BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0053.p (311 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG81.1 B3KG81_9BACI S-adenosyl-methyltransferase MraW 514 e-144 B1DBX3.1 B1DBX3_9BACL S-adenosyl-methyltransferase MraW 467 e-130 C0ZGB3.1 C0ZGB3_BREBN S-adenosyl-methyltransferase EC=2.1.1.- 440 e-122 C2WB53.1 C2WB53_BACCE S-adenosyl-L-methionine-dependent methyltr... 387 e-106 B1HPY0.1 B1HPY0_LYSSC S-adenosyl-L-methionine-dependent methyltr... 384 e-105 >B3KG81.1 B3KG81_9BACI S-adenosyl-methyltransferase MraW Length = 317 Score = 514 bits (1325), Expect = e-144, Method: Compositional matrix adjust. Identities = 247/312 (79%), Positives = 277/312 (88%), Gaps = 2/312 (0%) Query: 1 VLKEEATEGLHIKKDGIYVDCTLGGAGHSSLIAGRLSGEGRLICLDQDDWALENARERLA 60 VLK+EATEGL IK DGIYVDCTLGGAGHSSLIA +LS EGRLI LDQDDWAL NARE + Sbjct: 7 VLKQEATEGLRIKPDGIYVDCTLGGAGHSSLIASQLSSEGRLIALDQDDWALNNAREAMK 66 Query: 61 EYGEKVVLVKTNFRDLEQVLKDLPFVPQKDGIPQVDGILYDLGVSSPQFDEGERGFSYNH 120 Y +++ LVKTNFRDLE+VL+ ++G PQVDGIL+DLGVSSPQFDEGERGFSYNH Sbjct: 67 PYADRISLVKTNFRDLEEVLRR-EVGAGENGQPQVDGILFDLGVSSPQFDEGERGFSYNH 125 Query: 121 DAPLDMRMDQSAQLTAAEIVNTWPEQEIARVLFQYGEEKFSRRIARKIVERREEHPVEST 180 DAPLDMRMDQSA+LTAA+IVN WPEQEIAR+L+QYGEEKFSRRIA+ IVERR + P+++T Sbjct: 126 DAPLDMRMDQSAELTAAKIVNEWPEQEIARILYQYGEEKFSRRIAKVIVERRAQSPIQTT 185 Query: 181 GELAELIKEGIPAAARRTGGHPAKRSFQGLRIAVNDELGAFEEGLHAAVRCLAPGGRVSV 240 GEL E+IKEGIPAAARRTGGHPAKRSFQ RIAVNDELGAFE+ LH AVRCLAPGGR+SV Sbjct: 186 GELVEMIKEGIPAAARRTGGHPAKRSFQAFRIAVNDELGAFEDALHQAVRCLAPGGRISV 245 Query: 241 ITFHSLEDRICKQIFSSYLSRCTCPPDFPFCVC-GAEGTLKLINRKPLVPSEEELELNPR 299 ITFHSLEDRICKQIF+SY+ +CTCPPDFP CVC G +G LKL+NRKPL+PS EE+E NPR Sbjct: 246 ITFHSLEDRICKQIFNSYIPKCTCPPDFPMCVCGGGQGDLKLVNRKPLIPSAEEIESNPR 305 Query: 300 ARSAKLRIAEKL 311 ARSAKLRIAEKL Sbjct: 306 ARSAKLRIAEKL 317 >B1DBX3.1 B1DBX3_9BACL S-adenosyl-methyltransferase MraW Length = 316 Score = 467 bits (1202), Expect = e-130, Method: Compositional matrix adjust. Identities = 225/311 (72%), Positives = 261/311 (83%), Gaps = 1/311 (0%) Query: 1 VLKEEATEGLHIKKDGIYVDCTLGGAGHSSLIAGRLSGEGRLICLDQDDWALENARERLA 60 VL EEA +GL IK GIYVDCTLGGAGHS I +LSG GRLI DQDD AL+NAR RLA Sbjct: 7 VLLEEAVDGLAIKPGGIYVDCTLGGAGHSERIVSQLSGGGRLIAFDQDDAALDNARVRLA 66 Query: 61 EYGEKVVLVKTNFRDLEQVLKDLPFVPQKDGIPQVDGILYDLGVSSPQFDEGERGFSYNH 120 Y ++V LVK+NFR LE++L VP DG+PQVDGIL+DLGVSSPQ DE ERGFSYNH Sbjct: 67 PYMDRVTLVKSNFRYLEEMLLTCD-VPTVDGVPQVDGILFDLGVSSPQLDEAERGFSYNH 125 Query: 121 DAPLDMRMDQSAQLTAAEIVNTWPEQEIARVLFQYGEEKFSRRIARKIVERREEHPVEST 180 DAPLDMRMDQ LTA +IVN+W E+EI+R+L