BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0005.p (280 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBR2.1 B3KBR2_9BACI DNA glycosylase EC=4.2.99.18 385 e-105 B1D702.1 B1D702_9BACL DNA glycosylase EC=4.2.99.18 355 2e-96 C0Z7Z0.1 FPG_BREBN Formamidopyrimidine-DNA glycosylase EC=3.2.2.... 337 9e-91 P19210.3 FPG_BACFI Formamidopyrimidine-DNA glycosylase EC=3.2.2.... 324 6e-87 Q65G93.3 FPG_BACLD Formamidopyrimidine-DNA glycosylase EC=3.2.2.... 321 5e-86 >B3KBR2.1 B3KBR2_9BACI DNA glycosylase EC=4.2.99.18 Length = 277 Score = 385 bits (988), Expect = e-105, Method: Compositional matrix adjust. Identities = 188/274 (68%), Positives = 213/274 (77%) Query: 1 MPELPEVETVKRTLNALITGKQIQNVTVRLPRIIQRPDDIQAFANMLAGHTVVQVERRGK 60 MPELPEVETVKRTLN LI GK I +V+V LPRIIQRPDDI+AFA ML GHTV VERRGK Sbjct: 1 MPELPEVETVKRTLNQLIKGKHIDHVSVNLPRIIQRPDDIEAFAFMLKGHTVEGVERRGK 60 Query: 61 FLRFVLDGLVMVSHLRMEGRYGLYRGEEPLDKHTHVIFHFTDGTELRYTDVRQFGTMHLF 120 FLR +LDGLV+VSHLRMEGRYG+YR EEP++KHTHVIFHF+DGTELRY DVRQFGTMHLF Sbjct: 61 FLRILLDGLVLVSHLRMEGRYGVYRAEEPVEKHTHVIFHFSDGTELRYKDVRQFGTMHLF 120 Query: 121 QXXXXXXXXXXXXXXXXXXAPSFTPERFKEIVSGKSTKIKPLLLNQEYVVGIGNIYVDEA 180 +FT FKE++ K TKIK +LLNQ YVVGIGNIYVDEA Sbjct: 121 APGEEFQHAPLAKLGYEPLDDTFTFGTFKEVIGTKKTKIKAVLLNQAYVVGIGNIYVDEA 180 Query: 181 LHRARIHPETGAKALTSSQLDLLHHAIVATLTEAVNAGGSSVKSYVNGQGESGSYQHQLL 240 L RA+IHPE A +L ++L L+HAIV TL+EAV+AGGSS+KSYVNGQGE G +Q+ Sbjct: 181 LFRAKIHPERNANSLKDAELKRLYHAIVDTLSEAVDAGGSSIKSYVNGQGEMGMFQNSHQ 240 Query: 241 IYGRKDQPCASCGTLIEKTVVGGRGTHYCPSCQP 274 IYGRKD+PC +CG I K VVGGRGTHYCP CQP Sbjct: 241 IYGRKDKPCHACGGPIHKIVVGGRGTHYCPKCQP 274 >B1D702.1 B1D702_9BACL DNA glycosylase EC=4.2.99.18 Length = 287 Score = 355 bits (911), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 174/275 (63%), Positives = 208/275 (75%), Gaps = 1/275 (0%) Query: 1 MPELPEVETVKRTLNALITGKQIQNVTVRLPRIIQRPDDIQAFANMLAGHTVVQVERRGK 60 MPELPEVETV+RTL L+ GK+I +V V+LPRIIQRP + +AF LAG T+ VERRGK Sbjct: 1 MPELPEVETVRRTLIELVAGKRIASVEVKLPRIIQRPAEPEAFEMALAGRTIQTVERRGK 60 Query: 61 FLRFVLDGLVMVSHLRMEGRYGLYRGEEPLDKHTHVIFHFTDGTELRYTDVRQFGTMHLF 120 FLR V+DGLV+VSHLRMEGRYG++ +EP++ HTHV+FHF DGT+LRY DVRQFGTMHLF Sbjct: 61 FLRVVMDGLVLVSHLRMEGRYGVFPSDEPVELHTHVLFHFDDGTDLRYKDVRQFGTMHLF 120 Query: 121 