BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0044.p (472 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG88.1 B3KG88_9BACI UDP-N-acetylmuramoylalanine--D-glutamate l... 655 0.0 B1DMA3.1 B1DMA3_9BACL UDP-N-acetylmuramoylalanine--D-glutamate l... 558 e-157 C0ZGA7.1 C0ZGA7_BREBN UDP-N-acetylmuramoylalanine--D-glutamate l... 421 e-115 C5D8M2.1 C5D8M2_9BACI UDP-N-acetylmuramoylalanine/D-glutamate li... 412 e-113 A4IM07.1 A4IM07_GEOTN UDP-N-acetylmuramoylalanine--D-glutamate l... 392 e-107 >B3KG88.1 B3KG88_9BACI UDP-N-acetylmuramoylalanine--D-glutamate ligase EC=6.3.2.9 Length = 477 Score = 655 bits (1691), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/477 (68%), Positives = 377/477 (79%), Gaps = 5/477 (1%) Query: 1 MEHPDQYRGKEVVVLGLAKSGVQVAKVLHERGADVTVNDKKERDQSPEASELESLGISVI 60 M+ D YRGK +VVLGLAKSGVQVAKVLH+ GA V VND+KER+Q PEASELE+LGISVI Sbjct: 1 MKQLDDYRGKHIVVLGLAKSGVQVAKVLHQAGAYVIVNDRKEREQCPEASELEALGISVI 60 Query: 61 CGGHPEGLIHPGVSLVVKNPGIPYSVPPVQKALELGIEVVTEVEVAYHLCAAPMIGITGS 120 CGGHP+ LIHPGV+L+VKNPGIPYSVPPVQKA+EL I +VTEVE+AY LC APMIGITGS Sbjct: 61 CGGHPDELIHPGVALLVKNPGIPYSVPPVQKAIELEIPIVTEVEIAYRLCEAPMIGITGS 120 Query: 121 NGKXXXXXWVGRMLDAAGMHPIVAGNIGTPLCQAAQEAHADNWMVVELSSFQLKGTKAFR 180 NGK WVG++L+ AG+ PIVAGNIGTPL +AA A +WMVVELSSFQLKGT+AFR Sbjct: 121 NGKTTTTTWVGKLLENAGLSPIVAGNIGTPLSEAAATATEHDWMVVELSSFQLKGTEAFR 180 Query: 181 PKVGCLLNVAETHLDYHGGMEDYIASKAKLFANQGPSDTAVLNWDDPVCRELVPYIKAGI 240 P++ CLLNV ETHLDYHGGM+DY+ASKAKLFANQ DTAVLNWDDPVCR LVPYIKA I Sbjct: 181 PRISCLLNVTETHLDYHGGMDDYVASKAKLFANQTSEDTAVLNWDDPVCRNLVPYIKAHI 240 Query: 241 LPFSMTEELVQGVFVRPAFVP---DTEDDLK-RMIVYRDYSESETEIAAVDSIGLPGRFN 296 +PFSM+E L +GV + P + P D+++D + R I+Y D EI V++IGLPGRFN Sbjct: 241 VPFSMSESLREGVHITPPYAPGAQDSDEDCQGRRIIYTDGEGFTHEIIRVENIGLPGRFN 300 Query: 297 VEXXXXXXXXXXXXXXXXXXXXEVLVNFRGVEHRLEYVAEKAGAAYYNNSKATNSKATSM 356 V E L +FRGVEHRLE+VAE G +YYNNSKATNSKAT M Sbjct: 301 VGNAMAACAIAIAAGVAPDKLEEALSSFRGVEHRLEFVAELQGVSYYNNSKATNSKATHM 360 Query: 357 ALASFRKPVVLIAGGLDRGSDYMELLPVLGG-VKALVALGQTRDKLAAVAGMAGVKRIIS 