sbjct: 181 QAQGQGCSAGFIGVGIGGDRTTGYELAKHQLFRHVEDVNPNEDLAKLEDYILENANKLGI 240 Query: 241 GTMGFGGEVTLLGCKIGVMNRLPASFFVSVAYNCWAFRRQGILVDPSTGHIQEWLYESGT 300 GTMGFGGEVTLLGCK+G MNRLPASFFVSVAYNCWAFRRQG+L++P TG I +WLYE+G+ Sbjct: 241 GTMGFGGEVTLLGCKVGAMNRLPASFFVSVAYNCWAFRRQGVLIEPETGEIADWLYEAGS 300 Query: 301 GI 302 + Sbjct: 301 EV 302 Score = 367 bits (943), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 170/191 (89%), Positives = 182/191 (95%) Query: 366 REVRLTTPVSEEDIRSLRVGDVVILSGEMHTGRDALHKYLMDHEAPVDLNGAVIYHCGPV 425 REV L TP+SEE IRSLRVGDVV++ GEMHTGRDALHKYLMDH+AP+DLNGAVIYHCGPV Sbjct: 326 REVVLQTPISEEQIRSLRVGDVVVIRGEMHTGRDALHKYLMDHDAPIDLNGAVIYHCGPV 385 Query: 426 MLKDEEGWHVKAAGPTTSIREEPYQGDIIKKFGIRAVIGKGGMGPKTLKALQEHGGVYLN 485 M++DEEGWHVKAAGPTTSIREEPYQGDI+KKFGIRAVIGKGGMGPKTL+ALQEHGGVYLN Sbjct: 386 MMQDEEGWHVKAAGPTTSIREEPYQGDILKKFGIRAVIGKGGMGPKTLQALQEHGGVYLN 445 Query: 486 AIGGAAQYYAECIKKVNGVDFMEFGIPEAMWHLQVDGFAAIVTMDAHGNSLHADVEKDSA 545 AIGGAAQYYA CIKKVNGVD MEFGIPEAMWHL+VDGFAAIVTMD+HGNSLHADV KDSA Sbjct: 446 AIGGAAQYYARCIKKVNGVDLMEFGIPEAMWHLEVDGFAAIVTMDSHGNSLHADVSKDSA 505 Query: 546 AKLAQFREPVF 556 KLA F+EPVF Sbjct: 506 QKLAMFKEPVF 516 >Q9KEF8.1 Q9KEF8_BACHD Fumarate hydratase class I (Fumarase) Length = 512 Score = 497 bits (1280), Expect = e-138, Method: Compositional matrix adjust. Identities = 232/299 (77%), Positives = 264/299 (88%) Query: 1 MQHFEDSVYDLIVETSTNLPGDVRRAVAKGRALEDRATRSGLALTTIAQNIGMAELQVSP 60 M+ ++S+Y LIVETSTNLP DVRRA+A+ +A E+ TR+ L+L+TI +NI MA+ +SP Sbjct: 1 MEKLQESMYQLIVETSTNLPNDVRRAIAEAKAKENAGTRAALSLSTITENIKMADENISP 60 Query: 61 ICQDTGMPTFIIHTPVGVNQIEMKKDIHNAIIRATINGKLRPNSVDSLTGENSGNNLGAG 120 ICQDTGMPTF I PVGVNQIEMKK I+ AI +AT +GKLRPNSVDSLTG+NSGNNLG G Sbjct: 61 ICQDTGMPTFEIKVPVGVNQIEMKKAIYAAIEQATKDGKLRPNSVDSLTGKNSGNNLGEG 120 Query: 121 TPVIHFEQWEETGVDVRLILKGGGCENKNIQYSLPAELEGLGKAGRDLDGIRKCILHSVY 180 TPVIHFEQWEE +D RLILKGGGCENKNIQYSLPAELEGLG+AGRDLDGIRKCI+HSVY Sbjct: 121 TPVIHFEQWEEDYIDARLILKGGGCENKNIQYSLPAELEGLGRAGRDLDGIRKCIMHSVY 180 Query: 181 QAQGQGCSAGFIGVGIGGDRTTGYELAKKQLFREVEDVNPNDDLAKLEEYIMENANKLGI 