BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0265.0020.p (460 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFI4.1 B3KFI4_9BACI Protein-export membrane protein SecD 560 e-157 B1DDN1.1 B1DDN1_9BACL Protein-export membrane protein SecD 429 e-118 C0ZAP6.1 C0ZAP6_BREBN Protein-export membrane protein SecD 332 5e-89 Q92BI7.1 Q92BI7_LISIN Lin1562 protein 328 7e-88 Q8Y703.1 Q8Y703_LISMO Lmo1527 protein 328 7e-88 >B3KFI4.1 B3KFI4_9BACI Protein-export membrane protein SecD Length = 416 Score = 560 bits (1443), Expect = e-157, Method: Compositional matrix adjust. Identities = 281/417 (67%), Positives = 337/417 (80%), Gaps = 1/417 (0%) Query: 44 MKRMLSFIITVLVLTGVMVFTTPGLLDKVRLGLDLKGGFEILYHAEPMEAGAELSRASLQ 103 MKR++SF+ TVLV+T +M TTPGLL VRLGLDLKGGFEILY AEP+ G ++++ +LQ Sbjct: 1 MKRIISFVGTVLVITILMAVTTPGLLKDVRLGLDLKGGFEILYEAEPLMEGQQVTKEALQ 60 Query: 104 KTAESLEKRANALGTSEPEVTTEGTDRIRLKIAGVTDEAEVRKKMKEPAVLTFRSAAPGD 163 +TA+SL+KRA+A+GTSEPEVT EG +RIRLK+AGVTDEAEVRK MKEPA LTFRS Sbjct: 61 ETAKSLQKRADAMGTSEPEVTPEGANRIRLKVAGVTDEAEVRKTMKEPANLTFRSNDGCK 120 Query: 164 PQGTFSKIELVGSDFVEGAAEVQRNNLNQPEISIKIKDKDKFAEITKRLLGKEMAIYLDD 223 F KIEL G DFVE A V + LNQP I IK+KDK KFAEIT+RLLG+E+AI++DD Sbjct: 121 EGEEFCKIELRGDDFVENGASVAYDQLNQPMIDIKVKDKGKFAEITERLLGQELAIFMDD 180 Query: 224 QLLGNPPVVRAVLTDGTASISGNYTIDGARELADTINLGALPLKLTEKYSQSVGATLGKQ 283 QLL + P VRAVLTDGTA I+GNY++D A+++ DTINLGALPLKL EKYSQSVGATLG Q Sbjct: 181 QLL-SAPTVRAVLTDGTAQITGNYSLDEAKQIRDTINLGALPLKLVEKYSQSVGATLGMQ 239 Query: 284 SLDQTIKAGLVGSIIILIFMIGMYRLPGLLASFALILHTWLLIAVFVVADFTLTLPGIAA 343 SL +T++AGL+ S+ IL+FMI YR+PGLLASFALILHTWLLI +FV ADFTLTLPGIAA Sbjct: 240 SLTKTLEAGLIASVAILLFMIIRYRVPGLLASFALILHTWLLILIFVWADFTLTLPGIAA 299 Query: 344 FILGIGMAVDANIITNERIREEMRNGKSILSSVKAGNKTSFRTVMDANVTTIIVAAVMFA 403 FILGIGMAVDANIIT ERIREE++ GKS+ S+VK+G K+S R +MD+N+TTI+VAAVMF Sbjct: 300 FILGIGMAVDANIITYERIREEIKTGKSVPSAVKSGGKSSLRAIMDSNLTTILVAAVMFG 359 Query: 404 FGTGAVKGFAXXXXXXXXXXXXTNLYFAHWLLTVLVKAGALKKPKQFGVKESDIRAL 460 FGTGAVKGFA TN+YF+ +LL +LV G +KP +GVKE DIR L Sbjct: 360 FGTGAVKGFALVLILEIIVSIATNVYFSRFLLNLLVNIGWARKPSHYGVKERDIREL 416 >B1DDN1.1 B1DDN1_9BACL Protein-export membrane protein SecD Length = 410 Score = 429 bits (1103), Expect = e-118, Method: Compositional matrix adjust. Identities = 219/418 (52%), Positives = 310/418 (74%), Gaps = 