BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0267.0018.p (427 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8W6.1 B3K8W6_9BACI Metal-dependent phosphohydrolase HD sub do... 650 0.0 B1DIJ4.1 B1DIJ4_9BACL Metal dependent phosphohydrolase 540 e-152 Q9K6B1.1 Q9K6B1_BACHD BH3818 protein 452 e-125 C3IZ91.1 C3IZ91_9BACI Metal dependent phosphohydrolase 451 e-125 B7Y7A0.1 B7Y7A0_9BACI Metal dependent phosphohydrolase 451 e-125 >B3K8W6.1 B3K8W6_9BACI Metal-dependent phosphohydrolase HD sub domain Length = 429 Score = 650 bits (1678), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/427 (73%), Positives = 354/427 (82%) Query: 1 MKQLLTEEKVFKDPVHNYIHVQDTMIWRLINTREFQRLRRIRQLGTSYLTFHGAEHSRFS 60 M ++EEKVFKDPVHNYIHVQD +IWRLI+T EFQRLRR+RQLGTS+LTFHGAEHSRFS Sbjct: 1 MHSPISEEKVFKDPVHNYIHVQDDIIWRLIDTPEFQRLRRVRQLGTSFLTFHGAEHSRFS 60 Query: 61 HSLGVYEITRRIISQFERSGYKDWMPEESXXXXXXXXXXXXGHGPFSHSIEEAFEMNHED 120 HSLGVYEITRRIISQFER GY DW EE GHGPFSHSIEEAF MNHED Sbjct: 61 HSLGVYEITRRIISQFERRGYSDWPAEERQVALCAALLHDVGHGPFSHSIEEAFHMNHED 120 Query: 121 WTCRIIQENTEITAILREAGEDFPQKVASVIAKTYEHEIVVNLVSGPLDADRMDYLLRDA 180 WTCRI+ +T + +L+E G DFP+KVASVI KTY+ IV+NLV+ PLDADRMDYLLRDA Sbjct: 121 WTCRILLGDTCVNRVLQELGADFPEKVASVINKTYDKPIVMNLVTSPLDADRMDYLLRDA 180 Query: 181 YYTGVNYGTIDIDRILRMLRPYNGRVVVKESGMHAIEDYLMSRYQMYWQVYFHPVTRSSE 240 YYTGVNYGTIDIDRILRMLRPY RVVVKESGMHA+EDYLMSRYQMYWQVYFHPVTRSSE Sbjct: 181 YYTGVNYGTIDIDRILRMLRPYRDRVVVKESGMHAVEDYLMSRYQMYWQVYFHPVTRSSE 240 Query: 241 IILRQIFRRAKELFQEGYSFRFMINPLSDLFGGEINVEQYLLLDEALVQTAFMQWTLEDD 300 IILRQIFRRAKEL GY+FRFMI PL LF G I V QYL LDEAL+QTAF+QWT E+D Sbjct: 241 IILRQIFRRAKELHDRGYAFRFMIEPLPALFSGNIGVHQYLRLDEALIQTAFLQWTEEED 300 Query: 301 EILANLCSRFIHRKLYKYVEMDHLDSETIDEIRRSFAGAGLHPDYDLEIDYPTDLPYDVF 360 +L+ LC RF+HRKLYKYVE+D++D ETID IR++F GLHP+YDLEID+PTDLPYD Sbjct: 301 PLLSELCGRFMHRKLYKYVEIDNIDLETIDGIRQAFRAVGLHPEYDLEIDFPTDLPYDKV 360 Query: 361 RPGEGFDSKQILLLDRHDKLREISEVSDIVRSISGIHRGRYHLYFPQDKLQKALTRLPVS 420 + KQILLLDRHD+LREISEVSDIVRSISGIH+GRYHLY+P++K+ L ++P + Sbjct: 361 HSDMPLNEKQILLLDRHDELREISEVSDIVRSISGIHKGRYHLYYPEEKVDPLLGQMPET 420 Query: 421 IGGIFAK 427 I +F + Sbjct: 421 IADVFKQ 427 >B1DIJ4.1 B1DIJ4_9BACL Metal dependent phosphohydrolase Length = 431 Score = 540 bits (1392), Expect = e-152, Method: Compositional matrix adjust. Identities = 263/429 (61%), Positives = 319/429 (74%), Gaps = 4/429 (0%) Query: 1 MKQLLTEEKVFKDPVHNYIHVQDTMIWRLINTREFQRLRRIRQLGTSYLTFHGAEHSRFS 60 M Q LTEEKVFKDPVH YI+VQD +W LINT+EFQRLRRIRQLGTSYLTFHGAEHSRFS Sbjct: 1 MNQQLTEEKVFKDPVHKYIYVQDQTVWDLINTKEFQRLRRIRQLGTSYLTFHGAEHSRFS 60 Query: 61 HSLGVYEITRRIISQFERSGYKDWMPEESXXXXXXXXXXXXGHGPFSHSIEEAFEMNHED 120 HSLGVYEITR+IISQFER+ Y W EE GHGPFSHS+E+ F+ +HE Sbjct: 61 HSLGVYEITRKIISQFERNEYPGWPMEERLLALCAALLHDVGHGPFSHSLEQVFDTHHEQ 120 Query: 121 WTCRIIQENTEITAILREAGEDFPQKVASVIAKTYEHEIVVNLVSGPLDADRMDYLLRDA 180 WTC I+ E+TE+ +LR EDFP+KVASVI+KTY + IVV+L+S +DADRMDYLLRDA Sbjct: 121 WTCAILLEDTEVNRVLRRVSEDFPEKVASVISKTYPNPIVVSLISSQMDADRMDYLLRDA 180 Query: 181 YYTGVNYGTIDIDRILRMLRPYNGRVVVKESGMHAIEDYLMSRYQMYWQVYFHPVTRSSE 240 Y+TGVNYG D++RILR+LRP G++VVK+SGMHAIE YLMSRYQMYWQ+YFHPVTRSSE Sbjct: 181 YFTGVNYGNFDLERILRVLRPVQGKIVVKDSGMHAIEHYLMSRYQMYWQIYFHPVTRSSE 240 Query: 241 IILRQIFRRAKELFQEGYSFRFMINPLSDLFGGEINVEQYLLLDEALVQTAFMQWTLEDD 300 IILRQI RRAKEL+ GY+F +M P++ L G I V YL LDEA +QT F WT E D Sbjct: 241 IILRQITRRAKELYDSGYAFSWMPGPINRLLDGNITVPDYLKLDEAYMQTIFSLWTEEKD 300 Query: 301 EILANLCSRFIHRKLYKYVEMDHLDSETIDEIRRSFAGAGLHPDYDLEIDYPTDLPYDVF 360 +LA LC RF++R+LYKY+ +D ++E F GL+PDY +EID+P D PYD++ Sbjct: 301 ALLAELCDRFLNRQLYKYITLDDPGDSLLEEAALEFIAIGLNPDYHMEIDFPFDQPYDIY 360 Query: 361 RP----GEGFDSKQILLLDRHDKLREISEVSDIVRSISGIHRGRYHLYFPQDKLQKALTR 416 RP G D ILLL + L EIS SDIVRSISG+H+G+YH+Y+P + L A R Sbjct: 361 RPDAKKGGKEDKAPILLLSDDNSLSEISVKSDIVRSISGLHQGQYHVYYPIEPLLAAADR 420 Query: 417 LPVSIGGIF 425 L ++ +F Sbjct: 421 LSPAVRKLF 429 >Q9K6B1.1 Q9K6B1_BACHD BH3818 protein Length = 432 Score = 452 bits (1162), Expect = e-125, Method: Compositional matrix adjust. Identities = 222/408 (54%), Positives = 297/408 (72%), Gaps = 3/408 (0%) Query: 4 LLTEEKVFKDPVHNYIHVQDTMIWRLINTREFQRLRRIRQLGTSYLTFHGAEHSRFSHSL 63 LL EEKVFKDPVH YIHV+D +IW LI T+EFQRLRR+RQLGT++LTFHGAEH+RF+HSL Sbjct: 8 LLDEEKVFKDPVHRYIHVRDELIWALIGTKEFQRLRRVRQLGTTFLTFHGAEHTRFNHSL 67 Query: 64 GVYEITRRIISQFERSGYKDWMPEESXXXXXXXXXXXXGHGPFSHSIEEAFEMNHEDWTC 123 GVYEITRRII F+ Y W EE GHGPFSHS E+ F+ +HE+WT Sbjct: 68 GVYEITRRIIEVFQGRPY--WNEEERLLTLCAALLHDIGHGPFSHSFEKVFDTDHEEWTR 125 Query: 124 RIIQENTEITAILREAGEDFPQKVASVIAKTYEHEIVVNLVSGPLDADRMDYLLRDAYYT 183 R+I +TEI +L + G+DFPQKVA VI KTY +++V +++S +DADRMDYL RDAYYT Sbjct: 126 RMIVGDTEIHNVLLKMGDDFPQKVADVIEKTYPNKLVTSIISSQIDADRMDYLQRDAYYT 