BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0048.p (497 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG85.1 B3KG85_9BACI UDP-N-acetylmuramyl-tripeptide synthetase 716 0.0 C0ZGA9.1 C0ZGA9_BREBN UDP-N-acetylmuramoylalanyl-D-glutamate--2,... 528 e-148 C5D8L9.1 C5D8L9_9BACI UDP-N-acetylmuramyl-tripeptide synthetase 525 e-147 C3G3F3.1 C3G3F3_BACTU UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6... 513 e-143 C3E7G8.1 C3E7G8_BACTU UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-... 486 e-135 >B3KG85.1 B3KG85_9BACI UDP-N-acetylmuramyl-tripeptide synthetase Length = 494 Score = 716 bits (1848), Expect = 0.0, Method: Compositional matrix adjust. Identities = 364/493 (73%), Positives = 409/493 (82%) Query: 3 MKVNEFASCLAASRLYGDKETEITGLQADSRRVKPGDLFICLPGFTVDGHDFAPQAAANG 62 M + + +S LAA+ + G + EITG++ DSR+VKPGDLFICLPG TVDGH FA QAA G Sbjct: 1 MNLKQLSSVLAAAVIEGSGDVEITGIETDSRQVKPGDLFICLPGHTVDGHQFAAQAAGKG 60 Query: 63 ASALVCERKLDLDLPQIVVDDCRFAMAVLSNAFFGSPSSRMRMIXXXXXXXXXXXXYLIE 122 A+ALV ERKL++DLPQ++V D RFAMA L+NAF+ PSSRM+MI YLIE Sbjct: 61 AAALVVERKLEVDLPQVIVKDSRFAMAGLANAFYHQPSSRMKMIGVTGTNGKTTTTYLIE 120 Query: 123 RIMQDQNIKTGLIGTIQMRYDGQTFPASGTTQESLELQRTLSDMVQKGVQCCVMEVSSHA 182 IM D +KTGLIGTIQM+Y GQ+FP TT E+LELQR L+DM G +CCVMEVSSHA Sbjct: 121 TIMNDFGLKTGLIGTIQMKYGGQSFPMPRTTPEALELQRYLNDMANAGTECCVMEVSSHA 180 Query: 183 LQQGRVKGTDYRTAIFTNLTQDHLDYHHTMEEYRAVKGLFFSRLGNVISPWKEERKYAVL 242 L+QGRVKGTD+RTAIFTNLTQDHLDYHHTMEEYR KGLFFSRLGN + + RKYAVL Sbjct: 181 LEQGRVKGTDFRTAIFTNLTQDHLDYHHTMEEYRGAKGLFFSRLGNAFAKDESARKYAVL 240 Query: 243 NADDEASAYFAAQTAAEVITYGIDSPANVRASQISITSKGTFFHVDTFKGEADISLRMVG 302 NADD+ASAYFA QTAAEVITYG+ A+VRAS ISIT++GT FHVDTF+G ADIS+RMVG Sbjct: 241 NADDDASAYFAKQTAAEVITYGLGEDADVRASNISITAQGTSFHVDTFRGSADISIRMVG 300 Query: 303 KFNVYNALAAITAALLEDVPLEEIKASLESVAGVAGRVESVNEGQDFAVIVDYAHTPDGL 362 KFNVYNA+AAI AALLE VPLE+IK SLESV GV GRVE V+EGQ +AVIVDYAHTPDGL Sbjct: 301 KFNVYNAMAAIAAALLEGVPLEDIKNSLESVPGVDGRVEVVDEGQPYAVIVDYAHTPDGL 360 Query: 363 ENVLRAVCEFAAGRVLTVFGCGGDRDTTKRPLMGKIAAKYSDHVFVTSDNPRTEDPQLIL 422 ENVLR V EFA GRVLTVFGCGGDRD TKRP+MGKIAAKYSD VFVTSDNPRTEDP+LIL Sbjct: 361 