BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0265.0023.p (300 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFI2.1 B3KFI2_9BACI Stage V sporulation protein B 333 2e-89 Q9KDI0.1 Q9KDI0_BACHD Involved in spore cortex synthesis 197 1e-48 C3H6Q1.1 C3H6Q1_BACTU Putative uncharacterized protein 193 2e-47 C3I6L2.1 C3I6L2_BACTU Stage V sporulation protein B 191 5e-47 B7IIS5.1 B7IIS5_BACC2 Stage V sporulation protein B 191 6e-47 >B3KFI2.1 B3KFI2_9BACI Stage V sporulation protein B Length = 532 Score = 333 bits (853), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 165/273 (60%), Positives = 209/273 (76%), Gaps = 6/273 (2%) Query: 1 LRKQSFIQGTLILLAAGIINRMLGFIPRIILPRVIGAEGVGLYQLGYPFFIVLITIITGG 60 + KQSFI+GTLILLAAGI+NR+LGFIPRI LPR+IG EGVG+YQLGYPFFIVL+TIITGG Sbjct: 1 MNKQSFIKGTLILLAAGILNRLLGFIPRIALPRIIGPEGVGIYQLGYPFFIVLVTIITGG 60 Query: 61 IPLAIAKMVAEAEGEGRPEESRRIXXXXXXXXXXXXVFFTLLALFSASWVSNVILTDSRV 120 IPLAIAKMVAEAEG G+P+ S++I FT L+L A WV+ V+L D RV Sbjct: 61 IPLAIAKMVAEAEGAGKPDASKQILHVSLMLTITAGALFTGLSLLLAPWVTGVLLPDERV 120 Query: 121 YYTFVSMTPMIAIVAVSAIYRGYFQGRQNMIPSALSSVLESVIRIVFMLWFSWLLLPRGV 180 Y TF+SMTPM+ I+AVS++YRGYFQG+QNMIPSA SSV+E+++RI+ MLWF+ LL+P+G+ Sbjct: 121 YQTFISMTPMMIIIAVSSVYRGYFQGKQNMIPSASSSVIETIVRIICMLWFAHLLMPKGI 180 Query: 181 AYAAAGAMLGVTVGEIVGMLALLWQYYYIVKRDKKADLSPTASTPDPAPENEEKTAASAK 240 AY AAGAMLG VGE++GM+ALL QY +R K P + +E+K S+ Sbjct: 181 AYGAAGAMLGTAVGELIGMIALLLQYSGEGRRTNKLLPKPQEAV------SEKKAPDSSS 234 Query: 241 SHSIFKRLIRISVPVTAGRLVGSFSYLLESIIT 273 + I KRL I++PVT G++VGSFSYLLE+I+T Sbjct: 235 NKGILKRLFGIALPVTGGKMVGSFSYLLETILT 267 >Q9KDI0.1 Q9KDI0_BACHD Involved in spore cortex synthesis Length = 522 Score = 197 bits (501), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 108/272 (39%), Positives = 162/272 (59%), Gaps = 16/272 (5%) Query: 1 LRKQSFIQGTLILLAAGIINRMLGFIPRIILPRVIGAEGVGLYQLGYPFFIVLITIITGG 60 + KQ+F++GT IL+ AG+I R LGF+ RI++ R++GAEGVGLY + P +++ITI G Sbjct: 1 MSKQTFLKGTFILIIAGLITRFLGFVNRIVVARIMGAEGVGLYVMAVPTLLLVITITQFG 60 Query: 61 IPLAIAKMVAEAEGEGRPEESRRIXXXXXXXXXXXXVFFTLLALFSASWVSNVILTDSRV 120 +P+AI+K+VAEA+ +RI + FT + A +++ +LTDSR Sbjct: 61 LPVAISKLVAEADALNDRSRIKRILVVSTTITLTLSIVFTTAMILLAPMIASTLLTDSRA 120 Query: 121 