185 Query: 184 GVNYGTIDIDRILRMLRPYNGRVVVKESGMHAIEDYLMSRYQMYWQVYFHPVTRSSEIIL 243 GV+YG D++RILR++RP +VV+K+SGMHA+EDY+MSRYQMYWQVYFHPVTRS+E+IL Sbjct: 186 GVSYGHFDMERILRVMRPMEDQVVIKQSGMHAVEDYIMSRYQMYWQVYFHPVTRSAEVIL 245 Query: 244 RQIFRRAKELFQEGYSFRFMINPLSDLFGGEINVEQYLLLDEALVQTAFMQWTLEDDEIL 303 ++F+R K+L+++GY F+ LF G ++++ YL LDE++ F W E+D IL Sbjct: 246 SKVFKRVKDLYEQGYKFKQEPKHFYSLFEGNMSLDDYLRLDESITMYYFQIWQEEEDRIL 305 Query: 304 ANLCSRFIHRKLYKYVEMD-HLDSETIDEIRRSFAGAGLHPDYDLEIDYPTDLPYDVFRP 362 ++LC RFI+R+L+KY+E + +L +++ FA A + P+Y L +D +DLPYD +RP Sbjct: 306 SDLCVRFINRQLFKYIEFNPNLQMNDWPRLQQLFAQAEIDPEYYLVVDSSSDLPYDFYRP 365 Query: 363 GEGFDSKQILLLDRHDKLREISEVSDIVRSISGIHRGRYHLYFPQDKL 410 GE + I L+ + KLRE+S SD+V +ISG R + LYFP D L Sbjct: 366 GEEEERLPIHLIMPNGKLRELSRESDVVEAISGKKRTDHKLYFPMDCL 413 >C3IZ91.1 C3IZ91_9BACI Metal dependent phosphohydrolase Length = 432 Score = 451 bits (1161), Expect = e-125, Method: Compositional matrix adjust. Identities = 231/418 (55%), Positives = 292/418 (69%), Gaps = 3/418 (0%) Query: 5 LTEEKVFKDPVHNYIHVQDTMIWRLINTREFQRLRRIRQLGTSYLTFHGAEHSRFSHSLG 64 L EEKVFKDPVH YIHV+D +IW LI T+EFQRLRRI+QLGT+YLTFHGAEHSRF+HSLG Sbjct: 7 LDEEKVFKDPVHRYIHVRDKVIWDLIGTKEFQRLRRIKQLGTTYLTFHGAEHSRFNHSLG 66 Query: 65 VYEITRRIISQFERSGYKDWMPEESXXXXXXXXXXXXGHGPFSHSIEEAFEMNHEDWTCR 124 VYEI RRII G W E GHGPFSHS E+ F ++HED+T Sbjct: 67 VYEIIRRIIDDV-FVGRDHWDHSERLLCLCAALLHDLGHGPFSHSFEKVFRLDHEDFTQA 125 Query: 125 IIQENTEITAILREAGEDFPQKVASVIAKTYEHEIVVNLVSGPLDADRMDYLLRDAYYTG 184 II +TE+ A LRE G+DFPQKVA VIAKTY +++VV+L+S +DADRMDYLLRDAYYTG Sbjct: 126 IILGDTEVNAALREVGDDFPQKVAEVIAKTYPNKLVVSLISSQIDADRMDYLLRDAYYTG 185 Query: 185 VNYGTIDIDRILRMLRPYNGRVVVKESGMHAIEDYLMSRYQMYWQVYFHPVTRSSEIILR 244 V+YG D++RILR++RP +VV+K SGMHA+EDY+MSRYQMYWQVYFHPVTRS+E+IL Sbjct: 186 VSYGYFDMERILRVMRPREDQVVIKRSGMHAVEDYIMSRYQMYWQVYFHPVTRSAEVILT 245 Query: 245 QIFRRAKELFQEGYSFRFMINPLSDLFGGEINVEQYLLLDEALVQTAFMQWTLEDDEILA 304 +I RAK+L++EGY F+ F G +++ YL LDEA++ F QW E D IL+ Sbjct: 246 KILHRAKKLYEEGYRFQTKPVHFLSFFAGNVSLGDYLALDEAVILFYFQQWQYERDPILS 305 Query: 305 NLCSRFIHRKLYKYVEMDHLDS--ETIDEIRRSFAGAGLHPDYDLEIDYPTDLPYDVFRP 362 +LC RF+ R+L+KY E + E + E+ F AG+ PDY L +D +DLPYD +RP Sbjct: 306 DLCQRFVRRRLFKYTEFHPTNEQMEKLIELTGLFKKAGIDPDYYLVVDSSSDLPYDFYRP 365 Query: 363 GEGFDSKQILLLDRHDKLREISEVSDIVRSISGIHRGRYHLYFPQDKLQKALTRLPVS 420 GE + I LL + +LRE+S S +V +ISG R + LYFP D L+ T+ V Sbjct: 366 GEEGERLPIYLLMPNGELRELSRESVLVDAISGKRRTDHKLYFPADFLEDLSTKRTVK 423 >B7Y7A0.1 B7Y7A0_9BACI Metal dependent phosphohydrolase Length = 432 Score = 451 bits (1161), Expect = e-125, Method: Compositional matrix adjust. Identities = 231/418 (55%), Positives = 292/418 (69%), Gaps = 3/418 (0%) Query: 5 LTEEKVFKDPVHNYIHVQDTMIWRLINTREFQRLRRIRQLGTSYLTFHGAEHSRFSHSLG 64 L EEKVFKDPVH YIHV+D +IW LI T+EFQRLRRI+QLGT+YLTFHGAEHSRF+HSLG Sbjct: 7 LDEEKVFKDPVHRYIHVRDKVIWDLIGTKEFQRLRRIKQLGTTYLTFHGAEHSRFNHSLG 66 Query: 65 VYEITRRIISQFERSGYKDWMPEESXXXXXXXXXXXXGHGPFSHSIEEAFEMNHEDWTCR 124 VYEI RRII G W E GHGPFSHS E+ F ++HED+T Sbjct: 67 VYEIIRRIIDDV-FVGRDHWDHSERLLCLCAALLHDLGHGPFSHSFEKVFRLDHEDFTQA 125 Query: 125 IIQENTEITAILREAGEDFPQKVASVIAKTYEHEIVVNLVSGPLDADRMDYLLRDAYYTG 184 II +TE+ A LRE G+DFPQKVA VIAKTY +++VV+L+S +DADRMDYLLRDAYYTG Sbjct: 126 IILGDTEVNAALREVGDDFPQKVAEVIAKTYPNKLVVSLISSQIDADRMDYLLRDAYYTG 185 Query: 185 VNYGTIDIDRILRMLRPYNGRVVVKESGMHAIEDYLMSRYQMYWQVYFHPVTRSSEIILR 244 V+YG D++RILR++RP +VV+K SGMHA+EDY+MSRYQMYWQVYFHPVTRS+E+IL Sbjct: 186 VSYGYFDMERILRVMRPREDQVVIKRSGMHAVEDYIMSRYQMYWQVYFHPVTRSAEVILT 245 Query: 245 QIFRRAKELFQEGYSFRFMINPLSDLFGGEINVEQYLLLDEALVQTAFMQWTLEDDEILA 304 +I RAK+L++EGY F+ F G +++ YL LDEA++ F QW E D IL+ Sbjct: 246 KILHRAKKLYEEGYRFQTKPVHFLSFFAGNVSLGDYLALDEAVILFYFQQWQYERDPILS 305 Query: 305 NLCSRFIHRKLYKYVEMDHLDS--ETIDEIRRSFAGAGLHPDYDLEIDYPTDLPYDVFRP 362 +LC RF+ R+L+KY E + E + E+ F AG+ PDY L +D +DLPYD +RP Sbjct: 306 DLCQRFVRRRLFKYTEFHPTNEQMEKLIELTGLFKKAGIDPDYYLVVDSSSDLPYDFYRP 365 Query: 363 GEGFDSKQILLLDRHDKLREISEVSDIVRSISGIHRGRYHLYFPQDKLQKALTRLPVS 420 GE + I LL + +LRE+S S +V +ISG R + LYFP D L+ T+ V Sbjct: 366 GEEGERLPIYLLMPNGELRELSRESVLVDAISGKRRTDHKLYFPADFLEDLSTKRTVK 423 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.140 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,514,938,520 Number of extensions: 103970469 Number of successful extensions: 235450 Number of sequences better than 1.0e-05: 729 Number of HSP's gapped: 233781 Number of HSP's successfully gapped: 742 Length of query: 427 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 288 Effective length of database: 1,751,175,118 Effective search space: 504338433984 Effective search space used: 504338433984 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)