ENVLRTVNEFAEGRVLTVFGCGGDRDRTKRPIMGKIAAKYSDVVFVTSDNPRTEDPELIL 420 Query: 423 KDIEAGLQEDGVAQDRYDMIVDRREAIRKAIEMASPSDVVLIAGKGHETYQLIGGVVHDF 482 KDIEAGL EDGVA + Y +IVDRR+AI KAIEMASP DVVLIAGKGHETYQLI G V DF Sbjct: 421 KDIEAGLVEDGVATESYKLIVDRRKAIEKAIEMASPGDVVLIAGKGHETYQLIAGEVLDF 480 Query: 483 DDRVVAKEVIRGR 495 DDR+VAK+ IRGR Sbjct: 481 DDRIVAKDAIRGR 493 >C0ZGA9.1 C0ZGA9_BREBN UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase EC=6.3.2.13 Length = 492 Score = 528 bits (1360), Expect = e-148, Method: Compositional matrix adjust. Identities = 271/475 (57%), Positives = 334/475 (70%), Gaps = 6/475 (1%) Query: 19 GDKETEITGLQADSRRVKPGDLFICLPGFTVDGHDFAPQAAANGASALVCERKLDLDLPQ 78 GD EITGL ADSR+VKPG LF+CL G+TVDGH FA QA NGA A++ E+ LD+ Sbjct: 17 GDDSMEITGLTADSRQVKPGYLFVCLTGYTVDGHTFAAQAVKNGAVAVLSEQDLDVPATI 76 Query: 79 IVVDDCRFAMAVLSNAFFGSPSSRMRMIXXXXXXXXXXXXYLIERIMQDQNIKTGLIGTI 138 + V D R AMA+L++ FGSP+ +++I +LI++I++DQ+ +TGLIGTI Sbjct: 77 VKVPDTRRAMAMLADRIFGSPTKEVKVIGVTGTNGKTTTTHLIDKILRDQSKQTGLIGTI 136 Query: 139 QMRYDGQTFPASGTTQESLELQRTLSDMVQKGVQCCVMEVSSHALQQGRVKGTDYRTAIF 198 MR T TT ++++LQR+ M ++EVSSHAL+ GRV+G + TA+F Sbjct: 137 HMRIGDVTEEVKNTTPDAIDLQRSFRRMCDVNTDYAIIEVSSHALELGRVRGCEIHTAVF 196 Query: 199 TNLTQDHLDYHHTMEEYRAVKGLFFSRLGNVISPWKEER-KYAVLNADDEASAYFAAQTA 257 TNLTQDHLDYH TME YR K L FS+LGN + +R K AVLNADDEAS +A T Sbjct: 197 TNLTQDHLDYHKTMENYRYAKSLLFSQLGN---GYDTDRLKTAVLNADDEASELYATVTP 253 Query: 258 AEVITYGIDSPANVRASQISITSKGTFFHVDTFKGEADISLRMVGKFNVYNALAAITAAL 317 A VITYGID PA+VRA QI ITSKGT F V++F G A ++LR++GKFNVYNALAAI L Sbjct: 254 ARVITYGIDQPADVRAKQIEITSKGTSFTVESFAGSARLNLRLMGKFNVYNALAAIAVTL 313 Query: 318 LEDVPLEEIKASLESVAGVAGRVESVNEGQDFAVIVDYAHTPDGLENVLRAVCEFAAGRV 377 E +PLE IKASLE VAGV GR E+V+ GQ FAV+VDY+HTPD LEN L V EFA G V Sbjct: 314 AEGIPLEAIKASLEEVAGVNGRFEAVDAGQPFAVLVDYSHTPDSLENALMTVKEFARGNV 373 Query: 378 LTVFGCGGDRDTTKRPLMGKIAAKYSDHVFVTSDNPRTEDPQLILKDIEAGLQEDGVAQD 437 + GCGGDRD TKRP+M +IA KY+D +TSDNPR+E+PQ IL D+ AGL E VA D Sbjct: 374 FCIVGCGGDRDRTKRPIMAQIATKYADLTVLTSDNPRSEEPQAILDDMLAGLSE--VAPD 431 Query: 438 RYDMIVDRREAIRKAIEMASPSDVVLIAGKGHETYQLIGGVVHDFDDRVVAKEVI 492 RY + DRREAI A+ +A P DV+LIAGKGHETYQ+I V FDDR VA+E I Sbjct: 432 