BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0265.0019.p (300 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFI5.1 B3KFI5_9BACI Protein-export membrane protein SecF 415 e-114 B1DDN0.1 B1DDN0_9BACL Protein-export membrane protein SecF 278 6e-73 C3IY81.1 C3IY81_9BACI Protein-export membrane protein SecD 251 5e-65 B7Y5Z4.1 B7Y5Z4_9BACI Protein-export membrane protein SecD 251 5e-65 C1PSC2.1 C1PSC2_ALIAC Protein-export membrane protein SecF 246 2e-63 >B3KFI5.1 B3KFI5_9BACI Protein-export membrane protein SecF Length = 305 Score = 415 bits (1067), Expect = e-114, Method: Compositional matrix adjust. Identities = 202/297 (68%), Positives = 245/297 (82%), Gaps = 2/297 (0%) Query: 5 KELDFVHLSRFFYIFSIAITLAGLIFLATMGLNYSVDFKAGSNVDVALSKNITLEQLKPA 64 K DFV SR FY+ SI IT+ GL+FL T GLNY VDF++GSNVD++LSK +T EQ+ Sbjct: 4 KNFDFVGKSRIFYVLSIVITILGLVFLLTRGLNYGVDFQSGSNVDISLSKPLTTEQINTV 63 Query: 65 LKELGISHEPDITLGENRVNIRYDEVLDSNQDEALKAAVTKLDDKASFEINTVDTEMAKE 124 L++ GI + +T G +RVNIR+ EVL +QD+ L++ + KLD+KA+FEINTVD EMAKE Sbjct: 64 LEDAGIGEDASVTPGNDRVNIRFSEVLTEDQDQKLRSGIEKLDEKATFEINTVDPEMAKE 123 Query: 125 LARNAIYSVLISCLGIIIYVSIRFEWRFALAAIVALVHDAFIVVAVFSIFRLEVDLTFIV 184 L RNAIY+VLISC+GIIIYVSIRFEWRFA+AAIVAL+HDAF+V+++FSI LEVDLTFI+ Sbjct: 124 LGRNAIYAVLISCIGIIIYVSIRFEWRFAIAAIVALLHDAFVVISIFSILGLEVDLTFII 183 Query: 185 AVLTIIGYSINDTIVIFDRIRENLRFGKQKTYEDLKVLVNKSVAQTLMRSLYTAFTVFIA 244 AVLTIIGYSINDTIVIFDRIRENLRF K K DLK LVNKS++QT+ RS+YTA +VFIA Sbjct: 184 AVLTIIGYSINDTIVIFDRIRENLRFAKIKRVSDLKDLVNKSLSQTIGRSIYTALSVFIA 243 Query: 245 AFFLLVMGGESIKMFSLAMVIGLLFGAYSSIFIASPLWLLLKKRQ--KQPVKSNPAK 299 AFF+L++GGESI+MFSLA+ IGL FGAYSSIFIASPLWLLLK R +P P+K Sbjct: 244 AFFMLLLGGESIQMFSLAICIGLAFGAYSSIFIASPLWLLLKGRNVSNKPNSPKPSK 300 >B1DDN0.1 B1DDN0_9BACL Protein-export membrane protein SecF Length = 306 Score = 278 bits (710), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 144/300 (48%), Positives = 205/300 (68%), Gaps = 3/300 (1%) Query: 4 KKELDFVHLSRFFYIFSIAITLAGLIFLATMGLNYSVDFKAGSNVDVALSKNITLEQLKP 63 K DF+ F++FS +T+ G++ ++ LN+ VDFKAG+N+D+ + K I+ E+ K Sbjct: 6 KVRFDFIKHRNKFFMFSAILTIVGILMVSIFHLNFGVDFKAGTNMDILVGKKISQEEAKD 65 Query: 64 ALKELGISHEPDITLGEN--RVNIRYDEVLDSNQDEALKAAV-TKLDDKASFEINTVDTE 120 L + G S G N RV+ R+D+VL+ ++ + ++ A K D S E+N V + Sbjct: 66 ILSQSGYSDITPTVGGTNGDRVSARFDDVLEQSKVQVIEDAFKAKYGDNVSSEVNVVSPD 125 Query: 121 MAKELARNAIYSVLISCLGIIIYVSIRFEWRFALAAIVALVHDAFIVVAVFSIFRLEVDL 