QXXXXXXXXXXXXX-XXXXXAPSFTPERFKEIVSGKSTKIKPLLLNQEYVVGIGNIYVDE 179 + +FT + K ++ K+ IKPLLLNQEYVVG+GNIYVDE Sbjct: 121 EPGTEFEMKPLSKLGLEPLENEAFTADALKGLLGKKTGLIKPLLLNQEYVVGLGNIYVDE 180 Query: 180 ALHRARIHPETGAKALTSSQLDLLHHAIVATLTEAVNAGGSSVKSYVNGQGESGSYQHQL 239 AL A IHPE A +L ++ LH AIV+TL +AV AGGSS+KSYVNGQGE G +QHQL Sbjct: 181 ALFLAGIHPERTADSLKKAEWIRLHEAIVSTLAKAVAAGGSSIKSYVNGQGEMGMFQHQL 240 Query: 240 LIYGRKDQPCASCGTLIEKTVVGGRGTHYCPSCQP 274 L+YGRKD+PCASCG LIEK+VVGGRGTH C SCQP Sbjct: 241 LVYGRKDEPCASCGRLIEKSVVGGRGTHTCASCQP 275 >C0Z7Z0.1 FPG_BREBN Formamidopyrimidine-DNA glycosylase EC=3.2.2.23 EC=4.2.99.18 Length = 276 Score = 337 bits (863), Expect = 9e-91, Method: Compositional matrix adjust. Identities = 159/273 (58%), Positives = 200/273 (73%) Query: 1 MPELPEVETVKRTLNALITGKQIQNVTVRLPRIIQRPDDIQAFANMLAGHTVVQVERRGK 60 MPELPEVETV RTL L+ GK I+ V+V L RI+++PDD++AF ++L G T+ ++RR K Sbjct: 1 MPELPEVETVVRTLRGLVMGKTIERVSVHLARIVRQPDDVEAFKSLLVGQTIQDIQRRAK 60 Query: 61 FLRFVLDGLVMVSHLRMEGRYGLYRGEEPLDKHTHVIFHFTDGTELRYTDVRQFGTMHLF 120 F++F L+ V+VSHLRMEGRYG+Y+ ++P++KHTHV+FHFTDGTELRY DVRQFGTM LF Sbjct: 61 FIQFFLNEDVLVSHLRMEGRYGVYQADDPVEKHTHVVFHFTDGTELRYRDVRQFGTMDLF 120 Query: 121 QXXXXXXXXXXXXXXXXXXAPSFTPERFKEIVSGKSTKIKPLLLNQEYVVGIGNIYVDEA 180 SFTPE +++ G+STKIKPLLLNQE +VG+GNIYVDE+ Sbjct: 121 PKGKETTIGPLAKLGVEPLDKSFTPEVLGKLLKGRSTKIKPLLLNQECIVGLGNIYVDES 180 Query: 181 LHRARIHPETGAKALTSSQLDLLHHAIVATLTEAVNAGGSSVKSYVNGQGESGSYQHQLL 240 L +A IHPE A LT ++ LH +IV+TL EAV GGSS+KSYVNGQGE G +Q LL Sbjct: 181 LFKAGIHPEKPAGKLTDKEVIRLHESIVSTLQEAVEQGGSSIKSYVNGQGEMGMFQQSLL 240 Query: 241 IYGRKDQPCASCGTLIEKTVVGGRGTHYCPSCQ 273 +YGRKD+ C CG I + VVGGRGTH CP CQ Sbjct: 241 VYGRKDEACTKCGAEIIRFVVGGRGTHICPDCQ 273 >P19210.3 FPG_BACFI Formamidopyrimidine-DNA glycosylase EC=3.2.2.23 EC=4.2.99.18 Length = 274 Score = 324 bits (830), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 159/273 (58%), Positives = 188/273 (68%) Query: 1 MPELPEVETVKRTLNALITGKQIQNVTVRLPRIIQRPDDIQAFANMLAGHTVVQVERRGK 60 MPELPEVETVKRTL L+ GK I +TV+ II+ P D+ F +L T+ + RRGK Sbjct: 1 MPELPEVETVKRTLTELVIGKTIAGITVKWANIIKEPADVLEFETLLMNQTIRSIRRRGK 60 Query: 61 FLRFVLDGLVMVSHLRMEGRYGLYRGEEPLDKHTHVIFHFTDGTELRYTDVRQFGTMHLF 120 FL F D +VMVSHLRMEGRYGLY EEPL HTHVIFHFTDG ELRY DVR+FGTMHLF Sbjct: 61 