BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0060.p (329 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG74.1 B3KG74_9BACI Binding-protein-dependent transport system... 428 e-118 B1DEI4.1 B1DEI4_9BACL Binding-protein-dependent transport system... 328 3e-88 Q2B8Z2.1 Q2B8Z2_9BACI OppB 313 1e-83 A7Z3D2.1 A7Z3D2_BACA2 OppB 308 4e-82 P24138.2 OPPB_BACSU Oligopeptide transport system permease prote... 306 1e-81 >B3KG74.1 B3KG74_9BACI Binding-protein-dependent transport systems inner membrane component Length = 310 Score = 428 bits (1101), Expect = e-118, Method: Compositional matrix adjust. Identities = 214/309 (69%), Positives = 245/309 (79%) Query: 20 MVRYVANKFFYMLVSLFVLVSATFFLMKAIPGDPFTSEKKVPPEIKARLYEQYGLDKPLY 79 M+RYVA+KFFYMLVSLF+L+SATFFLMKAIPGDPF EKK PEI ARL EQY LDKP++ Sbjct: 1 MLRYVASKFFYMLVSLFILISATFFLMKAIPGDPFMGEKKPSPEILARLMEQYDLDKPVF 60 Query: 80 HQYFKYLGEIAQGDLGVSMKRLNQDVTHLIGQTFSASLKLXXXXXXXXXXXXXXXXMLAA 139 QY KYLG I QGD G+SMK+ Q VT +I TF SL+L MLAA Sbjct: 61 QQYTKYLGNIIQGDFGLSMKKQGQSVTDIIVDTFGPSLRLGLIAIVVSVIVGVLLGMLAA 120 Query: 140 LYHRKFIDSAAMVLAVLGIAVPSFVVASLLQYVFAYKLHLFPVSGFKGPMYYFLPVAALS 199 LYHRK ID+ AM+++VLGIAVPSFV+ASLLQ+V A K +F V GF+GP+ Y LPV ALS Sbjct: 121 LYHRKLIDNIAMIISVLGIAVPSFVLASLLQFVLAEKAGIFNVMGFEGPLDYVLPVMALS 180 Query: 200 AQPIAFIARLTRSSMLEVLHADYIKTAKAKGLSWFTILSRHVLRNGILPIVTYMGPMTAN 259 AQPIAFIARLTRSSMLEVLH+DYIKTAKAKGL+WF I+ RHV+RNGILP+VTY+GPMTAN Sbjct: 181 AQPIAFIARLTRSSMLEVLHSDYIKTAKAKGLNWFAIMFRHVIRNGILPVVTYVGPMTAN 240 Query: 260 IVTGSVVXXXXXXXXXXXKQFVEAIGVRDYTVIMGITIFYGILLMVARFITDIAYVLIDP 319 I+TGSVV KQFVE+I RDYTVIMGITIFYGILLM+ARF+TDI YV +DP Sbjct: 241 IITGSVVIEQIFGIGGIGKQFVESITNRDYTVIMGITIFYGILLMLARFVTDIVYVFVDP 300 Query: 320 RIKLSGGKE 328 RIKL+GGKE Sbjct: 301 RIKLTGGKE 309 >B1DEI4.1 B1DEI4_9BACL Binding-protein-dependent transport systems inner membrane component Length = 310 Score = 328 bits (842), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 171/310 (55%), Positives = 212/310 (68%) Query: 20 MVRYVANKFFYMLVSLFVLVSATFFLMKAIPGDPFTSEKKVPPEIKARLYEQYGLDKPLY 79 MVRYV K +M+VS+FVL SATFFLMKAIPG+PF SEK +PP I+ RL E YGL+ PL Sbjct: 1 MVRYVLQKLLFMIVSIFVLASATFFLMKAIPGNPFQSEKNIPPAIQERLNEAYGLNDPLG 60 Query: 80 HQYFKYLGEIAQGDLGVSMKRLNQDVTHLIGQTFSASLKLXXXXXXXXXXXXXXXXMLAA 139 QY YL + +LG+SMK+ V +I F SL++ ++AA Sbjct: 61 VQYLHYLNNLIHFNLGMSMKQQYVTVVDIIKTGFIYSLQIGVFAIILAIAAGVLFGLIAA 120 Query: 140 LYHRKFIDSAAMVLAVLGIAVPSFVVASLLQYVFAYKLHLFPVSGFKGPMYYFLPVAALS 199 L HRKF+D ++LAVLG+++PSFVVA LQYVF KL F V+G P + +PV ALS Sbjct: 121 LNHRKFLDGFTILLAVLGVSIPSFVVAPFLQYVFGVKLRWFDVAGLNDPTDFIMPVIALS 180 Query: 200 AQPIAFIARLTRSSMLEVLHADYIKTAKAKGLSWFTILSRHVLRNGILPIVTYMGPMTAN 259 P AFIARL RSSMLEVL ADY+KTA+AKGLS IL RH LRN ILP+VTY+GPMTAN Sbjct: 181 FLPAAFIARLVRSSMLEVLSADYMKTARAKGLSGRVILVRHALRNAILPVVTYIGPMTAN 240 Query: 260 IVTGSVVXXXXXXXXXXXKQFVEAIGVRDYTVIMGITIFYGILLMVARFITDIAYVLIDP 319 I+TGSVV K FV +I RDYT+IMG+T+FY ++LM+ARF+TDIAY +DP Sbjct: 241 IITGSVVIEQIFGIGGLGKFFVNSITNRDYTLIMGLTLFYAVILMLARFLTDIAYGFVDP 300 Query: 320 RIKLSGGKEG 329 RIK++ K G Sbjct: 301 RIKMTSRKGG 310 >Q2B8Z2.1 Q2B8Z2_9BACI OppB Length = 311 Score = 313 bits (803), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 159/310 (51%), Positives = 213/310 (68%) Query: 20 MVRYVANKFFYMLVSLFVLVSATFFLMKAIPGDPFTSEKKVPPEIKARLYEQYGLDKPLY 79 M +Y+ + YM+VSL+++++ATFF M+ PG+PFTSEK++PPEI+A L YGLD+P Y Sbjct: 1 MAKYIGKRLIYMIVSLWLIITATFFFMRIAPGNPFTSEKQLPPEIEANLNAHYGLDQPWY 60 Query: 80 HQYFKYLGEIAQGDLGVSMKRLNQDVTHLIGQTFSASLKLXXXXXXXXXXXXXXXXMLAA 139 QY +YL I + D G S K +Q V LI + F SL L ++AA Sbjct: 61 VQYGEYLLRIIKWDFGPSFKYKSQTVNDLISEGFPVSLMLGIEAILIAVSIGVVLGVIAA 120 Query: 140 LYHRKFIDSAAMVLAVLGIAVPSFVVASLLQYVFAYKLHLFPVSGFKGPMYYFLPVAALS 199 L H K+ D AM++AV+GI+VPSF++A+ LQY A KL +FPV+ ++ M+ LP AL+ Sbjct: 121 LKHNKWQDYTAMIVAVMGISVPSFIMATFLQYFLAIKLGVFPVARWESFMHTILPALALA 180 Query: 200 AQPIAFIARLTRSSMLEVLHADYIKTAKAKGLSWFTILSRHVLRNGILPIVTYMGPMTAN 259 A P+AFIARLTRSSMLEVL DYIKTAK+KGLS I +H +RN +LP+V+YMGP++A Sbjct: 181 ASPMAFIARLTRSSMLEVLSNDYIKTAKSKGLSQGVITIKHTIRNAMLPVVSYMGPLSAG 240 Query: 260 IVTGSVVXXXXXXXXXXXKQFVEAIGVRDYTVIMGITIFYGILLMVARFITDIAYVLIDP 319 I+TGS V FV +IG RDYTVIMG+T+FY I+L+V+ + DIAY LIDP Sbjct: 241 IITGSFVIEKIFGIPGLGSHFVLSIGNRDYTVIMGVTVFYSIILLVSILLVDIAYGLIDP 300 Query: 320 RIKLSGGKEG 329 RIKL+GGK+G Sbjct: 301 RIKLAGGKKG 310 >A7Z3D2.1 A7Z3D2_BACA2 OppB Length = 311 Score = 308 bits (790), Expect = 4e-82, Method: Compositional matrix adjust. Identities = 158/310 (50%), Positives = 212/310 (68%) Query: 20 MVRYVANKFFYMLVSLFVLVSATFFLMKAIPGDPFTSEKKVPPEIKARLYEQYGLDKPLY 79 M++Y+ +F YM+++LFV+V+ATFFLM+A PG PF+ E+K+PPEI+A L E YGLDKPL+ Sbjct: 1 MLKYIGKRFIYMIITLFVIVTATFFLMQAAPGGPFSGERKLPPEIEANLNEHYGLDKPLF 60 Query: 80 HQYFKYLGEIAQGDLGVSMKRLNQDVTHLIGQTFSASLKLXXXXXXXXXXXXXXXXMLAA 139 QY YL +A D G S K Q V LI F S L +LAA Sbjct: 61 VQYVSYLKSVAMWDFGPSFKYKGQSVNDLINSGFPVSFTLGAEAILLALAFGVLFGVLAA 120 Query: 140 LYHRKFIDSAAMVLAVLGIAVPSFVVASLLQYVFAYKLHLFPVSGFKGPMYYFLPVAALS 