415 AL SF++PVVLIAGGLDRGSDY EL+P + G VKA+V LG+TR+K+A A AG+K II Sbjct: 361 ALTSFKEPVVLIAGGLDRGSDYEELIPSMQGRVKAVVLLGETREKIANAAAKAGLKDIIL 420 Query: 416 VDNGESAAAVLQEAVREASALAEAGDIVLLSPACASWDMFTSYEERGRIFKEAVHNL 472 VDNGE AA+ L AVR+A+ LAE+GDIVLLSPACASWDMF SYE RGRIFKEAVHNL Sbjct: 421 VDNGEDAASTLTSAVRKAAGLAESGDIVLLSPACASWDMFPSYETRGRIFKEAVHNL 477 >B1DMA3.1 B1DMA3_9BACL UDP-N-acetylmuramoylalanine--D-glutamate ligase EC=6.3.2.9 Length = 473 Score = 558 bits (1437), Expect = e-157, Method: Compositional matrix adjust. Identities = 277/473 (58%), Positives = 334/473 (70%), Gaps = 1/473 (0%) Query: 1 MEHPDQYRGKEVVVLGLAKSGVQVAKVLHERGADVTVNDKKERDQSPEASELESLGISVI 60 M HP YR + VVVLGLA+SGV VAKV H GA+V VNDKKER+ PEA EL +LGISV+ Sbjct: 1 MNHPATYRDRRVVVLGLARSGVSVAKVFHALGAEVIVNDKKERELCPEADELTALGISVL 60 Query: 61 CGGHPEGLIHPGVSLVVKNPGIPYSVPPVQKALELGIEVVTEVEVAYHLCAAPMIGITGS 120 CG HP+ L+ +L+VKNPGIPY+ PVQ+A G+E+VTEVEVAY L AAP+IGITGS Sbjct: 61 CGYHPDDLVTSETALLVKNPGIPYTASPVQQAEAHGVEIVTEVEVAYWLSAAPIIGITGS 120 Query: 121 NGKXXXXXWVGRMLDAAGMHPIVAGNIGTPLCQAAQEAHADNWMVVELSSFQLKGTKAFR 180 NGK W+G++L AG++PIVAGNIG PLC+AA EA DNW+V ELSSFQLKGT +FR Sbjct: 121 NGKTTTTTWIGKLLGEAGLNPIVAGNIGIPLCEAAAEATKDNWIVAELSSFQLKGTSSFR 180 Query: 181 PKVGCLLNVAETHLDYHGGMEDYIASKAKLFANQGPSDTAVLNWDDPVCRELVPYIKAGI 240 P++ LLN+AETHLDYHGGMEDY+ASKAKLFANQ D AVLNWDD CR + + A + Sbjct: 181 PRIALLLNIAETHLDYHGGMEDYVASKAKLFANQTADDVAVLNWDDATCRAIADQLAAKL 240 Query: 241 LPFSMTEELVQGVFVRPAFVPDTEDDLKRMIVYRDYSESETEIAAVDSIGLPGRFNVEXX 300 LPFS+ L QG+ + P F + D + MIV+R +E I V ++GLPGR N Sbjct: 241 LPFSLYVRLDQGICIEPPFERGKDSDKEHMIVWRKEDGTEQAILPVSALGLPGRHNAGNA 300 Query: 301 XXXXXXXXXXXXXXXXXXEVLVNFRGVEHRLEYVAEKAGAAYYNNSKATNSKATSMALAS 360 L FRGVEHRLEY E G YYN+SKATN AT+M++ S Sbjct: 301 LAAIAACLAAGVAPEAMLPALQGFRGVEHRLEYAGEFNGVNYYNDSKATNPMATTMSVGS 360 Query: 361 FRKPVVLIAGGLDRGSDYMELLPVL-GGVKALVALGQTRDKLAAVAGMAGVKRIISVDNG 419 P++LIAGGLDRGSDYMELLPV +K LV LGQTR+KL VA +AG+ + V+ Sbjct: 361 LGAPIILIAGGLDRGSDYMELLPVFRDRLKGLVTLGQTREKLGKVAELAGLTNVRLVEPE 420 Query: 420 