BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0265.0018.p (309 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFI6.1 B3KFI6_9BACI Cation diffusion facilitator family transp... 322 3e-86 Q9KEY7.1 Q9KEY7_BACHD Cation efflux system 167 9e-40 A8VU28.1 A8VU28_9BACI Co/Zn/Cd cation transporter-like protein 166 2e-39 C2ZUT0.1 C2ZUT0_BACCE Cation efflux system 159 3e-37 C2ZD96.1 C2ZD96_BACCE Cation efflux system 159 3e-37 >B3KFI6.1 B3KFI6_9BACI Cation diffusion facilitator family transporter Length = 321 Score = 322 bits (824), Expect = 3e-86, Method: Compositional matrix adjust. Identities = 160/314 (50%), Positives = 220/314 (70%), Gaps = 9/314 (2%) Query: 1 MVWTGVASDLALAAAKGTMGYLSGSKALMGDALYSGSDAAAKLAEILP---FPWRREPEK 57 ++WTG+ +DLALA KG GYLSGSKAL+GDAL+S ++A L + LP + P + Sbjct: 12 VLWTGIVTDLALALCKGIAGYLSGSKALIGDALHSAANAVTLLVDRLPSLGIFGTKSPMR 71 Query: 58 KNRIAPRNPQNNKGPIIAILFSVLILMGGLQIAFSAIRDLTRGHLSAPGQTALITVLISI 117 R N + P+I+ILF+VL++MGG+QIA SAI+ + +L P +AL+ + IS+ Sbjct: 72 DKR----NVSSTTEPVISILFAVLLIMGGIQIAVSAIQTMASDNLEPPATSALLAIFISM 127 Query: 118 VVKEAVFQYQYRYFKKLGDGSHAAYADNHRFSLYTSITVFIGISLAMAGGAFNLHPLLYM 177 V EAVFQYQ K+L D + + +HRF+LY+S+TV IG+SL+MAG F PL YM Sbjct: 128 AVNEAVFQYQSWQMKRLHDPRYEMLSADHRFALYSSLTVLIGVSLSMAGSYFEWSPLFYM 187 Query: 178 DPIAALLTGCLIIRKGYSLIATSVYGKKLQELPSEEAAGFIETVQRVHGVIRVEYLQALE 237 D IAAL+ CL++RKGY LI SVYG +E E A F++T+QRVHGVI +E L+ E Sbjct: 188 DSIAALIVACLVVRKGYILIVHSVYGTLAEEKRHEHEAEFMDTIQRVHGVITIEELKVQE 247 Query: 238 QG--RYVNLQVKISVNPRITVMEAQDISECARKLLQHRFVHVGEVHMDVVPYDPGYPYKS 295 G R+VN++VKI+VNPR +V+EAQ+I+E RKLL HRF+ V + + VVPY+PGYPYKS Sbjct: 248 YGQIRHVNIEVKITVNPRTSVLEAQEIAERIRKLLTHRFIQVTDAKVHVVPYEPGYPYKS 307 Query: 296 NHELVDNEIPTLLQ 309 N++L+DN++PTL Q Sbjct: 308 NYDLMDNDMPTLPQ 321 >Q9KEY7.1 Q9KEY7_BACHD Cation efflux system Length = 307 Score = 167 bits (424), Expect = 9e-40, Method: Compositional matrix adjust. Identities = 96/285 (33%), Positives = 156/285 (54%), Gaps = 3/285 (1%) Query: 3 WTGVASDLALAAAKGTMGYLSGSKALMGDALYSGSDAAAKLAEILPFPWRREPEKKNRIA 62 W G+ + LA KG +G+LSGS+AL+ DA +S SD A +A + ++P + Sbjct: 13 WVGIIVNGLLALMKGVIGWLSGSRALIADAAHSASDVAGSIAVLAGLRTAKKPPDADHPY 72 Query: 63 PRNPQNNKGPIIAILFSVLILMGGLQIAFSAIRDLTRGHLSAPGQTALITVLISIVVKEA 122 N + I+ ++L+++ G++IA S+ + SAP AL+ ++ SI++KE Sbjct: 73 GHGKAEN---VATIIVAILLIVVGVEIAISSTKVFVGEMPSAPKGIALVAIIASIIIKEV 