BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0008.p (228 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBR0.1 B3KBR0_9BACI Phosphate transport system regulatory prot... 327 5e-88 B1D706.1 B1D706_9BACL Phosphate uptake regulator, PhoU 261 3e-68 A9U6U5.1 A9U6U5_PHYPA Predicted protein (Fragment) 258 2e-67 B8G180.1 B8G180_DESHD Phosphate uptake regulator, PhoU 211 3e-53 Q3AAH0.1 Q3AAH0_CARHZ Phosphate transport system regulatory prot... 206 2e-51 >B3KBR0.1 B3KBR0_9BACI Phosphate transport system regulatory protein PhoU Length = 219 Score = 327 bits (838), Expect = 5e-88, Method: Compositional matrix adjust. Identities = 154/219 (70%), Positives = 192/219 (87%) Query: 10 MIRRKEFDKDLEELRSLLQQMGEHVTDALEGAILALQTLDTARAQEIVKADLRLNAMEDR 69 MIRR EFD+ L+ELRSLLQ MG+H+ +AL ++ +LQTLD +AQ ++K D++LN ME++ Sbjct: 1 MIRRIEFDQQLDELRSLLQDMGKHMEEALANSVESLQTLDAEKAQAVIKNDVQLNNMEEQ 60 Query: 70 IMEIGSRLIITQQPVAKDLRRIIVAFKISSDLERMGDLALDVAKVTMRVQGQQLIKPLVD 129 IM+IGSRLI+TQQPVAKDLRRI+VAF+I+SDLERMGDLA DVAKVT+R+QGQ+LIKPLVD Sbjct: 61 IMDIGSRLIVTQQPVAKDLRRILVAFRIASDLERMGDLARDVAKVTIRLQGQKLIKPLVD 120 Query: 130 IPRMAEVVTGMITEAIQSYLDENTDLAYKMAQDDDQVDHLYSGMINELYAFMVEKPECLN 189 +PRM+++V M++E+I+SYL ENTDLAYKMAQDDDQVDHLYS ++ +LY+ + P+ N Sbjct: 121 LPRMSDMVRAMVSESIESYLTENTDLAYKMAQDDDQVDHLYSVILQDLYSHLTSDPDSAN 180 Query: 190 QAMLLTLVGRYIERIADHATNIGESVVYLVTGKRPDLNQ 228 QAMLL +VGRYIERIADHATNIGES VYLVTG RPDLNQ Sbjct: 181 QAMLLIMVGRYIERIADHATNIGESTVYLVTGHRPDLNQ 219 >B1D706.1 B1D706_9BACL Phosphate uptake regulator, PhoU Length = 224 Score = 261 bits (668), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 128/219 (58%), Positives = 166/219 (75%) Query: 9 IMIRRKEFDKDLEELRSLLQQMGEHVTDALEGAILALQTLDTARAQEIVKADLRLNAMED 68 +M RKEFD LE+L +LL +MG V +AL ++ AL DT +A+ I+ D LN +ED Sbjct: 1 MMTTRKEFDHGLEQLHNLLVEMGTFVEEALVDSMKALSGTDTEKARAIINNDQHLNNLED 60 Query: 69 RIMEIGSRLIITQQPVAKDLRRIIVAFKISSDLERMGDLALDVAKVTMRVQGQQLIKPLV 128 +I ++GS+LI TQQPVAKDLRRI+ AFKI+SDLERMGDL +++AK +R++GQ LIKPL+ Sbjct: 61 KITDLGSKLIATQQPVAKDLRRILAAFKIASDLERMGDLCVNIAKFVIRLEGQPLIKPLI 120 Query: 129 DIPRMAEVVTGMITEAIQSYLDENTDLAYKMAQDDDQVDHLYSGMINELYAFMVEKPECL 188 D+PRMAE+V M E+IQS++ EN DLAYK+A +DDQVD L + + EL + M+E P + Sbjct: 121 DLPRMAEIVQTMTYESIQSFVQENVDLAYKLATEDDQVDALCAQISRELLSLMMENPRNI 180 Query: 189 NQAMLLTLVGRYIERIADHATNIGESVVYLVTGKRPDLN 227 +QA +L V R IERI DHATNIGESVVYLVT KRPDLN Sbjct: 181 SQATVLNFVARNIERIGDHATNIGESVVYLVTNKRPDLN 219 >A9U6U5.1 A9U6U5_PHYPA Predicted protein (Fragment) Length = 610 Score = 258 bits (660), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 124/184 (67%), Positives = 152/184 (82%) Query: 44 ALQTLDTARAQEIVKADLRLNAMEDRIMEIGSRLIITQQPVAKDLRRIIVAFKISSDLER 103 +L ++ A +I++ D LN +E++I E+GS+LI TQQPVAKDLRRI+VAF+IS DLER Sbjct: 3 SLIHINAKEAAQIIQEDPELNRLEEKITELGSKLIATQQPVAKDLRRILVAFRISGDLER 62 Query: 104 MGDLALDVAKVTMRVQGQQLIKPLVDIPRMAEVVTGMITEAIQSYLDENTDLAYKMAQDD 