BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0047.p (320 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG86.1 B3KG86_9BACI UDP-N-acetylmuramoyl-tripeptide--D-alanyl-... 338 4e-91 A9U869.1 A9U869_PHYPA Predicted protein (Fragment) 320 8e-86 Q62YL3.1 Q62YL3_BACLD UDP-N-acetylmuramoyl-tripeptide--D-alanyl-... 276 1e-72 Q65N64.1 Q65N64_BACLD UDP-N-acetylmuramoyl-tripeptide--D-alanyl-... 276 2e-72 A8FA56.1 A8FA56_BACP2 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-... 272 4e-71 >B3KG86.1 B3KG86_9BACI UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase EC=6.3.2.10 Length = 473 Score = 338 bits (867), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 170/298 (57%), Positives = 203/298 (68%) Query: 1 LITRTLRNIAAMCGGELSSAYDMDKSIAGVVTDSRKITPACLFVPLIGDHFDGHDYXXXX 60 +I ++L++IA MCGG LS D + I GV TDSR I P LFVP++G+ FDGH++ Sbjct: 1 MIHKSLKSIADMCGGTLSRNQDDNIIIQGVGTDSRTIQPGNLFVPIVGEKFDGHEFVESV 60 Query: 61 XXXXXXXTLWQRDKGAAPENGSVILVDDTLTALQKLASAYLSQVAPQVVAVTGSNGKTTT 120 WQRD P + +I V+DTL ALQ LA+AYLS+ +VV +TGSNGKTT Sbjct: 61 LAGGAGAAFWQRDHQPVPADLPLIFVEDTLKALQSLAAAYLSESRAKVVGITGSNGKTTV 120 Query: 121 KDMITALLEGQYKVHKTQGNFNNHIGLPLTILSMSGDVQIAVLEMGMSSRGEIXXXXXXX 180 KDM++ALLE Y VHKTQGN+NNHIGLPLT+L+M D I VLEMGMS R EI Sbjct: 121 KDMVSALLETTYNVHKTQGNYNNHIGLPLTVLAMKEDTDIIVLEMGMSGRHEIELLSNIA 180 Query: 181 PPDVAVITNVGESHLLQLGSRKEIARAKLEIAEGLKPGGLLIYNGDEPLLSEVMAESAFR 240 PD+ VITNVGE+HLLQLGSR+EIARAKLEI G+KPGGLLIY+GDEPL+ +V+AE A Sbjct: 181 RPDLTVITNVGEAHLLQLGSREEIARAKLEIISGMKPGGLLIYHGDEPLIRQVLAEEATV 240 Query: 241 APEGMRTFRFGLSPDNTDYPXXXXXXXXXXXXXSHLHAEHAFTLPLPGRHNVVNALAA 298 PEG+RTF FG N DYP S HA F+LPL GRHNVVNALAA Sbjct: 241 KPEGLRTFTFGNQDTNDDYPTGLMFHARGIIFTSSRHAGEGFSLPLLGRHNVVNALAA 298 >A9U869.1 A9U869_PHYPA Predicted protein (Fragment) Length = 345 Score = 320 bits (821), Expect = 8e-86, Method: Compositional matrix adjust. Identities = 164/272 (60%), Positives = 187/272 (68%), Gaps = 1/272 (0%) Query: 27 IAGVVTDSRKITPACLFVPLIGDHFDGHDYXXXXXXXXXXXTLWQRDKGAAPENGSVILV 86 I GV TDSR+I LFVPL+G+ FDGH Y TLWQRD+G AP G+VI+V Sbjct: 8 IQGVFTDSRQIKQGSLFVPLVGERFDGHAYAESVLKDGAGATLWQRDRGEAPA-GAVIVV 66 Query: 87 DDTLTALQKLASAYLSQVAPQVVAVTGSNGKTTTKDMITALLEGQYKVHKTQGNFNNHIG 146 DDTL ALQ LA AYL++V +VV +TGSNGKTTTKDM+ LL YKVHKT GNFNNHIG Sbjct: 67 DDTLAALQTLAEAYLAEVGCRVVGITGSNGKTTTKDMVYTLLSTTYKVHKTGGNFNNHIG 126 Query: 147 