180 +A+EL AIY+V+I+ + I++YV IRFEWR ALAA +A+++DAF+V+ +FSIFRL+VDL Sbjct: 126 IAQELGVKAIYAVIIASVAIMLYVMIRFEWRMALAANIAILYDAFVVITMFSIFRLQVDL 185 Query: 181 TFIVAVLTIIGYSINDTIVIFDRIRENLRFGKQKTYEDLKVLVNKSVAQTLMRSLYTAFT 240 FI AVLT IGYSIND IVIFDRIRENLRF K K + + LVN+S+ QT+ R++YT Sbjct: 186 PFIAAVLTTIGYSINDKIVIFDRIRENLRFSKTKNADQIGDLVNQSLWQTMARNIYTVLA 245 Query: 241 VFIAAFFLLVMGGESIKMFSLAMVIGLLFGAYSSIFIASPLWLLLKKRQKQPVKSNPAKA 300 V I A L + G ESIK+F+LA +IGL GAYSSI IA+PLW+++K +QK K+ KA Sbjct: 246 VLIIATCLFIFGSESIKLFALAKMIGLASGAYSSICIAAPLWMVMKSKQKPSTKAAAKKA 305 >C3IY81.1 C3IY81_9BACI Protein-export membrane protein SecD Length = 750 Score = 251 bits (642), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 143/287 (49%), Positives = 198/287 (68%), Gaps = 9/287 (3%) Query: 8 DFVHLSRFFYIFSIAITLAGLIFLATMGLNYSVDFKAGSNVDVALSKNITLEQLKPALKE 67 DFV S+ F+IFS A+T+AG+I L +GLN +DF +G+ ++V S+ I ++L + Sbjct: 450 DFVKHSQKFFIFSGALTIAGVISLFALGLNLGIDFTSGTRIEVTSSQPIDAKELSGYFER 509 Query: 68 LGISHEP-DITL----GENRVNIRYDEVLDSNQDEALKAAVTKLDDKASFEINTVDTEMA 122 LG H+P D+ L G+ V +R VLD + LK + K + I+TV + Sbjct: 510 LG--HKPEDVVLSGTDGKTGV-VRLIGVLDKQEIAKLKNEL-KQAYGSEPNISTVSPVVG 565 Query: 123 KELARNAIYSVLISCLGIIIYVSIRFEWRFALAAIVALVHDAFIVVAVFSIFRLEVDLTF 182 K+LARNA+ +VLIS +GII+YV+IRFEWR ALAAIVAL+HDAF ++ VFSI RLEVDLTF Sbjct: 566 KQLARNALIAVLISSIGIILYVTIRFEWRMALAAIVALLHDAFFIITVFSITRLEVDLTF 625 Query: 183 IVAVLTIIGYSINDTIVIFDRIRENLRFGKQKTYEDLKVLVNKSVAQTLMRSLYTAFTVF 242 I AVLTIIGYSINDTIV FDRIR+ ++ K KT +DLK +VN+++ QT RS+ T TV Sbjct: 626 IAAVLTIIGYSINDTIVTFDRIRDLMKKRKVKTVDDLKRIVNRALQQTFTRSVNTVLTVL 685 Query: 243 IAAFFLLVMGGESIKMFSLAMVIGLLFGAYSSIFIASPLWLLLKKRQ 289 LL+ G E+I+ F+LA+++GL+ G YSS+ IAS LW++ K ++ Sbjct: 686 FTVIALLIFGSEAIRNFNLALLVGLVCGVYSSLCIASQLWVVWKGQE 732 >B7Y5Z4.1 B7Y5Z4_9BACI Protein-export membrane protein SecD Length = 750 Score = 251 bits (642), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 143/287 (49%), Positives = 198/287 (68%), Gaps = 9/287 (3%) Query: 8 DFVHLSRFFYIFSIAITLAGLIFLATMGLNYSVDFKAGSNVDVALSKNITLEQLKPALKE 67 DFV S+ F+IFS A+T+AG+I L +GLN +DF +G+ ++V S+ I ++L + Sbjct: 450 DFVKHSQKFFIFSGALTIAGVISLFALGLNLGIDFTSGTRIEVTSSQPIDAKELSGYFER 509 Query: 68 LGISHEP-DITL----GENRVNIRYDEVLDSNQDEALKAAVTKLDDKASFEINTVDTEMA 122 LG H+P D+ L G+ V +R VLD + LK + K + I+TV + Sbjct: 510 LG--HKPEDVVLSGTDGKTGV-VRLIGVLDKQEIAKLKNEL-KQAYGSEPNISTVSPVVG 