BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0004.p (253 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBR3.1 B3KBR3_9BACI Sporulation protein YtaF 135 5e-30 B1D701.1 B1D701_9BACL Sporulation protein YtaF 112 2e-23 Q633L2.1 Q633L2_BACCZ Putative uncharacterized protein ytaF 85 6e-15 C3ERI0.1 C3ERI0_BACTK Putative uncharacterized protein 76 4e-12 C3FRE6.1 C3FRE6_BACTB Putative uncharacterized protein 76 4e-12 >B3KBR3.1 B3KBR3_9BACI Sporulation protein YtaF Length = 246 Score = 135 bits (339), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 88/235 (37%), Positives = 114/235 (48%), Gaps = 7/235 (2%) Query: 20 DGFGVGITYGLRKMKXXXXXXXXXXXXXXXXXYVSMQVGVLLAKVVSPHAASSIGAVILV 79 DGFG G+TYGLRK + VSMQ G LL + +SP AAS IGA IL+ Sbjct: 18 DGFGAGVTYGLRKTRIPLLSVLIISLCSGLVLGVSMQAGALLQRFLSPSAASVIGAAILI 77 Query: 80 LMGCWSLFQMLTQKEKDHDGKSPGDQHGRA-DHDXXXXXXXXXXXXXXXXXXXXXXXXXS 138 L+G WSL+Q + + + P Q A D Sbjct: 78 LLGLWSLYQQVRRTSE------PEVQPASAVDSSASLTETPGAIHQGTPRREVTTAGQEQ 131 Query: 139 PKSAVFSLELRHLGVVIQILRTPSSADMDASGSISSTEAMVLGIXXXXXXXXXXXXXXXX 198 + AVFSLE+ +GVVIQILR+P+ AD+D SGSIS EAM LGI Sbjct: 132 LQRAVFSLEIPKIGVVIQILRSPTKADLDDSGSISPWEAMWLGIALSLDAFGAGLGAAML 191 Query: 199 XXXPVSTSLMIALFSGTFLLLGMKTGLKLSGSYWMKHXXXXXXXXXXXMGIMKLL 253 P+ T+ +++LFSG FL+LGM+ GL+ + M+ MGIMKLL Sbjct: 192 GFSPLGTAAVVSLFSGIFLVLGMRVGLRFAAKGGMRFISYLPAFLLVVMGIMKLL 246 >B1D701.1 B1D701_9BACL Sporulation protein YtaF Length = 234 Score = 112 bits (281), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 73/215 (33%), Positives = 104/215 (48%), Gaps = 17/215 (7%) Query: 20 DGFGVGITYGLRKMKXXXXXXXXXXXXXXXXXYVSMQVGVLLAKVVSPHAASSIGAVILV 79 DGFGVG+TYGLR+++ ++SM VG + +SP A IGAV+LV Sbjct: 18 DGFGVGVTYGLRRIRIPLLSVVIIACCSGLVIFLSMIVGSWITGYLSPFVAKLIGAVVLV 77 Query: 80 LMGCWSLFQMLTQKEKDHDGKSPGDQHGRADHDXXXXXXXXXXXXXXXXXXXXXXXXXSP 139 +G W+L Q+ ++ G + G G ADH + Sbjct: 78 GIGTWALLQL--RRSGREAGAAEGQAEGDADHSAGSDQEQIDR---------------TH 120 Query: 140 KSAVFSLELRHLGVVIQILRTPSSADMDASGSISSTEAMVLGIXXXXXXXXXXXXXXXXX 199 V +EL+ LG+VIQILRTP +AD+D SG+IS+TEA++LG+ Sbjct: 121 PVLVVIVELKRLGLVIQILRTPQAADVDKSGTISATEALMLGVALSFDAFGAGIGAAMIG 180 Query: 200 XXPVSTSLMIALFSGTFLLLGMKTGLKLSGSYWMK 234 P+ T +MIA S FLL GM+ G + S M+ Sbjct: 181 LSPLLTPIMIAAASALFLLGGMRFGFRFSNWKGMR 215 >Q633L2.1 Q633L2_BACCZ Putative uncharacterized protein ytaF Length = 210 Score = 84.7 bits (208), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 64/233 (27%), Positives = 99/233 (42%), Gaps = 39/233 (16%) Query: 20 DGFGVGITYGLRKMKXXXXXXXXXXXXXXXXXYVSMQVGVLLAKVVSPHAASSIGAVILV 79 D VG+TYGLR ++ VSM +G ++AK+ SP A+ IG ++L+ Sbjct: 17 DSCSVGLTYGLRSVRIPLRSIVIIGMCSAAVMLVSMGIGHMIAKIFSPVIATRIGGLVLI 76 Query: 80 