199 LYH K+ D +L + GI+VPSF++A++LQY+F+ KL LFPV+G++ Y FLP AL+ Sbjct: 121 LYHNKWQDYTIAILTIFGISVPSFILATILQYIFSIKLELFPVAGWESWAYTFLPSIALA 180 Query: 200 AQPIAFIARLTRSSMLEVLHADYIKTAKAKGLSWFTILSRHVLRNGILPIVTYMGPMTAN 259 + P+AFIARL+RSSM+EVL++DYI+TAKAKGLS ++ RH LRN +LP+VTYMGPM A+ Sbjct: 181 SMPMAFIARLSRSSMIEVLNSDYIRTAKAKGLSRPSVTVRHALRNALLPVVTYMGPMAAS 240 Query: 260 IVTGSVVXXXXXXXXXXXKQFVEAIGVRDYTVIMGITIFYGILLMVARFITDIAYVLIDP 319 I+TGS + FV +I RDYTVIMG+T+F+ +LL++ I D+ Y LIDP Sbjct: 241 ILTGSFIIESIFGIPGLGAHFVNSITNRDYTVIMGVTVFFSVLLLLCVLIVDVLYGLIDP 300 Query: 320 RIKLSGGKEG 329 RIKLS K+G Sbjct: 301 RIKLSKAKKG 310 >P24138.2 OPPB_BACSU Oligopeptide transport system permease protein oppB Length = 311 Score = 306 bits (785), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 152/310 (49%), Positives = 206/310 (66%) Query: 20 MVRYVANKFFYMLVSLFVLVSATFFLMKAIPGDPFTSEKKVPPEIKARLYEQYGLDKPLY 79 M++Y+ + YM+++LFV+V+ TFFLM+A PG PF+ EKK+PPEI+A L YGLDKPL+ Sbjct: 1 MLKYIGRRLVYMIITLFVIVTVTFFLMQAAPGGPFSGEKKLPPEIEANLNAHYGLDKPLF 60 Query: 80 HQYFKYLGEIAQGDLGVSMKRLNQDVTHLIGQTFSASLKLXXXXXXXXXXXXXXXXMLAA 139 QY YL +A D G S K Q V LI F S L ++AA Sbjct: 61 VQYVSYLKSVAMWDFGPSFKYKGQSVNDLISSGFPVSFTLGAEAILLALALGVLFGVIAA 120 Query: 140 LYHRKFIDSAAMVLAVLGIAVPSFVVASLLQYVFAYKLHLFPVSGFKGPMYYFLPVAALS 199 LYH K+ D +L + GI+VPSF++A++LQYVF+ KL LFPV+G+ Y FLP AL+ Sbjct: 121 LYHNKWQDYTVAILTIFGISVPSFIMAAVLQYVFSMKLGLFPVAGWDSWAYTFLPSIALA 180 Query: 200 AQPIAFIARLTRSSMLEVLHADYIKTAKAKGLSWFTILSRHVLRNGILPIVTYMGPMTAN 259 + P+AFIARL+RSSM+EVL++DYI+TAKAKGLS + RH +RN +LP+VTYMGPM A Sbjct: 181 SMPMAFIARLSRSSMIEVLNSDYIRTAKAKGLSRPAVTVRHAIRNALLPVVTYMGPMAAQ 240 Query: 260 IVTGSVVXXXXXXXXXXXKQFVEAIGVRDYTVIMGITIFYGILLMVARFITDIAYVLIDP 319 ++TGS + FV +I RDYTVIMG+T+F+ ++L++ I D+ Y +IDP Sbjct: 241 VLTGSFIIETIFGIPGLGAHFVNSITNRDYTVIMGVTVFFSVILLLCVLIVDVLYGIIDP 300 Query: 320 RIKLSGGKEG 329 RIKLS K+G Sbjct: 301 RIKLSKAKKG 310 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.143 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,641,927,161 Number of extensions: 59069416 Number of successful extensions: 214039 Number of sequences better than 1.0e-05: 5900 Number of HSP's gapped: 200305 Number of HSP's successfully gapped: 6218 Length of query: 329 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 193 Effective length of database: 1,779,438,163 Effective search space: 343431565459 Effective search space used: 343431565459 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)