RYTALTDRREAIAHAVSLAKPDDVILIAGKGHETYQIIKDQVLPFDDREVAREAI 486 >C5D8L9.1 C5D8L9_9BACI UDP-N-acetylmuramyl-tripeptide synthetase Length = 490 Score = 525 bits (1353), Expect = e-147, Method: Compositional matrix adjust. Identities = 267/495 (53%), Positives = 336/495 (67%), Gaps = 9/495 (1%) Query: 3 MKVNEFASCLAASRLYGDKETEITGLQADSRRVKPGDLFICLPGFTVDGHDFAPQAAANG 62 MK+ S L Y + IT ++ DSR+VK G LFIC+ GFTVDGHDFA QA NG Sbjct: 1 MKLQTLLSYLHDFTTYVGENPTITSIEMDSRQVKNGALFICIKGFTVDGHDFAKQAVENG 60 Query: 63 ASALVCERKLDLDLPQIVVDDCRFAMAVLSNAFFGSPSSRMRMIXXXXXXXXXXXXYLIE 122 A A++ ER LD+D+P IVV D R AMA+L++AF+G P+ R+ +I ++IE Sbjct: 61 AVAVIAERPLDVDVPVIVVRDSRRAMAMLADAFYGQPTHRLHLIGVTGTNGKTTTTHIIE 120 Query: 123 RIMQDQNIKTGLIGTIQMRYDGQTFPASGTTQESLELQRTLSDMVQKGVQCCVMEVSSHA 182 I + N KTGLIGT+ ++ T+P TT ESL LQRT MV +GV VMEVSSHA Sbjct: 121 HIARKANKKTGLIGTVHIKIGDHTYPTKNTTPESLLLQRTFKQMVDEGVDVAVMEVSSHA 180 Query: 183 LQQGRVKGTDYRTAIFTNLTQDHLDYHHTMEEYRAVKGLFFSRLGNVISPWKEER-KYAV 241 L GRV G DY A+FTNLTQDHLDYH TMEEYR KGL F++LGN + ER K+AV Sbjct: 181 LHMGRVHGCDYDVAVFTNLTQDHLDYHGTMEEYRNAKGLLFAQLGN---RYDHERPKFAV 237 Query: 242 LNADDEASAYFAAQTAAEVITYGIDSPANVRASQISITSKGTFFHVDTFKGEADISLRMV 301 LN DD AS Y+ TAA VITYGI++ +++ A +I I G F + T G + R++ Sbjct: 238 LNNDDPASKYYKHMTAASVITYGIETDSDIMAKKIDIVPSGMSFDLITPNGTVRVKTRLI 297 Query: 302 GKFNVYNALAAITAALLEDVPLEEIKASLESVAGVAGRVESVNEGQDFAVIVDYAHTPDG 361 G FNVYN LAA++A L+ +PL I ++E ++GVAGR E+V+EGQ+F VIVDYAHTPD Sbjct: 298 GLFNVYNLLAAVSACLVSGIPLPVIVEAIEEISGVAGRFETVDEGQNFTVIVDYAHTPDS 357 Query: 362 LENVLRAVCEFAAGRVLTVFGCGGDRDTTKRPLMGKIAAKYSDHVFVTSDNPRTEDPQLI 421 LENVL+ + +FA +V V GCGGDRD TKRPLM K A KY+D TSDNPR+EDP+ I Sbjct: 358 LENVLKTIRQFAQKKVYVVVGCGGDRDRTKRPLMAKTAVKYADVAIFTSDNPRSEDPEQI 417 Query: 422 LKDIEAGLQEDGVAQDRYDMIVDRREAIRKAIEMASPSDVVLIAGKGHETYQLIGGVVHD 481 L+D+EA GV +RY IVDR+EAI AIE A D++LIAGKGHETYQ+IG V D Sbjct: 418 LRDMEA-----GVPGERYVTIVDRKEAIYYAIEQAQEGDIILIAGKGHETYQIIGDRVID 472 Query: 482 FDDRVVAKEVIRGRS 496 FDDR+VA+E I+ R Sbjct: 473 FDDRLVAREAIKERK 487 >C3G3F3.1 C3G3F3_BACTU UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate ligase Length = 492 Score = 513 bits (1320), Expect = e-143, Method: Compositional