BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0004.p (495 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1ZXJ4.1 B1ZXJ4_OPITP Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 179 5e-43 B5JDP2.1 B5JDP2_9BACT Glycosyl hydrolase family 26 179 7e-43 B5JPX8.1 B5JPX8_9BACT Glycosyl hydrolase family 26 176 4e-42 Q11Y73.1 Q11Y73_CYTH3 CHU large protein; candidate b-mannanase, ... 158 2e-36 P77847.1 P77847_CALSA Beta-1,4-mannanase EC=3.2.1.78 154 2e-35 >B1ZXJ4.1 B1ZXJ4_OPITP Mannan endo-1,4-beta-mannosidase EC=3.2.1.78 Length = 506 Score = 179 bits (455), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 108/311 (34%), Positives = 162/311 (52%), Gaps = 21/311 (6%) Query: 47 RKLLQTLYRLQGQGMISGQHDYLESPDEFSARLNTTSGQYAALHGYELG-AIENXXXXXX 105 R LL+ LY L G+ ++GQH+Y D S G+ A+ + G A+ Sbjct: 37 RALLELLYDLSGRHTLTGQHNYPNIKDRNSRFAAQHIGKMPAVFSTDFGHAVAGNSDSYL 96 Query: 106 XXXXXAVVDSAIRWHQAGGIVTMSYH------------ENLPGTAP-AWSNVSMSLSEAD 152 +V IR HQ G +VT+ +H LPG+ P A +V L + Sbjct: 97 ARPD--IVQECIRQHQLGALVTICWHAVPPTADEPVTFRPLPGSDPKALKSVQGQLLDEQ 154 Query: 153 FAKYITPGTVQYKALIAELDKTAQSLKKLSNAGVPVLWRPYHEMNGGWFWWGQKS---SF 209 F +TPGT + A++D A LK+L A VPVLWRPYHEMNGGWFWWG ++ S Sbjct: 155 FRDVLTPGTALHTKWAAQVDAIAVFLKQLEAAHVPVLWRPYHEMNGGWFWWGGRTGEYST 214 Query: 210 SKLWNLMFDRYTAYHKLHNLLWVWSPNAKNEWSDEPADYYPGAGKVDVLALDIYEADFKQ 269 +L+ +FDR +H+L NL+WVWS + ++ E ++PG VDVL LD+Y DF Q Sbjct: 215 ERLYRQIFDRLVNHHQLKNLVWVWSMDRVSKPGMEHEKFFPGIDYVDVLGLDVYGNDFAQ 274 Query: 270 SHHDAMWNLGRGKLIAIGENGELPSPAVLAKTQHKWSYQMSWGKLLYEKNSDAVIKAFMN 329 S+++++ L +GK +A+ E G P+ +L + Q KW++ ++W ++ S M Sbjct: 275 SYYESLEKLSQGKPLALAEVGNPPALEILDR-QPKWTFYVTWAGMV-RNTSRKAYATLMA 332 Query: 330 DSFVLTRKEYA 340 D +L + A Sbjct: 333 DPRILNLDDAA 343 >B5JDP2.1 B5JDP2_9BACT Glycosyl hydrolase family 26 Length = 405 Score = 179 bits (454), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 101/252 (40%), Positives = 146/252 (57%), Gaps = 14/252 (5%) Query: 111 AVVDSAIRWHQAGGIVTMSYHENLPGTAPAWSNVSM-----SLSEADFAKYITPGTVQYK 165 + ++ A R H G I+T+ +H P A +S+ S+ ++ + +T GT + Sbjct: 152 STIEEAKRRHAMGHIITLMWHGCYPTDAYPCDGLSVWAEGNLPSDEEWNELVTDGTELNE 211 Query: 166 ALIAELDKTAQSLKKLSNAGVPVLWRPYHEMNGGWFWWGQK---SSFSKLWNLMFDRYTA 222 A +D A LK+L + +PVLWRPYHEMNG WFWWG K F +LW + +D T Sbjct: 212 AWKVGMDVIAVYLKELRDDNIPVLWRPYHEMNGEWFWWGHKKGEDGFKRLWLMTYDYLTN 