BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0270.0003.p (299 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DA09.1 B1DA09_9BACL Metallophosphoesterase 367 e-100 B3K862.1 B3K862_9BACI Metallophosphoesterase 299 3e-79 B3KD47.1 B3KD47_9BACI Metallophosphoesterase 276 1e-72 A6LGC4.1 A6LGC4_PARD8 Putative Icc family phosphohydrolase 265 4e-69 C3QH80.1 C3QH80_9BACE Icc family phosphohydrolase 247 8e-64 >B1DA09.1 B1DA09_9BACL Metallophosphoesterase Length = 302 Score = 367 bits (943), Expect = e-100, Method: Compositional matrix adjust. Identities = 177/298 (59%), Positives = 232/298 (77%), Gaps = 3/298 (1%) Query: 1 MNKQRLSFHADGTFKIVQFTDIHLKDGIEPERDTRTLALMERILISEQPDLVVFSGDLVY 60 M KQ L+F DGTFKI+QFTDIH+ DG+ E D R+LAL++ ++ SE+PDL+VF+GDL++ Sbjct: 1 MGKQ-LAFRQDGTFKIIQFTDIHVYDGL-GEADVRSLALIKNLIESEKPDLIVFTGDLIF 58 Query: 61 SE-ETVDPKATFRRVIEVAVQAGTPFAVIYGNHDTEKGVTREELQAILTEYELCMAEAGP 119 ++ ET D + FR+ +++A Q+G PFAVIYGNHD E+ V + ELQ IL+E+ C+++AGP Sbjct: 59 ADNETGDLRGGFRKTVQIADQSGIPFAVIYGNHDAERNVKKPELQEILSEFGNCISDAGP 118 Query: 120 GEIHGTGNYVLPLFNHSADRESAALYFVDSGEYAPAAIGGYAWIHSDQVAWYLQESAKNR 179 +I G GNY + + S+D ++A LYF+DSGEYA +IGGYAWI QV WY ++S + Sbjct: 119 EDIGGIGNYTATVKSSSSDSDAAVLYFMDSGEYAHESIGGYAWIQPGQVQWYREQSRQLA 178 Query: 180 ERNGGVLPALAFMHIPIPEYQEVWQSGSVAGRKGEMVCCSKVNSGWFAAMLEAGDVMATF 239 ++N VLP LAF+HIPIPEY +VWQSG G KGE VCCSKVNSG FAA+LE GDVM F Sbjct: 179 DKNNAVLPGLAFLHIPIPEYNDVWQSGGAEGTKGEQVCCSKVNSGLFAALLERGDVMGVF 238 Query: 240 AGHDHDNDYIGVLHGITLAYGRVTGHNTYGSLQRGARVIQLKEGERRFETWIRQEDGS 297 AGHDH+NDYIG HGITLAYGR TG+NTYG L+RGAR+I L EGERRF+T+I++++ S Sbjct: 239 AGHDHNNDYIGKHHGITLAYGRATGYNTYGDLKRGARIITLVEGERRFDTYIKEDEQS 296 >B3K862.1 B3K862_9BACI Metallophosphoesterase Length = 319 Score = 299 bits (765), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 160/308 (51%), Positives = 197/308 (63%), Gaps = 13/308 (4%) Query: 3 KQRLSFHADGTFKIVQFTDIHLKDGIEPERDTRTLALMERILISEQPDLVVFSGDLVYSE 62 K L F D TFKIVQFTDIH K+G EPE D + ME +L EQPDLVVF+GDL+YS Sbjct: 2 KPILRFGDDHTFKIVQFTDIHWKNG-EPE-DLMSRRCMETVLDLEQPDLVVFTGDLIYSG 59 Query: 63 E-------TVDPKATFRRVIEVAVQAGTPFAVIYGNHDTEKGVTREELQAILTEYELCMA 115 E DP F+ + G +A ++GNHDTE +TREEL + ++ A Sbjct: 60 EADTGYRKCQDPAQAFKDAVSAVESRGIRWAFVFGNHDTEGEITREELMDVAMQHAYNCA 119 Query: 116 EAGPGEIHGTGNYVLPLFNHSADRESAALYFVDSG-EYAPAAIGGYAWIHSDQVAWY-LQ 173 E G +IHG GNY LPL+ + + +A LYF DSG E A+ GY WI DQ+ WY + Sbjct: 120 