matrix adjust. Identities = 255/494 (51%), Positives = 335/494 (67%), Gaps = 9/494 (1%) Query: 3 MKVNEFASCLAASRLYGDKETEITGLQADSRRVKPGDLFIC---LPGFTVDGHDFAPQAA 59 MK+ E AS L GD EITGL+ DSR++ GDLFIC + GF D H F A Sbjct: 1 MKLKELASLLLIKDTVGDMNVEITGLEMDSRKITSGDLFICVSGIDGFLEDRHQFVEDAV 60 Query: 60 ANGASALVCERKLDLDLPQIVVDDCRFAMAVLSNAFFGSPSSRMRMIXXXXXXXXXXXXY 119 NGA AL+ ER +++++P+IVV D R+AMAV+++ F+G PS++M++I Y Sbjct: 61 KNGAVALIVERDVNIEIPKIVVKDARYAMAVIASHFYGYPSNKMKLIGITGTNGKTTTSY 120 Query: 120 LIERIMQDQNIKTGLIGTIQMRYDGQTFPASGTTQESLELQRTLSDMVQKGVQCCVMEVS 179 L+E+I+ D TGL+G ++ D + +P TQE LQR L M + CVMEV+ Sbjct: 121 LLEKILSDYGYHTGLMGNNGVKVDSKWYPTDINTQEPPTLQRNLQMMKNQNADYCVMEVT 180 Query: 180 SHALQQGRVKGTDYRTAIFTNLTQDHLDYHHTMEEYRAVKGLFFSRLGNVISPWKEERKY 239 S L RVKG +++ A+FTNLT+DHLDYH T EY+ VKGL FSRLGN +S ++K Sbjct: 181 SQGLHMERVKGCNFKIAVFTNLTKDHLDYHGTFHEYKHVKGLLFSRLGNDLST--TDKKI 238 Query: 240 AVLNADDEASAYFAAQTAAEVITYGIDSPANVRASQISITSKGTFFHVDTFKGEADISLR 299 AVLNADD + YF T+AEVITYGI + A+V+A I+++ KG F V +F GE ++SL Sbjct: 239 AVLNADDPSVEYFKKITSAEVITYGIHNRADVQAKNITLSPKGIRFLVSSFNGEIEVSLN 298 Query: 300 MVGKFNVYNALAAITAALLEDVPLEEIKASLESVAGVAGRVESVNEGQDFAVIVDYAHTP 359 +VG+FNVYNALAAIT A++E +PL I SL ++ + GR+E ++E QDF VIVDYAHTP Sbjct: 299 LVGRFNVYNALAAITVAMVEGIPLTNICDSLSNLKSIEGRMEIIDENQDFLVIVDYAHTP 358 Query: 360 DGLENVLRAVCEFAAGRVLTVFGCGGDRDTTKRPLMGKIAAKYSDHVFVTSDNPRTEDPQ 419 D LENVL + EF+ G+V+TVFGCGGDRDT KR +MG IA YSD VF+TSDNPR+EDPQ Sbjct: 359 DALENVLSTISEFSTGKVITVFGCGGDRDTKKRSIMGGIAGSYSDFVFITSDNPRSEDPQ 418 Query: 420 LILKDIEAG-LQEDGVAQDRYDMIVDRREAIRKAIEMASPSDVVLIAGKGHETYQLIGGV 478 I+KDIE G LQ + + Y + VDR AI AI MAS +D+VLIAGKGHETYQ++ G Sbjct: 419 AIMKDIEKGFLQNNNL---NYKVEVDRELAITHAINMASSNDIVLIAGKGHETYQILKGS 475 Query: 479 VHDFDDRVVAKEVI 492 FDD+ A++ I Sbjct: 476 TIYFDDKEKARQAI 489 >C3E7G8.1 C3E7G8_BACTU UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase Length = 491 Score = 486 bits (1251), Expect = e-135, Method: Compositional matrix adjust. Identities = 248/493 (50%), Positives = 333/493 (67%), Gaps = 7/493 (1%) Query: 3 MKVNEFASCLAASRLYGDKETEITGLQADSRRVKPGDLFICLPGFTVDGHDFAPQAAANG 62 MK++ SCL + + EIT ++ADSR+VK G LF+C+ G+TVD HDFA