BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0267.0006.p (289 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8V4.1 B3K8V4_9BACI Orn/Lys/Arg decarboxylase major region 327 7e-88 B1DIK4.1 B1DIK4_9BACL Orn/Lys/Arg decarboxylase major region 199 3e-49 C0ZHB0.1 C0ZHB0_BREBN Putative uncharacterized protein yaaO 172 3e-41 Q5L3X0.1 Q5L3X0_GEOKA Lysine decarboxylase 141 7e-32 C3J4M2.1 C3J4M2_9BACI Orn/Lys/Arg decarboxylase major region 141 9e-32 >B3K8V4.1 B3K8V4_9BACI Orn/Lys/Arg decarboxylase major region Length = 511 Score = 327 bits (838), Expect = 7e-88, Method: Compositional matrix adjust. Identities = 174/276 (63%), Positives = 190/276 (68%), Gaps = 17/276 (6%) Query: 1 MLAGVRAVFIEPQIDPLSGLXXXXXXXXXXXXXXXXXXXXXXXXTMPNYYGMGSDLAPLA 60 MLAG RAVF++P++D SGL TMPNYYGMGSDL PLA Sbjct: 83 MLAGARAVFLQPELDKQSGLAVAPSEATLRAALEAFPEAKGAIITMPNYYGMGSDLRPLA 142 Query: 61 RACHTSGVPLLVDEAHGAHYGQHPALPAGALMCGADGVVQSTHKMLPALTMGAMLHVQGP 120 ACH GVPLLVDEAHGAHYGQHP LP AL CGADGVVQSTHKML A+TMGAMLHVQG Sbjct: 143 EACHKRGVPLLVDEAHGAHYGQHPQLPMNALSCGADGVVQSTHKMLAAMTMGAMLHVQGG 202 Query: 121 WLDRTLLRQRLAMVQSSSPSYPVMASLDLARRLVHSQGAGAFTAGLAAVDVLRRGLATLP 180 LDR+LLRQRLAMVQSSSPSYP+MASLDLARRL+H+QGAGAFTAGLAAVD RRGLA LP Sbjct: 203 LLDRSLLRQRLAMVQSSSPSYPLMASLDLARRLLHTQGAGAFTAGLAAVDAFRRGLAKLP 262 Query: 181 RYGLLQPAVPLQGACXXXXXXXXXXXAAASTPPQGMAAYSTQDPFKAVIYDAAGVLGGFE 240 R+GLLQP P AAY+ QDPFKAVIYD G L G+ Sbjct: 263 RFGLLQPPQP-----------------EDGAAGTAAAAYTRQDPFKAVIYDRTGALSGYA 305 Query: 241 LQRRLEEKGMVPEMSDDRHVVLAFSLGSKAEDTQRL 276 LQR LE +G VPEMSD+ VVL F+LGS ED + Sbjct: 306 LQRALEARGCVPEMSDELRVVLLFTLGSTREDANHV 341 >B1DIK4.1 B1DIK4_9BACL Orn/Lys/Arg decarboxylase major region Length = 490 Score = 199 bits (505), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 114/275 (41%), Positives = 149/275 (54%), Gaps = 26/275 (9%) Query: 2 LAGVRAVFIEPQIDPLSGLXXXXXXXXXXXXXXXXXXXXXXXXTMPNYYGMGSDLAPLAR 61 LAGVRA+F+ P++D +GL T PNYYGM ++L A Sbjct: 126 LAGVRAIFVTPRMDEETGLPTTPSLECIEQALELYPEAKAVFLTNPNYYGMTTNLTSYAE 185 Query: 62 ACHTSGVPLLVDEAHGAHYGQHPALPAGALMCGADGVVQSTHKMLPALTMGAMLHVQGPW 121 HT LLVDEAHGAHYG +PA P A+ GAD VVQSTHK L ALTMGAMLH++G Sbjct: 186 LVHTYNSLLLVDEAHGAHYGLNPAFPESAIQAGADAVVQSTHKTLSALTMGAMLHLKGDR 245 Query: 122 LDRTLLRQRLAMVQSSSPSYPVMASLDLARRLVHSQGAGAFTAGLAAVDVLRRGLATLPR 181 + R +++ LA +QSSSPS+P+MASLD+AR +V GA F G+A+V R + Sbjct: 246 IPRASIKEVLAAIQSSSPSFPIMASLDVARVMVDRYGAEWFELGVASVQAFRTWI----- 300 Query: 182 YGLLQPAVPLQGACXXXXXXXXXXXAAASTPPQGMAAYSTQDPFKAVIYDAAGVLGGFEL 241 V GA QG AY + DP + V++D G GFEL Sbjct: 301 -------VKEDGALKVLQAD------------QGSTAYDSLDPLRVVLWDRTGTFTGFEL 341 Query: 242 QRRLEEKGMVPEMSDDRHVVLAFSLGSKAEDTQRL 276 Q+ +EE+G EM+D +HVVL + G + + RL Sbjct: 342 QKLMEEQGCWAEMADIKHVVLVW--GCRTGEDARL 374 >C0ZHB0.1 C0ZHB0_BREBN Putative uncharacterized protein yaaO Length = 490 Score = 172 bits (437), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 105/276 (38%), Positives = 140/276 (50%), Gaps = 26/276 (9%) Query: 1 MLAGVRAVFIEPQIDPLSGLXXXXXXXXXXXXXXXXXXXXXXXXTMPNYYGMGSDLAPLA 60 M+A +F+ P +D +G+ T P YYGMG DLA LA Sbjct: 131 MMARATPIFLVPAVDLATGVAAGVRREDVERALLAHPDAKAVFLTNPTYYGMGIDLAKLA 