BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0050.p (269 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG83.1 B3KG83_9BACI Peptidoglycan glycosyltransferase EC=2.4.1... 245 3e-63 B1DBX5.1 B1DBX5_9BACL Peptidoglycan glycosyltransferase EC=2.4.1... 201 5e-50 B1DEN3.1 B1DEN3_9BACL Peptidoglycan glycosyltransferase EC=2.4.1... 201 7e-50 Q2RK85.1 Q2RK85_MOOTA Stage V sporulation protein D EC=2.4.1.129 142 4e-32 Q3AAE0.1 Q3AAE0_CARHZ Penicillin-binding protein 2B 130 1e-28 >B3KG83.1 B3KG83_9BACI Peptidoglycan glycosyltransferase EC=2.4.1.129 Length = 768 Score = 245 bits (626), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 137/253 (54%), Positives = 174/253 (68%), Gaps = 6/253 (2%) Query: 1 VVRQVISKESARETGSYLEQVVADQVKGTGRHAYIEGYRVAGKTGTAIKVRGTDYDRNKV 60 VVRQVIS E+ARETG+YLEQVVADQ+ GTG+ AYI+GYRVAGKTGTAIK G DR+K Sbjct: 471 VVRQVISPEAARETGTYLEQVVADQLNGTGKLAYIDGYRVAGKTGTAIKSGGGYKDRSKQ 530 Query: 61 LSSFIGYAPVNDPKIAVIVIIDEPKXXXXXXXXXAVAAPVFQEIVSQALPYMGVPKASTE 120 + SFIGYAPV +PKIAV+VIIDEP A+AAP+F+EIVS+ L Y GVPK +TE Sbjct: 531 VVSFIGYAPVENPKIAVLVIIDEPN---VEKGGGALAAPIFKEIVSKTLNYWGVPK-TTE 586 Query: 121 TAD-STSKTVKAPAPAQRSAPDLTGKTMQAARQLLIDQGFDFEAVGEGTTVVSQYPEKGT 179 T D S AP A + APD TG + + L+ G FE +G G+TV QYP + Sbjct: 587 TQDKEKSNPGTAPKDALK-APDFTGMKLSEVKAKLLKDGVAFETLGNGSTVKEQYPPANS 645 Query: 180 PLASGQRIYLLSQQGDKPVIPDLKGQSLRDALEVLSLLKVGIAVEGEGYVSEQAQGLKNG 239 + GQRIYLL++ IP+L+G+SLRDA+EVL+L+KV + V+GEG+V Q + +NG Sbjct: 646 IINVGQRIYLLTEDSPSMKIPNLEGESLRDAMEVLTLMKVKVNVQGEGFVVSQLEAKENG 705 Query: 240 KTLVTLKLNPVNE 252 + +V L L E Sbjct: 706 QRIVQLTLKSAQE 718 >B1DBX5.1 B1DBX5_9BACL Peptidoglycan glycosyltransferase EC=2.4.1.129 Length = 751 Score = 201 bits (512), Expect = 5e-50, Method: Compositional matrix adjust. Identities = 113/249 (45%), Positives = 155/249 (62%), Gaps = 9/249 (3%) Query: 1 VVRQVISKESARETGSYLEQVVADQVKGTGRHAYIEGYRVAGKTGTAIKVRGTDYDRNKV 60 VVRQVIS+E++++ G YLEQVV+DQ GTGR+AYIEGYRVAGKTGTA KV Y ++K Sbjct: 470 VVRQVISQEASKKVGEYLEQVVSDQEIGTGRNAYIEGYRVAGKTGTAQKVINGKYSQDKF 529 Query: 61 LSSFIGYAPVNDPKIAVIVIIDEPKXXXXXXXXXAVAAPVFQEIVSQALPYMGVPKASTE 120 + SFIGYAPV +PKI + +++DEP A A F+++V ++L +MG+ Sbjct: 530 VVSFIGYAPVENPKIVLYIVVDEPNDPKAGGGAVAAPA--FKDVVYKSLKHMGI------ 581 Query: 121 TADSTSKTVKAPAPAQRSAPDLTGKTMQAARQLLIDQGFDFEAVGEGTTVVSQYPEKGTP 180 A T S P L T+ A+ L +G + E VG GT V+ Q P G Sbjct: 582 -APDIKVTENGKKEVTVSVPKLASLTVSQAKTQLKSRGLNVEVVGGGTKVIQQVPASGAS 640 Query: 181 LASGQRIYLLSQQGDKPVIPDLKGQSLRDALEVLSLLKVGIAVEGEGYVSEQAQGLKNGK 240 + Q++YL+++Q DK IPD+ G SLRDALE+ S+L + + V+GEGYV Q Q KNG+ Sbjct: 641 VHPAQKVYLITEQRDKLKIPDMTGISLRDALEIASVLDIRVTVKGEGYVVSQKQVTKNGE 700 Query: 241 TLVTLKLNP 249 L+ L L+P Sbjct: 701 RLIELTLSP 709 >B1DEN3.1 B1DEN3_9BACL Peptidoglycan glycosyltransferase EC=2.4.1.129 Length = 711 Score = 201 bits (510), Expect = 7e-50, Method: Compositional matrix adjust. Identities = 114/250 (45%), Positives = 154/250 (61%), Gaps = 9/250 (3%) Query: 1 VVR-QVISKESARETGSYLEQVVADQVKGTGRHAYIEGYRVAGKTGTAIKVRGTDYDRNK 59 VVR VISKE++++ G YLEQVV+D GTG +A+IEGYRVAGKTGTA KV Y RNK Sbjct: 455 VVRSNVISKETSKKVGEYLEQVVSDHDLGTGHNAFIEGYRVAGKTGTAEKVINGKYSRNK 514 Query: 60 VLSSFIGYAPVNDPKIAVIVIIDEPKXXXXXXXXXAVAAPVFQEIVSQALPYMGVPKAST 