9/418 (2%) Query: 43 SMKRMLSFIITVLVLTGVMVFTTPGLLDKVRLGLDLKGGFEILYHAEPMEAGAELSRASL 102 + KR+ +F+ V+++ G + +T+P L+ +RLGLDLKGGFE+LY A P+E G +++ SL Sbjct: 2 NWKRLTAFLAVVVIMFGAIAWTSPALVKDIRLGLDLKGGFEVLYEASPLEGGEQVTSESL 61 Query: 103 QKTAESLEKRANALGTSEPEVTTEGTDRIRLKIAGVTDEAEVRKKMKEPAVLTFRSAAPG 162 ++TA SLE+R ++ GT+EPE+T EG++RIR+KI GVTDE +R+ +K+P LTFR Sbjct: 62 KETAHSLEQRIDSTGTTEPEITREGSNRIRVKIPGVTDETAIREMLKKPVNLTFRG---- 117 Query: 163 DPQGTFSKIELVGSDFVEGAAEVQRNNLNQPEISIKIKDKDKFAEITKRLLGKEMAIYLD 222 P GT +EL G+DFV+ A+V+ + L P I+IK+KD KF ++T +L GK ++IYLD Sbjct: 118 -PDGT---VELQGNDFVQNGAKVEFDQLGNPMITIKLKDAKKFEDVTTKLTGKTLSIYLD 173 Query: 223 DQLLGNPPVVRAVLTDGTASISGNYTIDGARELADTINLGALPLKLTEKYSQSVGATLGK 282 D+LL +P V + T G+A ISGNY++D A +L DTINLGALPLKLTE Y+QSVGA LG+ Sbjct: 174 DELLSSPSVNYPI-TGGSAQISGNYSLDEANKLKDTINLGALPLKLTEIYTQSVGAKLGQ 232 Query: 283 QSLDQTIKAGLVGSIIILIFMIGMYRLPGLLASFALILHTWLLIAVFVVADFTLTLPGIA 342 +SL+ T+ AG++G++IILI +I ++R+PG +A +I +TWLL+ VF + + TLTLPGIA Sbjct: 233 KSLEDTVGAGIIGTVIILILLIALFRVPGFVAGITVISYTWLLVVVFELMNATLTLPGIA 292 Query: 343 AFILGIGMAVDANIITNERIREEMRNGKSILSSVKAGNKTSFRTVMDANVTTIIVAAVMF 402 AF+LGIGMAVDANII ERI+EE+R+GKS+LSS+KAG+K SFRT+MDANVT II V++ Sbjct: 293 AFVLGIGMAVDANIIMYERIKEEIRSGKSLLSSLKAGSKNSFRTIMDANVTNIISCVVLY 352 Query: 403 AFGTGAVKGFAXXXXXXXXXXXXTNLYFAHWLLTVLVKAGALKKPKQFGVKESDIRAL 460 G GA++GFA TN++ A L+ +LV++ KP +GVKE++IRAL Sbjct: 353 FIGNGAIRGFALTMIFSILVSIVTNIFLARLLIHLLVRSNLFTKPGYYGVKEAEIRAL 410 >C0ZAP6.1 C0ZAP6_BREBN Protein-export membrane protein SecD Length = 412 Score = 332 bits (851), Expect = 5e-89, Method: Compositional matrix adjust. Identities = 180/398 (45%), Positives = 263/398 (66%), Gaps = 10/398 (2%) Query: 64 TTPGLLDKVRLGLDLKGGFEILYHAEPMEAGAELSRASLQKTAESLEKRANALGTSEPEV 123 TT + + LGLDL+GGFEILY EP+EAG ++ L+ TA +E+R N G +EP Sbjct: 24 TTKQVAGNITLGLDLQGGFEILYEVEPLEAGHKVDIELLKSTAHMIERRINIGGVAEPVT 83 Query: 124 TTEGTDRIRLKIAGVT-DEAEVRKKMKEPAVLTFRSAAPGDPQGTFSKIELVGSDFVEGA 182 TTE +RIR++IA + D+ ++R+ + +PA LTFR D G KI L GSD Sbjct: 84 TTELPNRIRVQIASQSADQDKLRELIGKPAHLTFR-----DENG---KIMLQGSDLAPNG 135 Query: 183 AEVQRNNLNQPEISIKIKDKDKFAEITKRLLGKEMAIYLDDQLLGNPPVVRAVLTDGTAS 242 A V ++L +P +++K D K E T+ L K MAI+LD+ + NP +++V++ G+A Sbjct: 136 AAVGYDDLKRPLVTLKFSDPKKLEEATRANLHKPMAIFLDETMQTNP-TIQSVISGGSAQ 194 Query: 243 