BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0268.0003.p (243 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KA03.1 B3KA03_9BACI Nucleotidyl transferase 293 1e-77 B1D798.1 B1D798_9BACL Nucleotidyl transferase 248 3e-64 B5YBG5.1 B5YBG5_DICT6 Glucose-1-phosphate thymidylyltransferase ... 246 1e-63 C3B0M0.1 C3B0M0_BACMY Spore coat polysaccharide biosynthesis pro... 243 2e-62 C3AVT1.1 C3AVT1_BACMY Spore coat polysaccharide biosynthesis pro... 241 3e-62 >B3KA03.1 B3KA03_9BACI Nucleotidyl transferase Length = 235 Score = 293 bits (749), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 142/238 (59%), Positives = 177/238 (74%), Gaps = 4/238 (1%) Query: 1 VKGVILAGGTGSRLYPLTRLMNKHLLPVGKYPMVCYGIDRLRRGGITDILLVISKQSAGQ 60 +KGVILAGGTG+RLYPLT+++NKHLLPVGK PM+C+GI++ ++ GI +IL+VI KQSAG Sbjct: 1 MKGVILAGGTGTRLYPLTKVINKHLLPVGKVPMICHGIEKFKKAGIEEILIVIGKQSAGL 60 Query: 61 YTDFLGSGAEFGVSLTYXXXXXXXXXXXXXXXXXXFILPGERFVVLLGDNLFMDDLEPYV 120 YTD +GSG E+GVS+T+ FI PGE+FVVLLGDNLF D L +V Sbjct: 61 YTDLIGSGKEWGVSVTFKIQEEAGGIAQALYLAEPFITPGEKFVVLLGDNLFEDHLSTFV 120 Query: 121 KKYLQQPPGTAKVLLKPVEDARRYGVPVFDSQDTGLIAYIEEKPEHPKTKYCVTGIYMYD 180 + Y +Q G A+VLLK V D +RYGVPV + + I IEEKPE PK+ YCVTGIYMYD Sbjct: 121 QAYEKQAAG-ARVLLKKVLDPKRYGVPVLEKDN---IVMIEEKPEDPKSDYCVTGIYMYD 176 Query: 181 EAVFDIIRRVSPSQRGELEITDVNNIYASNRRLSYDVLKEWWSDAGTFQSLYEAGEKL 238 VF+ IR + PS RGELEITDVNN+YAS +L+YD+L WW+DAGTFQSLY+A +L Sbjct: 177 STVFETIRTIKPSARGELEITDVNNVYASKGQLTYDILDGWWTDAGTFQSLYQASGRL 234 >B1D798.1 B1D798_9BACL Nucleotidyl transferase Length = 240 Score = 248 bits (634), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 122/237 (51%), Positives = 162/237 (68%), Gaps = 3/237 (1%) Query: 1 VKGVILAGGTGSRLYPLTRLMNKHLLPVGKYPMVCYGIDRLRRGGITDILLVISKQSAGQ 60 +K VILAGGTG+RL PLT +NKH+LPVG++PM+ +GI+ LR+ GI +IL++ ++QSAG Sbjct: 1 MKAVILAGGTGTRLLPLTSYINKHMLPVGRHPMIHHGIETLRKAGICEILIITNRQSAGM 60 Query: 61 YTDFLGSGAEFGVSLTYXXXXXXXXXXXXXXXXXXFILPGERFVVLLGDNLFMDDLEPYV 120 + + GSG + VSL Y FI GE+F+VLLGDNLF +DL+PY+ Sbjct: 61 FIQYFGSGEDQAVSLVYRIQEGAGGIADALQLARPFIADGEKFLVLLGDNLFEEDLKPYI 120 Query: 121 KKYLQQPPGTAKVLLKPVEDARRYGVPVFDSQDTGLIAYIEEKPEHPKTKYCVTGIYMYD 180 + QQ G A VLLK V+D RYG+PVFDS +G I +IEEKP P++ VTG+YMY Sbjct: 121 HDFRQQQDG-AMVLLKEVDDPHRYGIPVFDS--SGAIKHIEEKPAEPQSNLSVTGLYMYS 177 Query: 181 EAVFDIIRRVSPSQRGELEITDVNNIYASNRRLSYDVLKEWWSDAGTFQSLYEAGEK 237 VF+ I +SPS RGELEITD+NN+YA+ L+Y + WW DAGTF+SL EA + Sbjct: 178 SDVFERIENISPSSRGELEITDLNNLYAAAGLLTYRTISGWWMDAGTFESLKEASAR 234 >B5YBG5.1 B5YBG5_DICT6 Glucose-1-phosphate thymidylyltransferase EC=2.7.7.24 Length = 246 Score = 246 bits (629), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 127/242 (52%), Positives = 167/242 (69%), Gaps = 5/242 (2%) Query: 1 VKGVILAGGTGSRLYPLTRLMNKHLLPVGKYPMVCYGIDRLRRGGITDILLVISKQSAGQ 60 VKGVILAGGTGSRL PLT++ NKHLLPVG+YPM+ Y I +L++ GI +IL+V ++ G Sbjct: 6 VKGVILAGGTGSRLRPLTKVTNKHLLPVGRYPMIFYPIYKLKQAGIYEILVVTGREHMGD 65 Query: 61 YTDFLGSGAEFGVSLTYXXXXXXXXXXXXXXXXXXFILPGERFVVLLGDNLFMDDLEPYV 120 + LGSG +FGV TY F+ ++ VV+LGDN+F DD+ PYV Sbjct: 66 VIELLGSGRDFGVEFTYKVQDEAGGIAQALGLARHFV-GDDKCVVILGDNIFEDDITPYV 124 