YYTFVSMTPMIAIVAVSAIYRGYFQGRQNMIPSALSSVLESVIRIVFMLWFSWLLLPRGV 180 Y+ V+++P++ IVA+S++ RGYFQG QNM P+A S V+E V+RI F+ + LP GV Sbjct: 121 YWPLVAISPIVPIVALSSVMRGYFQGLQNMKPTAYSQVIEQVVRITFVALLTSAFLPLGV 180 Query: 181 AYAAAGAMLGVTVGEIVGMLALLWQYYYIVKRDKKADLSPTASTPDPAPENEEKTAASAK 240 YAAAGAM+ V +GE+ +L ++ + KR K + ++ A + Sbjct: 181 EYAAAGAMISVVLGELASLLYMIVMF----KRKKSFRI------------RQKFFAYLGE 224 Query: 241 SHSIFKRLIRISVPVTAGRLVGSFSYLLESII 272 F L+ ISVP T RLVGS SY E I+ Sbjct: 225 GKKTFVDLMAISVPTTGSRLVGSVSYFFEPIV 256 >C3H6Q1.1 C3H6Q1_BACTU Putative uncharacterized protein Length = 519 Score = 193 bits (490), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 104/272 (38%), Positives = 156/272 (57%), Gaps = 16/272 (5%) Query: 1 LRKQSFIQGTLILLAAGIINRMLGFIPRIILPRVIGAEGVGLYQLGYPFFIVLITIITGG 60 + +QSF++G IL+ AG I ++LGFI RI++ R++G EGVGLY + P FI+ IT+ G Sbjct: 1 MTRQSFLKGAFILMIAGFITKILGFINRIVMARILGEEGVGLYMMAVPTFILAITLTQIG 60 Query: 61 IPLAIAKMVAEAEGEGRPEESRRIXXXXXXXXXXXXVFFTLLALFSASWVSNVILTDSRV 120 +P+AIAK VAEAE + ++I + T+ +F ++ +LTD R Sbjct: 61 LPVAIAKFVAEAEAVNDKQRVKKILTVSLAVTSVISIILTIGIMFLTPILAKTLLTDERT 120 Query: 121 YYTFVSMTPMIAIVAVSAIYRGYFQGRQNMIPSALSSVLESVIRIVFMLWFSWLLLPRGV 180 YY +++ P++ ++AVS++ RGYFQG+QNM PSA + VLE V+RI + L LP G+ Sbjct: 121 YYPLMAILPVVPVIAVSSVLRGYFQGKQNMKPSAYAQVLEQVVRITIIAICIQLFLPYGI 180 Query: 181 AYAAAGAMLGVTVGEIVGMLALLWQYYYIVKRDKKADLSPTASTPDPAPENEEKTAASAK 240 YAAAGAML +GE+ +L LL + +R+K + E + Sbjct: 181 EYAAAGAMLSAVLGEVASLLFLLTLF----QREKHLSI------------RSEFFTTVKE 224 Query: 241 SHSIFKRLIRISVPVTAGRLVGSFSYLLESII 272 S F L+ I++P T RL+GS SY E I+ Sbjct: 225 SKGTFYSLMDIALPTTGSRLIGSVSYFFEPIV 256 >C3I6L2.1 C3I6L2_BACTU Stage V sporulation protein B Length = 519 Score = 191 bits (486), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 104/272 (38%), Positives = 156/272 (57%), Gaps = 16/272 (5%) Query: 1 LRKQSFIQGTLILLAAGIINRMLGFIPRIILPRVIGAEGVGLYQLGYPFFIVLITIITGG 60 + +QSF++G IL+ AG I ++LGFI RI++ R++G EGVGLY + P FI+ IT+ G Sbjct: 1 MTRQSFLKGAFILMIAGFITKILGFINRIVMARILGEEGVGLYMMAVPTFILAITLTQIG 60 Query: 61 IPLAIAKMVAEAEGEGRPEESRRIXXXXXXXXXXXXVFFTLLALFSASWVSNVILTDSRV 120 +P+AIAK VAEAE + ++I + T+ +F ++ +LTD R Sbjct: 61 LPVAIAKFVAEAEAVNDKQRVKKILTVSLAVTSVISIILTIGIMFLTPILAKTLLTDERT 120 