ISGNYTIDGARELADTINLGALPLKLTEKYSQSVGATLGKQSLDQTIKAGLVGSIIILIF 302 I+GNYT + A+ELAD +N GA+P KL EK SVGA+LG +L++T+ AG +G+ +I IF Sbjct: 195 ITGNYTQESAQELADLLNSGAMPAKLVEKQVTSVGASLGTLALEKTMYAGYIGAALIFIF 254 Query: 303 MIGMYRLPGLLASFALILHTWLLIAVFVVADFTLTLPGIAAFILGIGMAVDANIITNERI 362 M+ +YR+PG++A+ L T+ + V TLTLPGIA FIL +G+AVDANIIT ERI Sbjct: 255 MLVVYRMPGMIANITLAGFTYFCMVVLDWMHATLTLPGIAGFILAVGIAVDANIITYERI 314 Query: 363 REEMRNGKSILSSVKAGNKTSFRTVMDANVTTIIVAAVMFAFGTGAVKGFAXXXXXXXXX 422 +EE+R+GK++LS+ +AG + S T++DA+VTT+I V+F FGT +V+GFA Sbjct: 315 QEEIRSGKTVLSAFRAGERRSLITIIDAHVTTLIATGVLFFFGTSSVQGFAVVLAMTIIV 374 Query: 423 XXXTNLYFAHWLLTVLVKAGALKKPKQFGVKESDIRAL 460 TN++ + +LL ++V++ KKP FGVKES+IR L Sbjct: 375 SIITNVFGSRFLLWLVVRSNMFKKPFWFGVKESEIREL 412 >Q92BI7.1 Q92BI7_LISIN Lin1562 protein Length = 754 Score = 328 bits (841), Expect = 7e-88, Method: Compositional matrix adjust. Identities = 180/434 (41%), Positives = 273/434 (62%), Gaps = 27/434 (6%) Query: 46 RMLSFIITVLVLTGVMVFTTPGLLDKVRLGLDLKGGFEILYHAEPMEAGAELSRASLQKT 105 +++ F + V ++ G + T ++ + LGLDL+GGFE+LY P + +S+ +L T Sbjct: 6 KIVIFFLVVAIIFGAVFGTAKMVMQNINLGLDLQGGFEVLYEVSPADGKGTVSKQTLTDT 65 Query: 106 AESLEKRANALGTSEPEVTTEGTDRIRLKIAGVTDEAEVRKKMKEPAVLTFRSAAPGDPQ 165 SL+KR N+LG +EP + EG +RIR+++AGVTD+AE RK + A L+FR A Sbjct: 66 VTSLDKRVNSLGVAEPSIQIEGNNRIRVQLAGVTDQAEARKMLSTTAQLSFRDAN----- 120 Query: 166 GTFSKIELVGSDFVEGAAEVQRNNLNQPEISIKIKDKDKFAEITKRLLGK----EMAIYL 221 K+ + GSD V G A+ + N P +++K+K DKFA +TK +L + ++ I+L Sbjct: 121 ---DKMMMNGSDLVAGGAKQAFDASNNPIVTLKLKSADKFASVTKTILAEAPDNQLVIWL 177 Query: 222 D---------DQLLGNP-----PVVRAVLTDGTASISGNYTIDGARELADTINLGALPLK 267 D ++ NP P V +VL ISG++T + A++LA+ +N GALP+K Sbjct: 178 DWKEGQKYKTEREKKNPAYLSAPNVSSVLDTDKVEISGSFTTEEAKDLAELLNSGALPVK 237 Query: 268 LTEKYSQSVGATLGKQSLDQTIKAGLVGSIIILIFMIGMYRLPGLLASFALILHTWLLIA 327 +TE YS SVGA G+ +L +TI AG++G + I IFM+ +YRLPG++AS L+ +T+L++ Sbjct: 238 MTEVYSTSVGAQFGQDALQETILAGIIGVMAIFIFMMAVYRLPGVIASITLVAYTYLVLL 297 Query: 328 VFVVADFTLTLPGIAAFILGIGMAVDANIITNERIREEMRNGKSILSSVKAGNKTSFRTV 387 + + + TLTLPGIA ILGIGMAVDAN+IT ERI+EE++ G+S ++ + G K +FR + Sbjct: 298 ILSLLNATLTLPGIAGLILGIGMAVDANVITYERIKEEIKVGRSTKAAFEVGGKEAFRAI 357 Query: 388 MDANVTTIIVAAVMFAFGTGAVKGFAXXXXXXXXXXXXTNLYFAHWLLTVLVKAGAL-KK 446 +D N+TT+IVAAV+F FGT ++KGFA T ++ + +LL +LVK+ L K Sbjct: 358 LDGNLTTLIVAAVLFYFGTSSIKGFATVLIISILVSFLTAVWGSRFLLGLLVKSNWLNNK 