BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0043.p (368 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KG89.1 B3KG89_9BACI Stage V sporulation protein E 376 e-102 B1DMA4.1 B1DMA4_9BACL Stage V sporulation protein E 323 1e-86 Q5WFG9.1 Q5WFG9_BACSK Stage V sporulation protein E 306 2e-81 B7GGI3.1 B7GGI3_ANOFW Stage V sporulation protein E required for... 301 5e-80 C3J0A9.1 C3J0A9_9BACI Stage V sporulation protein E 296 2e-78 >B3KG89.1 B3KG89_9BACI Stage V sporulation protein E Length = 365 Score = 376 bits (966), Expect = e-102, Method: Compositional matrix adjust. Identities = 208/365 (56%), Positives = 235/365 (64%) Query: 4 MNKSRHAPDIWILMVILSLLAIGMVMVYSAGSVLGFRNYGDSFYFVKRQXXXXXXXXXXM 63 MNK+R APD+W+L+ IL+LLAIG+VMVYSAGSVL F +YGD FYFVKRQ M Sbjct: 1 MNKNRQAPDLWLLICILALLAIGIVMVYSAGSVLAFHDYGDKFYFVKRQLFFACLGLAAM 60 Query: 64 FVTANTDYLVLKKLARPAXXXXXXXXXXXXXXXXXXXXXXARSWLGISSFGIQPSEFMKM 123 + T+ D+ V KK ++ A ARSWLGISSFGIQPSEFMK+ Sbjct: 61 YFTSRIDFRVWKKYSKIALLVCFFLLIIVLIPGIGVVRGGARSWLGISSFGIQPSEFMKL 120 Query: 124 GMILYLANWLGKEDYDISTFTRGLLPPLALIGSAFALIMMQPDLGTGTVMFGAALMMVFT 183 GMIL+L+ WL K+DYDI++FTRGL+PPL LIG AF LIM+QPDLGTG VM GAA+M+VFT Sbjct: 121 GMILFLSRWLSKQDYDITSFTRGLMPPLGLIGLAFGLIMLQPDLGTGAVMLGAAMMIVFT 180 Query: 184 AGARMKHXXXXXXXXXXXXXXXXXXXPYRLQRITAFLDPWSDPLGAGYQIIQSLYAIXXX 243 AGARMKH PYRL+RIT FLDPWSDPLGAGYQIIQSLYAI Sbjct: 181 AGARMKHLGFLALGGIAGFIGLILTAPYRLKRITGFLDPWSDPLGAGYQIIQSLYAIGPG 240 Query: 244 XXXXXXXXMSRQKYSYVPEPQTDFIFSILAEEXXXXXXXXXXXXXXXXXWRGMKVAMSLP 303 MSRQKY+YVPEPQTDFIFSILAEE WRGM+VAM++ Sbjct: 241 GLGGLGLGMSRQKYAYVPEPQTDFIFSILAEELGFIGGLIVLLLFAALVWRGMRVAMTVT 300 Query: 304 DRFGSYLAVGIVCMXXXXXXXXXXXXXGLMPVTGITLPLISYGGSSXXXXXXXXXXXXXX 363 D FGS LAVGIV M GLMPVTGITLPLISYGGSS Sbjct: 301 DGFGSLLAVGIVGMVAIQVVINIGVVIGLMPVTGITLPLISYGGSSLTLMLTALGILLNI 360 Query: 364 SRYAR 368 SRYAR Sbjct: 361 SRYAR 365 >B1DMA4.1 B1DMA4_9BACL Stage V sporulation protein E Length = 365 Score = 323 bits (829), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 178/365 (48%), Positives = 216/365 (59%) Query: 4 MNKSRHAPDIWILMVILSLLAIGMVMVYSAGSVLGFRNYGDSFYFVKRQXXXXXXXXXXM 63 M K+R APD+W+++ I +L IG+VMVYSA +VL F +GD FY+VKRQ + Sbjct: 1 MAKARSAPDVWMIVSIALILTIGLVMVYSASAVLAFHEFGDKFYYVKRQLIFAVLGVGAL 60 Query: 64 FVTANTDYLVLKKLARPAXXXXXXXXXXXXXXXXXXXXXXARSWLGISSFGIQPSEFMKM 123 T N +YL+ K A+ A ARSWLGISSFGIQPSEFMK+ Sbjct: 61 LFTMNANYLIWKNWAKAALLICFVLLIIVLIPGIGVVRGGARSWLGISSFGIQPSEFMKL 120 Query: 124 GMILYLANWLGKEDYDISTFTRGLLPPLALIGSAFALIMMQPDLGTGTVMFGAALMMVFT 183 MIL+LA WL + I+ FT+GLLPPL L+G AF LIM+QPDLG+G VM GAAL+++F Sbjct: 121 AMILFLAKWLSDKQQTITHFTKGLLPPLGLVGLAFGLIMLQPDLGSGAVMVGAALILIFA 180 Query: 184 AGARMKHXXXXXXXXXXXXXXXXXXXPYRLQRITAFLDPWSDPLGAGYQIIQSLYAIXXX 