240 QAQGQGCSAGFIGVGIGGDRTT Y LAK+QLFR+V+DVNPNDDL KLEEY++ENANKLGI Sbjct: 181 QAQGQGCSAGFIGVGIGGDRTTSYSLAKEQLFRKVDDVNPNDDLRKLEEYVLENANKLGI 240 Query: 241 GTMGFGGEVTLLGCKIGVMNRLPASFFVSVAYNCWAFRRQGILVDPSTGHIQEWLYESG 299 GTMGFGGE TLLGCK+G MNRLPASFFVSVAYNCWAFRR G+ +D TG I++WLY+ G Sbjct: 241 GTMGFGGEATLLGCKVGAMNRLPASFFVSVAYNCWAFRRLGVELDAETGEIKKWLYKDG 299 Score = 330 bits (846), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 154/192 (80%), Positives = 173/192 (90%), Gaps = 1/192 (0%) Query: 366 REVRLTTPVSEEDIRSLRVGDVVILSGEMHTGRDALHKYLMDHEAPVDLNGAVIYHCGPV 425 REV L PV+EE IR L+VGDVVI++G +HTGRDA+H +LMDH+AP+DL+G +IYHCGPV Sbjct: 321 REVVLEAPVTEEQIRELKVGDVVIINGMLHTGRDAIHHHLMDHDAPIDLDGQIIYHCGPV 380 Query: 426 MLKDEEG-WHVKAAGPTTSIREEPYQGDIIKKFGIRAVIGKGGMGPKTLKALQEHGGVYL 484 ML+D+ G WHVKAAGPTTSIREEPYQGDI+KKFGIRAVIGKGGMGPKTLKALQEHGGVYL Sbjct: 381 MLQDDAGEWHVKAAGPTTSIREEPYQGDIMKKFGIRAVIGKGGMGPKTLKALQEHGGVYL 440 Query: 485 NAIGGAAQYYAECIKKVNGVDFMEFGIPEAMWHLQVDGFAAIVTMDAHGNSLHADVEKDS 544 NAIGGAAQYYA+CIKKV GVD MEFGIPEAMWHL+V+GFAAIVTMD+HGNSLH DV+K S Sbjct: 441 NAIGGAAQYYADCIKKVKGVDLMEFGIPEAMWHLEVEGFAAIVTMDSHGNSLHEDVDKSS 500 Query: 545 AAKLAQFREPVF 556 KL QF E VF Sbjct: 501 LEKLEQFAEKVF 512 >Q5L2W9.1 Q5L2W9_GEOKA Fumarate hydratase class I (Fumarase) EC=4.2.1.2 Length = 515 Score = 486 bits (1251), Expect = e-135, Method: Compositional matrix adjust. Identities = 229/302 (75%), Positives = 261/302 (86%) Query: 1 MQHFEDSVYDLIVETSTNLPGDVRRAVAKGRALEDRATRSGLALTTIAQNIGMAELQVSP 60 M+ F+ S+YDLIVETST LP DVRRA+A+ + E+ TR+ +AL TI +NI MA+ VSP Sbjct: 1 MEKFQQSMYDLIVETSTKLPKDVRRAIARAKVRENAGTRAAMALDTIVENIQMADENVSP 60 Query: 61 ICQDTGMPTFIIHTPVGVNQIEMKKDIHNAIIRATINGKLRPNSVDSLTGENSGNNLGAG 120 ICQDTG+PTF I PVGVNQIEMKK I AI+ AT NGKLRPNSVDSLTG+NSG+NLG G Sbjct: 61 ICQDTGLPTFKIKVPVGVNQIEMKKAIRAAIVEATKNGKLRPNSVDSLTGKNSGDNLGEG 120 Query: 121 TPVIHFEQWEETGVDVRLILKGGGCENKNIQYSLPAELEGLGKAGRDLDGIRKCILHSVY 180 PVI FEQWE +DVRLILKGGGCENKNIQYSLP ELEGLG+AGRDLDGIRKCILH+VY Sbjct: 121 VPVIKFEQWENDYIDVRLILKGGGCENKNIQYSLPCELEGLGRAGRDLDGIRKCILHAVY 180 Query: 181 