LPLTILSMSGDVQIAVLEMGMSSRGEIXXXXXXXPPDVAVITNVGESHLLQLGSRKEIAR 206 LPLTIL M D +IAVLEMGMS R EI PDVAVITN+GE+HLLQLGSR EIAR Sbjct: 127 LPLTILEMPADTEIAVLEMGMSGRHEIERLSQIARPDVAVITNIGEAHLLQLGSRHEIAR 186 Query: 207 AKLEIAEGLKPGGLLIYNGDEPLLSEVMAESAFRAPEGMRTFRFGLSPDNTDYPXXXXXX 266 AK+EI G+KPGGLLIY+GDEPL+ V+ E+ PEG+ TF FGL N DYP Sbjct: 187 AKVEIISGMKPGGLLIYHGDEPLIPMVLGEAETVKPEGLGTFTFGLDHSNDDYPTGMMFH 246 Query: 267 XXXXXXXSHLHAEHAFTLPLPGRHNVVNALAA 298 S LH E LPL G+HNV+N LAA Sbjct: 247 SKGIIFTSLLHPEEGLDLPLLGQHNVINCLAA 278 >Q62YL3.1 Q62YL3_BACLD UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase EC=6.3.2.10 Length = 456 Score = 276 bits (707), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 151/298 (50%), Positives = 188/298 (63%), Gaps = 7/298 (2%) Query: 1 LITRTLRNIAAMCGGELSSAYDMDKSIAGVVTDSRKITPACLFVPLIGDHFDGHDYXXXX 60 +ITRT++ IA M G L++ + I GV TDSRK+ LF+PL G+ F+GH + Sbjct: 1 MITRTVKEIAEMSEGVLTNEAFEGRLIKGVSTDSRKLAKGQLFIPLAGERFNGHVFAEQA 60 Query: 61 XXXXXXXTLWQRDKGAAPENGSVILVDDTLTALQKLASAYLSQVAPQVVAVTGSNGKTTT 120 LW R + PE VILVDDTL ALQKLA AY +Q+ +VV VTGSNGKTTT Sbjct: 61 FQEGVAAVLWNRSEPNPPEGKPVILVDDTLEALQKLAKAYRNQLDVKVVGVTGSNGKTTT 120 Query: 121 KDMITALLEGQYKVHKTQGNFNNHIGLPLTILSMSGDVQIAVLEMGMSSRGEIXXXXXXX 180 KDMI A+L +YKVHKT+GN+NNHIGLPLTIL+M D ++A+LEMGMS++GEI Sbjct: 121 KDMIHAVLGTEYKVHKTEGNYNNHIGLPLTILAMPEDTEVAILEMGMSAKGEIDFLTRLA 180 Query: 181 PPDVAVITNVGESHLLQLGSRKEIARAKLEIAEGLKPGGLLIYNGDEPLLSEVMAESAFR 240 PD+AVITN+GESHLL+LGSR+ IA AKLEI GL G LIY GDEPLL+E + + + Sbjct: 181 RPDIAVITNIGESHLLELGSREGIAEAKLEIVNGLSKEGTLIYVGDEPLLTERLNQPPY- 239 Query: 241 APEGMRTFRFGLSPDNTDYPXXXXXXXXXXXXXSHLHAEHAFTLPLPGRHNVVNALAA 298 T FG P DY E+ F +P+ G+HNV NALAA Sbjct: 240 -----DTKTFGEDP-AFDYQLEDMTQTEEGTAFRVRGIENGFFIPVLGKHNVKNALAA 291 >Q65N64.1 Q65N64_BACLD UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase EC=6.3.2.10 Length = 491 Score = 276 bits (705), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 151/298 (50%), Positives = 188/298 (63%), Gaps = 7/298 (2%) Query: 1 LITRTLRNIAAMCGGELSSAYDMDKSIAGVVTDSRKITPACLFVPLIGDHFDGHDYXXXX 60 +ITRT++ IA M G L++ + I GV TDSRK+ LF+PL G+ F+GH + Sbjct: 36 MITRTVKEIAEMSEGVLTNEAFEGRLIKGVSTDSRKLAKGQLFIPLAGERFNGHVFAEQA 95 Query: 61 XXXXXXXTLWQRDKGAAPENGSVILVDDTLTALQKLASAYLSQVAPQVVAVTGSNGKTTT 120 LW R + PE VILVDDTL ALQKLA AY +Q+ +VV VTGSNGKTTT Sbjct: 96 FQEGVAAVLWNRSEPNPPEGKPVILVDDTLEALQKLAKAYRNQLDVKVVGVTGSNGKTTT 