BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0046.p (147 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG86.1 B3KG86_9BACI UDP-N-acetylmuramoyl-tripeptide--D-alanyl-... 165 1e-39 A6CS96.1 A6CS96_9BACI UDP-N-acetylmuramoyl-tripeptide--D-alanyl-... 158 1e-37 A8FA56.1 A8FA56_BACP2 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-... 148 1e-34 B4AIL4.1 B4AIL4_BACPU UDP-N-acetylmuramoyl-tripeptide--D-alanyl-... 148 1e-34 Q73EU2.1 Q73EU2_BACC1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-... 142 9e-33 >B3KG86.1 B3KG86_9BACI UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase EC=6.3.2.10 Length = 473 Score = 165 bits (418), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 82/138 (59%), Positives = 103/138 (74%) Query: 1 MRIEMMQAASGLTMLNDAYNASPTSMRAAIDVLQAMKCSGKRIAVLGDMLELGPDEVLFH 60 MRIE ++ A G+T+LNDAYN+SPT+++AA+DVL MK +RIAVLGDMLELG E FH Sbjct: 324 MRIEAVETAFGVTVLNDAYNSSPTAVKAAVDVLGDMKGYRRRIAVLGDMLELGQREESFH 383 Query: 61 EEIGRYLDPAQTDLLFTFGPLSAHTARKAEERFGPERVFAFTDKKELTAALTSQIDPRDV 120 EIG YL P DL+FT+GPLS HTA KA+ RF V +FTDK++L AL Q+ +D+ Sbjct: 384 AEIGEYLSPDNADLVFTYGPLSLHTANKAKARFPEGAVLSFTDKEKLIEALLEQVTEKDI 443 Query: 121 VLFKASRGMRLEEVLHSL 138 VL KASRGMRLE V+ +L Sbjct: 444 VLVKASRGMRLEHVVDAL 461 >A6CS96.1 A6CS96_9BACI UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase EC=6.3.2.10 Length = 458 Score = 158 bits (399), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 75/140 (53%), Positives = 101/140 (72%) Query: 1 MRIEMMQAASGLTMLNDAYNASPTSMRAAIDVLQAMKCSGKRIAVLGDMLELGPDEVLFH 60 MR+EMM+ G +LNDAYNASPTSMRAAI +L ++ GK+I V GDMLELGP+E +H Sbjct: 319 MRMEMMEGKRGEKILNDAYNASPTSMRAAIGLLSGLQGYGKKILVAGDMLELGPNEENYH 378 Query: 61 EEIGRYLDPAQTDLLFTFGPLSAHTARKAEERFGPERVFAFTDKKELTAALTSQIDPRDV 120 E+G+ + + DL+FTFG L+ TAR A E G + VF+F DK+ELT L S + ++ Sbjct: 379 YEVGQEVQKGKIDLVFTFGTLARQTARGAAENIGEKNVFSFDDKQELTEKLESILHGDEI 438 Query: 121 VLFKASRGMRLEEVLHSLND 140 +LFKASRGM+LEE++ SL + Sbjct: 439 ILFKASRGMKLEEIIESLTE 458 >A8FA56.1 A8FA56_BACP2 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase EC=6.3.2.10 Length = 454 Score = 148 bits (374), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 73/138 (52%), Positives = 95/138 (68%) Query: 1 MRIEMMQAASGLTMLNDAYNASPTSMRAAIDVLQAMKCSGKRIAVLGDMLELGPDEVLFH 60 MR+E+ + G+T++NDAYNASPTSM+AAID++ M RI VLGDMLE+G +E +H Sbjct: 317 MRLELFKTEKGITVINDAYNASPTSMKAAIDLVGDMDGFANRILVLGDMLEMGSEEETYH 376 Query: 61 EEIGRYLDPAQTDLLFTFGPLSAHTARKAEERFGPERVFAFTDKKELTAALTSQIDPRDV 120 E+GRY+ P D + T+G L A A A++ FG RVF+F DK+EL L P DV Sbjct: 377 YELGRYIKPEFIDHVMTYGRLGAFIAEGAKKSFGDTRVFSFMDKEELKKKLADVAGPDDV 436 Query: 121 VLFKASRGMRLEEVLHSL 138 VL KASRGMRLEEV+ +L Sbjct: 437 VLVKASRGMRLEEVITAL 454 >B4AIL4.1 B4AIL4_BACPU UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase EC=6.3.2.10 Length = 454 Score = 148 bits (374), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 73/138 (52%), Positives = 95/138 (68%) Query: 1 MRIEMMQAASGLTMLNDAYNASPTSMRAAIDVLQAMKCSGKRIAVLGDMLELGPDEVLFH 60 MR+E+ + G+T++NDAYNASPTSM+AAID++ M RI VLGDMLE+G +E +H Sbjct: 317 MRLELFKTEKGITVINDAYNASPTSMKAAIDLVGDMDGFANRILVLGDMLEMGSEEETYH 376 Query: 61 EEIGRYLDPAQTDLLFTFGPLSAHTARKAEERFGPERVFAFTDKKELTAALTSQIDPRDV 120 E+GRY+ P D + T+G L A A A++ FG RVF+F DK+EL L P DV Sbjct: 377 YELGRYIKPEFIDHVMTYGRLGAFIAEGAKKSFGDARVFSFMDKEELKKKLADVAGPDDV 436 Query: 121 VLFKASRGMRLEEVLHSL 138 VL KASRGMRLEEV+ +L Sbjct: 437 VLVKASRGMRLEEVITAL 454 >Q73EU2.1 Q73EU2_BACC1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase EC=6.3.2.10 Length = 458 Score = 142 bits (358), Expect = 9e-33, Method: Composition-based stats. Identities = 71/138 (51%), Positives = 97/138 (70%) Query: 1 MRIEMMQAASGLTMLNDAYNASPTSMRAAIDVLQAMKCSGKRIAVLGDMLELGPDEVLFH 60 MR+E+++ SGLT++NDAYNASPT+M AA ++ + K+I VLGDMLELG EV FH Sbjct: 320 MRMEIVKTNSGLTIINDAYNASPTAMEAAFHLMNGLDGFSKKIVVLGDMLELGDQEVQFH 379 Query: 61 EEIGRYLDPAQTDLLFTFGPLSAHTARKAEERFGPERVFAFTDKKELTAALTSQIDPRDV 120 E+G+ +DPA+ +FT+G L A A A+ F ERV A+ +K+EL L + +D +DV Sbjct: 380 YEVGKLIDPAKISYVFTYGKLGAQIAEGAKINFPNERVKAYDNKEELVKELQAVVDAKDV 439 Query: 121 VLFKASRGMRLEEVLHSL 138 VL KASRGM+LEEV+ L Sbjct: 440 VLIKASRGMKLEEVITML 457 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.132 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 794,750,527 Number of extensions: 29738041 Number of successful extensions: 75089 Number of sequences better than 1.0e-05: 1320 Number of HSP's gapped: 73811 Number of HSP's successfully gapped: 1320 Length of query: 147 Length of database: 3,060,696,203 Length adjustment: 110 Effective length of query: 37 Effective length of database: 2,024,384,553 Effective search space: 74902228461 Effective search space used: 74902228461 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)