Query: 121 YYTFVSMTPMIAIVAVSAIYRGYFQGRQNMIPSALSSVLESVIRIVFMLWFSWLLLPRGV 180 YY +++ P++ ++A+S++ RGYFQG+QNM PSA + VLE V+RI + L LP GV Sbjct: 121 YYPLIAILPVVPVIAISSVLRGYFQGKQNMKPSAYAQVLEQVVRITIIAICIQLFLPYGV 180 Query: 181 AYAAAGAMLGVTVGEIVGMLALLWQYYYIVKRDKKADLSPTASTPDPAPENEEKTAASAK 240 YAAAGAML +GE+ +L LL + +R+K + T + Sbjct: 181 EYAAAGAMLSAVLGEVASLLFLLTLF----QREKHLSIRSGFFT------------TVKE 224 Query: 241 SHSIFKRLIRISVPVTAGRLVGSFSYLLESII 272 S F L+ I++P T RL+GS SY E I+ Sbjct: 225 SKGTFYSLMDIALPTTGSRLIGSVSYFFEPIV 256 >B7IIS5.1 B7IIS5_BACC2 Stage V sporulation protein B Length = 519 Score = 191 bits (486), Expect = 6e-47, Method: Compositional matrix adjust. Identities = 104/272 (38%), Positives = 156/272 (57%), Gaps = 16/272 (5%) Query: 1 LRKQSFIQGTLILLAAGIINRMLGFIPRIILPRVIGAEGVGLYQLGYPFFIVLITIITGG 60 + +QSF++G IL+ AG I ++LGFI RI++ R++G EGVGLY + P FI+ IT+ G Sbjct: 1 MTRQSFLKGAFILMIAGFITKILGFINRIVMARILGEEGVGLYMMAVPTFILAITLTQIG 60 Query: 61 IPLAIAKMVAEAEGEGRPEESRRIXXXXXXXXXXXXVFFTLLALFSASWVSNVILTDSRV 120 +P+AIAK VAEAE + ++I + T+ +F ++ +LTD R Sbjct: 61 LPVAIAKFVAEAEAVNDKQRVKKILTVSLAVTSVISIILTIGIMFLTPILAKTLLTDERT 120 Query: 121 YYTFVSMTPMIAIVAVSAIYRGYFQGRQNMIPSALSSVLESVIRIVFMLWFSWLLLPRGV 180 YY +++ P++ ++A+S++ RGYFQG+QNM PSA + VLE V+RI + L LP GV Sbjct: 121 YYPLIAILPVVPVIAISSVLRGYFQGKQNMKPSAYAQVLEQVVRITIIAICIQLFLPYGV 180 Query: 181 AYAAAGAMLGVTVGEIVGMLALLWQYYYIVKRDKKADLSPTASTPDPAPENEEKTAASAK 240 YAAAGAML +GE+ +L LL + +R+K + T + Sbjct: 181 EYAAAGAMLSAVLGEVASLLFLLTLF----QREKHLSIRSGFFT------------TVKE 224 Query: 241 SHSIFKRLIRISVPVTAGRLVGSFSYLLESII 272 S F L+ I++P T RL+GS SY E I+ Sbjct: 225 SKGTFYSLMDIALPTTGSRLIGSVSYFFEPIV 256 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.140 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,540,560,590 Number of extensions: 59501953 Number of successful extensions: 260527 Number of sequences better than 1.0e-05: 965 Number of HSP's gapped: 259380 Number of HSP's successfully gapped: 965 Length of query: 300 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 165 Effective length of database: 1,788,859,178 Effective search space: 295161764370 Effective search space used: 295161764370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)