ESAAAVLQEAVREASALAEAGDIVLLSPACASWDMFTSYEERGRIFKEAVHNL 472 E A + L+ AVREA+ LAE GDIVLLSPACASWDMFTS+E+RGRIFKE+ H L Sbjct: 421 EDAESTLRRAVREAAGLAEPGDIVLLSPACASWDMFTSFEDRGRIFKESAHTL 473 >C0ZGA7.1 C0ZGA7_BREBN UDP-N-acetylmuramoylalanine--D-glutamate ligase EC=6.3.2.9 Length = 454 Score = 421 bits (1081), Expect = e-115, Method: Compositional matrix adjust. Identities = 231/468 (49%), Positives = 296/468 (63%), Gaps = 22/468 (4%) Query: 6 QYRGKEVVVLGLAKSGVQVAKVLHERGADVTVNDKKERDQSPEASELESLGISVICGGHP 65 ++ K VVV+G+AKSGV VAK+LH GA V VNDKK R+++ ELESLGI VICG HP Sbjct: 3 RFHNKHVVVIGMAKSGVAVAKLLHRFGAHVVVNDKKPREEAVGVEELESLGIPVICGYHP 62 Query: 66 EGLIHPGVSLVVKNPGIPYSVPPVQKALELGIEVVTEVEVAYHLCAAPMIGITGSNGKXX 125 + LIH GVSLVVKNPGIPY PV++A LGI VVTEVE+A + AP+IGITGSNGK Sbjct: 63 DDLIHEGVSLVVKNPGIPYEAAPVKQAQALGIPVVTEVELASQIAKAPIIGITGSNGKTT 122 Query: 126 XXXWVGRMLDAAGMHPIVAGNIGTPLCQAAQEAHADNWMVVELSSFQLKGTKAFRPKVGC 185 VG + AG+ V GNIGT LC A+EA D W+V ELSSFQL GT+ FRP +G Sbjct: 123 TTTLVGLIFKEAGLDAKVGGNIGTVLCGLAEEAGPDEWLVAELSSFQLMGTRKFRPHIGV 182 Query: 186 LLNVAETHLDYHGGMEDYIASKAKLFANQGPSDTAVLNWDDPVCRELVPYIKAGILPFSM 245 LLN+ HLDYH ME+Y+A+K K+FANQ D A+L +D P E + A I FS Sbjct: 183 LLNLYPAHLDYHHTMEEYLAAKLKMFANQTADDAAILPYDQPEIVEKCKDLPASIYYFSK 242 Query: 246 TEELVQGVFVRPAFVPDTEDDLKRMIVYRDYSESETEIAAVDSIGLPGRFNVEXXXXXXX 305 ++E+ +G FV+ +I++ D + + I A+ I +P +++ Sbjct: 243 SQEVPKGAFVKDG-----------LILFADGNGNTEPIVAIADITVP---HLDNALAAIL 288 Query: 306 XXXXXXXXXXXXXEVLVNFRGVEHRLEYVAEKAGAAYYNNSKATNSKATSMALASFRKPV 365 +VL F GVEHR+E+V G Y+N+SKATN +A S AL + ++PV Sbjct: 289 VAKLAGADKQSIIQVLTTFPGVEHRMEFVDSIRGVKYFNDSKATNPEAASRALQACKEPV 348 Query: 366 VLIAGGLDRGSDYMELLPVLGG-VKALVALGQTRDKLAAVAGMAGVKRIISVDNGESAAA 424 V I GGLDRG D+ ELLPV+ G VKA++ALGQT L A AG+ I VD Sbjct: 349 VWICGGLDRGVDFRELLPVIQGRVKAVIALGQTAPILLERAQEAGINERIHVDT------ 402 Query: 425 VLQEAVREASALAEAGDIVLLSPACASWDMFTSYEERGRIFKEAVHNL 472 +++AV AS LAE+GD+VLLSPACASWDMF S+E RG +FK+ VH L Sbjct: 403 -VEKAVLAASQLAESGDVVLLSPACASWDMFPSFETRGSMFKDGVHRL 449 >C5D8M2.1 C5D8M2_9BACI UDP-N-acetylmuramoylalanine/D-glutamate