417 Query: 447 PKQFGVKESDIRAL 460 P F VK DI L Sbjct: 418 PGFFAVKRKDIHNL 431 >Q8Y703.1 Q8Y703_LISMO Lmo1527 protein Length = 754 Score = 328 bits (841), Expect = 7e-88, Method: Compositional matrix adjust. Identities = 179/434 (41%), Positives = 271/434 (62%), Gaps = 27/434 (6%) Query: 46 RMLSFIITVLVLTGVMVFTTPGLLDKVRLGLDLKGGFEILYHAEPMEAGAELSRASLQKT 105 +++ F + V ++ G + T ++ + LGLDL+GGFE+LY P + +S+ +L T Sbjct: 6 KIVIFFLVVAIIFGAVFGTAKMVMQNINLGLDLQGGFEVLYEVSPADGKGTVSKQTLTDT 65 Query: 106 AESLEKRANALGTSEPEVTTEGTDRIRLKIAGVTDEAEVRKKMKEPAVLTFRSAAPGDPQ 165 SL+KR N+LG +EP + EG +RIR+++AGVTD+AE RK + A L+FR A Sbjct: 66 VTSLDKRVNSLGVAEPSIQIEGNNRIRVQLAGVTDQAEARKMLSTTAQLSFRDAN----- 120 Query: 166 GTFSKIELVGSDFVEGAAEVQRNNLNQPEISIKIKDKDKFAEITKRLLGK----EMAIYL 221 K+ + GSD V G A+ ++ N P +++K+K DKFA +TK +L + ++ I+L Sbjct: 121 ---DKMMMNGSDLVAGGAKQAFDSSNNPIVTLKLKSADKFASVTKTILAEAPDNQLVIWL 177 Query: 222 D--------------DQLLGNPPVVRAVLTDGTASISGNYTIDGARELADTINLGALPLK 267 D D + P V +VL ISG++T + A++LA+ +N GALP+K Sbjct: 178 DWKEGQKYKTEREKKDPAYLSAPNVSSVLDTDKVEISGSFTTEEAKDLAELLNSGALPVK 237 Query: 268 LTEKYSQSVGATLGKQSLDQTIKAGLVGSIIILIFMIGMYRLPGLLASFALILHTWLLIA 327 + E YS SVGA G+ +L +TI AG++G I I IFM+ +YRLPG++AS L+ +T+L++ Sbjct: 238 MKEVYSTSVGAQFGQDALQETILAGIIGVIAIFIFMMAVYRLPGVIASITLVAYTYLVLL 297 Query: 328 VFVVADFTLTLPGIAAFILGIGMAVDANIITNERIREEMRNGKSILSSVKAGNKTSFRTV 387 + + + TLTLPGIA ILGIGMAVDAN+IT ERI+EE++ G+S ++ + G K +FR + Sbjct: 298 ILSLLNATLTLPGIAGLILGIGMAVDANVITYERIKEEIKVGRSTKAAFEVGGKEAFRAI 357 Query: 388 MDANVTTIIVAAVMFAFGTGAVKGFAXXXXXXXXXXXXTNLYFAHWLLTVLVKAGAL-KK 446 +D N+TT+IVAAV+F FGT ++KGFA T ++ + +LL +LVK+ L K Sbjct: 358 LDGNLTTLIVAAVLFYFGTSSIKGFATVLIISILVSFLTAVWGSRFLLGLLVKSNWLNNK 417 Query: 447 PKQFGVKESDIRAL 460 P F VK DI L Sbjct: 418 PGFFAVKRKDIHNL 431 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,436,713,592 Number of extensions: 96483860 Number of successful extensions: 332882 Number of sequences better than 1.0e-05: 1488 Number of HSP's gapped: 331076 Number of HSP's successfully gapped: 1563 Length of query: 460 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 321 Effective length of database: 1,751,175,118 Effective search space: 562127212878 Effective search space used: 562127212878 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)