163 MGDL++D+AKV MR+ GQ+LIKPL+D+PRMAE V MI E+IQSY+ EN DLAYKMA+DD Sbjct: 63 MGDLSVDIAKVVMRLDGQELIKPLIDLPRMAETVQTMIFESIQSYVQENVDLAYKMAKDD 122 Query: 164 DQVDHLYSGMINELYAFMVEKPECLNQAMLLTLVGRYIERIADHATNIGESVVYLVTGKR 223 D VD LYS +I E++ MVE P +NQAMLL+ VGRYIERIADHATNIGESV YLVTGKR Sbjct: 123 DIVDALYSQIIREMFTIMVENPRTINQAMLLSFVGRYIERIADHATNIGESVTYLVTGKR 182 Query: 224 PDLN 227 PDLN Sbjct: 183 PDLN 186 >B8G180.1 B8G180_DESHD Phosphate uptake regulator, PhoU Length = 219 Score = 211 bits (538), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 107/219 (48%), Positives = 148/219 (67%) Query: 10 MIRRKEFDKDLEELRSLLQQMGEHVTDALEGAILALQTLDTARAQEIVKADLRLNAMEDR 69 M R+ FDK LEEL + ++G+ V ++ AI +L D A ++ DL +N ++ Sbjct: 1 MANRQMFDKSLEELNEKVFELGKKVNSRVDAAIKSLAKQDLELADNVITGDLEINELQAD 60 Query: 70 IMEIGSRLIITQQPVAKDLRRIIVAFKISSDLERMGDLALDVAKVTMRVQGQQLIKPLVD 129 I E LI QQP AKDLR+I+ AFKIS +LER+GDL++DVAK T+R GQ+L+KPLVD Sbjct: 61 IEETCMLLIAQQQPFAKDLRKIVAAFKISINLERIGDLSVDVAKHTLRTGGQELLKPLVD 120 Query: 130 IPRMAEVVTGMITEAIQSYLDENTDLAYKMAQDDDQVDHLYSGMINELYAFMVEKPECLN 189 +PRMA++V M+ + I++Y E+ A MA DD+VDHLY+ + EL FM+E P+ +N Sbjct: 121 LPRMADLVQQMVIKGIEAYKTEDGSAAKAMALMDDEVDHLYAQVFRELLVFMMEAPKTIN 180 Query: 190 QAMLLTLVGRYIERIADHATNIGESVVYLVTGKRPDLNQ 228 QA L VGR++ER+ D+ TNI E VVY+ TGKR DLNQ Sbjct: 181 QATYLIFVGRFLERMGDYCTNIAEEVVYVETGKRSDLNQ 219 >Q3AAH0.1 Q3AAH0_CARHZ Phosphate transport system regulatory protein PhoU Length = 219 Score = 206 bits (523), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 107/218 (49%), Positives = 146/218 (66%) Query: 10 MIRRKEFDKDLEELRSLLQQMGEHVTDALEGAILALQTLDTARAQEIVKADLRLNAMEDR 69 M R+ FDK L EL + + +MG V A+ A+ +L D AQ ++ D +N +E Sbjct: 1 MTARQNFDKALNELENEILRMGSMVERAIYDAVESLAKQDVDLAQRVINGDRDINYLETE 60 Query: 70 IMEIGSRLIITQQPVAKDLRRIIVAFKISSDLERMGDLALDVAKVTMRVQGQQLIKPLVD 129 I + LI TQQP+A+DLRRII KI ++LERMGD A+D+A+VT R+ GQ LIKPLVD Sbjct: 61 IEDRLVTLIATQQPIARDLRRIITGIKIITNLERMGDHAVDIARVTKRIAGQPLIKPLVD 120 Query: 130 IPRMAEVVTGMITEAIQSYLDENTDLAYKMAQDDDQVDHLYSGMINELYAFMVEKPECLN 189 IPRMA++ M+ E + +Y+ + D A M DD+VDHLYS + EL +M+E P+ + Sbjct: 121 IPRMAKISQQMVKEGLDAYVRSDVDKAKYMCSLDDEVDHLYSQVFRELLTYMMEDPKTIA 180 Query: 190 QAMLLTLVGRYIERIADHATNIGESVVYLVTGKRPDLN 227 QA L V R++ERIAD+ATNIGE+V+YLVTG+R DLN Sbjct: 181 QATSLLFVARFLERIADYATNIGEAVIYLVTGERFDLN 218 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.137 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,120,692,295 Number of extensions: 40629597 Number of successful extensions: 142393 Number of sequences better than 1.0e-05: 1413 Number of HSP's gapped: 140713 Number of HSP's successfully gapped: 1413 Length of query: 228 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 97 Effective length of database: 1,826,543,238 Effective search space: 177174694086 Effective search space used: 177174694086 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)