BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0062.p (323 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG70.1 B3KG70_9BACI 2-dehydropantoate 2-reductase EC=1.1.1.169 278 7e-73 B1DBW5.1 B1DBW5_9BACL 2-dehydropantoate 2-reductase EC=1.1.1.169 169 2e-40 C0ZGC2.1 C0ZGC2_BREBN 2-dehydropantoate 2-reductase EC=1.1.1.169 148 8e-34 C2X1Y5.1 C2X1Y5_BACCE 2-dehydropantoate 2-reductase 115 7e-24 C2UZF8.1 C2UZF8_BACCE 2-dehydropantoate 2-reductase 115 7e-24 >B3KG70.1 B3KG70_9BACI 2-dehydropantoate 2-reductase EC=1.1.1.169 Length = 319 Score = 278 bits (710), Expect = 7e-73, Method: Compositional matrix adjust. Identities = 144/295 (48%), Positives = 183/295 (62%), Gaps = 1/295 (0%) Query: 26 VRLWCRSDEQAEELSRQGITISYEDGSLPLHLTGSSFRAGNIDKFAETVIRVPGDWTAIM 85 V +WCRS+EQA +L GITI +G P + +F + FAE+ D+ +M Sbjct: 26 VTVWCRSEEQASKLRTTGITIEKTEGK-PQTVAPHAFSVRAWNAFAESGAAADADYLFLM 84 Query: 86 LKQNAFHDTLPKILAPLRDARLYAVCFQNGNGHLELLQELLPQASVWVAVTTEAAKRKSL 145 LKQ + ++LAP R +CFQNG GHLE L+ELLP ++ A+TTE AKR S Sbjct: 85 LKQQGIEEMAREVLAPFGHHRRNLICFQNGTGHLERLRELLPMWKLYAAITTEGAKRTSS 144 Query: 146 TEIIHAGTGETFIGKRRSINPAKGGLEEETAAISLTKALVAAGFRALMSKEVDTMIYRKL 205 + ++HAG G T IGK G E I L K L AGF AL+S E+D MIYRKL Sbjct: 145 SSVLHAGHGTTTIGKMMPAASFIGATSHEDHDIELVKELNRAGFEALLSNEMDEMIYRKL 204 Query: 206 LFNAVINPLTAIWRIQNGELTASGERIQLMKELYREAASVYDACGIAYDADAWEGILEVC 265 L NAVINPLTA+WRI NG L +S ER LMK+LY E VY+A GI Y WE I+ VC Sbjct: 205 LMNAVINPLTALWRIPNGALLSSPERAGLMKQLYHEGILVYEAAGIPYGNKLWEWIVSVC 264 Query: 266 RATSGNTSSMLADVLASRATEIRWINGSIVAMADRAGIAAPLHRWICRLVEGMNV 320 ++TSGNTSSML DV+ R TE+ WINGSIV + + G++ P H I +L+EGMN+ Sbjct: 265 QSTSGNTSSMLKDVMDGRTTEVAWINGSIVQLGHKYGVSVPKHELITQLIEGMNL 319 >B1DBW5.1 B1DBW5_9BACL 2-dehydropantoate 2-reductase EC=1.1.1.169 Length = 290 Score = 169 bits (429), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 110/303 (36%), Positives = 159/303 (52%), Gaps = 24/303 (7%) Query: 28 LWCRSDEQAEELSRQGITISYEDG----------SLPLHLTGSSFRAGNIDKFAETVIRV 77 +W R+DEQA+ L +GI + DG SLP L G +AG AE Sbjct: 1 MWTRTDEQAKRLQAEGIRLEMLDGVARDIAVNSHSLPSLLAG---QAGQAGADAEHAAA- 56 Query: 78 PGDWTAIMLKQNAFHDTLPKILAPLRDARLYAVCFQNGNGHLELLQELLPQASVWVAVTT 137 W + +KQ + L L L + +C QNG GH++ L LP ++ VT+ Sbjct: 57 ---WIILTVKQCHIDERLLHQLQRLSSGKSALLCLQNGIGHMDKLAAALPGTLLYAGVTS 113 Query: 138 EAAKR-KSLTEIIHAGTGETFIGKRRSINPAKGGLEEETAAISLTKALVAAGFRALMSKE 196 