QAAA G Sbjct: 1 MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQG 60 Query: 63 ASALVCERKLDLDLPQIVVDDCRFAMAVLSNAFFGSPSSRMRMIXXXXXXXXXXXXYLIE 122 A+A+V ER +D+D+P ++V + ++AVL++ F+G P+ ++ +I ++++ Sbjct: 61 AAAIVAERPIDVDVPVVLVKNTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMD 120 Query: 123 RIMQDQNIKTGLIGTIQMRYDGQTFPASGTTQESLELQRTLSDMVQKGVQCCVMEVSSHA 182 IM+ KTGLIGTI M+ +TF TT ++L LQ+T S MV++GV VMEVSSHA Sbjct: 121 EIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQTFSKMVEQGVDSTVMEVSSHA 180 Query: 183 LQQGRVKGTDYRTAIFTNLTQDHLDYHHTMEEYRAVKGLFFSRLGNVISPWKEERKYAVL 242 L GRV G DY A+FTNLTQDHLDYH TMEEY+ KGL F++LGN + +E KYAVL Sbjct: 181 LDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNRE--KYAVL 238 Query: 243 NADDEASAYFAAQTAAEVITYGIDSPANVRASQISITSKGTFFHVDTFKGEADISLRMVG 302 N+DD + + TAA V+TYGID+ +++ A I +TS GT F + T +++++++G Sbjct: 239 NSDDNVTEEYMRSTAATVVTYGIDTTSDIMAKNIVMTSGGTTFTLVTPNESVNVTMKLIG 298 Query: 303 KFNVYNALAAITAALLEDVPLEEIKASLESVAGVAGRVESVNEGQDFAVIVDYAHTPDGL 362 KFNVYN LAA A L+ V LE I A ++ +AGV GR E V+ GQ++ VIVDYAHTPD L Sbjct: 299 KFNVYNVLAATAAGLVSGVKLETIIAVIKDLAGVPGRFEVVDGGQNYTVIVDYAHTPDSL 358 Query: 363 ENVLRAVCEFAAGRVLTVFGCGGDRDTTKRPLMGKIAAKYSDHVFVTSDNPRTEDPQLIL 422 ENVL+ +FA G V + GCGGDRD TKRP+M +A KY+ H TSDNPR+EDP IL Sbjct: 359 ENVLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATKYATHAIYTSDNPRSEDPAAIL 418 Query: 423 KDIEAGLQEDGVAQDRYDMIVDRREAIRKAIEMASPSDVVLIAGKGHETYQLIGGVVHDF 482 D+ G + Y+MI+DR+EAI AI A D+++IAGKGHETYQ+IG VH F Sbjct: 419 DDM-----VHGASGKNYEMIIDRKEAIHHAIAKAKADDIIIIAGKGHETYQIIGKEVHHF 473 Query: 483 DDRVVAKEVIRGR 495 DDR VAKE I GR Sbjct: 474 DDREVAKEAITGR 486 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,716,917,088 Number of extensions: 107345300 Number of successful extensions: 286924 Number of sequences better than 1.0e-05: 3353 Number of HSP's gapped: 280685 Number of HSP's successfully gapped: 3562 Length of query: 497 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 357 Effective length of database: 1,741,754,103 Effective search space: 621806214771 Effective search space used: 621806214771 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)