129 Query: 123 VFQYQYRYFKKLGDGSHAAYADNHRFSLYTSITVFIGISLAMAGGAFNLHPLLYMDPIAA 182 +FQY+YR KK+ + A A +HR +SI FIG+ AM G FN L+Y+DP+A Sbjct: 130 LFQYKYRLAKKINSSALLAEAWHHRSDALSSIAAFIGVFGAMVGQHFNYPILVYLDPVAG 189 Query: 183 LLTGCLIIRKGYSLIATSVYGKKLQELPSEEAAGFIETVQRVHGVIRVEYLQALEQGRYV 242 L+ ++I+ G+SL S Q L E+ FI TV ++ GV R++ L A G Y+ Sbjct: 190 LIVSLIVIKIGFSLAKESSSIMMEQVLDPEKTKPFIRTVSKIEGVKRIDELLARTHGHYI 249 Query: 243 NLQVKISVNPRITVMEAQDISECARKLLQHRFVHVGEVHMDVVPY 287 + +K+ V+P ++V E IS+ +K L H+ V + + PY Sbjct: 250 VVDIKVGVDPNLSVEEGHRISKRVKKALLANHNHIKRVFVHINPY 294 >A8VU28.1 A8VU28_9BACI Co/Zn/Cd cation transporter-like protein Length = 315 Score = 166 bits (420), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 99/287 (34%), Positives = 155/287 (54%), Gaps = 3/287 (1%) Query: 3 WTGVASDLALAAAKGTMGYLSGSKALMGDALYSGSDAAAKLAEILPFPWRREPEKKNRIA 62 W G+ ++ LA KG G+LSGS+AL+ DA +S SD A +A + ++P K+ Sbjct: 13 WIGIIANAILAILKGITGWLSGSRALIADAAHSASDVAGSIAVLAGLKTAQKPPDKDHPY 72 Query: 63 PRNPQNNKGPIIAILFSVLILMGGLQIAFSAIRDLTRGHLSAPGQTALITVLISIVVKEA 122 N I I+ ++L+++ G +IA S+I G APG AL+ + S++ KE Sbjct: 73 GHGKAEN---IATIIVAILLIVVGFEIAISSIGAFFGGVTRAPGIMALVVIFFSLITKEI 129 Query: 123 VFQYQYRYFKKLGDGSHAAYADNHRFSLYTSITVFIGISLAMAGGAFNLHPLLYMDPIAA 182 ++ Y+ R KK+ + A A +HR +S+ FIGI+ AM G A NL L+Y+DP+A Sbjct: 130 LYHYKSRLAKKINSSALMAEAWHHRSDSISSLAAFIGIAGAMFGQAMNLPLLVYLDPLAG 189 Query: 183 LLTGCLIIRKGYSLIATSVYGKKLQELPSEEAAGFIETVQRVHGVIRVEYLQALEQGRYV 242 L L+++ G++L S Q L +E E+ + V GVIRV+ L A G Y+ Sbjct: 190 LAVSVLVMKVGFTLAKESSQVMLEQVLSDDEVKPIAESAENVEGVIRVDELLARTHGHYI 249 Query: 243 NLQVKISVNPRITVMEAQDISECARKLLQHRFVHVGEVHMDVVPYDP 289 + +K+SV+P I+V + IS+ +K L + V V + V PY P Sbjct: 250 IVDIKVSVDPSISVEKGHTISKNVKKCLIKNYDTVQNVFVHVNPYRP 296 >C2ZUT0.1 C2ZUT0_BACCE Cation efflux system Length = 297 Score = 159 bits (402), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 100/292 (34%), Positives = 155/292 (53%), Gaps = 17/292 (5%) Query: 4 TGVASDLALAAAKGTMGYLSGSKALMGDALYSGSDAAAKLAEILPFPWRREPEKKNRIAP 63 G+ ++ LA K +GY+ SKAL+ DA++SGSD LA + ++P ++ P Sbjct: 16 VGIVGNIILAIIKAVIGYIGNSKALLADAVHSGSDVIGSLAVLFGLRAAKQPPDEDH--P 73 Query: 64 RNPQNNKGPIIAILFSVLILMGGLQIAFSAIRDLTRGHLSAPGQTALITVLISIVVKEAV 123 I AI+ +VL+ + GL+IA S+I+ ++ L P + V+ SIVVKE + Sbjct: 74 YG-HGKAESISAIIVAVLLFIVGLEIAISSIKAFSQ-ELEPPKGITIFAVIFSIVVKEGM 