A+R + H G G + G +++ L L +AGFRA +S E Sbjct: 114 AGARRIDDARHVRHTGDGPLWFGAYAEDK------KDDNLQNLLLNILKSAGFRAGLSNE 167 Query: 197 VDTMIYRKLLFNAVINPLTAIWRIQNGELTASGERIQLMKELYREAASVYDACGIAYDAD 256 + IY+KLL NAVINPLTAI+ NGEL R++LM+ L+ E +++ A G+ AD Sbjct: 168 MKDRIYQKLLINAVINPLTAIYDTTNGELPEHPSRLKLMRSLFEETSAILAADGMKSAAD 227 Query: 257 AWEGILEVCRATSGNTSSMLADVLASRATEIRWINGSIVAMADRAGIAAPLHRWICRLVE 316 W+ +++VC AT+ N SSML+DV A R TEI WING + +A R GIA+PL+ + LV+ Sbjct: 228 GWQLVMQVCAATATNVSSMLSDVRAGRHTEIDWINGGVSGLARRIGIASPLNDAVTTLVK 287 Query: 317 GMN 319 ++ Sbjct: 288 QLH 290 >C0ZGC2.1 C0ZGC2_BREBN 2-dehydropantoate 2-reductase EC=1.1.1.169 Length = 316 Score = 148 bits (373), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 96/293 (32%), Positives = 152/293 (51%), Gaps = 23/293 (7%) Query: 26 VRLWCRSDEQAEELSRQGITISYEDG-SLPLHLTGSSFRAGNIDKFAETVIRVP-GDWTA 83 V + RS QA +L+ +G++ + ++ + +T S G +P G+ Sbjct: 28 VTIVTRSSLQANQLNERGLSFQKLNSETVTVSVTASPIEEG-----------LPEGNLYL 76 Query: 84 IMLKQNAFHDTLPKILAPLRDARLYAVCFQNGNGHLELLQELLPQASVWVAVTTEAAKRK 143 + +KQ H LP + D + + QNG GH ELL+ +L + + A+ TE A+R Sbjct: 77 LTVKQPDLHSVLPALRG--LDLKARVIALQNGMGHYELLRTVLTETQCFFAINTEGARRL 134 Query: 144 SLTEIIHAGTGETFIGKRRSINPAKGGLEEETAAISLTKALVAAGFRALMSKEVDTMIYR 203 S TE++H GTG +G + + + + + ++ G A+ K + +R Sbjct: 135 SPTEVVHTGTGTLRVGSWETSDC------NDPLIRTFVEWAISTGMEAVYEKAIQPFAWR 188 Query: 204 KLLFNAVINPLTAIWRIQNGELTASGERIQLMKELYREAASVYDACGIAY-DADAWEGIL 262 KL+ NA+INPLTA++ I NG L + QLM+ L+ EAA+V G DAD E I+ Sbjct: 189 KLIANALINPLTALFEIPNGALLENSHTQQLMRALFVEAATVAAYAGQKIEDADLQE-IV 247 Query: 263 EVCRATSGNTSSMLADVLASRATEIRWINGSIVAMADRAGIAAPLHRWICRLV 315 +CR TS N SSML D+ R TE++ ING +V + +AGI PLH + R++ Sbjct: 248 SICRNTSRNLSSMLQDIKKRRPTEVQSINGYLVQVGQKAGIPTPLHETLLRVI 300 >C2X1Y5.1 C2X1Y5_BACCE 2-dehydropantoate 2-reductase Length = 296 Score = 115 bits (287), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 92/307 (29%), Positives = 152/307 (49%), Gaps = 46/307 (14%) Query: 25 EVRLWCRSDEQAEELSRQGITISYEDGS--------LPLHLTGSSFRAGNIDKFAETVIR 76 +V L+ R+ +QAEEL++ G+T DG LP+ E ++ Sbjct: 27 DVTLFTRTAKQAEELNKTGVT-CIRDGKRETVFPSILPI----------------EYMLD 69 Query: 77 VPGDWTAIMLKQNAFHDTLPKILAPLRDARLYAVCFQNGNGHLELLQELLPQASVWVAVT 136 D+T I +KQ +D LP + ++ L + QNG HL +Q++ + ++ V + Sbjct: 70 QKLDYTFIAVKQYHINDILPFVQG---ESSL--IFLQNGMSHLHAIQKIRNE-NIAVGIV 123 Query: 137 