ligase Length = 451 Score = 412 bits (1059), Expect = e-113, Method: Compositional matrix adjust. Identities = 223/473 (47%), Positives = 291/473 (61%), Gaps = 24/473 (5%) Query: 1 MEHPDQYRGKEVVVLGLAKSGVQVAKVLHERGADVTVNDKKERDQSPEASELESLGISVI 60 M+ Y+ + V+V+GLAKSG AK+LHE GA +TVND+K +++ EA +LE LGI VI Sbjct: 1 MKQTAFYQQRRVLVIGLAKSGFAAAKLLHELGAIITVNDQKPFEENKEAQQLEQLGIRVI 60 Query: 61 CGGHPEGLIHPGVSLVVKNPGIPYSVPPVQKALELGIEVVTEVEVAYHLCAAPMIGITGS 120 CGGHP L+ LVVKNPGIPY+ P V+KA+E G+ VVTEVE+AYH+ AP IGITGS Sbjct: 61 CGGHPLELLDEPFDLVVKNPGIPYTNPMVKKAMEKGLPVVTEVELAYHISEAPFIGITGS 120 Query: 121 NGKXXXXXWVGRMLDAAGMHPIVAGNIGTPLCQAAQEAHADNWMVVELSSFQLKGTKAFR 180 NGK + ML P++AGNIGT C+ A++A A NW+V ELSSFQL G + FR Sbjct: 121 NGKTTTTTLIYEMLREGEKRPLLAGNIGTAACEVAKKAEASNWLVTELSSFQLAGIRDFR 180 Query: 181 PKVGCLLNVAETHLDYHGGMEDYIASKAKLFANQGPSDTAVLNWDDPVCRELVPYIKAGI 240 P++ LLN+ + HLDYHG E Y +KA +F NQ D AV+N DD + +L I A Sbjct: 181 PRISVLLNIFDAHLDYHGTKEAYAQAKANIFKNQTNEDYAVINADDELVMQLAENIHAQK 240 Query: 241 LPFSMTEELVQGVFVRPAFVPDTEDDLKRMIVYRDYSESETEIAAVDSIGLPGRFNVEXX 300 + FS T+ L +G ++ F+ Y E IA D I LPG+ N+E Sbjct: 241 VAFSATKVLGRGAYINNGFI---------------YWNDEAVIAVAD-IVLPGKHNLENI 284 Query: 301 XXXXXXXXXXXXXXXXXXEVLVNFRGVEHRLEYVAEKAGAAYYNNSKATNSKATSMALAS 360 +VL F GV+HRL+YVA G ++N+SKATN AT AL++ Sbjct: 285 LAAVAVAKLAGVDNKSIYQVLTTFTGVKHRLQYVATIDGRKFFNDSKATNILATQKALSA 344 Query: 361 FRK-PVVLIAGGLDRGSDYMELLPVLGGVKALVALGQTRDKLAAVAGMAGVKRIISVDNG 419 F K V+L+AGGLDRG+++ LLP L VKA+V GQT K+A VA AG++ I VDN Sbjct: 345 FEKDSVILLAGGLDRGNEFDALLPYLHNVKAVVLFGQTAPKIARVAKQAGIETIEYVDNV 404 Query: 420 ESAAAVLQEAVREASALAEAGDIVLLSPACASWDMFTSYEERGRIFKEAVHNL 472 E A V A L+E GD++LLSPACASWD + ++E+RG IF AVH L Sbjct: 405 EKAVPV-------AYQLSEPGDVILLSPACASWDQYKTFEQRGDIFIGAVHKL 450 >A4IM07.1 A4IM07_GEOTN UDP-N-acetylmuramoylalanine--D-glutamate ligase EC=6.3.2.9 Length = 440 Score = 392 bits (1007), Expect = e-107, Method: Compositional matrix adjust. Identities = 218/462 (47%), Positives = 286/462 (61%), Gaps = 24/462 (5%) Query: 12 