565 Query: 123 KELARNAIYSVLISCLGIIIYVSIRFEWRFALAAIVALVHDAFIVVAVFSIFRLEVDLTF 182 K+LARNA+ +VLIS +GII+YV+IRFEWR ALAAIVAL+HDAF ++ VFSI RLEVDLTF Sbjct: 566 KQLARNALIAVLISSIGIILYVTIRFEWRMALAAIVALLHDAFFIITVFSITRLEVDLTF 625 Query: 183 IVAVLTIIGYSINDTIVIFDRIRENLRFGKQKTYEDLKVLVNKSVAQTLMRSLYTAFTVF 242 I AVLTIIGYSINDTIV FDRIR+ ++ K KT +DLK +VN+++ QT RS+ T TV Sbjct: 626 IAAVLTIIGYSINDTIVTFDRIRDLMKKRKVKTVDDLKRIVNRALQQTFTRSVNTVLTVL 685 Query: 243 IAAFFLLVMGGESIKMFSLAMVIGLLFGAYSSIFIASPLWLLLKKRQ 289 LL+ G E+I+ F+LA+++GL+ G YSS+ IAS LW++ K ++ Sbjct: 686 FTVIALLIFGSEAIRNFNLALLVGLVCGVYSSLCIASQLWVVWKGQE 732 >C1PSC2.1 C1PSC2_ALIAC Protein-export membrane protein SecF Length = 308 Score = 246 bits (628), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 133/291 (45%), Positives = 198/291 (68%), Gaps = 6/291 (2%) Query: 4 KKELDFVHLSRFFYIFSIAITLAGLIFLATMGLNYSVDFKAGSNVDVALSKNITLEQLKP 63 K D + SR+F++ S AIT+AG+I A G N S DFK+GS V L++ + +++ Sbjct: 2 KPRFDIIRASRWFFLLSGAITVAGVIVFALFGFNLSTDFKSGSEVQFELNQRVPEARVRQ 61 Query: 64 ALKELGIS-HEPDITLG---ENRVNIRYDEVLDSNQDEALKAAVTKL--DDKASFEINTV 117 +G+ + +T+G +N V + E L + Q ++AA + D K ++N+V Sbjct: 62 MFASIGLPLGDTSLTVGGIQQNVVMVTLPEQLTAKQISEIQAAEHRFFPDAKQDIQVNSV 121 Query: 118 DTEMAKELARNAIYSVLISCLGIIIYVSIRFEWRFALAAIVALVHDAFIVVAVFSIFRLE 177 D +A++ AR A+Y+VL + I++Y++IRFE+RFA++ I+AL+HDAFIV+A F++ R E Sbjct: 122 DPFVAEQTARKAVYAVLAAAACIVVYIAIRFEFRFAISGIIALLHDAFIVLAAFALLRRE 181 Query: 178 VDLTFIVAVLTIIGYSINDTIVIFDRIRENLRFGKQKTYEDLKVLVNKSVAQTLMRSLYT 237 VDLTF+ A+LTI+GYSINDTIVIFDRIRENL+ K +T ++L+ +VNKS+ Q + RS+ T Sbjct: 182 VDLTFVAALLTIVGYSINDTIVIFDRIRENLKIDKPETVDELRAVVNKSLWQVMNRSIRT 241 Query: 238 AFTVFIAAFFLLVMGGESIKMFSLAMVIGLLFGAYSSIFIASPLWLLLKKR 288 TV IAA L GG SI+ F+ A++IGL+ GAYSSIFIASP+W+ + R Sbjct: 242 VLTVLIAAVILYFFGGISIRNFTFALIIGLVSGAYSSIFIASPIWVAWRSR 292 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.140 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,538,370,424 Number of extensions: 57834613 Number of successful extensions: 280783 Number of sequences better than 1.0e-05: 1910 Number of HSP's gapped: 278613 Number of HSP's successfully gapped: 2029 Length of query: 300 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 165 Effective length of database: 1,788,859,178 Effective search space: 295161764370 Effective search space used: 295161764370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)