243 AGAR+ H PYR++RIT FLDPW+DPLG GYQIIQSLYAI Sbjct: 181 AGARISHLALLGSSGLLGLIALVIAEPYRMKRITGFLDPWADPLGTGYQIIQSLYAIGPG 240 Query: 244 XXXXXXXXMSRQKYSYVPEPQTDFIFSILAEEXXXXXXXXXXXXXXXXXWRGMKVAMSLP 303 MSRQK+SY+PEPQTDFIFSI++EE WRG++ A+S P Sbjct: 241 GLVGLGLGMSRQKFSYLPEPQTDFIFSIISEELGFIGGSALIILFMVLVWRGVRTAISAP 300 Query: 304 DRFGSYLAVGIVCMXXXXXXXXXXXXXGLMPVTGITLPLISYGGSSXXXXXXXXXXXXXX 363 D FGS LAVGI + G MPVTGITLPLISYGGSS Sbjct: 301 DTFGSLLAVGITGIIGVQVLINIGVVIGAMPVTGITLPLISYGGSSLTLLLTALGILLNI 360 Query: 364 SRYAR 368 SRY+R Sbjct: 361 SRYSR 365 >Q5WFG9.1 Q5WFG9_BACSK Stage V sporulation protein E Length = 365 Score = 306 bits (783), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 164/365 (44%), Positives = 204/365 (55%) Query: 4 MNKSRHAPDIWILMVILSLLAIGMVMVYSAGSVLGFRNYGDSFYFVKRQXXXXXXXXXXM 63 M K + APD+ +L V ++LL IG++MVYSA + + DSF+F KRQ M Sbjct: 1 MGKQKSAPDLTLLAVTIALLVIGLIMVYSASAAWASYRFSDSFFFAKRQLFFGGTGVLLM 60 Query: 64 FVTANTDYLVLKKLARPAXXXXXXXXXXXXXXXXXXXXXXARSWLGISSFGIQPSEFMKM 123 FV DY V + ++ A+SWLG+ +F IQPSEFMKM Sbjct: 61 FVMMRLDYWVWRTYSKILLIVCFALLVIVLIPGVGLVRGGAQSWLGVGAFSIQPSEFMKM 120 Query: 124 GMILYLANWLGKEDYDISTFTRGLLPPLALIGSAFALIMMQPDLGTGTVMFGAALMMVFT 183 MI++LA +L I T +GL+P L L+ AFALIMMQPDLGTG VM G +MVFT Sbjct: 121 AMIIFLAKFLADHQKWIVTIKKGLVPSLGLVLLAFALIMMQPDLGTGAVMVGTCTVMVFT 180 Query: 184 AGARMKHXXXXXXXXXXXXXXXXXXXPYRLQRITAFLDPWSDPLGAGYQIIQSLYAIXXX 243 AGAR+KH PYR+QRIT+F+DPWSDPLG+G+QIIQSL AI Sbjct: 181 AGARIKHFVALGLIGVFGFVALIASAPYRIQRITSFMDPWSDPLGSGFQIIQSLLAIGPG 240 Query: 244 XXXXXXXXMSRQKYSYVPEPQTDFIFSILAEEXXXXXXXXXXXXXXXXXWRGMKVAMSLP 303 SRQKY Y+PEPQTDFIF+ILAEE WRG+++A+ P Sbjct: 241 GLLGMGLGESRQKYFYLPEPQTDFIFAILAEEMGFLGGVTVLLLFAILYWRGIRIALGAP 300 Query: 304 DRFGSYLAVGIVCMXXXXXXXXXXXXXGLMPVTGITLPLISYGGSSXXXXXXXXXXXXXX 363 D FGS+LA+GI+ M GLMPVTGITLPL+SYGGSS Sbjct: 301 DLFGSFLAIGIITMIAIQVMINIGVVTGLMPVTGITLPLLSYGGSSLTLMLVSIGVLLNI 360 Query: 364 SRYAR 368 SR+AR Sbjct: 361 SRHAR 365 >B7GGI3.1 B7GGI3_ANOFW Stage V sporulation protein E required for spore cortex peptidoglycan synthesis Length = 371 Score = 301 bits (772), Expect = 5e-80, Method: Compositional matrix adjust. Identities = 157/365 (43%), Positives = 204/365 (55%) Query: 4 MNKSRHAPDIWILMVILSLLAIGMVMVYSAGSVLGFRNYGDSFYFVKRQXXXXXXXXXXM 63 ++ + APD ++++ SLLAIG+VMVYSA ++ + DSF+F KRQ M Sbjct: 6 LHGKKSAPDFLLMIITFSLLAIGLVMVYSASAIWADYKFHDSFFFAKRQLLFAGVGIVAM 65 Query: 64 FVTANTDYLVLKKLARPAXXXXXXXXXXXXXXXXXXXXXXARSWLGISSFGIQPSEFMKM 123 F N DY + A+ +RSW+G+ +F IQPSEFMKM Sbjct: 66 FFFMNIDYWTWRTWAKVLLIVCFILLILVLIPGIGMVRNGSRSWIGVGAFSIQPSEFMKM 125 Query: 124 GMILYLANWLGKEDYDISTFTRGLLPPLALIGSAFALIMMQPDLGTGTVMFGAALMMVFT 183 MI +LA +L + I++F +GLLP