119 + SFIGYAPV +PKI + +++DEP VAAP F++IV ++L +MGV Sbjct: 515 FVVSFIGYAPVENPKIVLYIVVDEPDDPTAGGGK--VAAPAFKDIVYKSLKHMGVSPQYK 572 Query: 120 ETADSTSKTVKAPAPAQRSAPDLTGKTMQAARQLLIDQGFDFEAVGEGTTVVSQYPEKGT 179 TA + V S P+L + A+ L +G + E VG G T++ Q P G Sbjct: 573 VTAGTAKNEVTV------SIPELAKLKVTEAKSQLRSRGLNAEIVGSGKTIIQQIPASGA 626 Query: 180 PLASGQRIYLLSQQGDKPVIPDLKGQSLRDALEVLSLLKVGIAVEGEGYVSEQAQGLKNG 239 + GQ++YL+++Q DK IPD+ G SLRDAL + S+L + + V GEGYV Q Q KNG Sbjct: 627 LVHPGQKVYLITEQRDKLQIPDMTGLSLRDALRIASVLDIQVTVSGEGYVVSQKQITKNG 686 Query: 240 KTLVTLKLNP 249 + + L L+P Sbjct: 687 ERQLQLTLSP 696 >Q2RK85.1 Q2RK85_MOOTA Stage V sporulation protein D EC=2.4.1.129 Length = 711 Score = 142 bits (357), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 95/234 (40%), Positives = 134/234 (57%), Gaps = 13/234 (5%) Query: 1 VVRQVISKESARETGSYLEQVVADQVKGTGRHAYIEGYRVAGKTGTAIKVRGTDYDRNKV 60 V RQVIS E+A++ LE VV++ GTGR+AYI GYRV GKTGTA K Y K Sbjct: 461 VERQVISPETAKQLREMLETVVSE---GTGRNAYIPGYRVGGKTGTAQKAGPGGYMEGKY 517 Query: 61 LSSFIGYAPVNDPKIAVIVIIDEPKXXXXXXXXXAVAAPVFQEIVSQALPYMGVPKASTE 120 ++SFIG APVNDP++ +V IDEPK +AAP+FQ +V+ AL Y+ VP Sbjct: 518 VASFIGMAPVNDPRLVALVTIDEPK--GYPYYGGTLAAPIFQRVVADALHYLKVPP---- 571 Query: 121 TADSTSKTVKAPAPAQRSAPDLTGKTMQAARQLLIDQGFDFEAVGEGTTVVSQYPEKGTP 180 DS + + PA A + P++TGK + AAR L G G+ V++Q P+ G Sbjct: 572 QYDSKQGSEEKPA-APVTVPNVTGKDLAAARAELEKAGLAARVEGDSGKVIAQVPKAGAV 630 Query: 181 LASGQRIYLLSQQGD--KPVIPDLKGQSLRDALEVLSLLKVGIAVEGEGYVSEQ 232 + +G ++ +L QGD +P +PD+ + +A EVL+ + + EG G EQ Sbjct: 631 VPAGTKV-ILYLQGDPNRPRVPDVNSLRVTEAAEVLAAYGLQLVPEGTGQAGEQ 683 >Q3AAE0.1 Q3AAE0_CARHZ Penicillin-binding protein 2B Length = 739 Score = 130 bits (327), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 92/237 (38%), Positives = 126/237 (53%), Gaps = 13/237 (5%) Query: 2 VRQVISKESARETGSYLEQVVADQVKGTGRHAYIEGYRVAGKTGTAIKVRGTD-YDRNKV 60 +++VIS+E+AR LE+VV +KGTG AYI+G+RVAGKTGTA V T Y K Sbjct: 490 LKRVISEETARTLRELLERVV---LKGTGTKAYIKGFRVAGKTGTAQVVSPTGGYAPGKY 546 Query: 61 LSSFIGYAPVNDPKIAVIVIIDEPKXXXXXXXXXAVAAPVFQEIVSQALPYMGV-PKAST 119 ++SF+G+AP NDPK A +V+I EP +AAPVF+ + L YMGV P+ Sbjct: 547 VASFVGFAPANDPKFAALVVIVEPSTGVYYGGQ--IAAPVFKSLGEDILRYMGVKPQEDP 604 Query: 120 ETADSTSKTVKAPAPAQRSAPDLTGKTMQAARQLLIDQGFDFEAVGEGTTVVSQYPEKGT 179 E A + P + P L G T+ A++ +I+ G F G G VV Q PE G Sbjct: 605 EPAKNEGPI--EVFPEEVRVPALEGLTLWEAQKKIIENGLIFNVEGHGPKVVKQSPEPGV 662 Query: 180 PLASGQRIYLLSQQG----DKPVIPDLKGQSLRDALEVLSLLKVGIAVEGEGYVSEQ 232 + G I L QG DK ++P+L G + + +L L +G G G EQ Sbjct: 663 IVPEGTEITLTLGQGKGDTDKVIVPELTGLTFDEVSGILKRLGLGFRYIGSGVAVEQ 719 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.312 0.131 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,398,414,447 Number of extensions: 56290632 Number of successful extensions: 135793 Number of sequences better than 1.0e-05: 1744 Number of HSP's gapped: 133730 Number of HSP's successfully gapped: 1744 Length of query: 269 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 136 Effective length of database: 1,807,701,208 Effective search space: 245847364288 Effective search space used: 245847364288 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 130 (54.7 bits)