LMGCWSLFQMLTQKEKDHDGKSPGDQHGRADHDXXXXXXXXXXXXXXXXXXXXXXXXXSP 139 +G W L+Q ++K+ Sbjct: 77 GIGIWVLYQFFRSEKKEEP---------------------------------------KQ 97 Query: 140 KSAVFSLELRHLGVVIQILRTPSSADMDASGSISSTEAMVLGIXXXXXXXXXXXXXXXXX 199 + V+ LE+ LG+VIQILR P+ AD D SG+IS+ EA++LGI Sbjct: 98 EEKVWKLEIASLGLVIQILRKPTVADFDKSGTISAGEALLLGIALSVDSFGAGIGASLLG 157 Query: 200 XXPVSTSLMIALFSGTFLLLGMKTGLKLSGSYWMKHXXXXXXXXXXXMGIMKL 252 P ++++A+ S FL +GMK G LS W++ +GI K+ Sbjct: 158 YTPAMMAILVAMMSSLFLFIGMKLGTVLSNMKWLQKFTFLPGVLLIIIGIWKM 210 >C3ERI0.1 C3ERI0_BACTK Putative uncharacterized protein Length = 169 Score = 75.9 bits (185), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 57/200 (28%), Positives = 88/200 (44%), Gaps = 39/200 (19%) Query: 53 VSMQVGVLLAKVVSPHAASSIGAVILVLMGCWSLFQMLTQKEKDHDGKSPGDQHGRADHD 112 VSM +G ++AKV SP A+ IG ++L+ +G W L+Q ++K+ Sbjct: 9 VSMGIGHMIAKVFSPVIATRIGGLVLIGIGIWVLYQFFRSEKKEEP-------------- 54 Query: 113 XXXXXXXXXXXXXXXXXXXXXXXXXSPKSAVFSLELRHLGVVIQILRTPSSADMDASGSI 172 + V+ LE+ LG+VIQILR P+ AD D SG+I Sbjct: 55 -------------------------KQEEKVWKLEIASLGLVIQILRKPTVADFDKSGTI 89 Query: 173 SSTEAMVLGIXXXXXXXXXXXXXXXXXXXPVSTSLMIALFSGTFLLLGMKTGLKLSGSYW 232 S+ EA++LGI P ++++A+ S FL +GMK G LS W Sbjct: 90 SAGEALLLGIALSVDSFGAGIGASLLGYVPAMMAILVAIMSSLFLFIGMKLGTVLSNMKW 149 Query: 233 MKHXXXXXXXXXXXMGIMKL 252 ++ +GI K+ Sbjct: 150 LQKFTFLPGVLLIIIGIWKM 169 >C3FRE6.1 C3FRE6_BACTB Putative uncharacterized protein Length = 169 Score = 75.9 bits (185), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 57/200 (28%), Positives = 88/200 (44%), Gaps = 39/200 (19%) Query: 53 VSMQVGVLLAKVVSPHAASSIGAVILVLMGCWSLFQMLTQKEKDHDGKSPGDQHGRADHD 112 VSM +G ++AKV SP A+ IG ++L+ +G W L+Q ++K+ Sbjct: 9 VSMGIGHMIAKVFSPVIATRIGGLVLIGIGIWVLYQFFRSEKKEEP-------------- 54 Query: 113 XXXXXXXXXXXXXXXXXXXXXXXXXSPKSAVFSLELRHLGVVIQILRTPSSADMDASGSI 172 + V+ LE+ LG+VIQILR P+ AD D SG+I Sbjct: 55 -------------------------KQEEKVWKLEIASLGLVIQILRKPTVADFDKSGTI 89 Query: 173 SSTEAMVLGIXXXXXXXXXXXXXXXXXXXPVSTSLMIALFSGTFLLLGMKTGLKLSGSYW 232 S+ EA++LGI P ++++A+ S FL +GMK G LS W Sbjct: 90 SAGEALLLGIALSVDSFGAGIGASLLGYAPAMMAILVAIMSSLFLFIGMKLGTVLSNMKW 149 Query: 233 MKHXXXXXXXXXXXMGIMKL 252 ++ +GI K+ Sbjct: 150 LQKFTFLPGVLLIIIGIWKM 169 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 849,662,190 Number of extensions: 20949621 Number of successful extensions: 46944 Number of sequences better than 1.0e-05: 90 Number of HSP's gapped: 46874 Number of HSP's successfully gapped: 97 Length of query: 253 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 120 Effective length of database: 1,807,701,208 Effective search space: 216924144960 Effective search space used: 216924144960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)