155 Query: 121 KDMITALLEGQYKVHKTQGNFNNHIGLPLTILSMSGDVQIAVLEMGMSSRGEIXXXXXXX 180 KDMI A+L +YKVHKT+GN+NNHIGLPLTIL+M D ++A+LEMGMS++GEI Sbjct: 156 KDMIHAVLGTEYKVHKTEGNYNNHIGLPLTILAMPEDTEVAILEMGMSAKGEIDFLTRLA 215 Query: 181 PPDVAVITNVGESHLLQLGSRKEIARAKLEIAEGLKPGGLLIYNGDEPLLSEVMAESAFR 240 PD+AVITN+GESHLL+LGSR+ IA AKLEI GL G LIY GDEPLL+E + + + Sbjct: 216 RPDIAVITNIGESHLLELGSREGIAEAKLEIVNGLSKEGTLIYVGDEPLLTERLNQPPY- 274 Query: 241 APEGMRTFRFGLSPDNTDYPXXXXXXXXXXXXXSHLHAEHAFTLPLPGRHNVVNALAA 298 T FG P DY E+ F +P+ G+HNV NALAA Sbjct: 275 -----DTKTFGEDP-AFDYQLEDMTQTEEGTAFRVRGIENGFFIPVLGKHNVKNALAA 326 >A8FA56.1 A8FA56_BACP2 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase EC=6.3.2.10 Length = 454 Score = 272 bits (695), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 144/298 (48%), Positives = 184/298 (61%), Gaps = 7/298 (2%) Query: 1 LITRTLRNIAAMCGGELSSAYDMDKSIAGVVTDSRKITPACLFVPLIGDHFDGHDYXXXX 60 +I RT++ IA M GG LS+ ++ I GV TD+RK++ LF+PLIG+HF+GH + Sbjct: 1 MIKRTVKQIAQMAGGTLSNQAFGEEQIHGVTTDTRKVSKGALFIPLIGEHFNGHTFASKA 60 Query: 61 XXXXXXXTLWQRDKGAAPENGSVILVDDTLTALQKLASAYLSQVAPQVVAVTGSNGKTTT 120 LW + + PE VILVDDTL ALQ+LA +YL + P+VV +TGSNGKTTT Sbjct: 61 VELGAAAVLWNQKETNPPEGVPVILVDDTLAALQQLAKSYLKEENPRVVGITGSNGKTTT 120 Query: 121 KDMITALLEGQYKVHKTQGNFNNHIGLPLTILSMSGDVQIAVLEMGMSSRGEIXXXXXXX 180 KDMI ++L+ Y+VHKT GNFNNHIGLPLTILSM +IAVLEMGMS++GEI Sbjct: 121 KDMIHSVLQTTYQVHKTDGNFNNHIGLPLTILSMPKGTEIAVLEMGMSAKGEIEFLSNLA 180 Query: 181 PPDVAVITNVGESHLLQLGSRKEIARAKLEIAEGLKPGGLLIYNGDEPLLSEVMAESAFR 240 PD AVITN+GESH+ LGSR+ IA AK EI +GLK G Y GDEPL+ E A + Sbjct: 181 EPDAAVITNIGESHMQDLGSREAIADAKCEITKGLKEDGTFYYLGDEPLIR----ERATQ 236 Query: 241 APEGMRTFRFGLSPDNTDYPXXXXXXXXXXXXXSHLHAEHAFTLPLPGRHNVVNALAA 298 + ++TF D+ D F +P+ G+HNV NALAA Sbjct: 237 LSQQVKTFG---EADDCDIKVTHINQLAEGTEFQVEGYAQGFLIPVLGKHNVKNALAA 291 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,630,762,148 Number of extensions: 60333268 Number of successful extensions: 136142 Number of sequences better than 1.0e-05: 1758 Number of HSP's gapped: 134809 Number of HSP's successfully gapped: 1758 Length of query: 320 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 185 Effective length of database: 1,788,859,178 Effective search space: 330938947930 Effective search space used: 330938947930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)