Query: 121 KKYLQQPPGTAKVLLKPVEDARRYGVPVFDSQDTGLIAYIEEKPEHPKTKYCVTGIYMYD 180 KK+ +Q G AK+LLK V D +R+GVPVF++ G I IEEKP+ PK+ + VTGIYMYD Sbjct: 125 KKFEEQKEG-AKILLKEVPDPQRFGVPVFEN---GRIIAIEEKPKEPKSNFAVTGIYMYD 180 Query: 181 EAVFDIIRRVSPSQRGELEITDVNNIYASNRRLSYDVLKEWWSDAGTFQSLYEAGEKLKN 240 VF+II+ + PS RGELEITDVNN Y +L+YD+LK WW+DAGT +SL+ A E +N Sbjct: 181 SYVFEIIKTLKPSARGELEITDVNNTYLKMGKLTYDILKGWWTDAGTPESLFRANELARN 240 Query: 241 TL 242 + Sbjct: 241 II 242 >C3B0M0.1 C3B0M0_BACMY Spore coat polysaccharide biosynthesis protein spsI Length = 249 Score = 243 bits (619), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 121/234 (51%), Positives = 159/234 (67%), Gaps = 5/234 (2%) Query: 1 VKGVILAGGTGSRLYPLTRLMNKHLLPVGKYPMVCYGIDRLRRGGITDILLVISKQSAGQ 60 +KG+ILAGGTGSRLYP+T++ NKHLLPVG+YPM+ + + +LR GITDI+++ K+ G Sbjct: 1 MKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHSVYKLRECGITDIIVITGKEHMGD 60 Query: 61 YTDFLGSGAEFGVSLTYXXXXXXXXXXXXXXXXXXFILPGERFVVLLGDNLFMDDLEPYV 120 FLGSGA+FGVS TY F+ G + +V+LGDN+F D L+PYV Sbjct: 61 VVSFLGSGADFGVSFTYRVQEKAGGIAEALGLCETFVGNG-KMIVILGDNVFSDSLKPYV 119 Query: 121 KKYLQQPPGTAKVLLKPVEDARRYGVPVFDSQDTGLIAYIEEKPEHPKTKYCVTGIYMYD 180 + Y +Q G A++LLK V+D RR+GVP G I IEEKP PK+ Y V GIYMYD Sbjct: 120 ENYRKQGAG-ARILLKEVDDPRRFGVPHIQE---GKIVCIEEKPASPKSNYAVIGIYMYD 175 Query: 181 EAVFDIIRRVSPSQRGELEITDVNNIYASNRRLSYDVLKEWWSDAGTFQSLYEA 234 +VF +I ++ PS RGELE+TD+NN Y + L+YD LK WW+DAGT SLY+A Sbjct: 176 NSVFSMISQLKPSDRGELEVTDINNEYLARGVLTYDTLKGWWTDAGTHPSLYKA 229 >C3AVT1.1 C3AVT1_BACMY Spore coat polysaccharide biosynthesis protein spsI Length = 249 Score = 241 bits (616), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 122/234 (52%), Positives = 161/234 (68%), Gaps = 5/234 (2%) Query: 1 VKGVILAGGTGSRLYPLTRLMNKHLLPVGKYPMVCYGIDRLRRGGITDILLVISKQSAGQ 60 +KG+ILAGGTGSRLYP+T++ NKHLLPVG+YPM+ + + +LR GITDI+++ K+ G Sbjct: 1 MKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHSVYKLRECGITDIIVITGKEHMGD 60 Query: 61 YTDFLGSGAEFGVSLTYXXXXXXXXXXXXXXXXXXFILPGERFVVLLGDNLFMDDLEPYV 120 FLGSGA+FGV+ TY F+ G + +V+LGDN+F D L+PYV Sbjct: 61 VVSFLGSGADFGVAFTYRVQEKAGGIAEALGLCETFVGNG-KMIVVLGDNVFSDSLKPYV 119 Query: 121 KKYLQQPPGTAKVLLKPVEDARRYGVPVFDSQDTGLIAYIEEKPEHPKTKYCVTGIYMYD 180 + Y +Q G A++LLK V+D RR+GVP QD G I IEEKP PK+ Y V GIYMYD Sbjct: 120 ENYRKQGAG-ARILLKEVDDPRRFGVPHI--QD-GKIVCIEEKPASPKSNYAVIGIYMYD 175 Query: 181 EAVFDIIRRVSPSQRGELEITDVNNIYASNRRLSYDVLKEWWSDAGTFQSLYEA 234 +VF +I ++ PS RGELE+TD+NN Y + L+YD LK WW+DAGT SLY+A Sbjct: 176 NSVFSMISQLKPSDRGELEVTDINNEYLARGVLTYDTLKGWWTDAGTHPSLYKA 229 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.141 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,445,360,902 Number of extensions: 59383236 Number of successful extensions: 154228 Number of sequences better than 1.0e-05: 2235 Number of HSP's gapped: 150858 Number of HSP's successfully gapped: 2235 Length of query: 243 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 111 Effective length of database: 1,817,122,223 Effective search space: 201700566753 Effective search space used: 201700566753 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)