BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0045.p (307 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG87.1 B3KG87_9BACI Phospho-N-acetylmuramoyl-pentapeptide-tran... 415 e-114 B1DMA2.1 B1DMA2_9BACL Phospho-N-acetylmuramoyl-pentapeptide-tran... 358 5e-97 Q9K9S6.2 MRAY_BACHD Phospho-N-acetylmuramoyl-pentapeptide-transf... 298 3e-79 A7Z4E2.1 MRAY_BACA2 Phospho-N-acetylmuramoyl-pentapeptide-transf... 296 2e-78 B4BJE3.1 B4BJE3_9BACI Phospho-N-acetylmuramoyl-pentapeptide-tran... 293 1e-77 >B3KG87.1 B3KG87_9BACI Phospho-N-acetylmuramoyl-pentapeptide-transferase EC=2.7.8.13 Length = 321 Score = 415 bits (1067), Expect = e-114, Method: Compositional matrix adjust. Identities = 203/297 (68%), Positives = 235/297 (79%) Query: 1 MDYQLLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIM 60 MD+QLLLLTI V+F+LAVI+APL IPLLRRMKFGQQVR+DGPQ+HLKKAGTPTMGG++I+ Sbjct: 1 MDFQLLLLTIGVAFVLAVISAPLLIPLLRRMKFGQQVRNDGPQSHLKKAGTPTMGGVVIL 60 Query: 61 VAFTLSFLKFSVVNSDFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQLLVG 120 +AFTL+++KFS V++DFYVL+VA LG+GLIGFLDDYIKI F+RSLGLT RQKL GQLL Sbjct: 61 LAFTLTYIKFSPVDTDFYVLIVATLGFGLIGFLDDYIKIVFRRSLGLTARQKLFGQLLFS 120 Query: 121 AVMCGLLINAGHNTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVDGLLSGX 180 +MC LLI+ GH+T IS+PG SFDWG WFYYPFI++MM+A++NAVNFTDG+DGLLSG Sbjct: 121 GIMCWLLISNGHSTAISVPGMDWSFDWGPWFYYPFIVVMMLAISNAVNFTDGLDGLLSGV 180 Query: 181 XXXXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXXXXXXXX 240 TS LGFLVFNAHPAKVFMGD GS Sbjct: 181 SAIAFGAYAIVAIQSTSFAAAFCAAAMIGAVLGFLVFNAHPAKVFMGDTGSLGIGGAIGA 240 Query: 241 XXXVTKSELLFVVIGGVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGGWSEW 297 VTKSELLF++IGGVFVIEMLSVVLQV SFKTRGKRIF+MSPIHHH+EL GWSEW Sbjct: 241 IAIVTKSELLFLIIGGVFVIEMLSVVLQVISFKTRGKRIFKMSPIHHHYELSGWSEW 297 >B1DMA2.1 B1DMA2_9BACL Phospho-N-acetylmuramoyl-pentapeptide-transferase EC=2.7.8.13 Length = 318 Score = 358 bits (918), Expect = 5e-97, Method: Compositional matrix adjust. Identities = 176/297 (59%), Positives = 213/297 (71%), Gaps = 1/297 (0%) Query: 1 MDYQLLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIM 60 MD ++L ++ SF++AV+ PLFIPLLRRMKFGQQ+R DGPQ+HLKK+GTPTMGGIIIM Sbjct: 1 MDTMVILFSLGASFLIAVLLGPLFIPLLRRMKFGQQIRTDGPQSHLKKSGTPTMGGIIIM 60 Query: 61 VAFTLSFLKFSVVNSDFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQLLVG 120 +A ++FLKFS DF+VL A+LG+GL+GFLDDYIKI FKRSLGLT RQKL GQLL Sbjct: 61 LALLIAFLKFSDKTPDFWVLFTASLGFGLVGFLDDYIKIVFKRSLGLTARQKLFGQLLFS 120 Query: 121 AVMCGLLINAGHNTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVDGLLSGX 180 ++C L N H+T I++PGTS FD GWFYYPF++I++ +NAVNFTDG+DGLLSG Sbjct: 121 IIVCAELYNMNHSTMITVPGTSWGFDL-GWFYYPFVVIILFGSSNAVNFTDGLDGLLSGT 179 Query: 181 XXXXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXXXXXXXX 240 + LGFL+FNAHPAKVFMGD GS Sbjct: 180 SAIAFGAFTILALQVSEHESAIFSAAMVGAVLGFLIFNAHPAKVFMGDTGSLGIGGGIAA 239 Query: 241 XXXVTKSELLFVVIGGVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGGWSEW 297 +TK EL+ V++GGVFV+EMLSV+LQV SFK RGKRIF+MSPIHHHFEL GWSEW Sbjct: 240 VAILTKMELMLVIVGGVFVLEMLSVILQVGSFKLRGKRIFKMSPIHHHFELSGWSEW 296 >Q9K9S6.2 MRAY_BACHD Phospho-N-acetylmuramoyl-pentapeptide-transferase EC=2.7.8.13 Length = 325 Score = 298 bits (764), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 146/301 (48%), Positives = 207/301 (68%), Gaps = 5/301 (1%) Query: 1 MDYQLLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIM 60 M ++LLLT+ +SF++ VI +P+FIP LRR+KFGQ +R++GP++H KK+GTPTMGG++I+ Sbjct: 1 MLERVLLLTLILSFVITVILSPIFIPFLRRLKFGQSIREEGPKSHQKKSGTPTMGGLMIL 60 Query: 61 VAFTLSFL----KFSVVNSDFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQ 116 ++ +S L + S+ + D +LL+ +G+G++GF+DD+IK+ KR+LGLT +QKL+GQ Sbjct: 61 LSILVSSLFVSFQLSIFSMDVLLLLLVTIGFGVLGFIDDFIKVVMKRNLGLTSKQKLIGQ 120 Query: 117 LLVGAVMCGLLINAGHNTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVDGL 176 L+V + L N G +T ++IP TS+S D+G WFY P +I+M++ +NAVN TDG+DGL Sbjct: 121 LVVAVLFYLGLRNMGLSTEVTIPATSLSIDFG-WFYLPLVIVMLVGASNAVNLTDGLDGL 179 Query: 177 LSGXXXXXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXXXX 236 ++G LGFLVFN+HPAKVFMGD GS Sbjct: 180 VAGTGAIAFGAFAIIAWATNYFEVAIFSAAVVGAVLGFLVFNSHPAKVFMGDTGSLALGG 239 Query: 237 XXXXXXXVTKSELLFVVIGGVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGGWSE 296 + K E+L ++IGGVFVIE LSV++QV SFKTRGKRIF+MSP+HHH+EL GWSE Sbjct: 240 AIAAIAIMAKQEILLIIIGGVFVIETLSVIIQVISFKTRGKRIFKMSPLHHHYELSGWSE 299 Query: 297 W 297 W Sbjct: 300 W 300 >A7Z4E2.1 MRAY_BACA2 Phospho-N-acetylmuramoyl-pentapeptide-transferase EC=2.7.8.13 Length = 324 Score = 296 bits (757), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 147/301 (48%), Positives = 205/301 (68%), Gaps = 5/301 (1%) Query: 1 MDYQLLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIM 60 M Q++L TI + F+++V+ +P+ IP LRR+KFGQ +R++GP++H+KK+GTPTMGG++I+ Sbjct: 1 MLEQVILFTIIMGFLISVLISPILIPFLRRLKFGQSIREEGPKSHMKKSGTPTMGGVMII 60 Query: 61 VAFTLSFL----KFSVVNSDFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQ 116 V+ ++ + KFS ++++ ++LL +GYGL+GFLDDYIK+ KR+LGLT +QKL+GQ Sbjct: 61 VSIAITAIVMTQKFSHLSAEMFLLLFVTIGYGLLGFLDDYIKVVMKRNLGLTSKQKLIGQ 120 Query: 117 LLVGAVMCGLLINAGHNTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVDGL 176 +++ V G+ +TGI +PGT +S D G W Y+ ++ M++ +NAVN TDG+DGL Sbjct: 121 IIIAIVFYGVYHYCHFSTGIRVPGTHLSIDLG-WGYFILVLFMLVGGSNAVNLTDGLDGL 179 Query: 177 LSGXXXXXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXXXX 236 LSG + LGFLVFNAHPAKVFMGD GS Sbjct: 180 LSGTAAIAFGAFAILAWNQSQYDVAIFSVAVVGAVLGFLVFNAHPAKVFMGDTGSLALGG 239 Query: 237 XXXXXXXVTKSELLFVVIGGVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGGWSE 296 +TK E+L V+IGGVFVIE LSV+LQV SFKT GKRIF+MSP+HHH+EL GWSE Sbjct: 240 AIVTVAILTKLEILLVIIGGVFVIETLSVILQVISFKTTGKRIFKMSPLHHHYELVGWSE 299 Query: 297 W 297 W Sbjct: 300 W 300 >B4BJE3.1 B4BJE3_9BACI Phospho-N-acetylmuramoyl-pentapeptide-transferase EC=2.7.8.13 Length = 324 Score = 293 bits (750), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 150/301 (49%), Positives = 201/301 (66%), Gaps = 5/301 (1%) Query: 1 MDYQLLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIM 60 M Q++++ +AVSF++ VI +PLFIP LRR+KFGQ +R++GP++H KK+GTPTMGGI+I+ Sbjct: 1 MPEQIIVIAMAVSFLITVILSPLFIPFLRRLKFGQSIREEGPKSHQKKSGTPTMGGIMIL 60 Query: 61 VAFTLSFL----KFSVVNSDFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQ 116 ++ + + KFSV+++ Y+LL +GYG++GFLDD IK+ KR+LGLT RQK +GQ Sbjct: 61 LSIIATTVWVTAKFSVLSAGTYLLLFVTIGYGVLGFLDDMIKVVMKRNLGLTSRQKFIGQ 120 Query: 117 LLVGAVMCGLLINAGHNTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVDGL 176 L++ + + +G +T + IPGT SFD G W Y ++ M++ +NAVN TDG+DGL Sbjct: 121 LIIAVIFFFVYRQSGFSTALHIPGTDWSFDLG-WAYGVLLLFMLVGGSNAVNLTDGLDGL 179 Query: 177 LSGXXXXXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXXXX 236 L+G LGFLVFNAHPAKVFMGD GS Sbjct: 180 LAGTAAIAFGAYAVLAWNQGQYDVAVFCVAVVGAVLGFLVFNAHPAKVFMGDTGSLALGG 239 Query: 237 XXXXXXXVTKSELLFVVIGGVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGGWSE 296 +TK ELL VVIGGVFVIE LSV++QVASFK GKR+FRMSP+HHH+EL GWSE Sbjct: 240 AIAAVAVLTKLELLLVVIGGVFVIETLSVIIQVASFKMTGKRVFRMSPLHHHYELVGWSE 299 Query: 297 W 297 W Sbjct: 300 W 300 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.146 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,492,131,907 Number of extensions: 54091394 Number of successful extensions: 175863 Number of sequences better than 1.0e-05: 1898 Number of HSP's gapped: 171807 Number of HSP's successfully gapped: 2917 Length of query: 307 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 172 Effective length of database: 1,788,859,178 Effective search space: 307683778616 Effective search space used: 307683778616 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)