EHGSPDIHGVGNYTLPLYGSNGEETAAVLYFFDSGRESEHPAVPGYDWIRRDQIQWYEMA 179 Query: 174 ESAKNRERNGGVLPALAFMHIPIPEYQEVWQSGSVAGRKGEMVCCSKVNSGWFAAMLEAG 233 A + + G LP+LAF HIP+PEY+EVW+ + G K E VCC +VN+G FAAMLE G Sbjct: 180 SRAYSAKHQGNPLPSLAFFHIPLPEYREVWERRTCYGSKFERVCCPEVNTGLFAAMLERG 239 Query: 234 DVMATFAGHDHDNDYIGVLHGITLAYGRVTGHNTYG--SLQRGARVIQLKEGERRFETWI 291 DVM TFAGHDH NDY G LHGI L YGR TG+ TYG + RGARVI+L EG+R+F+TWI Sbjct: 240 DVMGTFAGHDHINDYWGELHGIRLCYGRATGYGTYGREGMLRGARVIRLHEGQRQFDTWI 299 Query: 292 RQEDGSVI 299 DGS I Sbjct: 300 TLSDGSQI 307 >B3KD47.1 B3KD47_9BACI Metallophosphoesterase Length = 320 Score = 276 bits (707), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 139/306 (45%), Positives = 196/306 (64%), Gaps = 14/306 (4%) Query: 3 KQRLSFHADGTFKIVQFTDIHLKDGIEP-ERDTRTLALMERILISEQPDLVVFSGDLVYS 61 ++ L F ++GTFKIVQFTD EP E + + A+M+RIL EQPDLV+++GD++ S Sbjct: 2 RKSLHFKSNGTFKIVQFTDTEF---CEPDEEELQMKAMMKRILEKEQPDLVIYTGDVIAS 58 Query: 62 EETVDPKATFRRVIEVAVQAGTPFAVIYGNHDTEKG-VTREELQAILTEYELCMAEAGPG 120 ++ +P F+ + V + P+A ++GNHD+E +TRE+L + + C A+ P Sbjct: 59 NKSPNPVQAFKDAVSVPEEMHIPWAAVFGNHDSEAAEMTREQLHYLQLSHRYCYAQPDPP 118 Query: 121 EIHGTGNYVLPLFNHSADRESAALYFVDSGEYAP---AAIGGYAWIHSDQVAWYLQESAK 177 +HG GNYVL + + + +AAL+F+DSG Y+P +G Y WI Q+AWY + S + Sbjct: 119 NVHGVGNYVLEILD-RLHQPAAALFFLDSGSYSPLEHMRVGLYDWIRRSQIAWYTEASHR 177 Query: 178 NRERNGG-VLPALAFMHIPIPEYQEVWQSGSVAGRKGEMVCCSK-VNSGWFAAMLEAGDV 235 RNGG LP+L F HIP+PEY ++W G+K + CC+ +N+G+FAAMLE GD+ Sbjct: 178 LTARNGGEPLPSLGFFHIPLPEYNDIWDFSVCYGQKLDAYCCAPLINTGFFAAMLEMGDI 237 Query: 236 MATFAGHDHDNDYIGVLHGITLAYGRVTGHNTY--GSLQRGARVIQLKEGERRFETWIRQ 293 M TF GHDH ND+ G LHGI L YGR T N Y Q GARVIQL EG+R FETW+ Sbjct: 238 MGTFVGHDHGNDFWGTLHGIRLCYGR-TSRNAYLDRPFQPGARVIQLTEGQRSFETWLHL 296 Query: 294 EDGSVI 299 EDG+++ Sbjct: 297 EDGTIV 302 >A6LGC4.1 A6LGC4_PARD8 Putative Icc family phosphohydrolase Length = 481 Score = 265 bits (677), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 132/298 (44%), Positives = 198/298 (66%), Gaps = 11/298 (3%) Query: 3 KQRLSFHADGTFKIVQFTDIHLKDGIEPERDTRTLALMERILISEQPDLVVFSGDLVYSE 62 +++L+F DG FKIVQFTDIH + + +T+A ++ +L +E PD+ + +GD+V Sbjct: 23 QKQLAFK-DGKFKIVQFTDIHWDQ--KSSKCAKTVATIQSVLKAENPDVAMLTGDVV--- 76 Query: 63 ETVDPKAT-FRRVIEVAVQAGTPFAVIYGNHDTEKGVTREELQAILTEYELCMAEAGPGE 121 T +P ++ VI + +A PF V+ GNHD E V ++E+ AIL++ M E GPG+ Sbjct: 77 -TANPGLEGWKSVIGIFEEAKIPFTVMMGNHDAEI-VPKDEIYAILSQSPYFMGEKGPGD 134 