190 Query: 61 RACHTSGVPLLVDEAHGAHYGQHPALPAGALMCGADGVVQSTHKMLPALTMGAMLHVQGP 120 H VPLL+DEAHGAHYG HPA P A+ GAD VQSTHKM A+TM +MLH+QG Sbjct: 191 ATVHRFDVPLLIDEAHGAHYGFHPAFPRSAMQEGADASVQSTHKMGTAMTMSSMLHIQGE 250 Query: 121 WLDRTLLRQRLAMVQSSSPSYPVMASLDLARRLVHSQGAGAFTAGLAAVDVLRRGLATLP 180 +DR L + LAM+QSSSPSY +MASLD+ARR + + + L +D R L L Sbjct: 251 RIDRERLFRHLAMLQSSSPSYVLMASLDVARRHLVLEATEEWNRLLPLLDKFRERLDRLD 310 Query: 181 RYGLLQPAVPLQGACXXXXXXXXXXXAAASTPPQGMAAYSTQDPFKAVIYDAAGVLGGFE 240 L P + + Y+T DP K + GF+ Sbjct: 311 --WLDWPRLSTN------------------------SVYATLDPLKLFFHLRTAQFSGFD 344 Query: 241 LQRRLEEKGMVPEMSDDRHVVLAFSLGSKAEDTQRL 276 LQ+ +E+ G+ E++DD HV+LA S G+ + D + L Sbjct: 345 LQKWMEKHGIFSELADDSHVLLAASTGTASRDLEAL 380 >Q5L3X0.1 Q5L3X0_GEOKA Lysine decarboxylase Length = 477 Score = 141 bits (355), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 87/229 (37%), Positives = 114/229 (49%), Gaps = 41/229 (17%) Query: 45 TMPNYYGMGSDLAPLARACHTSGVPLLVDEAHGAHYGQHPALPAGALMCGADGVVQSTHK 104 T PNYYGM +DL + R H G+P+LVDEAHGAH+ P P AL GAD VVQS HK Sbjct: 167 TNPNYYGMAADLTEIVRLVHEHGIPVLVDEAHGAHFVVGPPFPTSALRYGADVVVQSAHK 226 Query: 105 MLPALTMGAMLHVQGPWLDRTLLRQRLAMVQSSSPSYPVMASLDLARRLVHSQGAGAFTA 164 LPA+TMGA LHV +D L+ L + QSSSPSYP+MASLDLAR V A Sbjct: 227 TLPAMTMGAFLHVNSERVDLERLKYFLQLFQSSSPSYPIMASLDLARSYVAQLSKSDAAA 286 Query: 165 GLAAVDVLRRGLATLPRYGLLQPAVPLQGACXXXXXXXXXXXAAASTPPQGMAAYSTQ-- 222 +A ++ + LA + G+A ++ Sbjct: 287 IVAEIEKFKAALADI----------------------------------DGVAVVCSRHF 312 Query: 223 ----DPFKAVIYDAAGVLGGFELQRRLEEKGMVPEMSDDRHVVLAFSLG 267 DP K ++ L G+ELQR LE +G+ E++D +V+ L Sbjct: 313 GVQTDPLKVMVQTRCS-LTGYELQRWLEREGVFVELADRMNVLFVCPLA 360 >C3J4M2.1 C3J4M2_9BACI Orn/Lys/Arg decarboxylase major region Length = 477 Score = 141 bits (355), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 87/229 (37%), Positives = 113/229 (49%), Gaps = 41/229 (17%) Query: 45 TMPNYYGMGSDLAPLARACHTSGVPLLVDEAHGAHYGQHPALPAGALMCGADGVVQSTHK 104 T PNYYGM +DL + R H G+P+LVDEAHGAH+ P P AL GAD VVQS HK Sbjct: 167 TNPNYYGMAADLTEIVRLVHEHGIPVLVDEAHGAHFVVGPPFPTSALRYGADVVVQSAHK 226 Query: 105 MLPALTMGAMLHVQGPWLDRTLLRQRLAMVQSSSPSYPVMASLDLARRLVHSQGAGAFTA 164 LPA+TMGA LHV +D L+ L + QSSSPSYP+MASLDLAR V A Sbjct: 227 TLPAMTMGAFLHVNSERVDLERLKYFLQLFQSSSPSYPIMASLDLARSYVAQLSKSDAAA 286 Query: 165 GLAAVDVLRRGLATLPRYGLLQPAVPLQGACXXXXXXXXXXXAAASTPPQGMAAYSTQ-- 222 +A ++ + LA + G+A ++ Sbjct: 287 IVAEIEKFKAALADI----------------------------------DGVAVVCSRHF 312 Query: 223 ----DPFKAVIYDAAGVLGGFELQRRLEEKGMVPEMSDDRHVVLAFSLG 267 DP K + L G+ELQR LE +G+ E++D +V+ L Sbjct: 313 GVQTDPLKVTVQTRCS-LTGYELQRWLEREGVFVELADRMNVLFVCPLA 360 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,491,332,704 Number of extensions: 55792568 Number of successful extensions: 126032 Number of sequences better than 1.0e-05: 553 Number of HSP's gapped: 125905 Number of HSP's successfully gapped: 553 Length of query: 289 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 155 Effective length of database: 1,798,280,193 Effective search space: 278733429915 Effective search space used: 278733429915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)