FLLFEFDDIVMVSHLRMEGRYGLYEKEEPLPPHTHVIFHFTDGEELRYQDVRKFGTMHLF 120 Query: 121 QXXXXXXXXXXXXXXXXXXAPSFTPERFKEIVSGKSTKIKPLLLNQEYVVGIGNIYVDEA 180 + FT E + KIK LL+Q+ VVG+GNIYVDEA Sbjct: 121 PKGSEEKVLPLAHLGVEPFSEQFTSELLMNAFQKTNRKIKVALLDQKTVVGLGNIYVDEA 180 Query: 181 LHRARIHPETGAKALTSSQLDLLHHAIVATLTEAVNAGGSSVKSYVNGQGESGSYQHQLL 240 L RARIHPE A +L+ ++ +LH AIV+TL EAV GGSS+KSYVNGQGE G +Q +L Sbjct: 181 LFRARIHPERLAHSLSKEEMAVLHKAIVSTLEEAVEMGGSSIKSYVNGQGEMGMFQQKLG 240 Query: 241 IYGRKDQPCASCGTLIEKTVVGGRGTHYCPSCQ 273 +YGRK++PC CGT I KTVVGGRGTH+CP+CQ Sbjct: 241 VYGRKNEPCRQCGTDILKTVVGGRGTHFCPNCQ 273 >Q65G93.3 FPG_BACLD Formamidopyrimidine-DNA glycosylase EC=3.2.2.23 EC=4.2.99.18 Length = 275 Score = 321 bits (822), Expect = 5e-86, Method: Compositional matrix adjust. Identities = 156/275 (56%), Positives = 192/275 (69%) Query: 1 MPELPEVETVKRTLNALITGKQIQNVTVRLPRIIQRPDDIQAFANMLAGHTVVQVERRGK 60 MPELPEVETV+RTL L+ GK I V VR +II+RP++ + FA +LAG T+ + RRGK Sbjct: 1 MPELPEVETVRRTLAGLVRGKTIDAVDVRWTKIIKRPEEPEEFARLLAGQTIQSIGRRGK 60 Query: 61 FLRFVLDGLVMVSHLRMEGRYGLYRGEEPLDKHTHVIFHFTDGTELRYTDVRQFGTMHLF 120 FL F LD VMVSHLRMEG+YGL++ +EPLDKH HVIF FTDG+ELRY DVR+FGTMHLF Sbjct: 61 FLLFHLDDCVMVSHLRMEGKYGLHQNDEPLDKHVHVIFRFTDGSELRYRDVRKFGTMHLF 120 Query: 121 QXXXXXXXXXXXXXXXXXXAPSFTPERFKEIVSGKSTKIKPLLLNQEYVVGIGNIYVDEA 180 + + FT + +E + + +K LL+Q VVG+GNIYVDEA Sbjct: 121 KPGEELTELPLRQLGPEPFSSEFTADYLRERLKKTNRSVKTALLDQRTVVGLGNIYVDEA 180 Query: 181 LHRARIHPETGAKALTSSQLDLLHHAIVATLTEAVNAGGSSVKSYVNGQGESGSYQHQLL 240 L RA IHPE A LT Q LLH I+ TL EAV AGGS+V+SY+N QGE G +Q +L Sbjct: 181 LFRAGIHPEATANKLTKKQTVLLHKEIIQTLKEAVEAGGSTVRSYINSQGEIGMFQLKLF 240 Query: 241 IYGRKDQPCASCGTLIEKTVVGGRGTHYCPSCQPQ 275 +YGRKD+PC CG+ IEKTVVGGRGTH+C CQ + Sbjct: 241 VYGRKDEPCKKCGSPIEKTVVGGRGTHFCIKCQKK 275 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,554,550,358 Number of extensions: 59510694 Number of successful extensions: 147980 Number of sequences better than 1.0e-05: 1559 Number of HSP's gapped: 144735 Number of HSP's successfully gapped: 1593 Length of query: 280 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 146 Effective length of database: 1,798,280,193 Effective search space: 262548908178 Effective search space used: 262548908178 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)