YGEEKF+R IARKIV+ RE P+E+T Sbjct: 126 DAPLDMRMDQGGALTAYDIVNSWEEREISRILHVYGEEKFARSIARKIVQARENAPIETT 185 Query: 181 GELAELIKEGIPAAARRTGGHPAKRSFQGLRIAVNDELGAFEEGLHAAVRCLAPGGRVSV 240 GELAEL+K GIPAAARRTGGHPAKRSFQ LRIAVNDELGA E+ L AV+C+ PGGR+SV Sbjct: 186 GELAELVKTGIPAAARRTGGHPAKRSFQALRIAVNDELGAEEDALEEAVKCIRPGGRISV 245 Query: 241 ITFHSLEDRICKQIFSSYLSRCTCPPDFPFCVCGAEGTLKLINRKPLVPSEEELELNPRA 300 ITFHSLEDRICKQ+F+SY+ +CTCPPDFP CVCG G L+L+NRKP+VP+E+ELE+NPR+ Sbjct: 246 ITFHSLEDRICKQLFASYVEKCTCPPDFPKCVCGGTGKLRLVNRKPIVPTEQELEVNPRS 305 Query: 301 RSAKLRIAEKL 311 RSAKLR+AEKL Sbjct: 306 RSAKLRVAEKL 316 >C0ZGB3.1 C0ZGB3_BREBN S-adenosyl-methyltransferase EC=2.1.1.- Length = 314 Score = 440 bits (1132), Expect = e-122, Method: Compositional matrix adjust. Identities = 208/311 (66%), Positives = 255/311 (81%), Gaps = 7/311 (2%) Query: 1 VLKEEATEGLHIKKDGIYVDCTLGGAGHSSLIAGRLSGEGRLICLDQDDWALENARERLA 60 VL +EA +GL+I+ GIYVDCTLGGAGHSSLIA +L+ GRLI +DQDDWAL+NARERLA Sbjct: 11 VLMDEAVQGLNIRPGGIYVDCTLGGAGHSSLIASKLTEGGRLIAIDQDDWALDNARERLA 70 Query: 61 EYGEKVVLVKTNFRDLEQVLKDLPFVPQKDGIPQVDGILYDLGVSSPQFDEGERGFSYNH 120 Y ++V LVK+NFR ++ ++KDL G+ VDG+L+DLGVSSPQ DEGERGFSYN Sbjct: 71 SYMDRVTLVKSNFRHIKDIVKDL-------GLSGVDGVLFDLGVSSPQLDEGERGFSYNA 123 Query: 121 DAPLDMRMDQSAQLTAAEIVNTWPEQEIARVLFQYGEEKFSRRIARKIVERREEHPVEST 180 DAPLDMRMDQ A L+A +I+N W E+EIA++++ YGEEKFSRRIAR+IV++R++ P+++T Sbjct: 124 DAPLDMRMDQQAPLSAYDIINEWDEEEIAKIIWLYGEEKFSRRIARQIVQQRKKQPIQTT 183 Query: 181 GELAELIKEGIPAAARRTGGHPAKRSFQGLRIAVNDELGAFEEGLHAAVRCLAPGGRVSV 240 GEL ELIKEGIPAAARRTG HPAKR+FQ +RIAVNDEL AF+E + A+ L P GRVSV Sbjct: 184 GELVELIKEGIPAAARRTGPHPAKRTFQAIRIAVNDELDAFKEAVVDAIEVLNPEGRVSV 243 Query: 241 ITFHSLEDRICKQIFSSYLSRCTCPPDFPFCVCGAEGTLKLINRKPLVPSEEELELNPRA 300 ITFHSLEDRICKQI+ + CTCPP FP C CG + +K+I RKP++PSEEELE N RA Sbjct: 244 ITFHSLEDRICKQIYQDFSKGCTCPPAFPICTCGNKAVVKVITRKPILPSEEELEANKRA 303 Query: 301 RSAKLRIAEKL 311 RSAKLR+AEKL Sbjct: 304 RSAKLRVAEKL 314 >C2WB53.1 C2WB53_BACCE S-adenosyl-L-methionine-dependent methyltransferase mraW Length = 332 Score = 387 bits (995), Expect = e-106, Method: Compositional matrix adjust. Identities = 192/310 (61%), Positives = 229/310 (73%), Gaps = 7/310 (2%) Query: 1 VLKEEATEGLHIKKDGIYVDCTLGGAGHSSLIAGRLSGEGRLICLDQDDWALENARERLA 60 VL +E +GL IK G YVDCTLGG GHSS + RL+ G+LI DQD+ A++NA+E+ + Sbjct: 29 VLLKETVDGLDIKPGGTYVDCTLGGGGHSSYLLSRLTEGGKLIAFDQDEIAIQNAKEKFS 88 Query: 61 EYGEKVVLVKTNFRDLEQVLKDLPFVPQKDGIPQVDGILYDLGVSSPQFDEGERGFSYNH 120 YG++ V VK+NFR L + L++L GI +VDGIL+DLGVSSPQ D ERGFSY+H Sbjct: 89 SYGDQFVTVKSNFRYLSEKLQEL-------GITEVDGILFDLGVSSPQLDTPERGFSYHH 