L L+ AF +IM+QPDLGTGTVM G ++M+F Sbjct: 126 AMIAFLAKYLSENQKKIASFKQGLLPSLTLVFIAFGMIMLQPDLGTGTVMVGTCVVMIFV 185 Query: 184 AGARMKHXXXXXXXXXXXXXXXXXXXPYRLQRITAFLDPWSDPLGAGYQIIQSLYAIXXX 243 AGARM H PYR++RIT+FL+PW DPLG+G+QIIQSLYAI Sbjct: 186 AGARMSHFVLLGLLGLAGFAGLVLSAPYRIKRITSFLNPWEDPLGSGFQIIQSLYAIGPG 245 Query: 244 XXXXXXXXMSRQKYSYVPEPQTDFIFSILAEEXXXXXXXXXXXXXXXXXWRGMKVAMSLP 303 SRQK+ Y+PEPQTDFIF+ILAEE WRG+++A+ P Sbjct: 246 GLFGLGLGQSRQKFFYLPEPQTDFIFAILAEELGFIGGSLVLLLFSLLLWRGIRIALGAP 305 Query: 304 DRFGSYLAVGIVCMXXXXXXXXXXXXXGLMPVTGITLPLISYGGSSXXXXXXXXXXXXXX 363 D +GS+LAVGI+ M GLMPVTGITLP +SYGGSS Sbjct: 306 DLYGSFLAVGIIAMVAIQVMINIGVVTGLMPVTGITLPFLSYGGSSLTLMLMAIGVLLNI 365 Query: 364 SRYAR 368 SRYAR Sbjct: 366 SRYAR 370 >C3J0A9.1 C3J0A9_9BACI Stage V sporulation protein E Length = 366 Score = 296 bits (759), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 153/365 (41%), Positives = 205/365 (56%) Query: 4 MNKSRHAPDIWILMVILSLLAIGMVMVYSAGSVLGFRNYGDSFYFVKRQXXXXXXXXXXM 63 M + + APD ++++ SLLAIG++MVYSA ++ + DSF+F KRQ M Sbjct: 1 MPRKKSAPDFLLIILTFSLLAIGLIMVYSASAIWAEYKFNDSFFFAKRQLLFAGVGIIAM 60 Query: 64 FVTANTDYLVLKKLARPAXXXXXXXXXXXXXXXXXXXXXXARSWLGISSFGIQPSEFMKM 123 F N DY V + ++ +RSW+G+ +F IQPSEFMK+ Sbjct: 61 FFVMNIDYWVWRDWSKVLLGVCFVLLVLVLIPGIGMVRNGSRSWIGVGAFSIQPSEFMKL 120 Query: 124 GMILYLANWLGKEDYDISTFTRGLLPPLALIGSAFALIMMQPDLGTGTVMFGAALMMVFT 183 MI +LA +L + I++F +GLLP L L+ +AF +IM+QPDLGTGTVM G + M+F Sbjct: 121 AMIAFLAKYLSENQKKITSFKQGLLPALLLVFAAFGMIMLQPDLGTGTVMVGTCVTMIFV 180 Query: 184 AGARMKHXXXXXXXXXXXXXXXXXXXPYRLQRITAFLDPWSDPLGAGYQIIQSLYAIXXX 243 AGAR+ H PYR++RIT+FL+PW DPLG+G+QIIQSLYAI Sbjct: 181 AGARLSHFAGLGVLGLAGFAALILSAPYRIKRITSFLNPWEDPLGSGFQIIQSLYAIGPG 240 Query: 244 XXXXXXXXMSRQKYSYVPEPQTDFIFSILAEEXXXXXXXXXXXXXXXXXWRGMKVAMSLP 303 SRQK+ Y+PEPQTDFIF+ILAEE WRG+++A+ P Sbjct: 241 GLFGLGLGQSRQKFFYLPEPQTDFIFAILAEELGFIGGSLVLLLFALLLWRGVRIALGAP 300 Query: 304 DRFGSYLAVGIVCMXXXXXXXXXXXXXGLMPVTGITLPLISYGGSSXXXXXXXXXXXXXX 363 D +GS+LA+GI+ M GLMPVTGITLP +SYGGSS Sbjct: 301 DLYGSFLALGIISMIAIQVMINIGVVTGLMPVTGITLPFLSYGGSSLTLMLMAIGVLLNI 360 Query: 364 SRYAR 368 SR+AR Sbjct: 361 SRHAR 365 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.140 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,462,805,823 Number of extensions: 42297789 Number of successful extensions: 110583 Number of sequences better than 1.0e-05: 3020 Number of HSP's gapped: 103151 Number of HSP's successfully gapped: 3083 Length of query: 368 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 231 Effective length of database: 1,770,017,148 Effective search space: 408873961188 Effective search space used: 408873961188 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)