QAQGQGCSAGFIGVGIGGDRTTGYELAKKQLFREVEDVNPNDDLAKLEEYIMENANKLGI 240 QAQGQGCSAGFIGVGIGGDR++GYELAK+QLFR V+DVNP ++L +LEEYIMENANKLGI Sbjct: 181 QAQGQGCSAGFIGVGIGGDRSSGYELAKEQLFRPVDDVNPIEELRQLEEYIMENANKLGI 240 Query: 241 GTMGFGGEVTLLGCKIGVMNRLPASFFVSVAYNCWAFRRQGILVDPSTGHIQEWLYESGT 300 GTMGFGGE TLLGCKIGVM+R+PASFFVSVAYNCWAFRR G+ +DP+TG I EWLY+ G Sbjct: 241 GTMGFGGESTLLGCKIGVMHRIPASFFVSVAYNCWAFRRMGVKIDPATGEIIEWLYQDGE 300 Query: 301 GI 302 + Sbjct: 301 DV 302 Score = 328 bits (840), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 152/194 (78%), Positives = 173/194 (89%), Gaps = 1/194 (0%) Query: 365 SREVRLTTPVSEEDIRSLRVGDVVILSGEMHTGRDALHKYLMDHEAPVDLNGAVIYHCGP 424 +R + L P+SEE +R LRVGDVV +SG ++TGRDA+HKYLMDH+APVDLNG +IYHCGP Sbjct: 322 TRVIDLVPPLSEEQVRQLRVGDVVRISGVIYTGRDAIHKYLMDHDAPVDLNGQIIYHCGP 381 Query: 425 VMLKDEEG-WHVKAAGPTTSIREEPYQGDIIKKFGIRAVIGKGGMGPKTLKALQEHGGVY 483 VMLKDEEG WH KAAGPTTSIREEPYQGDI+KKFG+RAVIGKGGMG KTL+AL+EHGGVY Sbjct: 382 VMLKDEEGRWHAKAAGPTTSIREEPYQGDIMKKFGVRAVIGKGGMGAKTLQALKEHGGVY 441 Query: 484 LNAIGGAAQYYAECIKKVNGVDFMEFGIPEAMWHLQVDGFAAIVTMDAHGNSLHADVEKD 543 LNAIGGAAQYYA+CIK V GVD +EFGIPEAMWHL+V+ F A+VTMD+HGNSLH DVEK Sbjct: 442 LNAIGGAAQYYADCIKSVEGVDLLEFGIPEAMWHLRVENFTAVVTMDSHGNSLHEDVEKS 501 Query: 544 SAAKLAQFREPVFK 557 S KLAQF+EPVFK Sbjct: 502 SLEKLAQFKEPVFK 515 >A4IKC6.1 A4IKC6_GEOTN Fumarase Length = 515 Score = 485 bits (1248), Expect = e-135, Method: Compositional matrix adjust. Identities = 229/302 (75%), Positives = 259/302 (85%) Query: 1 MQHFEDSVYDLIVETSTNLPGDVRRAVAKGRALEDRATRSGLALTTIAQNIGMAELQVSP 60 M F+ S+YDLIVETST LP DVRRA+A+ + E+ TR+ +AL TI +NI MA+ VSP Sbjct: 1 MDKFQQSMYDLIVETSTKLPKDVRRAIAQAKVRENLGTRAAMALDTIVENIQMADENVSP 60 Query: 61 ICQDTGMPTFIIHTPVGVNQIEMKKDIHNAIIRATINGKLRPNSVDSLTGENSGNNLGAG 120 ICQDTG+PTF I PVGVNQIEMKK I A++ AT NGKLRPNSVDSLTG+NSG+NLG G Sbjct: 61 ICQDTGLPTFKIKVPVGVNQIEMKKAIRAAVVEATKNGKLRPNSVDSLTGKNSGDNLGEG 120 Query: 121 TPVIHFEQWEETGVDVRLILKGGGCENKNIQYSLPAELEGLGKAGRDLDGIRKCILHSVY 180 PVI FEQWE +DVRLILKGGGCENKNIQYSLP ELEGLG+AGRDLDGIRKCILH+VY Sbjct: 121 