141 Query: 121 DAPLDMRMDQSAQLTAAEIVNTWPEQEIARVLFQYGEEKFSRRIARKIVERREEHPVEST 180 DAPLDMRMDQ A LTA E+VN W +++ R+ FQYGEEKFS++IARKI RE +E+T Sbjct: 142 DAPLDMRMDQDASLTAYEVVNNWSYEQLVRIFFQYGEEKFSKQIARKIEAYRENKTIETT 201 Query: 181 GELAELIKEGIPAAARRTGGHPAKRSFQGLRIAVNDELGAFEEGLHAAVRCLAPGGRVSV 240 GEL ELIKEGIPA ARRTGGHPAKR FQ +RIAVNDEL FEE L +A++ + PGGRVSV Sbjct: 202 GELVELIKEGIPAPARRTGGHPAKRVFQAIRIAVNDELKVFEEALESAIKMVKPGGRVSV 261 Query: 241 ITFHSLEDRICKQIFSSYLSRCTCPPDFPFCVCGAEGTLKLINRKPLVPSEEELELNPRA 300 ITFHSLEDRICK F S PP P + LKLI RKP++PSEEELE N RA Sbjct: 262 ITFHSLEDRICKTTFKRNSSTPQLPPGLPIIPDEFKPKLKLITRKPILPSEEELEENNRA 321 Query: 301 RSAKLRIAEK 310 RSAKLRIAEK Sbjct: 322 RSAKLRIAEK 331 >B1HPY0.1 B1HPY0_LYSSC S-adenosyl-L-methionine-dependent methyltransferase mraW EC=2.1.1.- Length = 316 Score = 384 bits (986), Expect = e-105, Method: Compositional matrix adjust. Identities = 190/311 (61%), Positives = 232/311 (74%), Gaps = 7/311 (2%) Query: 1 VLKEEATEGLHIKKDGIYVDCTLGGAGHSSLIAGRLSGEGRLICLDQDDWALENARERLA 60 VL +E +GL+I DG+YVDCTLGGAGHS + +LS +GRLIC DQD A+ENA+ RLA Sbjct: 7 VLLKETVDGLNIDPDGVYVDCTLGGAGHSEYLVQQLSDKGRLICFDQDMTAIENAKIRLA 66 Query: 61 EYGEKVVLVKTNFRDLEQVLKDLPFVPQKDGIPQVDGILYDLGVSSPQFDEGERGFSYNH 120 Y E+V + +NFR L++ L G QVDGILYDLGVSSPQ D ERGFSY+H Sbjct: 67 PYIERVTFIHSNFRYLKEEL-------LAHGFEQVDGILYDLGVSSPQLDTPERGFSYHH 119 Query: 121 DAPLDMRMDQSAQLTAAEIVNTWPEQEIARVLFQYGEEKFSRRIARKIVERREEHPVEST 180 DAPLDMRMDQ+A LTA E+VN W +++ R+ F+YGEEKFS+++ARKI E R+ P+E+T Sbjct: 120 DAPLDMRMDQTATLTAFEVVNQWAYEDLVRIFFRYGEEKFSKQVARKIEEARKSAPIETT 179 Query: 181 GELAELIKEGIPAAARRTGGHPAKRSFQGLRIAVNDELGAFEEGLHAAVRCLAPGGRVSV 240 G+L ELIKEGIPAAARR GGHPAKR FQ +RIAVNDELGA E+ L A+ + GGR+SV Sbjct: 180 GQLVELIKEGIPAAARRKGGHPAKRIFQAIRIAVNDELGAAEDSLVDAIDMINVGGRISV 239 Query: 241 ITFHSLEDRICKQIFSSYLSRCTCPPDFPFCVCGAEGTLKLINRKPLVPSEEELELNPRA 300 ITFHSLEDR+CK IF S PP+ P + TLKLI RKP+VPS+EELE+N RA Sbjct: 240 ITFHSLEDRLCKTIFKEASSLPELPPNLPVIPDDMKPTLKLITRKPIVPSDEELEVNNRA 299 Query: 301 RSAKLRIAEKL 311 RSAKLR+ EK+ Sbjct: 300 RSAKLRVVEKI 310 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,895,386,601 Number of extensions: 81070127 Number of successful extensions: 211389 Number of sequences better than 1.0e-05: 1607 Number of HSP's gapped: 206729 Number of HSP's successfully gapped: 1610 Length of query: 311 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 176 Effective length of database: 1,788,859,178 Effective search space: 314839215328 Effective search space used: 314839215328 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)