VVVLGLAKSGVQVAKVLHERGADVTVNDKKERDQSPEASELESLGISVICGGHPEGLIHP 71 ++V+GLAKSG A++L E G +V ND+K +++ EA +LE LGI V+CGGHP L+ Sbjct: 1 MLVIGLAKSGAAAARLLAELGVEVVANDQKPLEENAEAKKLEQLGIRVVCGGHPLELLDE 60 Query: 72 GVSLVVKNPGIPYSVPPVQKALELGIEVVTEVEVAYHLCAAPMIGITGSNGKXXXXXWVG 131 LVVKNPGIPY+ P V+KALE G+ +VTEVE+AY + AP IGITGSNGK + Sbjct: 61 PFDLVVKNPGIPYTNPLVEKALEKGLPIVTEVELAYRISEAPFIGITGSNGKTTTTTLIY 120 Query: 132 RMLDAAGMHPIVAGNIGTPLCQAAQEAHADNWMVVELSSFQLKGTKAFRPKVGCLLNVAE 191 ML A G P++AGNIG C+ A+EA D W+V ELSSFQL G FRP++ LLN+ + Sbjct: 121 EMLKADGQDPLLAGNIGLVACEVAKEAKPDQWLVTELSSFQLAGIDEFRPRIAVLLNIFD 180 Query: 192 THLDYHGGMEDYIASKAKLFANQGPSDTAVLNWDDPVCRELVPYIKAGILPFSMTEELVQ 251 HLDYHG E Y A+KA +F NQ D AV+N DDP+ ++V ++A + FS+T+ L + Sbjct: 181 AHLDYHGTKEAYAAAKANIFRNQTAHDYAVVNADDPLVMDIVSSVRAQKVLFSVTKPLGE 240 Query: 252 GVFVRPAFVPDTEDDLKRMIVYRDYSESETEIAAVDSIGLPGRFNVEXXXXXXXXXXXXX 311 G +V+ V Y E+ I D I LPG+ N+E Sbjct: 241 GAYVQDGTV---------------YWNGESVIQIAD-IVLPGQHNLENILAAVAAAKLAG 284 Query: 312 XXXXXXXEVLVNFRGVEHRLEYVAEKAGAAYYNNSKATNSKATSMALASF-RKPVVLIAG 370 VL F GV+HRL+YVAE G +YN+SKATN AT ALA+F +PV+L+AG Sbjct: 285 ASNEAIRRVLTTFTGVKHRLQYVAEVDGRRFYNDSKATNILATQKALAAFADEPVILLAG 344 Query: 371 GLDRGSDYMELLPVLGGVKALVALGQTRDKLAAVAGMAGVKRIISVDNGESAAAVLQEAV 430 GLDRG+++ LLP L VKA+V GQT K+ VA AG++ + VDN E A V Sbjct: 345 GLDRGNEFDALLPYLQQVKAMVLFGQTAAKIGRVAREAGIETVEYVDNVEKAVPV----- 399 Query: 431 REASALAEAGDIVLLSPACASWDMFTSYEERGRIFKEAVHNL 472 A L+E GD++LLSPACASWD + ++EERG IF AVH L Sbjct: 400 --AFRLSEPGDVILLSPACASWDQYKTFEERGDIFINAVHKL 439 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,599,566,582 Number of extensions: 101891004 Number of successful extensions: 314443 Number of sequences better than 1.0e-05: 2031 Number of HSP's gapped: 310049 Number of HSP's successfully gapped: 2680 Length of query: 472 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 333 Effective length of database: 1,751,175,118 Effective search space: 583141314294 Effective search space used: 583141314294 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)