Query: 122 IHGTGNYVLPLFNHSADRESAALYFVDSGEYAPAA-IGGYAWIHSDQVAWYLQESAKNRE 180 IHG GNYV+P+++ + +A LY +DS +Y G Y WIH DQ+ WY ++S + + Sbjct: 135 IHGAGNYVVPVYSSDGKKPAALLYCIDSNDYPTLKDYGTYDWIHFDQINWYREQSMRYTK 194 Query: 181 RNGG-VLPALAFMHIPIPEYQEVWQSGSVAGRKGEMVCCSKVNSGWFAAMLEAGDVMATF 239 NGG LPALAF HIP+ EY E+ + + G+K E + K+N+G+FA+++E DVMATF Sbjct: 195 ENGGKPLPALAFFHIPLLEYNEIVGAETTLGQKEEGIASPKINTGFFASLVEMKDVMATF 254 Query: 240 AGHDHDNDYIGVLHGITLAYGRVTGHNTYGSLQRGARVIQLKEGERRFETWIRQEDGS 297 AGHDHDNDYIG+L+ + LA+GRV+G + YG +RG R+I+L+EG+ F++WIR G Sbjct: 255 AGHDHDNDYIGMLYNVGLAFGRVSGWDAYGDFERGGRIIELREGKFEFDSWIRTSSGK 312 >C3QH80.1 C3QH80_9BACE Icc family phosphohydrolase Length = 335 Score = 247 bits (631), Expect = 8e-64, Method: Compositional matrix adjust. Identities = 142/308 (46%), Positives = 187/308 (60%), Gaps = 25/308 (8%) Query: 3 KQRLSFHADGTFKIVQFTDIHLKDGIEPERDTRTLALMERILISEQPDLVVFSGDLVYSE 62 K L F DG FKIVQFTD+H K G L + ++L E+PDLV+F+GD+VYS Sbjct: 24 KSELKFSKDGKFKIVQFTDVHFKYGNRAS--DIALERINQVLDDERPDLVIFTGDVVYSA 81 Query: 63 ETVDPKATFRRVIEVAVQAGTPFAVIYGNHDTEKGVTREELQAILTEYELCMAEAGPGEI 122 + +V+E V+ PF V +GNHD E+G+TRE+L I+ + PG + Sbjct: 82 PA---DSGMLQVLEPVVKRKLPFVVTFGNHDNEQGMTREQLYDIIRKV--------PGNL 130 Query: 123 ---HGT---GNYVLPLFNHS-ADRESAALYFVDSGEYAPAA-IGGYAWIHSDQVAWYLQE 174 GT +YVL + + S +++A LY +DS Y+P + GYAW+ DQ+ WY Q+ Sbjct: 131 LPDRGTVLSPDYVLTVKSSSNVKKDAALLYCMDSHSYSPLKDVKGYAWLTFDQINWYRQQ 190 Query: 175 SAKNRERNGG-VLPALAFMHIPIPEYQEVWQSGS--VAGRKGEMVCCSKVNSGWFAAMLE 231 SA + +NGG LPALAF HIP+PEY E ++ + + G + E C K+N+G FAAM E Sbjct: 191 SAAYKAQNGGQPLPALAFFHIPLPEYNEAARTENAILRGTRMEEACAPKLNTGMFAAMKE 250 Query: 232 AGDVMATFAGHDHDNDYIGVLHGITLAYGRVTGHNT-YGSLQRGARVIQLKEGERRFETW 290 AGDVM F GHDHDNDY + GI LAYGR TG NT Y L GAR+I L EG R F +W Sbjct: 251 AGDVMGMFVGHDHDNDYAVMWKGILLAYGRFTGGNTEYNHLPNGARIIVLDEGARTFTSW 310 Query: 291 IRQEDGSV 298 IRQ+DG V Sbjct: 311 IRQKDGVV 318 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,903,201,310 Number of extensions: 82183097 Number of successful extensions: 188465 Number of sequences better than 1.0e-05: 195 Number of HSP's gapped: 187722 Number of HSP's successfully gapped: 199 Length of query: 299 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 164 Effective length of database: 1,788,859,178 Effective search space: 293372905192 Effective search space used: 293372905192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)