131 Query: 124 FQYQYRYFKKLGDGSHAAYADNHRFSLYTSITVFIGISLAMAGGAFNLHPLLYMDPIAAL 183 FQY++R K++ + A A HR +++SI IGI A+ GG F L L+Y DPIA L Sbjct: 132 FQYKFRLGKRINSDAIIANAYEHRSDVFSSIAALIGICAAIIGGKFGLDWLVYADPIAGL 191 Query: 184 LTGCLIIRKGYSLIATSVYGKKLQELPSEEAAGFIETVQRVHGVIRVEYLQALEQGRYVN 243 L+ + +S+ A +++ L E+ F E V +V GV ++ L A E G YV Sbjct: 192 FVSLLVAKMAWSIGAEAIHATLDHVLHEEDIIPFREAVLQVEGVKKIGSLYAREHGHYVI 251 Query: 244 LQVKISVNPRITVMEAQDISECARKLL------QHRFVHVGEVHMDVVPYDP 289 + +K+SV+P ITV E I + ++ L Q+ FVH+ PY P Sbjct: 252 VDIKVSVDPYITVEEGHRIGKHVKETLMKQDNVQNVFVHIN-------PYSP 296 >C2ZD96.1 C2ZD96_BACCE Cation efflux system Length = 297 Score = 159 bits (402), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 100/292 (34%), Positives = 155/292 (53%), Gaps = 17/292 (5%) Query: 4 TGVASDLALAAAKGTMGYLSGSKALMGDALYSGSDAAAKLAEILPFPWRREPEKKNRIAP 63 G+ ++ LA K +GY+ SKAL+ DA++SGSD LA + ++P ++ P Sbjct: 16 VGIVGNIILAIIKAVIGYIGNSKALLADAVHSGSDVIGSLAVLFGLRAAKQPPDEDH--P 73 Query: 64 RNPQNNKGPIIAILFSVLILMGGLQIAFSAIRDLTRGHLSAPGQTALITVLISIVVKEAV 123 I AI+ +VL+ + GL+IA S+I+ ++ L P + V+ SIVVKE + Sbjct: 74 YG-HGKAESISAIIVAVLLFIVGLEIAISSIKAFSQ-ELEPPKGITIFAVIFSIVVKEGM 131 Query: 124 FQYQYRYFKKLGDGSHAAYADNHRFSLYTSITVFIGISLAMAGGAFNLHPLLYMDPIAAL 183 FQY++R K++ + A A HR +++SI IGI A+ GG F L L+Y DPIA L Sbjct: 132 FQYKFRLGKRINSDAIIANAYEHRSDVFSSIAALIGICAAIIGGKFGLDWLVYADPIAGL 191 Query: 184 LTGCLIIRKGYSLIATSVYGKKLQELPSEEAAGFIETVQRVHGVIRVEYLQALEQGRYVN 243 L+ + +S+ A +++ L E+ F E V +V GV ++ L A E G YV Sbjct: 192 FVSLLVAKMAWSIGAEAIHATLDHVLHEEDIIPFREAVLQVEGVKKIGSLYAREHGHYVI 251 Query: 244 LQVKISVNPRITVMEAQDISECARKLL------QHRFVHVGEVHMDVVPYDP 289 + +K+SV+P ITV E I + ++ L Q+ FVH+ PY P Sbjct: 252 VDIKVSVDPYITVEEGHRIGKHVKETLMKQDNVQNVFVHIN-------PYSP 296 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.138 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,820,840,602 Number of extensions: 72045338 Number of successful extensions: 195391 Number of sequences better than 1.0e-05: 712 Number of HSP's gapped: 195093 Number of HSP's successfully gapped: 712 Length of query: 309 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 174 Effective length of database: 1,788,859,178 Effective search space: 311261496972 Effective search space used: 311261496972 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)