TEAAKRKSLTEIIHAGTGETFIGKRRSINPAKGGLEEETAAISLTKALVAAGFRALMSKE 196 AK++ + H G G T G R P+ L E + A F + + Sbjct: 124 EHGAKKEERYTVHHTGIGITKFGVVRG--PS---LYFE----KVFNYFPFAHFPIQIEAD 174 Query: 197 VDTMIYRKLLFNAVINPLTAIWRIQNGELTASGERIQLMKELYREAASVYDACGIAYDAD 256 ++Y+KL+ N INPLTA+ R+QNGEL + Q+M++++RE A + ++ + + Sbjct: 175 WKGIMYKKLVVNVCINPLTALLRVQNGELITNPFFYQMMEQVFREVAFL-----VSEEQE 229 Query: 257 -AWEGILEVCRATSGNTSSMLADVLASRATEIRWINGSIVAMADRAGIAAPLHRWICRLV 315 WE + +C TS NTSSMLADV A+R TEI I G ++ A + P +++ + Sbjct: 230 MVWEMVRGICERTSHNTSSMLADVRANRQTEIDAIVGYVLEEAKKQQRPVPTLQFLFDAI 289 Query: 316 EGMNVKE 322 +G+ VKE Sbjct: 290 KGLEVKE 296 >C2UZF8.1 C2UZF8_BACCE 2-dehydropantoate 2-reductase Length = 296 Score = 115 bits (287), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 90/310 (29%), Positives = 150/310 (48%), Gaps = 52/310 (16%) Query: 25 EVRLWCRSDEQAEELSRQGITISYEDGS--------LPLHLTGSSFRAGNIDKFAETVIR 76 +V L+ R+ +QAEEL++ G+T DG LP+ E ++ Sbjct: 27 DVTLFTRTAKQAEELNKTGVT-CIRDGKRETVFPSILPI----------------EYMLD 69 Query: 77 VPGDWTAIMLKQNAFHDTLPKILAPLRDARLYAVCFQNGNGHLELLQELLPQASVWVAVT 136 D+T I +KQ +D LP + ++ L + QNG HL +Q++ + ++ V + Sbjct: 70 QKLDYTFIAVKQYHINDILPFVQG---ESSL--IFLQNGMSHLHAIQKIRNE-NIAVGIV 123 Query: 137 TEAAKRKSLTEIIHAGTGETFIGKRRSINPAKGGLEEETAAISLTKAL---VAAGFRALM 193 AK++ + H G G T G R ++ K A F + Sbjct: 124 EHGAKKEERYTVHHTGIGITKFGVVRG------------PSLYFKKVFDYFPFAHFPIQI 171 Query: 194 SKEVDTMIYRKLLFNAVINPLTAIWRIQNGELTASGERIQLMKELYREAASVYDACGIAY 253 + ++Y+KL+ N INPLTA+ R+QNGEL + Q+M++++RE A + ++ Sbjct: 172 EADWKGIMYKKLVVNVCINPLTALLRVQNGELITNPFFYQMMEQVFREVAFL-----VSE 226 Query: 254 DAD-AWEGILEVCRATSGNTSSMLADVLASRATEIRWINGSIVAMADRAGIAAPLHRWIC 312 + + WE + +C TS NTSSMLADV A+R TEI I G ++ A + P +++ Sbjct: 227 EQEMVWEMVRGICERTSHNTSSMLADVRANRQTEIDAIVGYVLEEAKKQQRPVPTLQFLF 286 Query: 313 RLVEGMNVKE 322 ++G+ VKE Sbjct: 287 DAIKGLEVKE 296 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,705,602,042 Number of extensions: 65572297 Number of successful extensions: 170516 Number of sequences better than 1.0e-05: 748 Number of HSP's gapped: 169860 Number of HSP's successfully gapped: 748 Length of query: 323 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 187 Effective length of database: 1,779,438,163 Effective search space: 332754936481 Effective search space used: 332754936481 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)