271 Query: 223 YHKLHNLLWVWSPNAKNEWSDEPA----DYYPGAGKVDVLALDIYEADFKQSHHDAMWNL 278 +H+L+NL+WVW+ NA E DY+PGA VDVLA D+Y D+KQSHHD + L Sbjct: 272 HHELNNLIWVWNANAPRGLPGERGIPYEDYFPGAEYVDVLACDVYRNDYKQSHHDQLVAL 331 Query: 279 GRGKLIAIGENGELPSPAVLAKTQHKWSYQMSWGKLLYEKNSDAVIKAFMNDSFVLTRKE 338 GK +A+GE GE+PS VL K Q +W++ M+WG +L+ N IK + VLT E Sbjct: 332 AEGKPLALGEVGEMPSLEVL-KEQPQWTWMMTWGWILFFSNEAEFIKELYDSEQVLTLDE 390 Query: 339 YAA-KAAQYASM 349 +A +A +Y+ + Sbjct: 391 ISAGEAGRYSVL 402 >B5JPX8.1 B5JPX8_9BACT Glycosyl hydrolase family 26 Length = 323 Score = 176 bits (447), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 108/301 (35%), Positives = 163/301 (54%), Gaps = 14/301 (4%) Query: 47 RKLLQTLYRLQGQGMISGQHDYLESPDEFSARL---NTTSGQYAALHGYELGAIENXXXX 103 R+LL+ LY + G +SGQH P S RL + +Y AL G + G E Sbjct: 11 RELLEFLYSISGDSTLSGQHCV---PLVGSTRLQGIEKSVDRYPALFGQDFGFSEPGTWD 67 Query: 104 XXXXXXXAVVDSAIRWHQAGGIVTMSYHENLP-GTAPAWSNVSM--SLSEADFAKYITPG 160 +VD AIR H+ G ++++ +H P P S+ L++ ++ +TPG Sbjct: 68 GINYRQN-IVDEAIRRHEEGFVISLMWHAVRPIEEEPVRFRESIQGELTDQEWKDLMTPG 126 Query: 161 TVQYKALIAELDKTAQSLKKLSNAGVPVLWRPYHEMNGGWFWWGQK---SSFSKLWNLMF 217 T + +++D A LK+L +A VPVLWRPYHEMNG WFWWG+K + KLW +++ Sbjct: 127 TEVNERWKSQVDVIAFFLKQLRDADVPVLWRPYHEMNGAWFWWGKKGGEDGYEKLWRMLY 186 Query: 218 DRYTAYHKLHNLLWVWSPNAKNEWSDEPADYYPGAGKVDVLALDIYEADFKQSHHDAMWN 277 DR T +HKL NL+WVW+ N + D A YYPG VDVLA D+Y F Q++++ + Sbjct: 187 DRLTHFHKLDNLVWVWNANEVKQGVDSYATYYPGHDVVDVLATDVYSEAFNQANYEELLE 246 Query: 278 LGRGKLIAIGENGELPSPAVLAKTQHKWSYQMSWGKLLYEKNSDAVIKAFMNDSFVLTRK 337 L K IAIGE G++P+ L + Q +W++ M W + ++ ++ + V+T Sbjct: 247 LAGDKPIAIGECGKVPTVEKL-REQPRWAWFMIWERGYEGWDAFGAFRSLFHSEEVITHD 305 Query: 338 E 338 E Sbjct: 306 E 306 >Q11Y73.1 Q11Y73_CYTH3 CHU large protein; candidate b-mannanase, glycoside hydrolase family 26 protein EC=3.2.1.- Length = 815 Score = 158 bits (399), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 91/251 (36%), Positives = 135/251 (53%), Gaps = 20/251 (7%) Query: 115 SAIRWHQAG---GIVTMSYHENLPGTAPAWSNVSMSLSEA-DFAKYITPGTVQYKALIAE 170 +AI W+ + G+V +H + P A +N + S A D K +T GT +Y A+I + Sbjct: 250 AAIDWYNSTCQKGMVAFHWHWHSPSGGQAGTNTFYTNSTAFDVTKAVTQGTPEYTAVIRD 309 Query: 171 LDKTAQSLKKLSNAGVPVLWRPYHEMNGGWFWWGQKSSFS--KLWNLMFDRYTAYHKLHN 228 +D A LK+L AGVPVLWRP HE G WFWWG K + L++++++R YH +HN Sbjct: 310 