VPVIKFEQWENDYIDVRLILKGGGCENKNIQYSLPCELEGLGRAGRDLDGIRKCILHAVY 180 Query: 181 QAQGQGCSAGFIGVGIGGDRTTGYELAKKQLFREVEDVNPNDDLAKLEEYIMENANKLGI 240 QAQGQGCSAGFIGVGIGGDR++GYELAK+QLFR V+DVNP ++L +LEEYIMENANKLGI Sbjct: 181 QAQGQGCSAGFIGVGIGGDRSSGYELAKEQLFRPVDDVNPIEELRQLEEYIMENANKLGI 240 Query: 241 GTMGFGGEVTLLGCKIGVMNRLPASFFVSVAYNCWAFRRQGILVDPSTGHIQEWLYESGT 300 GTMGFGGE TLLGCKIGVM+R+PASFFVSVAYNCWAFRR G+ +DP TG I EWLY+ G Sbjct: 241 GTMGFGGESTLLGCKIGVMHRIPASFFVSVAYNCWAFRRMGVKIDPQTGEIMEWLYQDGE 300 Query: 301 GI 302 I Sbjct: 301 DI 302 Score = 330 bits (845), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 153/194 (78%), Positives = 174/194 (89%), Gaps = 1/194 (0%) Query: 365 SREVRLTTPVSEEDIRSLRVGDVVILSGEMHTGRDALHKYLMDHEAPVDLNGAVIYHCGP 424 +R + L P+SEE +R LRVGDVV +SG ++TGRDA+HKYLMDH+APVDLNG +IYHCGP Sbjct: 322 TRVIDLVPPLSEEQVRQLRVGDVVRISGMIYTGRDAIHKYLMDHDAPVDLNGQIIYHCGP 381 Query: 425 VMLKDEEG-WHVKAAGPTTSIREEPYQGDIIKKFGIRAVIGKGGMGPKTLKALQEHGGVY 483 VMLKDEEG WHVKAAGPTTSIREEPYQGDI+KKFG+RAVIGKGGMG KTL+AL+EHGGVY Sbjct: 382 VMLKDEEGRWHVKAAGPTTSIREEPYQGDIMKKFGVRAVIGKGGMGAKTLQALKEHGGVY 441 Query: 484 LNAIGGAAQYYAECIKKVNGVDFMEFGIPEAMWHLQVDGFAAIVTMDAHGNSLHADVEKD 543 LNAIGGAAQYYA+CI+ V GVD MEFGIPEAMWHL+V+ F A+VTMD+HGNSLH DVEK Sbjct: 442 LNAIGGAAQYYADCIQSVEGVDLMEFGIPEAMWHLRVENFTAVVTMDSHGNSLHEDVEKS 501 Query: 544 SAAKLAQFREPVFK 557 S KLAQF+EPVFK Sbjct: 502 SLEKLAQFKEPVFK 515 >B4BQX4.1 B4BQX4_9BACI Hydro-lyase, Fe-S type, tartrate/fumarate subfamily, alpha subunit Length = 515 Score = 485 bits (1248), Expect = e-135, Method: Compositional matrix adjust. Identities = 229/302 (75%), Positives = 259/302 (85%) Query: 1 MQHFEDSVYDLIVETSTNLPGDVRRAVAKGRALEDRATRSGLALTTIAQNIGMAELQVSP 60 M F+ S+YDLIVETST LP DVRRA+A+ + E+ TR+ +AL TI +NI MA+ VSP Sbjct: 1 MDKFQQSMYDLIVETSTKLPKDVRRAIAQAKVRENLGTRAAMALDTIVENIQMADENVSP 60 Query: 61 ICQDTGMPTFIIHTPVGVNQIEMKKDIHNAIIRATINGKLRPNSVDSLTGENSGNNLGAG 120 ICQDTG+PTF I PVGVNQIEMKK I A++ AT NGKLRPNSVDSLTG+NSG+NLG G Sbjct: 61 ICQDTGLPTFKIKVPVGVNQIEMKKAIRAAVVEATKNGKLRPNSVDSLTGKNSGDNLGEG 120 Query: 121 TPVIHFEQWEETGVDVRLILKGGGCENKNIQYSLPAELEGLGKAGRDLDGIRKCILHSVY 