IDSIATQLKRLQAAGVPVLWRPLHEAGGAWFWWGAKGPAAALALYDMVYNRIVNYHNIHN 369 Query: 229 LLWVWSPNAKNEWSDEPADYYPGAGKVDVLALDIYEADFKQSHH----DAMWNLGRG-KL 283 L+W +WS A++YPG KVD+L D Y + D ++ + G K+ Sbjct: 370 LIW--------QWSTPEANWYPGNSKVDMLGYDSYPGAYNYGTQKLIFDQLYTIVNGQKI 421 Query: 284 IAIGENGELPSPAVLAKTQHKWSYQMSWGKLLYEKNSDAVIKAFMNDSFVLTRKEYAAKA 343 +A+ ENG +P P + + KW+Y MSW L+ ++NS A + N + VLT E A Sbjct: 422 LAMTENGPIPDPDLCFSSDAKWAYVMSWSDLVTQQNSSAQLNLVYNHAKVLTLDE-VTTA 480 Query: 344 AQYASMPEAGP 354 A + AGP Sbjct: 481 TNTAQLTAAGP 491 >P77847.1 P77847_CALSA Beta-1,4-mannanase EC=3.2.1.78 Length = 911 Score = 154 bits (389), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 101/314 (32%), Positives = 157/314 (50%), Gaps = 34/314 (10%) Query: 47 RKLLQTLYRLQGQGMISGQHDYLESPDEFSARLNTTSGQYAALHGYELGAIENXXXXXXX 106 +KL++ L + G+ ++SGQ + E + T +Y A+ G++ ++ Sbjct: 600 QKLMKYLASIYGEKILSGQQSSGDGK-EIQMIFDVTK-RYPAVRGFDF--MDYSPSRVEH 655 Query: 107 XXXXAVVDSAIRWHQAGGIVTMSYHENLPG---TAPA---WSNVSMSLSEADFAKYI-TP 159 V+ AI+W ++GGIV +H N P P W + D K + P Sbjct: 656 GTKGTDVEEAIKWWKSGGIVAFCWHWNAPTGLIDQPGKEWWRGFYTEATTFDIKKAMDNP 715 Query: 160 GTVQYKALIAELDKTAQSLKKLSNAGVPVLWRPYHEMNGGWFWWGQK--SSFSKLWNLMF 217 + +YK ++ ++D A+ LK+L GVPVL+RP HE +GGWFWWG K + KLW LMF Sbjct: 716 NSTEYKLILRDIDAIAEQLKRLQKEGVPVLFRPLHEASGGWFWWGAKGPEPYIKLWKLMF 775 Query: 218 DRYTAYHKLHNLLWVWSPNAKNEWSDEPADYYPGAGKVDVLALDIYEADFKQSHH----- 272 DR YHKL+NL+WV W+ + A +YPG VD++ DIYE + S + Sbjct: 776 DRLVNYHKLNNLIWV--------WNGQDAAWYPGDNYVDIIGEDIYEEKAQYSPYADRFA 827 Query: 273 DAMWNLGRGKLIAIGENGELPSPAVLAKTQHKWS--------YQMSWGKLLYEKNSDAVI 324 A+ K+IA+ E G +P PAVL + WS + M+ K E N + ++ Sbjct: 828 KALKYTNARKMIALTECGTIPDPAVLKQEGISWSWFSVWAGNFVMTGSKYNDEWNDNHML 887 Query: 325 KAFMNDSFVLTRKE 338 + N +V+T+ E Sbjct: 888 RKIYNSDYVITKDE 901 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.131 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,990,058,700 Number of extensions: 121357580 Number of successful extensions: 239333 Number of sequences better than 1.0e-05: 281 Number of HSP's gapped: 238914 Number of HSP's successfully gapped: 303 Length of query: 495 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 355 Effective length of database: 1,741,754,103 Effective search space: 618322706565 Effective search space used: 618322706565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)