180 PVI FEQWE +DVRLILKGGGCENKNIQYSLP ELEGLG+AGRDLDGIRKCILH+VY Sbjct: 121 VPVIKFEQWENDYIDVRLILKGGGCENKNIQYSLPCELEGLGRAGRDLDGIRKCILHAVY 180 Query: 181 QAQGQGCSAGFIGVGIGGDRTTGYELAKKQLFREVEDVNPNDDLAKLEEYIMENANKLGI 240 QAQGQGCSAGFIGVGIGGDR++GYELAK+QLFR V+DVNP ++L +LEEYIMENANKLGI Sbjct: 181 QAQGQGCSAGFIGVGIGGDRSSGYELAKEQLFRPVDDVNPIEELRQLEEYIMENANKLGI 240 Query: 241 GTMGFGGEVTLLGCKIGVMNRLPASFFVSVAYNCWAFRRQGILVDPSTGHIQEWLYESGT 300 GTMGFGGE TLLGCKIGVM+R+PASFFVSVAYNCWAFRR G+ +DP TG I EWLY+ G Sbjct: 241 GTMGFGGESTLLGCKIGVMHRIPASFFVSVAYNCWAFRRMGVKIDPQTGEIMEWLYQDGE 300 Query: 301 GI 302 I Sbjct: 301 DI 302 Score = 330 bits (845), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 153/194 (78%), Positives = 174/194 (89%), Gaps = 1/194 (0%) Query: 365 SREVRLTTPVSEEDIRSLRVGDVVILSGEMHTGRDALHKYLMDHEAPVDLNGAVIYHCGP 424 +R + L P+SEE +R LRVGDVV +SG ++TGRDA+HKYLMDH+APVDLNG +IYHCGP Sbjct: 322 TRVIDLVPPLSEEQVRQLRVGDVVRISGMIYTGRDAIHKYLMDHDAPVDLNGQIIYHCGP 381 Query: 425 VMLKDEEG-WHVKAAGPTTSIREEPYQGDIIKKFGIRAVIGKGGMGPKTLKALQEHGGVY 483 VMLKDEEG WHVKAAGPTTSIREEPYQGDI+KKFG+RAVIGKGGMG KTL+AL+EHGGVY Sbjct: 382 VMLKDEEGRWHVKAAGPTTSIREEPYQGDIMKKFGVRAVIGKGGMGAKTLQALKEHGGVY 441 Query: 484 LNAIGGAAQYYAECIKKVNGVDFMEFGIPEAMWHLQVDGFAAIVTMDAHGNSLHADVEKD 543 LNAIGGAAQYYA+CI+ V GVD MEFGIPEAMWHL+V+ F A+VTMD+HGNSLH DVEK Sbjct: 442 LNAIGGAAQYYADCIQSVEGVDLMEFGIPEAMWHLRVENFTAVVTMDSHGNSLHEDVEKS 501 Query: 544 SAAKLAQFREPVFK 557 S KLAQF+EPVFK Sbjct: 502 SLEKLAQFKEPVFK 515 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.138 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,170,852,974 Number of extensions: 138713295 Number of successful extensions: 323947 Number of sequences better than 1.0e-05: 1560 Number of HSP's gapped: 320231 Number of HSP's successfully gapped: 1955 Length of query: 557 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 416 Effective length of database: 1,732,333,088 Effective search space: 720650564608 Effective search space used: 720650564608 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)